##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139825_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14225902
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.023712380417074	33.0	31.0	34.0	30.0	34.0
2	32.17829618114901	33.0	31.0	34.0	30.0	34.0
3	32.23875540545689	34.0	31.0	34.0	30.0	34.0
4	35.89471929442506	37.0	35.0	37.0	35.0	37.0
5	35.77670892151514	37.0	35.0	37.0	35.0	37.0
6	35.79595473102514	37.0	35.0	37.0	33.0	37.0
7	35.68352171974754	37.0	35.0	37.0	33.0	37.0
8	35.6713124412076	37.0	35.0	37.0	33.0	37.0
9	37.2570856315473	39.0	37.0	39.0	33.0	39.0
10-11	37.17751816369886	39.0	37.0	39.0	33.0	39.0
12-13	37.14380444909574	39.0	37.0	39.0	33.0	39.0
14-15	38.34093788921082	40.0	38.0	41.0	33.0	41.0
16-17	38.34471831733411	40.0	38.0	41.0	33.0	41.0
18-19	38.38810365065076	40.0	38.0	41.0	33.5	41.0
20-21	38.36756555958364	40.0	38.0	41.0	34.0	41.0
22-23	38.28756258544118	40.0	38.0	41.0	33.5	41.0
24-25	38.21975188614525	40.0	38.0	41.0	33.0	41.0
26-27	38.06075715101098	40.0	37.0	41.0	33.0	41.0
28-29	37.96350209478629	40.0	37.0	41.0	33.0	41.0
30-31	37.844844407485944	40.0	37.0	41.0	33.0	41.0
32-33	38.00342404900327	40.0	37.0	41.0	33.0	41.0
34-35	37.98810099310576	40.0	37.0	41.0	33.0	41.0
36-37	37.91449390458016	40.0	37.0	41.0	33.0	41.0
38-39	37.73630221896242	40.0	37.0	41.0	33.0	41.0
40-41	37.76277842853773	40.0	37.0	41.0	33.0	41.0
42-43	37.73894333161359	40.0	37.0	41.0	33.0	41.0
44-45	37.722773290899	40.0	37.0	41.0	33.0	41.0
46-47	37.751958984199504	40.0	37.0	41.0	33.0	41.0
48-49	37.68340839742723	40.0	37.0	41.0	32.0	41.0
50-51	37.60657886025752	40.0	37.0	41.0	32.0	41.0
52-53	37.47898941886652	39.5	36.0	41.0	32.0	41.0
54-55	37.392072192026106	39.0	36.0	41.0	31.5	41.0
56-57	37.25989528535536	39.0	36.0	41.0	31.0	41.0
58-59	37.1280858301193	39.0	35.5	40.5	31.0	41.0
60-61	36.93816158877229	39.0	35.0	40.0	31.0	41.0
62-63	36.717170087886515	38.5	35.0	40.0	31.0	41.0
64-65	36.53073765942878	38.0	35.0	40.0	31.0	41.0
66-67	36.25436648957286	38.0	35.0	40.0	30.0	41.0
68-69	35.98537756229979	37.0	34.0	40.0	30.0	41.0
70-71	35.70473386124726	37.0	34.0	39.0	30.0	41.0
72-73	35.407893577968146	36.0	34.0	39.0	30.0	40.5
74-75	35.07225399177931	36.0	34.0	39.0	29.0	40.0
76-77	33.99730559100344	35.0	32.5	37.0	28.5	39.0
78-79	34.32089514222771	35.0	33.0	37.0	29.0	39.0
80-81	34.204978683099	35.0	33.0	37.0	29.0	39.0
82-83	33.976287826240124	35.0	33.0	36.0	29.0	38.5
84-85	33.7453090644815	35.0	33.0	36.0	29.0	37.0
86-87	33.52824753489742	35.0	33.0	35.5	29.0	37.0
88-89	33.301571070019385	35.0	33.0	35.0	29.0	36.5
90-91	33.186950065817	35.0	33.0	35.0	29.0	36.0
92-93	33.10800064142948	35.0	33.0	35.0	29.0	36.0
94-95	33.05425279005671	34.0	33.0	35.0	29.0	36.0
96-97	32.97353749148769	34.0	32.5	35.0	29.0	35.0
98-99	33.09176155876544	34.0	33.0	35.0	29.0	35.0
100-101	32.63334244664651	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	0.0
18	4.0
19	17.0
20	76.0
21	598.0
22	2477.0
23	8553.0
24	22658.0
25	47992.0
26	86248.0
27	136111.0
28	195088.0
29	259473.0
30	329945.0
31	409754.0
32	510286.0
33	643022.0
34	843296.0
35	1139346.0
36	1693053.0
