##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139828_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9600822
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.955976165374175	31.0	31.0	34.0	30.0	34.0
2	32.12080663509854	33.0	31.0	34.0	30.0	34.0
3	32.153410822531654	33.0	31.0	34.0	30.0	34.0
4	35.856161691155194	37.0	35.0	37.0	35.0	37.0
5	35.68939315821083	37.0	35.0	37.0	33.0	37.0
6	35.702131650810735	37.0	35.0	37.0	33.0	37.0
7	35.60595832315192	37.0	35.0	37.0	33.0	37.0
8	35.59127468460513	37.0	35.0	37.0	33.0	37.0
9	37.17190965523577	39.0	37.0	39.0	33.0	39.0
10-11	37.05887954177257	39.0	37.0	39.0	33.0	39.0
12-13	37.01423346875924	39.0	37.0	39.0	33.0	39.0
14-15	38.17809396945387	40.0	37.0	41.0	33.0	41.0
16-17	38.16206877911079	40.0	37.0	41.0	33.0	41.0
18-19	38.185668581294394	40.0	37.5	41.0	33.0	41.0
20-21	38.16186672195297	40.0	37.0	41.0	33.0	41.0
22-23	38.09883044037556	40.0	37.0	41.0	33.0	41.0
24-25	38.0182436707315	40.0	37.0	41.0	33.0	41.0
26-27	37.85008961595424	40.0	37.0	41.0	33.0	41.0
28-29	37.74796200414278	39.5	37.0	41.0	32.0	41.0
30-31	37.60422162151024	39.0	37.0	41.0	32.0	41.0
32-33	37.780717475698	39.5	37.0	41.0	33.0	41.0
34-35	37.73999867848363	40.0	37.0	41.0	33.0	41.0
36-37	37.65534937399853	40.0	37.0	41.0	32.5	41.0
38-39	37.557016216765916	39.0	36.0	41.0	32.0	41.0
40-41	37.46638915980815	39.0	36.0	41.0	32.0	41.0
42-43	37.4262871430676	39.0	36.0	41.0	32.0	41.0
44-45	37.44944114435518	39.0	36.5	41.0	32.0	41.0
46-47	37.55215273334619	40.0	37.0	41.0	32.0	41.0
48-49	37.502111307006494	39.5	36.0	41.0	32.0	41.0
50-51	37.40114550070385	39.0	36.0	41.0	32.0	41.0
52-53	37.31160828348015	39.0	36.0	41.0	31.0	41.0
54-55	37.18907924705746	39.0	36.0	40.5	31.0	41.0
56-57	37.06507625834161	39.0	35.5	40.0	31.0	41.0
58-59	36.92874232086427	39.0	35.0	40.0	31.0	41.0
60-61	36.71293027388157	38.5	35.0	40.0	31.0	41.0
62-63	36.50318589057859	38.0	35.0	40.0	30.0	41.0
64-65	36.30303152230759	38.0	34.5	40.0	30.0	41.0
66-67	36.05103124977782	37.5	34.0	40.0	30.0	41.0
68-69	35.77193582744239	37.0	34.0	39.5	29.5	41.0
70-71	35.47547332349262	36.5	34.0	39.0	29.0	41.0
72-73	35.16672459051972	36.0	34.0	39.0	29.0	40.0
74-75	34.82051945872436	35.5	33.5	38.5	29.0	40.0
76-77	33.76094044283316	35.0	32.0	37.0	27.5	39.0
78-79	34.144811384704155	35.0	33.0	37.0	29.0	39.0
80-81	34.01919832365907	35.0	33.0	37.0	29.0	39.0
82-83	33.76892018119649	35.0	33.0	36.0	29.0	38.5
84-85	33.522771955985675	35.0	33.0	36.0	29.0	37.0
86-87	33.29625681503262	35.0	33.0	35.5	29.0	37.0
88-89	33.0736993102812	34.5	32.0	35.0	29.0	36.5
90-91	32.93505383479594	34.0	32.0	35.0	29.0	36.0
92-93	32.843294445345904	34.0	32.0	35.0	28.5	36.0
94-95	32.78667558989345	34.0	32.0	35.0	29.0	36.0
96-97	32.77824546990231	34.0	32.0	35.0	29.0	35.0
98-99	32.87071341865664	34.0	32.0	35.0	29.0	35.0
100-101	32.24079712903807	33.5	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	4.0
19	8.0
20	47.0
21	343.0
22	1519.0
23	5234.0
24	13976.0
25	29983.0
26	54643.0
27	88734.0
28	130805.0
29	179686.0
30	236070.0
31	304090.0
32	387529.0
33	497929.0
34	655562.0
35	882436.0
36	1253799.0
37	1847235.0
38	2051885.0
39	946271.0
40	33033.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.09489834254799	22.35961903674696	15.378289002575926	24.167193618129115
