##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139826_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10171184
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.885667391328287	31.0	31.0	34.0	30.0	34.0
2	32.04481208874011	31.0	31.0	34.0	30.0	34.0
3	32.07618237955385	33.0	31.0	34.0	30.0	34.0
4	35.7908767553512	37.0	35.0	37.0	35.0	37.0
5	35.61500352368023	37.0	35.0	37.0	33.0	37.0
6	35.625194470968175	37.0	35.0	37.0	33.0	37.0
7	35.524293140306966	37.0	35.0	37.0	33.0	37.0
8	35.50790576593639	37.0	35.0	37.0	33.0	37.0
9	37.071688507454	39.0	37.0	39.0	33.0	39.0
10-11	36.95576685074225	39.0	37.0	39.0	33.0	39.0
12-13	36.9074866800168	39.0	37.0	39.0	33.0	39.0
14-15	38.04999953791024	40.0	37.0	41.0	33.0	41.0
16-17	38.031319559256815	40.0	37.0	41.0	33.0	41.0
18-19	38.050636828514754	40.0	37.0	41.0	33.0	41.0
20-21	38.02777981177861	40.0	37.0	41.0	33.0	41.0
22-23	37.96066986671279	40.0	37.0	41.0	33.0	41.0
24-25	37.87926713178986	40.0	37.0	41.0	33.0	41.0
26-27	37.70056606243844	39.0	37.0	41.0	32.0	41.0
28-29	37.59710965137046	39.0	37.0	41.0	32.0	41.0
30-31	37.44940061547342	39.0	36.0	40.5	32.0	41.0
32-33	37.630725777988474	39.0	37.0	41.0	32.0	41.0
34-35	37.587916122721346	39.0	36.5	41.0	32.0	41.0
36-37	37.50081520091864	39.0	36.0	41.0	32.0	41.0
38-39	37.39513807371536	39.0	36.0	41.0	31.0	41.0
40-41	37.303907967871844	39.0	36.0	41.0	31.0	41.0
42-43	37.25859714551633	39.0	36.0	40.0	31.0	41.0
44-45	37.28240803193199	39.0	36.0	40.5	31.5	41.0
46-47	37.389539789904305	39.0	36.0	41.0	31.5	41.0
48-49	37.336296014042276	39.0	36.0	41.0	31.0	41.0
50-51	37.22774106378927	39.0	36.0	41.0	31.0	41.0
52-53	37.13307976992384	39.0	36.0	40.5	31.0	41.0
54-55	37.007433968837745	39.0	35.0	40.0	31.0	41.0
56-57	36.88049404729829	39.0	35.0	40.0	31.0	41.0
58-59	36.74035814226458	38.5	35.0	40.0	31.0	41.0
60-61	36.517851309579584	38.0	35.0	40.0	30.0	41.0
62-63	36.3062231030309	38.0	34.5	40.0	30.0	41.0
64-65	36.10005047445777	38.0	34.0	40.0	30.0	41.0
66-67	35.84232450846098	37.0	34.0	40.0	29.0	41.0
68-69	35.560567164016334	37.0	34.0	39.5	29.0	41.0
70-71	35.259727807023026	36.0	34.0	39.0	29.0	40.5
72-73	34.95138174787909	36.0	33.5	39.0	29.0	40.0
74-75	34.60613349355085	35.0	33.0	38.0	29.0	40.0
76-77	33.555072337667866	34.5	31.5	37.0	27.5	39.0
78-79	33.95389202031349	35.0	33.0	37.0	28.5	39.0
80-81	33.84221203150693	35.0	33.0	36.5	29.0	39.0
82-83	33.602532967091186	35.0	33.0	36.0	29.0	38.5
84-85	33.364642737251785	35.0	32.5	36.0	28.5	37.0
86-87	33.14529111708704	35.0	32.0	35.0	28.0	37.0
88-89	32.92800908357608	34.0	32.0	35.0	28.0	36.5
90-91	32.797304083571234	34.0	32.0	35.0	27.5	36.0
92-93	32.71081052662994	34.0	32.0	35.0	27.5	36.0
94-95	32.66057930679692	34.0	32.0	35.0	28.0	35.5
96-97	32.66000807587734	34.0	32.0	35.0	28.5	35.0
98-99	32.76387623124904	34.0	32.0	35.0	29.0	35.0
100-101	32.135816806203884	33.5	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	2.0
19	11.0
20	81.0
21	419.0
22	1867.0
23	6314.0
24	16574.0
25	35796.0
26	65065.0
27	105044.0
28	153175.0
29	208091.0
30	272111.0
31	346253.0
32	434887.0
33	553123.0
34	720195.0
35	954889.0
36	1341447.0
37	1939352.0
38	2064107.0
39	921274.0
40	31106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.85355765857741	22.412769250856144	15.368436948933379	24.36523614163307
