FastQCFastQC Report
Fri 27 May 2016
SRR2139829_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139829_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10316241
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133450.050.4795461
GTATCAA235900.033.9933741
TCAACGC328850.026.3664593
ATCAACG331000.026.1069032
TATCAAC337150.025.780351
CAACGCA337450.025.7194234
AACGCAG344050.025.2504865
ACGCAGA404100.021.519036
CGCAGAG408350.021.274477
CAGAGTA457050.018.9984159
AGAGTAC429500.018.75829310-11
GCAGAGT462300.018.682618
AGTACTT338200.017.62432712-13
GAGTACT311250.017.00227212-13
TGGTATC57850.016.3550032
GTGGTAT63300.016.2090531
GTACTTT340450.015.52675814-15
GTACATG242800.014.9289781
TATACTG66000.014.781045
TACATGG248950.014.7121972
Produced by FastQC (version 0.11.5)