2	13.81657260828172	22.960755883596757	41.2190300007708	22.00364150735072
3	16.713475158689537	27.926025500733164	31.885540633916555	23.474958706660743
4	11.991150341085378	19.798065207333288	39.92752912198559	28.283255329595736
5	12.32577922242275	38.582877443501964	36.618045477569055	12.47329785650623
6	28.037539706239706	39.99533998186197	19.438181380529855	12.528938931368474
7	24.572617279293095	33.44658753662838	24.95234522130972	17.02844996276881
8	23.259489995699326	37.7606040150108	22.165729368352977	16.814176620936898
9	24.8977222991948	16.78598978295817	22.171570309292264	36.144717608554764
10-11	23.141955970020067	27.514904452972882	30.21419415962508	19.12894541738197
12-13	24.09785849586629	25.14983092072741	30.084835444298413	20.66747513910788
14-15	21.362696860747967	26.533847830946144	27.566873961417055	24.536581346888838
16-17	20.91813076005367	29.519852570956946	28.24198802977495	21.320028639214435
18-19	20.971936402058894	28.77866465571947	29.797352455385802	20.45204648683584
20-21	21.703925686120105	27.99823131632564	29.61739455603748	20.680448441516777
22-23	21.404249483207494	27.971409122206175	29.466561583222955	21.15777981136338
24-25	21.255632346745934	28.11314573553606	29.46184550393294	21.169376413785066
26-27	21.271699378887813	28.197581068652795	29.368231465601152	21.16248808685824
28-29	21.242943445553262	28.277129493471808	29.398715118283924	21.081211942691006
30-31	21.483610433073487	28.119853089575724	29.333219149737772	21.063317327613014
32-33	21.270540743534138	28.321916080047522	29.24818769076595	21.15935548565239
34-35	21.39266883215007	28.351590494738353	29.230837754036937	21.02490291907464
36-37	21.35830354407594	28.307959267874374	29.204768069365716	21.128969118683973
38-39	21.26152680936662	28.34890798452234	29.23960552924263	21.14995967686841
40-41	21.491510167074377	28.276257431573438	29.09810627757336	21.134126123778824
42-43	21.374797465857604	28.409343404113574	28.903886598267132	21.31197253176169
44-45	21.46607935644098	28.468980236910234	28.7284692909696	21.336471115679185
46-47	21.516602892247406	28.460353650509713	28.52269583479795	21.50034762244493
48-49	21.45495883945227	28.56561237895288	28.516261516106876	21.46316726548797
50-51	21.491166531564605	28.62951920044795	28.377088559357304	21.50222570863014
52-53	21.59354780693978	28.522408002095467	28.34460436105182	21.539439829912922
54-55	21.583734746910046	28.461641276602112	28.359094387805566	21.595529588682272
56-57	21.591543063811777	28.570176127839108	28.29219573509598	21.54608507325313
58-59	21.601129798554012	28.59027054129933	28.29865974073443	21.509939919412233
60-61	21.67821040381889	28.542159803917443	28.281624035308507	21.49800575695516
62-63	21.609998991506206	28.598352234861146	28.27425764233304	21.517391131299608
64-65	21.60250714658002	28.57694980528685	28.234520098743065	21.586022949390067
66-67	21.662438689124418	28.547724134057024	28.14101955724915	21.648817619569403
68-69	21.596425518353243	28.548859976627934	28.204654166620717	21.65006033839811
70-71	21.624857629273183	28.374612137619398	28.271859863844924	21.728670369262492
72-73	21.654961336541962	28.417338252979874	28.205214555076196	21.722485855401967
74-75	21.52916517293374	28.468737381549015	28.21412293707226	21.787974508444986