2	13.815555509017049	23.081385328119577	40.94890770027773	22.15415146258565
3	16.7514912718126	27.91501952968307	31.870910997185774	23.46257820131855
4	12.019269339734686	19.839853452656055	39.81276909354899	28.328108114060267
5	12.360391116746204	38.51494953930138	36.60956645849357	12.51509288545885
6	28.11230236322536	40.042958617207205	19.32967685964584	12.515062159921598
7	24.576430826538974	33.31229677882142	24.919350588879325	17.191921805760273
8	23.277752127972516	37.65509502138591	22.1446883666641	16.922464483977482
9	24.894132285877436	16.720491930929576	22.0264818727102	36.35889391048279
10-11	23.202141461603684	27.406170215778218	30.184892928886153	19.20679539373194
12-13	24.119807487505877	25.040447601773796	30.011874723729314	20.827870186991014
14-15	21.379393982057547	26.436528923279727	27.414320692654858	24.76975640200787
16-17	21.03338706683509	29.43074768876465	28.10394050486158	21.43192473953868
18-19	21.065802483270442	28.699914616286755	29.619432662610652	20.614850237832158
20-21	21.832244224636607	27.898275851558722	29.42009804239668	20.84938188140799
22-23	21.549173094808197	27.905092881671127	29.25743632545843	21.288297698062244
24-25	21.385794191729556	28.039854538988067	29.263445519445376	21.310905749837
26-27	21.41920077277881	28.13497002722034	29.16316554155893	21.28266365844192
28-29	21.365716262423202	28.141353028277898	29.23923732876525	21.253693380533647
30-31	21.581038464929964	28.062211110822492	29.102689026315844	21.2540613979317
32-33	21.457980595215894	28.196858578931955	29.023477596861603	21.321683228990544
34-35	21.550292712502575	28.200262461817278	29.03730633599525	21.212138489684897
36-37	21.498056326799066	28.14691691274299	29.01215228126537	21.342874479192574
38-39	21.36653533290446	28.216101567096086	29.082674518819175	21.33468858118028
40-41	21.585854798468166	28.15859084135701	28.943182103845473	21.31237225632935
42-43	21.47107568415125	28.285011951218852	28.736767054933175	21.50714530969672
44-45	21.58514938278623	28.328425035900807	28.569854361773032	21.516571219539927
46-47	21.63253194444025	28.29195605555725	28.394126395422703	21.6813856045798
48-49	21.551573699529563	28.389875892513267	28.387132411755495	21.671417996201676
50-51	21.57298631899692	28.507687162130424	28.21864154571762	21.700684973155035
52-53	21.725728510212342	28.39455695640922	28.173822328794095	21.70589220458434
54-55	21.678204586492633	28.324415243614364	28.225637342830534	21.771742827062464
56-57	21.721169916419267	28.39728679970778	28.15555339506736	21.725989888805593
58-59	21.705958897253673	28.437594880165744	28.143655260204913	21.71279096237567
60-61	21.76074847637989	28.417751889428054	28.127983303764655	21.6935163304274
62-63	21.7270241439363	28.48701092507417	28.122210263631896	21.663754667357633
64-65	21.72875553950423	28.428154058448335	28.08060566622964	21.762484735817793
66-67	21.774620502350878	28.46270250687759	27.973600264508256	21.789076726263275
68-69	21.73596855295902	28.418747068131335	28.055207116377485	21.790077262532165
70-71	21.75695673761059	28.229088575697602	28.097509855739588	21.916444830952216
72-73	21.79586286409548	28.25581464865904	28.056622973729706	21.891699513515775
74-75	21.654010943037772	28.36720515060061	28.071789019944966	21.906994886416655
