##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139829_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10316241
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.187127753219414	31.0	31.0	34.0	30.0	34.0
2	31.411117479709905	31.0	31.0	34.0	30.0	34.0
3	31.402025020547697	31.0	31.0	34.0	30.0	34.0
4	35.218637098532305	35.0	35.0	37.0	33.0	37.0
5	34.930533999738856	35.0	35.0	37.0	32.0	37.0
6	34.98859710625217	35.0	35.0	37.0	32.0	37.0
7	34.88511396738405	35.0	35.0	37.0	32.0	37.0
8	34.87073886699623	35.0	35.0	37.0	32.0	37.0
9	36.33151018864332	38.0	35.0	39.0	32.0	39.0
10-11	36.1287966711906	38.0	35.0	39.0	32.0	39.0
12-13	36.075007020483525	37.0	35.0	39.0	31.0	39.0
14-15	37.01703910368127	38.0	36.0	40.0	31.0	41.0
16-17	36.97899176647773	38.0	36.0	40.0	31.0	41.0
18-19	36.99341610960814	38.5	36.0	40.0	31.0	41.0
20-21	36.96668929023718	39.0	36.0	40.0	31.0	41.0
22-23	36.87954940628423	39.0	36.0	40.0	31.0	41.0
24-25	36.77236441718371	38.0	35.5	40.0	31.0	41.0
26-27	36.51942830727238	38.0	35.0	40.0	30.0	41.0
28-29	36.361320413018305	38.0	35.0	40.0	30.0	41.0
30-31	36.17311248618472	38.0	35.0	40.0	30.0	41.0
32-33	36.35610956490206	38.0	35.0	40.0	30.0	41.0
34-35	36.28243635381209	38.0	35.0	40.0	30.0	41.0
36-37	36.16443297870569	38.0	35.0	40.0	30.0	41.0
38-39	36.01406193937349	38.0	34.5	40.0	30.0	41.0
40-41	35.892521460902245	38.0	34.0	40.0	29.0	41.0
42-43	35.80831218056219	38.0	34.0	40.0	29.0	41.0
44-45	35.83786789668941	38.0	34.0	40.0	29.0	41.0
46-47	35.96280301161286	38.0	34.0	40.0	29.0	41.0
48-49	35.900111147166925	38.0	34.0	40.0	29.0	41.0
50-51	35.78378834905085	38.0	34.0	40.0	28.0	41.0
52-53	35.65934165419878	38.0	34.0	40.0	28.0	41.0
54-55	35.50884898363417	38.0	34.0	40.0	28.0	41.0
56-57	35.363870214654796	38.0	33.0	40.0	28.0	41.0
58-59	35.20815404812303	37.0	33.0	40.0	27.5	41.0
60-61	34.98494925026688	37.0	33.0	39.5	27.0	40.5
62-63	34.75598934245137	37.0	33.0	39.0	27.0	40.0
64-65	34.53998452476215	36.0	32.0	39.0	26.0	40.0
66-67	34.285388413230805	36.0	32.0	39.0	26.0	40.0
68-69	34.00377117708754	35.5	32.0	38.5	26.0	40.0
70-71	33.717013354855354	35.0	31.0	38.0	26.0	40.0
72-73	33.41146368599709	35.0	31.0	38.0	26.0	40.0
74-75	33.06640020570529	34.5	31.0	37.0	26.0	39.0
76-77	32.220928822692684	34.0	30.0	36.0	25.5	39.0
78-79	32.67792373631867	34.0	31.0	36.0	26.0	39.0
80-81	32.58945698763515	34.0	31.0	36.0	26.0	38.5
82-83	32.36129927274051	34.0	31.0	35.5	26.0	37.0
84-85	32.13004915207285	34.0	31.0	35.0	26.0	37.0
86-87	31.896434730090014	34.0	30.0	35.0	25.0	36.5
88-89	31.677672423752483	34.0	30.0	35.0	25.0	36.0
90-91	31.549159695958878	34.0	30.0	35.0	25.0	36.0
92-93	31.465940591472553	34.0	30.0	35.0	25.0	35.0
94-95	31.44876436476143	34.0	30.0	35.0	25.0	35.0
96-97	31.497095595827986	34.0	30.0	35.0	25.0	35.0
98-99	31.721628142439563	34.0	30.0	35.0	25.5	35.0
100-101	31.151735375718516	33.0	30.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	9.0
19	35.0
20	227.0
21	1341.0
22	5614.0
23	17117.0
24	41333.0
25	81273.0
26	136973.0
27	206505.0
28	281533.0
29	359757.0
30	443728.0
31	536486.0