37	2778588.0
38	3420811.0
39	1636081.0
40	62423.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.53018262040607	22.583552171243692	15.558837675108405	24.32742753324183
2	13.601435324731817	22.998094073999248	41.442829495577385	21.95764110569155
3	16.687881505075396	27.882321895878302	31.903180482461135	23.526616116585167
4	11.888567768848683	19.969826869326106	39.96836193585475	28.17324342597046
5	12.392296811829578	38.494873646676325	36.61563252720285	12.497197014291256
6	28.066684277735078	40.06329440481173	19.37579775257836	12.494223564874831
7	24.61500859488558	33.32236507744816	24.826833476007355	17.235792851658896
8	23.06402785566778	38.01574761305118	22.0984792387857	16.821745292495336
9	24.788157545300116	16.879991159787266	22.106570114148123	36.2252811807645
10-11	23.133443489207224	27.560607404718517	30.14128383564009	19.16466527043417
12-13	24.058003492502618	25.111156396269287	30.00422398523482	20.826616125993276
14-15	21.277708787815353	26.55108969540209	27.469534093514774	24.701667423267786
16-17	20.922328861818393	29.585638928202933	28.175366314206297	21.316665895772374
18-19	20.95867102135246	28.848525738473384	29.69625054355077	20.496552696623386
20-21	21.63895762035241	28.07378323341275	29.510607961063045	20.77665118517179
22-23	21.36037223870792	28.087777113475354	29.37064672559523	21.1812039222215
24-25	21.252175570183983	28.190523339341127	29.379036891341386	21.178264199133498
26-27	21.275925426079624	28.33966358414391	29.252801281530612	21.131609708245858
28-29	21.250152637881715	28.37434937553071	29.26837628132244	21.107121705265136
30-31	21.43476944375094	28.265029987994655	29.257438723026464	21.042761845227943
32-33	21.296769111605915	28.36746451556913	29.16870779829017	21.16705857453478
34-35	21.436723339484452	28.362908046910697	29.195276324819442	21.00509228878541
36-37	21.304156350759737	28.4303371611824	29.14201935755901	21.12348713049885
38-39	21.248222395539194	28.408587393758673	29.18010191186211	21.163088298840023
40-41	21.430492194118813	28.2975153721534	29.097994091271772	21.17399834245602
42-43	21.379097222697823	28.393141471092797	28.87473995168351	21.353021354525872
44-45	21.38442779813887	28.52275768549426	28.728733101708897	21.36408141465797
46-47	21.485794049791654	28.448317684676645	28.495073501281276	21.57081476425042
48-49	21.442834951100313	28.519757114795958	28.504053070952075	21.533354863151654
50-51	21.437679941499145	28.6167359308187	28.40183420640004	21.543749921282114
52-53	21.55357116882063	28.545223270303026	28.344388327335	21.55681723354134
54-55	21.496275855208708	28.49982204008367	28.352352988446487	21.651549116261133
56-57	21.571865371160033	28.533702502109698	28.285420590245003	21.60901153648527
58-59	21.52558742440917	28.59732055175474	28.351185501184368	21.52590652265172
60-61	21.60488268356503	28.601832280290907	28.267435274974616	21.525849761169454
62-63	21.541921114305868	28.68074277939411	28.23347196289466	21.543864143405365
64-65	21.500552142948916	28.613446266489067	28.248499293354605	21.63750229720741
66-67	21.572713932861788	28.624213349112253	28.114221813799347	21.688850904226616