76-77	21.800006969858835	28.303809201478654	27.98352434508441	21.912659483578107
78-79	21.74049301618786	28.280076001908665	28.017361119191058	21.962069862712415
80-81	21.77607107563868	28.268614278931114	28.005412559866755	21.94990208556345
82-83	21.773925099974676	28.251058436183037	28.112927528938584	21.862088934903703
84-85	21.819021626408645	28.228293755699607	28.078215895360977	21.874468722530775
86-87	21.72502410132476	28.223602770302886	28.123699242489657	21.927673885882694
88-89	21.746502051112277	28.176037359754186	28.101531930227186	21.975928658906355
90-91	21.782841813763433	28.15551459847993	28.184062539818417	21.877581047938218
92-93	21.800383630274997	28.103807853461188	28.172019639843747	21.923788876420065
94-95	21.63772170294952	28.097894391710103	28.260884349115244	22.003499556225133
96-97	21.572888436516543	28.15429876390752	28.3325523738034	21.940260425772543
98-99	21.742981024845164	28.785212895511446	28.39233396846809	21.079472111175296
100-101	22.242004066936182	31.478561010134147	25.67489617694933	20.60453874598034
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2355.0
1	2098.5
2	2788.0
3	3904.0
4	5097.0
5	7646.5
6	9771.0
7	11626.0
8	12824.0
9	12832.5
10	12122.0
11	11444.5
12	12832.0
13	16959.0
14	26576.0
15	39482.0
16	49418.0
17	54574.0
18	55894.0
19	54947.5
20	53279.5
21	52597.5
22	54881.0
23	63084.0
24	77200.0
25	97028.5
26	122572.5
27	149027.0
28	175286.0
29	201517.5
30	224420.5
31	245765.0
32	268233.0
33	291683.5
34	314223.5
35	334887.0
36	357341.0
37	376127.0
38	387387.5
39	396922.0
40	406326.5
41	413943.5
42	416648.5
43	418414.0
44	420771.0
45	421416.0
46	419766.5
47	416299.5
48	410527.5
49	398835.5
50	385654.5
51	370836.5
52	350296.0
53	328267.0
54	305002.0
55	280464.0
56	256425.5
57	230780.0
58	203340.0
59	174930.0
60	146388.0
61	119155.5
62	95093.0
63	73877.0
64	55107.0
65	40983.0
66	30835.5
67	23322.0
68	17759.0
69	12919.0
70	9205.5
71	6628.5
72	4637.0
73	3199.0
74	2225.5
75	1525.0
76	1014.5
77	672.5
78	460.5
79	328.0
80	236.5
81	168.0
82	133.5
83	100.0
84	69.5
85	51.5
86	38.0
87	28.5
88	20.0
89	15.0
90	12.5
91	11.5
92	11.0
93	7.0
94	2.5
95	1.5
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0038835203453206632
3	0.0
4	0.0
5	9.831697076761171E-6
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	3.981837316088274E-4
20-21	5.850102767624626E-4
22-23	2.5566313510656043E-4
24-25	1.4753414382690587E-4
26-27	0.00218930836255773
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.032496133548047645
38-39	0.01686163010110103
40-41	0.021931858418804538
42-43	0.010595891415058862
44-45	0.004299467211053019
46-47	0.005493847125554091
48-49	0.009582833964133033
50-51	0.007388782073378426
52-53	0.011547016659068909
54-55	0.003753321716838779
56-57	0.01695750459217777
58-59	0.013462679296079305
60-61	0.013478270453527817
62-63	0.004968738354519482
64-65	0.006498757808045279
66-67	3.309245125191646E-4
68-69	6.11659932133978E-4
70-71	9.833442931524551E-4
72-73	9.058276239018199E-5
74-75	0.00305918451318432
76-77	0.0039870467485881795
78-79	0.0031445300867695342
80-81	5.363746285605697E-4
82-83	0.0034434265764830435
84-85	0.004812952913847945
86-87	0.0016186570965238001
88-89	0.07258700268071122
90-91	9.844020797227036E-4
92-93	7.075177010316032E-6