32	647286.0
33	791854.0
34	984904.0
35	1238892.0
36	1559932.0
37	1684118.0
38	1076275.0
39	219512.0
40	1536.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.351051221079462	26.977888554561684	18.82415309995181	22.84690712440704
2	14.315559439881568	26.55915450251374	38.16232368343708	20.962962374167613
3	16.77036238296488	29.41622825600914	32.93564002624599	20.877769334779984
4	13.273003218905025	22.83024407824517	37.743166333551144	26.153586369298665
5	11.90899863622806	39.670089134210805	36.54981499559772	11.871097233963418
6	27.557673381224806	42.12889171549986	18.310070499516247	12.003364403759083
7	23.591325561316374	34.13151166204822	25.155461180094573	17.121701596540834
8	23.232890739950722	38.81164660654981	21.55240460163736	16.403058051862107
9	24.622156461835274	16.17846073972099	21.91708200690542	37.28230079153831
10-11	23.147210306544796	28.14898857054619	30.097653786878382	18.606147336030634
12-13	23.99041957240045	25.707769913479144	30.162236419253873	20.139574094866532
14-15	21.14055400605705	27.08715315976042	27.741117137531006	24.031175696651523
16-17	20.619913784487974	29.917641513027853	28.52076158360395	20.94168311888022
18-19	20.616125585286493	29.000291143786015	30.43219803089478	19.951385240032714
20-21	21.299481763419735	28.163935239912963	30.346935823702232	20.18964717296507
22-23	21.003684229178806	28.202269432426636	30.2450184754448	20.54902786294976
24-25	20.91853694207582	28.38909830938283	30.164199289850973	20.528165458690374
26-27	20.98171705298249	28.426967377180308	30.09922299768084	20.492092572156366
28-29	20.915189799771998	28.49351952137284	30.154931037132027	20.436359641723133
30-31	21.167185457360414	28.347172479286257	30.04878812552409	20.436853937829238
32-33	21.107690757453604	28.467588440499668	29.96053090758058	20.464189894466152
34-35	21.135696002532523	28.591555813772906	29.985538557875653	20.28720962581892
36-37	21.09712201257664	28.52606179463547	29.917013454327112	20.459802738460777
38-39	21.115649042974212	28.55367626039663	29.8844162641492	20.446258432479965
40-41	21.30954916466812	28.52192482571967	29.714759985010197	20.45376602460202
42-43	21.204864541019887	28.695931259253094	29.438200144441673	20.661004055285346
44-45	21.30362959828493	28.779138243144907	29.164317917766073	20.752914240804085
46-47	21.44919975101585	28.788871063580885	28.86386419907183	20.898064986331427
48-49	21.34476031035704	28.933167637873865	28.81034351759441	20.91172853417469
50-51	21.449986069237706	28.99689470266266	28.61811410629717	20.935005121802458
52-53	21.542542339367067	28.933248303217045	28.5803698286259	20.943839528789983
54-55	21.509560949116395	28.941940784105668	28.59571886387159	20.952779402906348
56-57	21.530885679837404	28.95421844938852	28.524590810577223	20.990305060196857
58-59	21.52284760651888	29.077504669328846	28.53230919369348	20.86733853045879
60-61	21.64681666731269	29.062423567685265	28.472726938636093	20.81803282636595
62-63	21.56422599220012	29.07371774807337	28.43510406610677	20.926952193619737
