FastQCFastQC Report
Fri 27 May 2016
SRR2139828_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139828_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9600822
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112300.045.4675941
GTATCAA193350.031.9495031
TCAACGC231950.026.0180474
ATCAACG233700.025.7874013
CAACGCA237000.025.3930195
AACGCAG242700.024.8483776
TATCAAC252650.024.0198672
ACGCAGA281800.021.4451967
CGCAGAG283250.021.320648
GTACATG235250.018.9292721
TACATGG234750.018.3990752
AGAGTAC306400.017.57897610-11
ACATGGG239750.017.5607533
GCAGAGT340450.017.4926419
GAGTACT205150.017.26868212-13
CAGAGTA335550.016.43225510-11
CATGGGG171050.015.7078144
AGTACTT231550.015.33595712-13
GTGGTAT62450.015.2800331
GTACTTT238450.014.92837214-15
Produced by FastQC (version 0.11.5)