FastQCFastQC Report
Fri 27 May 2016
SRR2139827_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139827_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7288201
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75050.044.417421
GTATCAA133750.032.7313231
ATCAACG161600.026.4818693
TCAACGC161900.026.458094
CAACGCA167100.025.6102335
TATCAAC172700.024.9461212
AACGCAG172450.024.7919656
ACGCAGA200350.021.3599687
CGCAGAG202200.021.1240338
GTACATG174350.019.025791
TACATGG179300.018.0894172
AGAGTAC214450.017.53988810-11
ACATGGG182800.017.384383
GCAGAGT244600.017.3451069
GAGTACT138100.016.65059512-13
TATACCG4051.4551915E-1116.1785145
CAGAGTA236300.015.848701510-11
GTGGTAT40400.015.8133141
CATGGGG128400.015.3728964
AGTACTT157700.015.04857612-13
Produced by FastQC (version 0.11.5)