94-95	3.834362208468196E-4
96-97	0.011651683805869712
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	253.0
20-21	908.0
22-23	1881.0
24-25	3896.0
26-27	5454.0
28-29	9322.0
30-31	23390.0
32-33	31191.0
34-35	44622.0
36-37	67641.0
38-39	76304.0
40-41	79100.0
42-43	91188.0
44-45	106661.0
46-47	108022.0
48-49	98743.0
50-51	91022.0
52-53	89142.0
54-55	97223.0
56-57	102130.0
58-59	103052.0
60-61	100775.0
62-63	97318.0
64-65	102278.0
66-67	110414.0
68-69	111677.0
70-71	111016.0
72-73	107725.0
74-75	108091.0
76-77	111099.0
78-79	118881.0
80-81	121136.0
82-83	116595.0
84-85	117327.0
86-87	124895.0
88-89	132964.0
90-91	144454.0
92-93	150425.0
94-95	178041.0
96-97	240913.0
98-99	829121.0
100-101	5704894.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.47712447181516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.49402289480068	78.25662881828968
2	6.755945573179364	11.684694925132808
3	1.5706483119270709	4.074754487158909
4	0.5752284317920989	1.9897640278324948
5	0.24073011293057395	1.0408823970005678
6	0.12605474498660754	0.6540511123481868
7	0.07670213694810489	0.46430861708808346
8	0.04381612412285688	0.3031273935715838
9	0.02620522613907223	0.20395373423765523
>10	0.08868283464131897	1.178740381544063
>50	0.0016686019454592708	0.09186346861133074
>100	2.6010614053988606E-4	0.03728107489403751
>500	3.490044626531811E-5	0.019949562290552836
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.899018246056703E-5	0.0	0.0	9.831697076761171E-6
3	0.0	5.899018246056703E-5	0.0	0.0	9.831697076761171E-6
4	0.0	1.0814866784437288E-4	0.0	9.831697076761171E-6	9.831697076761171E-6
5	0.0	1.1798036492113406E-4	0.0	9.831697076761171E-6	1.9663394153522342E-5
6	1.0814866784437288E-4	1.1798036492113406E-4	0.0	9.831697076761171E-6	2.9495091230283515E-5
7	1.0814866784437288E-4	1.1798036492113406E-4	0.0	9.831697076761171E-6	2.9495091230283515E-5
8	1.0814866784437288E-4	1.2781206199789522E-4	0.0	9.831697076761171E-6	2.9495091230283515E-5
9	1.0814866784437288E-4	2.2612903276550696E-4	0.0	9.831697076761171E-6	2.9495091230283515E-5
10-11	1.0814866784437288E-4	2.2612903276550696E-4	0.0	4.915848538380586E-5	3.9326788307044685E-5
12-13	1.0814866784437288E-4	2.2612903276550696E-4	0.0	4.915848538380586E-5	3.9326788307044685E-5
14-15	1.1798036492113406E-4	2.8020336668769337E-4	0.0	4.915848538380586E-5	3.9326788307044685E-5
16-17	1.9663394153522344E-4	3.0478260937959634E-4	0.0	4.915848538380586E-5	4.915848538380586E-5
18-19	2.01549790073604E-4	3.391935491482604E-4	0.0	7.865357661408938E-5	5.899018246056703E-5
20-21	2.113814871503652E-4	3.785203374553051E-4	0.0	9.831697076761171E-5	6.88218795373282E-5
22-23	2.2121318422712636E-4	4.3751051991587213E-4	0.0	1.4747545615141758E-4	7.373772807570879E-5
24-25	2.2612903276550696E-4	4.6700561114615565E-4	0.0	2.6545582107255164E-4	8.848527369085055E-5
26-27	2.2612903276550696E-4	5.014165509148197E-4	0.0	7.373772807570879E-4	8.848527369085055E-5
28-29	2.2612903276550696E-4	5.112482479915809E-4	0.0	0.0034804207651734547	9.340112222923114E-5
30-31	2.3596072984226812E-4	5.849859760672897E-4	0.0	0.008548660608243839	9.831697076761172E-5
32-33	2.457924269190293E-4	5.899018246056703E-4	0.0	0.02057774198166113	9.831697076761172E-5
34-35	2.5562412399579045E-4	6.587237041429985E-4	0.0	0.035403941173416976	1.0814866784437288E-4
