##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139830_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12275066
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.107311928098795	33.0	31.0	34.0	30.0	34.0
2	32.261923561144194	34.0	31.0	34.0	30.0	34.0
3	32.31695006772265	34.0	31.0	34.0	30.0	34.0
4	35.95073044821103	37.0	35.0	37.0	35.0	37.0
5	35.83246305966909	37.0	35.0	37.0	35.0	37.0
6	35.84533647313994	37.0	35.0	37.0	35.0	37.0
7	35.767493877426	37.0	35.0	37.0	33.0	37.0
8	35.74796656897812	37.0	35.0	37.0	33.0	37.0
9	37.39689538125498	39.0	37.0	39.0	34.0	39.0
10-11	37.31032655954762	39.0	37.0	39.0	34.0	39.0
12-13	37.291751425206186	39.0	37.0	39.0	34.0	39.0
14-15	38.5328093144265	40.0	38.0	41.0	34.0	41.0
16-17	38.513786076588104	40.0	38.0	41.0	34.0	41.0
18-19	38.547143168110054	40.0	38.0	41.0	34.0	41.0
20-21	38.515480918142096	40.0	38.0	41.0	34.0	41.0
22-23	38.450249715060735	40.0	38.0	41.0	34.0	41.0
24-25	38.3663502892401	40.0	38.0	41.0	34.0	41.0
26-27	38.22829533602663	40.0	38.0	41.0	33.5	41.0
28-29	38.13414993003774	40.0	38.0	41.0	33.0	41.0
30-31	38.02876332929654	40.0	37.0	41.0	33.0	41.0
32-33	38.19842483436133	40.0	38.0	41.0	34.0	41.0
34-35	38.186925538830984	40.0	38.0	41.0	33.0	41.0
36-37	38.10643009387542	40.0	37.5	41.0	33.0	41.0
38-39	37.98352082069654	40.0	37.0	41.0	33.0	41.0
40-41	37.93975711114467	40.0	37.0	41.0	33.0	41.0
42-43	37.86220057248302	40.0	37.0	41.0	33.0	41.0
44-45	37.859501813060774	40.0	37.0	41.0	33.0	41.0
46-47	37.89341008440767	40.0	37.0	41.0	33.0	41.0
48-49	37.81573087638918	40.0	37.0	41.0	33.0	41.0
50-51	37.75141147724662	40.0	37.0	41.0	32.5	41.0
52-53	37.61150650305865	40.0	36.5	41.0	32.0	41.0
54-55	37.49777055321569	39.5	36.0	41.0	32.0	41.0
56-57	37.33101982225996	39.0	36.0	41.0	31.5	41.0
58-59	37.177790904668754	39.0	35.5	41.0	31.0	41.0
60-61	37.02685378025807	39.0	35.0	40.0	31.0	41.0
62-63	36.805542898986744	38.5	35.0	40.0	31.0	41.0
64-65	36.60359298760223	38.0	35.0	40.0	31.0	41.0
66-67	36.349361025609724	37.5	35.0	40.0	31.0	41.0
68-69	36.05917001594551	37.0	34.5	40.0	30.5	41.0
70-71	35.76769737521144	36.5	34.0	39.0	30.0	41.0
72-73	35.44656977975396	36.0	34.0	39.0	30.0	40.5
74-75	35.09014160954874	36.0	34.0	38.5	29.5	40.0
76-77	33.97591825732036	35.0	32.5	37.0	28.5	39.0
78-79	34.286898539768856	35.0	33.0	37.0	29.0	39.0
80-81	34.146903284868856	35.0	33.0	36.5	29.0	39.0
82-83	33.88792817391993	35.0	33.0	36.0	29.0	37.5
84-85	33.68672321319474	35.0	33.0	36.0	29.0	37.0
86-87	33.51587181848392	35.0	33.0	35.0	29.0	37.0
88-89	33.305062223700254	35.0	33.0	35.0	29.0	36.0
90-91	33.2047278245861	35.0	33.0	35.0	29.0	36.0
92-93	33.15687828849026	35.0	33.0	35.0	29.0	36.0
94-95	33.116331125204795	35.0	33.0	35.0	29.0	35.5
96-97	33.05289384329202	34.0	33.0	35.0	29.0	35.0
98-99	33.126788239874166	34.0	33.0	35.0	29.0	35.0
100-101	32.61455112035237	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	5.0
20	52.0
21	339.0
22	1739.0
23	6371.0
24	16843.0
25	36332.0
26	66551.0
27	106353.0
28	154234.0
29	207945.0
30	267607.0
31	335438.0
32	420157.0
33	535313.0
34	711189.0
35	975653.0
36	1490700.0