76-77	21.63808250174659	28.416439870282893	28.1712912274116	21.77418640055891
78-79	21.66684767012872	28.386621016800824	28.15094658204689	21.79558473102357
80-81	21.653589112676602	28.37884842194745	28.16401677476274	21.80354569061321
82-83	21.601830013007778	28.38397461238533	28.268146613257052	21.746048761349837
84-85	21.720582129076856	28.31156036521935	28.241505326115522	21.726352179588268
86-87	21.62658251930676	28.31121582736888	28.27199511496462	21.79020653835974
88-89	21.63777408459536	28.331347612133722	28.224964853340712	21.805913449930205
90-91	21.701185787719663	28.288792204473324	28.2657241821621	21.744297825644914
92-93	21.67601201827278	28.18985137929196	28.325078763702532	21.80905783873273
94-95	21.509923215024674	28.229904093944697	28.389568737759436	21.870603953271196
96-97	21.457374576099813	28.286137510211752	28.476175367359485	21.780312546328947
98-99	21.61471007239894	28.893539855078643	28.552654036109054	20.93909603641336
100-101	22.125568727193013	31.64537747067567	25.814502640875347	20.41455116125597
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2280.0
1	2014.5
2	2692.5
3	3834.5
4	5159.5
5	7968.0
6	10310.5
7	12143.5
8	13273.5
9	13135.0
10	12329.5
11	11676.0
12	13122.5
13	17329.5
14	26786.5
15	39702.0
16	49741.5
17	54670.0
18	55852.0
19	54888.5
20	53096.0
21	52365.0
22	54787.0
23	62459.5
24	75297.5
25	93693.0
26	116428.0
27	139517.0
28	163591.5
29	187957.0
30	210120.0
31	232618.5
32	255823.5
33	278034.0
34	297959.5
35	315833.0
36	336362.5
37	355891.0
38	370761.5
39	381641.5
40	390020.0
41	396295.5
42	398653.5
43	399665.5
44	401433.5
45	401610.0
46	397508.0
47	392191.0
48	385033.0
49	374100.5
50	361157.0
51	344036.0
52	323556.5
53	301638.5
54	278062.5
55	254153.5
56	230758.0
57	206540.5
58	180945.5
59	155242.5
60	129107.0
61	104246.5
62	82409.5
63	63729.0
64	47834.5
65	35900.5
66	27254.5
67	20634.0
68	15710.5
69	11345.0
70	8007.0
71	5843.5
72	4140.0
73	2889.5
74	2001.0
75	1355.0
76	941.5
77	632.5
78	427.0
79	300.5
80	222.0
81	166.0
82	119.5
83	82.5
84	60.0
85	47.5
86	39.5
87	34.5
88	27.0
89	21.0
90	15.0
91	10.0
92	7.0
93	3.5
94	1.5
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0415774815948051E-5
2	0.004187141476011116
3	0.0
4	0.0
5	1.0415774815948051E-5
6	1.0415774815948051E-5
7	1.0415774815948051E-5
8	1.0415774815948051E-5
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	4.11423105229948E-4
20-21	5.624757432335731E-4
22-23	2.239728682392618E-4
24-25	1.979777097938531E-4
26-27	0.0026842125562772526
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.033306125413449884
38-39	0.016722968923397478
40-41	0.021807792054776547
42-43	0.010760345562045838
44-45	0.004317362562459344
46-47	0.005506314127339053
48-49	0.00966193857603642
50-51	0.007417259151662521
52-53	0.011575778722457755
54-55	0.004007906642265262
56-57	0.0175079663564323
58-59	0.014032649688722329
60-61	0.014293520825168392
62-63	0.0053803391038018825
64-65	0.007167843493494411
66-67	4.956429314892969E-4
68-69	5.699867527644047E-4
70-71	0.001261248643687093
72-73	1.1437281917244275E-4
74-75	0.003570676982985113
76-77	0.004451006764943767
78-79	0.0035803052560334416
80-81	5.763660948757569E-4
82-83	0.003734053500076619
84-85	0.004962273265003958
86-87	0.0018429260789035837
