FastQCFastQC Report
Fri 27 May 2016
SRR2139826_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139826_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10171184
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111550.045.117091
GTATCAA194200.033.3016621
TCAACGC233100.026.884434
ATCAACG237000.026.4772133
CAACGCA239000.026.2207575
AACGCAG245200.025.6087676
TATCAAC253950.025.088722
ACGCAGA284800.022.0333567
CGCAGAG289150.021.7018818
GTACATG255550.018.4376641
TACATGG253150.017.871262
AGAGTAC312350.017.7539210-11
GCAGAGT349150.017.7456079
ACATGGG252300.017.5671023
GAGTACT206250.017.21380812-13
CAGAGTA340800.016.26570510-11
GTGGTAT55700.015.570821
CATGGGG180800.015.5664474
AGTACTT230250.015.4285912-13
GTACTTT235900.014.99775314-15
Produced by FastQC (version 0.11.5)