37	2503527.0
38	3047964.0
39	1331877.0
40	58875.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.52165845788528	21.914480948615672	15.138085611922575	24.425774981576474
2	13.987047966030444	22.841259780423183	40.59909471001718	22.572597543529195
3	16.95847500942154	27.543876342497875	31.56618465432284	23.931463993757752
4	12.244830292562174	19.56824509130949	39.4364641298059	28.75046048632244
5	12.798106340120697	38.25368433864225	36.21493358976644	12.73327573147061
6	28.691666505092517	39.38382897493178	19.23880490744408	12.685699612531614
7	25.17029236339748	33.06222549027435	24.50777861398057	17.259703532347608
8	23.794144976491367	37.06209807751746	21.955719016093276	17.18803792989789
9	25.24554246795903	16.537703341065537	21.692192938107215	36.52456125286822
10-11	23.50904671306859	27.078563162104384	29.93411196322692	19.47827816160011
12-13	24.426581494551638	24.759447321912567	29.686459526979327	21.127511656556468
14-15	21.752636604968153	26.109953298825438	27.068269938426397	25.069140157780005
16-17	21.421514149088893	29.110185639735054	27.73342725815079	21.734872953025263
18-19	21.45284317718941	28.461437881873724	29.18391038696538	20.901808553971488
20-21	22.209237727513916	27.699564844719553	28.919189905485933	21.172007522280598
22-23	21.95527561292924	27.618287995159452	28.77516572773499	21.65127066417632
24-25	21.78133548573542	27.79013867769729	28.78824614382573	21.640279692741558
26-27	21.791838489054754	27.894827840867144	28.705612218502672	21.60772145157543
28-29	21.764043679763855	27.89220412008256	28.73811142038693	21.605640779766656
30-31	21.924104597458907	27.784876302689597	28.658182744948117	21.63283635490337
32-33	21.779894417505343	27.96837553210115	28.56590684153798	21.685823208855524
34-35	21.896510675570664	27.958366311790055	28.5636320049991	21.58149100764018
36-37	21.849770568217142	27.925340934332425	28.55777039342456	21.66711810402587
38-39	21.723660975968393	27.981387298159188	28.616871060740035	21.67808066513238
40-41	21.876605067317513	27.765529029561126	28.558226771034384	21.799639132086973
42-43	21.90564298008878	27.910737569285732	28.361790109426376	21.821829341199113
44-45	21.831866220899514	27.921535144703636	28.222956611813803	22.023642022583047
46-47	21.930752243504063	27.919786744994724	28.026285418592394	22.12317559290882
48-49	21.8219624797363	28.15233668887391	28.02940306999121	21.996297761398584
50-51	21.851173694341718	28.212526994888837	27.957636643770567	21.978662666998883
52-53	21.967844346428333	28.047842553891773	27.660996049582863	22.32331705009703
54-55	21.892530226249367	27.75676806109182	27.89382476603393	22.456876946624885
56-57	21.918932530411983	27.49309760851903	28.17896510189451	22.40900475917448
58-59	21.737254337036404	27.304398502689047	28.304927711370304	22.653419448904245
60-61	21.819979909653483	27.057920642464104	28.61987503244055	22.50222441544186
62-63	21.58184744973319	27.647089599070995	28.566711694668257	22.204351256527556
64-65	21.184251274374315	27.872816657704963	28.3569349100482	22.585997157872523
66-67	21.242080722134954	28.48517484353254	27.861372420461734	22.41137201387077