88-89	0.07481042197081368
90-91	0.0012908063406147864
92-93	0.0
94-95	5.590044282215652E-4
96-97	0.012125471024931023
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	263.0
20-21	813.0
22-23	1706.0
24-25	3762.0
26-27	5222.0
28-29	8856.0
30-31	21924.0
32-33	29312.0
34-35	42045.0
36-37	63031.0
38-39	70298.0
40-41	72671.0
42-43	84937.0
44-45	98585.0
46-47	100754.0
48-49	90878.0
50-51	83017.0
52-53	80972.0
54-55	88148.0
56-57	93204.0
58-59	92777.0
60-61	91371.0
62-63	87704.0
64-65	92538.0
66-67	100286.0
68-69	102024.0
70-71	100489.0
72-73	97300.0
74-75	98662.0
76-77	101561.0
78-79	107621.0
80-81	110300.0
82-83	106597.0
84-85	107854.0
86-87	114575.0
88-89	121396.0
90-91	132696.0
92-93	137709.0
94-95	161474.0
96-97	222496.0
98-99	784028.0
100-101	5488966.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.2849664659509
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.9495170794723	76.71341547751274
2	6.881529391076871	11.737820067048931
3	1.7683088837108094	4.524304915461584
4	0.6614970386416219	2.256630110315062
5	0.28102796409787184	1.1983730247040727
6	0.15472609008644517	0.7917485642658105
7	0.09434140968271194	0.5632132772798436
8	0.05790723834587118	0.39508935043707594
9	0.03667453527423439	0.28150078599156725
>10	0.11308471013303815	1.4385914933694428
>50	0.0011546732162423493	0.060517147232483144
>100	2.309862619648511E-4	0.038795786381443556
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0415774815948051E-5
2	0.0	0.0	0.0	1.0415774815948051E-5	1.0415774815948051E-5
3	0.0	0.0	0.0	1.0415774815948051E-5	1.0415774815948051E-5
4	0.0	3.1247324447844155E-5	0.0	3.1247324447844155E-5	2.0831549631896102E-5
5	0.0	3.1247324447844155E-5	0.0	3.1247324447844155E-5	2.0831549631896102E-5
6	0.0	3.1247324447844155E-5	0.0	3.1247324447844155E-5	3.1247324447844155E-5
7	0.0	3.1247324447844155E-5	0.0	3.1247324447844155E-5	3.1247324447844155E-5
8	0.0	4.1663099263792204E-5	0.0	3.1247324447844155E-5	3.1247324447844155E-5
9	0.0	8.332619852758441E-5	0.0	7.291042371163635E-5	3.1247324447844155E-5
10-11	1.0415774815948051E-5	8.332619852758441E-5	0.0	9.374197334353246E-5	3.1247324447844155E-5
12-13	1.0415774815948051E-5	8.332619852758441E-5	0.0	9.374197334353246E-5	3.645521185581818E-5
14-15	1.0415774815948051E-5	1.7186028446314284E-4	0.0	1.5623662223922076E-4	4.6870986671766225E-5
16-17	1.5623662223922077E-5	1.7706817187111687E-4	0.0	1.6665239705516882E-4	7.291042371163635E-5
18-19	2.0831549631896102E-5	1.9269183409503896E-4	0.0	1.6665239705516882E-4	7.291042371163635E-5
20-21	2.0831549631896102E-5	2.0831549631896101E-4	0.0	1.6665239705516882E-4	7.291042371163635E-5
22-23	2.0831549631896102E-5	2.447707081747792E-4	0.0	1.7186028446314284E-4	1.0936563556745454E-4
24-25	2.0831549631896102E-5	2.603943703987013E-4	0.0	2.0310760891098699E-4	1.458208474232727E-4
26-27	2.0831549631896102E-5	2.7081014521464935E-4	0.0	2.9164169484654546E-4	1.614445096471948E-4
28-29	2.0831549631896102E-5	2.7081014521464935E-4	0.0	9.530433956592467E-4	1.6665239705516882E-4
30-31	2.0831549631896102E-5	2.968495822545194E-4	0.0	0.0023383414461803373	1.6665239705516882E-4
32-33	2.0831549631896102E-5	3.0205746966249346E-4	0.0	0.005681805162099662	1.7706817187111687E-4
34-35	2.6039437039870127E-5	3.3330479411033763E-4	0.0	0.010139756783325429	1.7706817187111687E-4