64-65	21.491903538114645	29.047480522789375	28.456066424893677	21.0045495142023
66-67	21.565603722226925	29.013395454971043	28.388100690504082	21.03290013229795
68-69	21.5400938926311	28.938289227424356	28.44638851551299	21.075228364431553
70-71	21.53229135812542	28.784653700728086	28.494607932001077	21.188447009145413
72-73	21.5194455407574	28.87829886773625	28.467621715560153	21.13463387594619
74-75	21.471364848795037	28.930286859909565	28.41788343334567	21.18046485794973
76-77	21.518992327845933	28.85288790344966	28.445917762429346	21.182202006275062
78-79	21.500547796218356	28.84285642027574	28.442724378032874	21.213871405473025
80-81	21.573376956853995	28.852431523931916	28.41489604808252	21.159295471131568
82-83	21.51735301209113	28.785469845571733	28.58193393814893	21.115243204188207
84-85	21.553760734975093	28.82654579137467	28.470509560758394	21.149183912891843
86-87	21.486366045026305	28.82176878020258	28.4987386254067	21.193126549364415
88-89	21.518238552215934	28.756078913766842	28.499890357303485	21.225792176713735
90-91	21.507840466957475	28.77581586000315	28.617078890007473	21.0992647830319
92-93	21.537917660505418	28.661176466045468	28.619970311710492	21.18093556173862
94-95	21.248181883020962	28.756739762746157	28.72065952182326	21.274418832409623
96-97	21.134800659654402	28.90026852979349	28.792231850368317	21.172698960183798
98-99	21.181577404235835	29.50832083406939	28.896592305385717	20.413509456309058
100-101	21.788637643858845	32.31868360080015	26.263649495779585	19.629029259561428
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4502.0
1	3816.0
2	4388.5
3	5871.5
4	7026.0
5	9201.0
6	11182.0
7	14060.5
8	16797.0
9	17535.0
10	16508.5
11	15469.5
12	17815.5
13	24977.0
14	42342.0
15	66306.0
16	82777.0
17	87011.5
18	85037.0
19	81521.5
20	76943.0
21	72664.5
22	71669.0
23	77989.5
24	91775.5
25	112728.0
26	138569.5
27	165685.0
28	193954.0
29	221896.5
30	246558.5
31	269835.0
32	292386.5
33	313531.5
34	331913.5
35	348312.0
36	367244.5
37	383591.5
38	394645.5
39	400749.0
40	405226.0
41	411380.5
42	413617.5
43	412884.5
44	411676.0
45	408456.5
46	403865.0
47	398648.5
48	392822.5
49	381452.5
50	365829.0
51	346387.0
52	322914.5
53	298850.5
54	273165.5
55	247394.0
56	222880.5
57	198610.5
58	172986.0
59	145063.0
60	118008.0
61	94753.5
62	74596.5
63	57214.5
64	42171.0
65	30851.5
66	22862.5
67	17395.0
68	13517.0
69	10069.5
70	7373.0
71	5461.5
72	3997.5
73	2872.0
74	2029.0
75	1420.0
76	1008.5
77	699.5
78	482.5
79	351.0
80	268.5
81	200.0
82	155.0
83	120.0
84	90.5
85	62.0
86	42.0
87	31.0
88	19.5
89	12.5
90	8.5
91	5.5
92	4.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0013280030972521871
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	3.150372310999714E-4
20-21	4.701701516696203E-4
22-23	2.133166713620575E-4
24-25	1.2124528216420308E-4
26-27	0.002455413976730731
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.02717242881321755
38-39	0.010399682451841375
40-41	0.019138494551017772
42-43	0.006569648025978379
44-45	0.0022262766371349304
46-47	0.004085637825192033
48-49	0.008184514075252409
