FastQCFastQC Report
Fri 27 May 2016
SRR2139825_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139825_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14225902
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158500.046.160691
GTATCAA275950.033.1345831
TCAACGC328250.026.9976884
ATCAACG329400.026.9161833
CAACGCA338750.026.1856565
AACGCAG347150.025.5762396
TATCAAC355550.025.2793872
ACGCAGA399750.022.1583257
CGCAGAG404800.021.8922718
GTACATG356600.018.3737031
TACATGG348350.018.1457182
GCAGAGT490000.017.9314129
AGAGTAC433550.017.79585310-11
GAGTACT288600.017.55819512-13
ACATGGG350350.017.4545293
CAGAGTA481950.016.47928610-11
AGTACTT321100.015.6371712-13
GTACTTT327750.015.39778214-15
CATGGGG254500.015.2157024
TATACTG87950.014.3263065
Produced by FastQC (version 0.11.5)