36-37	3.1247324447844155E-5	3.645521185581818E-4	0.0	0.01598821434248026	1.7706817187111687E-4
38-39	3.645521185581818E-5	3.853836681900779E-4	0.0	0.023810461229257243	1.7706817187111687E-4
40-41	5.2078874079740254E-5	4.0621521782197397E-4	0.0	0.0325805436242855	1.874839466870649E-4
42-43	5.728676148771428E-5	4.270467674538701E-4	0.0	0.041043360662243296	1.9789972150301296E-4
44-45	6.249464889568831E-5	4.895414163495584E-4	0.0	0.050433181658820464	1.9789972150301296E-4
46-47	6.249464889568831E-5	5.312045156133506E-4	0.0	0.06096873788515192	2.0310760891098699E-4
48-49	6.249464889568831E-5	5.312045156133506E-4	0.0	0.07112411833070127	2.3956282076680518E-4
50-51	6.249464889568831E-5	5.624518400611948E-4	0.0	0.08067538383692563	2.3956282076680518E-4
52-53	6.249464889568831E-5	5.624518400611948E-4	0.0	0.08954962398011337	2.656022578066753E-4
54-55	6.249464889568831E-5	5.832833896930909E-4	0.0	0.09897590018854635	2.916416948465454E-4
56-57	6.249464889568831E-5	6.041149393249869E-4	0.0	0.10735539102797656	2.916416948465454E-4
58-59	6.249464889568831E-5	6.041149393249869E-4	0.0	0.11390691338720789	2.916416948465454E-4
60-61	6.249464889568831E-5	6.14530714140935E-4	0.0	0.12075007744128576	3.228890192943896E-4
62-63	6.249464889568831E-5	6.353622637728312E-4	0.0	0.12734326289978087	3.3330479411033763E-4
64-65	6.249464889568831E-5	7.395200119323116E-4	0.0	0.13407706131829128	3.3330479411033763E-4
66-67	6.249464889568831E-5	7.655594489721818E-4	0.0	0.14077440452494588	3.3330479411033763E-4
68-69	6.249464889568831E-5	7.863909986040778E-4	0.0	0.14815398098204507	3.3330479411033763E-4
70-71	6.249464889568831E-5	7.968067734200259E-4	0.0	0.15518462898281002	3.437205689262857E-4
72-73	6.249464889568831E-5	8.07222548235974E-4	0.0	0.1609237209063974	3.5413634374223375E-4
74-75	6.249464889568831E-5	8.332619852758441E-4	0.0	0.16620972662549102	3.5413634374223375E-4
76-77	6.249464889568831E-5	8.749250845396363E-4	0.0	0.17082391486895604	3.5413634374223375E-4
78-79	6.249464889568831E-5	8.853408593555844E-4	0.0	0.17505792733163889	3.5413634374223375E-4
80-81	6.249464889568831E-5	9.009645215795063E-4	0.0	0.17708900342074876	3.5934423115020775E-4
82-83	6.249464889568831E-5	9.061724089874804E-4	0.0	0.17826598597495089	3.957994430060259E-4
84-85	6.249464889568831E-5	9.165881838034285E-4	0.0	0.17945859219137694	3.957994430060259E-4
86-87	6.249464889568831E-5	9.270039586193765E-4	0.0	0.17985959952179095	3.957994430060259E-4
88-89	6.249464889568831E-5	0.001031161706778857	0.0	0.17988043107142285	3.957994430060259E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11230	0.0	45.467594	1
GTATCAA	19335	0.0	31.949503	1
TCAACGC	23195	0.0	26.018047	4
ATCAACG	23370	0.0	25.787401	3
CAACGCA	23700	0.0	25.393019	5
AACGCAG	24270	0.0	24.848377	6
TATCAAC	25265	0.0	24.019867	2
ACGCAGA	28180	0.0	21.445196	7
CGCAGAG	28325	0.0	21.32064	8
GTACATG	23525	0.0	18.929272	1
TACATGG	23475	0.0	18.399075	2
AGAGTAC	30640	0.0	17.578976	10-11
ACATGGG	23975	0.0	17.560753	3
GCAGAGT	34045	0.0	17.492641	9
GAGTACT	20515	0.0	17.268682	12-13
CAGAGTA	33555	0.0	16.432255	10-11
CATGGGG	17105	0.0	15.707814	4
AGTACTT	23155	0.0	15.335957	12-13
GTGGTAT	6245	0.0	15.280033	1
GTACTTT	23845	0.0	14.928372	14-15
>>END_MODULE