68-69	21.35380058151228	28.77158611485391	27.399314787408553	22.475298516225262
70-71	21.3776157148672	28.36746851651141	27.386254425022692	22.8686613435987
72-73	21.277382438350642	27.98284908423756	27.283319917052594	23.456448560359203
74-75	21.69919739504597	27.63031041699874	27.375357141065273	23.29513504689002
76-77	22.141973200601708	27.463031985448765	27.280159582809965	23.11483523113956
78-79	22.31884360669213	27.363493498955098	26.557282168076103	23.76038072627667
80-81	22.228689552584864	27.785051433343423	26.497077405713902	23.48918160835781
82-83	22.17206595777231	27.905892946006816	26.559633573812647	23.362407522408223
84-85	22.150741616090304	27.935789334146417	26.538156720221306	23.375312329541977
86-87	22.529442428289855	28.25943460907594	26.415021264702897	22.79610169793131
88-89	22.691113682622905	27.9344453792496	26.791521115164063	22.58291982296343
90-91	22.387090582710393	28.009583405498343	27.059019665120864	22.5443063466704
92-93	22.16084482284326	27.48854327825615	26.925568373963753	23.42504352493684
94-95	21.97876741940759	27.45426320470058	27.629398113406197	22.937571262485633
96-97	21.526927149605914	27.76990507233561	27.922198727520524	22.780969050537948
98-99	21.793765423506155	27.886592747588303	28.29210272703572	22.027539101869824
100-101	22.531539327692244	30.81219455117298	25.557557771716468	21.09870834941832
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3853.0
1	3383.5
2	3962.5
3	5223.5
4	6635.0
5	9833.5
6	12444.0
7	14161.0
8	15094.0
9	14895.5
10	14173.0
11	13353.5
12	14585.5
13	19138.0
14	29355.0
15	43323.0
16	54497.0
17	60235.0
18	61664.0
19	60217.0
20	57100.5
21	54530.5
22	55005.5
23	61179.0
24	73340.5
25	91513.0
26	115138.5
27	140719.5
28	167426.0
29	194041.5
30	219529.5
31	247366.0
32	276480.5
33	304258.0
34	329837.0
35	354457.0
36	383710.5
37	410798.0
38	431687.5
39	450493.5
40	468432.5
41	484078.5
42	495309.5
43	505304.5
44	514561.0
45	521665.0
46	523894.0
47	522288.5
48	518610.5
49	508420.0
50	493839.5
51	474926.0
52	449247.5
53	421608.0
54	392911.5
55	360861.5
56	327109.0
57	292445.5
58	256383.5
59	219441.0
60	182126.5
61	148072.0
62	117990.5
63	90184.0
64	66296.0
65	49173.5
66	36981.5
67	27776.0
68	20955.0
69	15110.5
70	10752.5
71	7788.5
72	5423.5
73	3672.0
74	2504.5
75	1683.5
76	1155.5
77	766.5
78	517.5
79	366.0
80	255.0
81	183.0
82	146.0
83	102.5
84	69.0
85	47.5
86	34.0
87	27.5
88	21.0
89	12.5
90	6.0
91	3.0
92	2.5
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00444804125696758
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.376753167763008E-4
20-21	0.0012057402687187715
22-23	0.002672463740248257
24-25	2.44492301548655E-5
26-27	0.007729040389494718
28-29	1.672419858966057E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.035429199713378975
38-39	0.01649368394184993
40-41	0.025150390647680414
42-43	0.0011495031598915525
44-45	0.005116139337459955
46-47	0.004294116952422258
48-49	0.0025032087274784423
50-51	0.0022598791866834953
52-53	2.3377021605131581E-4
54-55	0.0024826416084195654
56-57	0.01364849605739854