68-69	21.568497447995263	28.598403724213156	28.125547263469215	21.707551564322365
70-71	21.555764345405873	28.447140666849148	28.192355491872966	21.804739495872017
72-73	21.572262817652216	28.43143569528095	28.171536731056644	21.82476475601019
74-75	21.54127579652228	28.45525722657314	28.17555319450708	21.827913782397495
76-77	21.653212602934254	28.44158522957069	28.079634641510903	21.82556752598415
78-79	21.6440887791152	28.413466175096126	28.07169540780074	21.870749637987934
80-81	21.654910443278556	28.42593597244345	28.053818623378856	21.865334960899137
82-83	21.65427047901123	28.368220590550248	28.161586310841397	21.81592261959712
84-85	21.76840528760303	28.34207132766383	28.12678593536575	21.76273744936739
86-87	21.649296015029954	28.37277306007295	28.1605525565491	21.81737836834799
88-89	21.688727182557788	28.33270301472725	28.191995126827933	21.786574675887028
90-91	21.747384135906255	28.318440552154282	28.199337054469943	21.734838257469523
92-93	21.75987904660193	28.23183708235648	28.253822150469237	21.754461720572348
94-95	21.644065837658623	28.291304033536186	28.28759908963085	21.777031039174346
96-97	21.558072180843705	28.307349416279383	28.403514849958523	21.731063552918393
98-99	21.754791800798266	28.892798252056323	28.47041447877135	20.88199546837406
100-101	22.29252162188169	31.59749863378636	25.74298722385993	20.366992520472024
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3027.0
1	2696.0
2	3698.5
3	5334.0
4	7253.5
5	11257.0
6	14490.0
7	16743.5
8	18005.0
9	17747.5
10	16715.5
11	15895.0
12	17832.5
13	23744.0
14	37419.5
15	56022.5
16	70482.0
17	77832.0
18	79699.0
19	78402.5
20	76145.5
21	75004.0
22	77803.5
23	88743.0
24	107143.5
25	132468.0
26	164948.5
27	199229.0
28	234947.0
29	270645.0
30	302857.5
31	336901.0
32	372126.0
33	405303.0
34	437718.5
35	468320.0
36	500344.5
37	528970.5
38	550034.5
39	566962.5
40	580830.5
41	592472.5
42	600596.5
43	606467.5
44	609883.5
45	610015.0
46	607601.0
47	602817.5
48	590331.5
49	569905.0
50	547879.0
51	520732.0
52	487089.0
53	449273.0
54	408675.0
55	369290.0
56	332753.0
57	295624.0
58	257294.0
59	218568.0
60	178936.0
61	142482.0
62	111483.5
63	85796.0
64	64320.0
65	47935.0
66	36344.0
67	27301.5
68	20469.5
69	14855.0
70	10537.5
71	7621.0
72	5342.0
73	3686.0
74	2496.0
75	1641.0
76	1092.5
77	719.0
78	483.5
79	338.0
80	251.0
81	194.0
82	153.0
83	111.0
84	76.5
85	51.0
86	35.0
87	28.0
88	21.0
89	15.0
90	10.5
91	7.5
92	6.0
93	4.0
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0019049758672595944
3	7.029431244500349E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.00222489913087637
22-23	4.780704850250045E-4
24-25	0.0011919345480962994
26-27	0.011712737411999267
28-29	3.34306634843021E-4
30-31	0.0024979682428259075
32-33	0.002659625757807909
34-35	0.0017117656671479103
36-37	0.06394921274975275
38-39	0.0281667381926978
40-41	0.02612477661671675
42-43	0.0036099821872332015
44-45	0.0017246140036230133
46-47	0.003594756250619417
48-49	0.0030167384654178273
50-51	7.73895150864031E-4
52-53	0.0010067292770615816
54-55	0.0015754909826503304