50-51	0.0061518119632167365
52-53	0.009762669197558348
54-55	0.0020912934068806725
56-57	0.01468851788004608
58-59	0.011538108423992334
60-61	0.011007487918398692
62-63	0.003937125262687798
64-65	0.005414936425262032
66-67	2.8610338631968046E-4
68-69	9.08172468081853E-5
70-71	4.941423731049568E-4
72-73	4.654156603992242E-5
74-75	7.128118264554159E-4
76-77	7.937615356528438E-4
78-79	6.776315518803187E-4
80-81	1.597178547238337E-4
82-83	0.001847455245864774
84-85	0.0031082266767550825
86-87	9.42447506480779E-4
88-89	0.061474043718634075
90-91	6.322266363000591E-4
92-93	6.8952730076470644E-6
94-95	4.258074817922947E-4
96-97	0.009061175309579107
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	473.0
20-21	1813.0
22-23	3098.0
24-25	5370.0
26-27	7524.0
28-29	10715.0
30-31	19716.0
32-33	26877.0
34-35	39424.0
36-37	55715.0
38-39	62805.0
40-41	69680.0
42-43	84769.0
44-45	99933.0
46-47	98685.0
48-49	86717.0
50-51	77659.0
52-53	76659.0
54-55	84674.0
56-57	90109.0
58-59	92179.0
60-61	93533.0
62-63	92219.0
64-65	97510.0
66-67	103154.0
68-69	106207.0
70-71	108232.0
72-73	106226.0
74-75	109900.0
76-77	110513.0
78-79	122766.0
80-81	128578.0
82-83	127871.0
84-85	133799.0
86-87	145441.0
88-89	158218.0
90-91	179711.0
92-93	195571.0
94-95	243745.0
96-97	329978.0
98-99	905308.0
100-101	5623167.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.14611098277614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.35225134092104	75.1864445789405
2	7.199762392260521	12.116640106175431
3	1.8666840893980254	4.71222619668806
4	0.7249308073497774	2.440004326803515
5	0.3308617601692601	1.3920365195579611
6	0.18680989940413195	0.9431595916764799
7	0.09699483021595968	0.571321642367537
8	0.0685394440990792	0.4613862135887119
9	0.03637366531175078	0.2754632230355642
>10	0.13391449922106274	1.678568654183408
>50	0.002401579012749108	0.13122832743045498
>100	4.371719109486976E-4	0.07178083958703722
>500	3.8520725753143764E-5	0.019739779965277635
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.9080359793843515E-5	0.0	0.0	0.0
3	0.0	2.9080359793843515E-5	0.0	0.0	0.0
4	0.0	5.816071958768703E-5	0.0	9.693453264614505E-6	0.0
5	0.0	5.816071958768703E-5	0.0	9.693453264614505E-6	0.0
6	0.0	5.816071958768703E-5	0.0	9.693453264614505E-6	0.0
7	0.0	5.816071958768703E-5	0.0	9.693453264614505E-6	0.0
8	0.0	5.816071958768703E-5	0.0	1.938690652922901E-5	0.0
9	0.0	1.2601489243998856E-4	0.0	1.938690652922901E-5	0.0
10-11	0.0	1.2601489243998856E-4	0.0	2.9080359793843515E-5	0.0
12-13	0.0	1.308616190722958E-4	0.0	2.9080359793843515E-5	0.0
14-15	0.0	2.0840924518921185E-4	0.0	2.9080359793843515E-5	0.0
16-17	0.0	2.132559718215191E-4	0.0	6.300744621999428E-5	0.0
18-19	4.8467266323072525E-6	2.3264287835074812E-4	0.0	6.785417285230153E-5	0.0
20-21	1.4540179896921757E-5	2.617232381445916E-4	0.0	7.270089948460879E-5	0.0
22-23	1.938690652922901E-5	3.0049705120304966E-4	0.0	8.724107938153054E-5	0.0
24-25	1.938690652922901E-5	3.635044974230439E-4	0.0	1.0662798591075955E-4	0.0
26-27	1.938690652922901E-5	3.780446773199657E-4	0.0	1.6963543213075383E-4	0.0