58-59	0.009446255789099313
60-61	0.0033312305218637247
62-63	0.0010652348472028484
64-65	0.0029477823297572813
66-67	2.5268301118393384E-4
68-69	7.114813749056707E-4
70-71	2.5893089129803423E-4
72-73	0.0
74-75	0.0
76-77	0.006805159395348153
78-79	3.7119842698974856E-4
80-81	5.018621343564228E-6
82-83	1.0178721037594393E-4
84-85	0.0017440562382801874
86-87	0.005113106682275685
88-89	0.0768682063863787
90-91	0.0028460152384404526
92-93	0.001302351579865117
94-95	0.0
96-97	0.01802676639066947
98-99	6.597164538681274E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	267.0
20-21	1030.0
22-23	2259.0
24-25	4632.0
26-27	6712.0
28-29	11690.0
30-31	28462.0
32-33	37787.0
34-35	53717.0
36-37	80000.0
38-39	57022.0
40-41	92634.0
42-43	102916.0
44-45	120697.0
46-47	121787.0
48-49	110812.0
50-51	102297.0
52-53	17532.0
54-55	17002.0
56-57	16662.0
58-59	17159.0
60-61	119147.0
62-63	115033.0
64-65	121785.0
66-67	131086.0
68-69	131403.0
70-71	131068.0
72-73	126139.0
74-75	129498.0
76-77	130024.0
78-79	138838.0
80-81	139721.0
82-83	134868.0
84-85	134466.0
86-87	141993.0
88-89	149271.0
90-91	158555.0
92-93	165331.0
94-95	205852.0
96-97	268377.0
98-99	1116568.0
100-101	7382967.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.79667965433049
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.047949411158	74.61922490681833
2	7.507402297955731	12.581907707959619
3	1.8916289203756826	4.7553666799676435
4	0.6879953831497938	2.3060691490184655
5	0.32698555280288427	1.3700151809908727
6	0.18272019303295	0.9186807289175724
7	0.09960400040843653	0.584253916016089
8	0.0625509629435216	0.4193250403078541
9	0.039273498106356566	0.29618898657509846
>10	0.1508783953708746	1.9229334147214843
>50	0.002415223219298816	0.1324437255328586
>100	5.630232616132118E-4	0.0738053839656935
>500	3.31382148594053E-5	0.019785179208418033
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.14659570873183E-6	0.0
2	0.0	8.14659570873183E-6	0.0	8.14659570873183E-6	0.0
3	0.0	8.14659570873183E-6	0.0	8.14659570873183E-6	0.0
4	0.0	4.073297854365916E-5	0.0	2.4439787126195492E-5	0.0
5	0.0	4.8879574252390984E-5	0.0	3.258638283492732E-5	0.0
6	0.0	4.8879574252390984E-5	0.0	3.258638283492732E-5	8.14659570873183E-6
7	0.0	4.8879574252390984E-5	0.0	3.258638283492732E-5	8.14659570873183E-6
8	0.0	4.8879574252390984E-5	0.0	3.258638283492732E-5	8.14659570873183E-6
9	0.0	1.6293191417463663E-4	0.0	4.8879574252390984E-5	8.14659570873183E-6
10-11	0.0	1.6293191417463663E-4	0.0	6.517276566985465E-5	8.14659570873183E-6
12-13	0.0	1.6700521202900255E-4	0.0	8.961255279605014E-5	8.14659570873183E-6
14-15	0.0	2.1995808413575942E-4	0.0	1.0997904206787971E-4	8.14659570873183E-6
16-17	0.0	2.2810467984449127E-4	0.0	1.1812563777661155E-4	8.14659570873183E-6
18-19	0.0	2.647643605337845E-4	0.0	1.303455313397093E-4	1.629319141746366E-5
20-21	0.0	2.932774455143459E-4	0.0	1.588586163202707E-4	1.629319141746366E-5
22-23	0.0	3.3401042405800506E-4	0.0	2.3625127555322313E-4	1.629319141746366E-5
24-25	0.0	3.82889998310396E-4	0.0	3.217905304949073E-4	1.629319141746366E-5