36-37	2.5562412399579045E-4	7.324614322187072E-4	0.0	0.053420526066581826	1.1306451638275347E-4
38-39	2.5562412399579045E-4	7.570406749106102E-4	0.0	0.07628413761858993	1.1798036492113406E-4
40-41	2.5562412399579045E-4	8.111150088327966E-4	0.0	0.09659642377917851	1.1798036492113406E-4
42-43	3.0478260937959634E-4	8.504417971398413E-4	0.0	0.11459826112672822	1.1798036492113406E-4
44-45	3.0478260937959634E-4	9.290953737539307E-4	0.0	0.13231497925905183	1.1798036492113406E-4
46-47	3.0478260937959634E-4	9.880855562144977E-4	0.0	0.14902886428954584	1.1798036492113406E-4
48-49	3.0478260937959634E-4	0.0010028331018296396	0.0	0.16117101017934587	1.2781206199789522E-4
50-51	3.2444600353311867E-4	0.0010962342240588706	0.0	0.17171550529417223	1.3272791053627582E-4
52-53	3.2444600353311867E-4	0.0011011500725972513	0.0	0.18070659227087033	1.5730715322817877E-4
54-55	3.2936185207149926E-4	0.0011011500725972513	0.0	0.19027774937509734	1.8188639592008169E-4
56-57	3.44109397686641E-4	0.0011404768609042958	0.0	0.19857570170788374	1.8680224445846225E-4
58-59	3.539410947634022E-4	0.0011404768609042958	0.0	0.20609694997160605	1.9663394153522342E-4
60-61	3.588569433017828E-4	0.0011650561035961987	0.0	0.21369685181194242	2.064656386119846E-4
62-63	3.736044889169245E-4	0.0011896353462881018	0.0	0.22083466388967105	2.2612903276550696E-4
64-65	3.736044889169245E-4	0.0013076157112092359	0.0	0.2277414310860958	2.2612903276550696E-4
66-67	4.03099580147208E-4	0.001317447408285997	0.0	0.2350758771053596	2.2612903276550696E-4
68-69	4.227629743007304E-4	0.001376437590746564	0.0	0.24266103140008083	2.2612903276550696E-4
70-71	4.5717391406939446E-4	0.0014010168334384668	0.0	0.2499954774193447	2.2612903276550696E-4
72-73	5.063323994532003E-4	0.0014108485305152282	0.0	0.2558060103917106	2.457924269190293E-4
74-75	5.161640965299615E-4	0.0014206802275919892	0.0	0.2609479879628567	2.457924269190293E-4
76-77	5.210799450683421E-4	0.001460007015899034	0.0	0.26553939049770414	2.457924269190293E-4
78-79	5.210799450683421E-4	0.0014747545615141756	0.0	0.2694819010254853	2.457924269190293E-4
80-81	5.210799450683421E-4	0.0014845862585909368	0.0	0.27163504268529604	2.507082754574099E-4
82-83	5.210799450683421E-4	0.001494417955667698	0.0	0.272952490093582	2.6053997253417104E-4
84-85	5.358274906834839E-4	0.0015091655012828397	0.0	0.27416178883402365	2.6545582107255164E-4
86-87	5.407433392218645E-4	0.0015534081381282652	0.0	0.2747369431130142	2.6545582107255164E-4
88-89	5.407433392218645E-4	0.0016222300176655933	0.0	0.2748057649925515	2.752875181493128E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11155	0.0	45.11709	1
GTATCAA	19420	0.0	33.301662	1
TCAACGC	23310	0.0	26.88443	4
ATCAACG	23700	0.0	26.477213	3
CAACGCA	23900	0.0	26.220757	5
AACGCAG	24520	0.0	25.608767	6
TATCAAC	25395	0.0	25.08872	2
ACGCAGA	28480	0.0	22.033356	7
CGCAGAG	28915	0.0	21.701881	8
GTACATG	25555	0.0	18.437664	1
TACATGG	25315	0.0	17.87126	2
AGAGTAC	31235	0.0	17.75392	10-11
GCAGAGT	34915	0.0	17.745607	9
ACATGGG	25230	0.0	17.567102	3
GAGTACT	20625	0.0	17.213808	12-13
CAGAGTA	34080	0.0	16.265705	10-11
GTGGTAT	5570	0.0	15.57082	1
CATGGGG	18080	0.0	15.566447	4
AGTACTT	23025	0.0	15.42859	12-13
GTACTTT	23590	0.0	14.997753	14-15
>>END_MODULE