28-29	1.938690652922901E-5	3.780446773199657E-4	0.0	6.300744621999428E-4	0.0
30-31	1.938690652922901E-5	3.974315838491947E-4	0.0	0.002093785905156733	0.0
32-33	1.938690652922901E-5	4.1197176374611644E-4	0.0	0.004909734078527246	0.0
34-35	1.938690652922901E-5	4.507455768045745E-4	0.0	0.009611058911865282	0.0
36-37	1.938690652922901E-5	4.8467266323072523E-4	0.0	0.01605235860620162	0.0
38-39	1.938690652922901E-5	5.040595697599542E-4	0.0	0.025653724064802284	0.0
40-41	1.938690652922901E-5	5.428333828184122E-4	0.0	0.035933631251925974	0.0
42-43	1.938690652922901E-5	5.719137426122558E-4	0.0	0.04463350555691749	0.0
44-45	1.938690652922901E-5	6.591548219937863E-4	0.0	0.05572766281826878	0.0
46-47	1.938690652922901E-5	7.173155415814733E-4	0.0	0.06824675770951842	0.0
48-49	1.938690652922901E-5	7.173155415814733E-4	0.0	0.08063499098169576	0.0
50-51	1.938690652922901E-5	7.706295345368531E-4	0.0	0.09247554414442238	0.0
52-53	1.938690652922901E-5	7.754762611691604E-4	0.0	0.10367632939168443	0.0
54-55	1.938690652922901E-5	7.754762611691604E-4	0.0	0.11568651798654181	0.0
56-57	2.4233633161536262E-5	7.754762611691604E-4	0.0	0.12796327654617606	0.0
58-59	2.9080359793843515E-5	7.754762611691604E-4	0.0	0.14007524640031188	0.0
60-61	2.9080359793843515E-5	8.142500742276184E-4	0.0	0.1517073903178493	0.0
62-63	2.9080359793843515E-5	8.336369807568474E-4	0.0	0.1626125252405406	0.0
64-65	2.9080359793843515E-5	9.499584199322215E-4	0.0	0.17329471073814579	0.0
66-67	2.9080359793843515E-5	9.693453264614505E-4	0.0	0.18406413731513252	0.0
68-69	2.9080359793843515E-5	0.0010032724128876012	0.0	0.19570597468593454	0.0
70-71	2.9080359793843515E-5	0.001017812592784523	0.0	0.2064511676297597	0.0
72-73	2.9080359793843515E-5	0.001017812592784523	0.0	0.21390058646361598	0.0
74-75	2.9080359793843515E-5	0.0010614331324752882	0.0	0.2204097403308046	0.0
76-77	2.9080359793843515E-5	0.0011292873053275897	0.0	0.2256926723600195	0.0
78-79	2.9080359793843515E-5	0.001153520938489126	0.0	0.23038430374009294	0.0
80-81	2.9080359793843515E-5	0.001153520938489126	0.0	0.23349590223803418	0.0
82-83	2.9080359793843515E-5	0.0011583676651214332	0.0	0.2349159931413002	0.0
84-85	2.9080359793843515E-5	0.0011777545716506624	0.0	0.2363215438646693	0.0
86-87	2.9080359793843515E-5	0.0011922947515475841	0.0	0.23682075670779695	0.0
88-89	2.9080359793843515E-5	0.0012213751113414276	0.0	0.23692738469370772	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13345	0.0	50.479546	1
GTATCAA	23590	0.0	33.993374	1
TCAACGC	32885	0.0	26.366459	3
ATCAACG	33100	0.0	26.106903	2
TATCAAC	33715	0.0	25.78035	1
CAACGCA	33745	0.0	25.719423	4
AACGCAG	34405	0.0	25.250486	5
ACGCAGA	40410	0.0	21.51903	6
CGCAGAG	40835	0.0	21.27447	7
CAGAGTA	45705	0.0	18.998415	9
AGAGTAC	42950	0.0	18.758293	10-11
GCAGAGT	46230	0.0	18.68261	8
AGTACTT	33820	0.0	17.624327	12-13
GAGTACT	31125	0.0	17.002272	12-13
TGGTATC	5785	0.0	16.355003	2
GTGGTAT	6330	0.0	16.209053	1
GTACTTT	34045	0.0	15.526758	14-15
GTACATG	24280	0.0	14.928978	1
TATACTG	6600	0.0	14.78104	5
TACATGG	24895	0.0	14.712197	2
>>END_MODULE