26-27	0.0	4.0732978543659153E-4	0.0	5.82481593174326E-4	1.629319141746366E-5
28-29	0.0	4.0732978543659153E-4	0.0	0.0022484604156099855	1.629319141746366E-5
30-31	0.0	4.399161682715189E-4	0.0	0.007637433476936091	1.629319141746366E-5
32-33	0.0	4.5213606183461665E-4	0.0	0.02016282437911128	1.629319141746366E-5
34-35	0.0	5.010156360870076E-4	0.0	0.037181062814652076	2.4439787126195492E-5
36-37	0.0	5.498952103393986E-4	0.0	0.058614756124325526	2.4439787126195492E-5
38-39	0.0	5.621151039024964E-4	0.0	0.10441899049667024	3.258638283492732E-5
40-41	0.0	6.476543588441806E-4	0.0	0.412054810947656	4.073297854365916E-5
42-43	0.0	6.72094145970376E-4	0.0	0.45187129747408283	4.073297854365916E-5
44-45	0.0	7.454135073489625E-4	0.0	0.5177120839920535	4.073297854365916E-5
46-47	0.0	8.146595708731831E-4	0.0	0.5929784817450269	4.8879574252390984E-5
48-49	0.0	8.146595708731831E-4	0.0	0.6552429127468642	5.702616996112282E-5
50-51	0.0	8.716857408343059E-4	0.0	0.7146519619527911	5.702616996112282E-5
52-53	0.0	8.798323365430378E-4	0.0	0.9331069991802896	6.109946781548873E-5
54-55	0.0	8.839056343974037E-4	0.0	1.6829807676797828	6.517276566985465E-5
56-57	0.0	9.042721236692332E-4	0.0	2.510340881262879	6.924606352422056E-5
58-59	0.0	9.16492017232331E-4	0.0	3.370307744170174	7.331936137858647E-5
60-61	0.0	9.409318043585265E-4	0.0	4.02030017598276	7.331936137858647E-5
62-63	0.0	9.653715914847219E-4	0.0	4.037159555801981	7.331936137858647E-5
64-65	0.0	0.0011405233992224564	0.0	4.0476279312877015	7.331936137858647E-5
66-67	0.0	0.001152743292785554	0.0	4.055864139549229	7.331936137858647E-5
68-69	0.0	0.0011812563777661156	0.0	4.063163489304253	7.331936137858647E-5
70-71	0.0	0.0012016228670379452	0.0	4.070405812889316	7.331936137858647E-5
72-73	0.0	0.0012260626541641404	0.0	4.076039183821903	8.146595708731831E-5
74-75	0.0	0.0012627223348534338	0.0	4.081314104543308	8.146595708731831E-5
76-77	0.0	0.0013197485048145566	0.0	4.086047276650081	8.146595708731831E-5
78-79	0.0	0.0013360416962320202	0.0	4.0901490875894275	8.146595708731831E-5
80-81	0.0	0.001340114994086386	0.0	4.092495307153541	8.146595708731831E-5
82-83	0.0	0.001348261589795118	0.0	4.094197945656667	9.775914850478197E-5
84-85	0.0	0.0013604814833582158	0.0	4.095949463734044	1.0183244635914788E-4
86-87	0.0	0.0013686280790669476	0.0	4.096320133838791	1.0997904206787971E-4
88-89	0.0	0.0014093610576106066	0.0	4.0963853066044615	1.1405233992224564E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12780	0.0	46.39223	1
TATCTCG	15655	0.0	36.52929	94-95
GTATCAA	22235	0.0	34.900417	1
CCGTCTT	6285	0.0	32.428307	92-93
TATGCCG	6390	0.0	30.464985	88-89
TGCCGTC	6685	0.0	29.489347	90-91
GCCGTCT	6500	0.0	29.447554	90-91
TCAACGC	26980	0.0	28.033083	4
ATCAACG	27485	0.0	27.611187	3
ATGCCGT	6810	0.0	27.549736	88-89
CAACGCA	27830	0.0	27.238226	5
AACGCAG	28610	0.0	26.555302	6
CGTATGC	7345	0.0	26.441872	86-87
TATCAAC	28930	0.0	26.410612	2
CTCGTAT	7615	0.0	25.348303	84-85
TCGTATG	7370	0.0	25.306385	84-85
GCTATCT	24745	0.0	24.819996	94-95
CTATCTC	24550	0.0	24.80709	94-95
TCTCGTA	7800	0.0	23.845652	82-83
ATCTCGT	12435	0.0	22.807499	94-95
>>END_MODULE