56-57	0.012114124960532593
58-59	0.009323890324106228
60-61	0.004860694525174038
62-63	0.002751543136995712
64-65	0.001362042478242388
66-67	6.129322282892E-4
68-69	0.0017283075537868051
70-71	4.216271451651298E-5
72-73	6.402462643231092E-4
74-75	2.462519587831248E-4
76-77	0.005476840075001213
78-79	0.0015068835326172033
80-81	6.921811220453755E-4
82-83	4.923530056167904E-4
84-85	0.00301874929934274
86-87	0.003784734790454664
88-89	0.04364660632632887
90-91	4.3595526517705596E-4
92-93	5.772527201060922E-4
94-95	2.5169392528657115E-4
96-97	0.01876260782172992
98-99	1.8872752086719757E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	318.0
20-21	1226.0
22-23	2501.0
24-25	5066.0
26-27	6313.0
28-29	11401.0
30-31	33102.0
32-33	43699.0
34-35	62192.0
36-37	97098.0
38-39	101741.0
40-41	106820.0
42-43	122516.0
44-45	144650.0
46-47	144583.0
48-49	131668.0
50-51	121384.0
52-53	111483.0
54-55	122003.0
56-57	129955.0
58-59	132860.0
60-61	132345.0
62-63	129445.0
64-65	138915.0
66-67	150087.0
68-69	146847.0
70-71	146184.0
72-73	140750.0
74-75	143131.0
76-77	145345.0
78-79	156490.0
80-81	157497.0
82-83	152779.0
84-85	152936.0
86-87	160162.0
88-89	172542.0
90-91	186444.0
92-93	194350.0
94-95	239173.0
96-97	307875.0
98-99	1139520.0
100-101	8300506.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.23423547716592
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.64278329362986	75.50991316784173
2	7.007747879189672	11.805845700405458
3	1.7910575494777	4.52605090127581
4	0.6886406825535464	2.3202848565348675
5	0.3375785211815188	1.421783432261875
6	0.16806552658419224	0.8494122685132043
7	0.10331785927800755	0.6092030621194265
8	0.0635552236178486	0.42828205376238365
9	0.041833299492950046	0.3171416400248364
>10	0.1520343708108964	1.9492833367231917
>50	0.0027393760769147857	0.15490048455938207
>100	6.178328043839875E-4	0.08958745827653262
>500	2.8585302448431637E-5	0.018311637701257367
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.0294312445003485E-6	0.0	7.0294312445003485E-6	0.0
3	0.0	7.0294312445003485E-6	0.0	1.4058862489000697E-5	0.0
4	0.0	2.1088293733501046E-5	0.0	2.1088293733501046E-5	0.0
5	0.0	2.1088293733501046E-5	0.0	2.8117724978001394E-5	0.0
6	3.5147156222501746E-5	2.1088293733501046E-5	0.0	2.8117724978001394E-5	0.0
7	3.5147156222501746E-5	2.1088293733501046E-5	0.0	2.8117724978001394E-5	0.0
8	3.5147156222501746E-5	2.1088293733501046E-5	0.0	2.8117724978001394E-5	7.0294312445003485E-6
9	3.5147156222501746E-5	4.217658746700209E-5	0.0	3.5147156222501746E-5	1.4058862489000697E-5
10-11	3.5147156222501746E-5	4.217658746700209E-5	0.0	4.569130308925227E-5	1.4058862489000697E-5
12-13	3.5147156222501746E-5	4.217658746700209E-5	0.0	6.677959682275332E-5	1.4058862489000697E-5
14-15	3.5147156222501746E-5	5.2720734333752616E-5	0.0	8.435317493400418E-5	3.163244060025157E-5
16-17	7.732374368950384E-5	5.623544995600279E-5	0.0	9.138260617850453E-5	3.866187184475192E-5
18-19	7.732374368950384E-5	5.623544995600279E-5	0.0	1.1247089991200558E-4	4.217658746700209E-5
20-21	7.732374368950384E-5	6.326488120050313E-5	0.0	1.651916342457582E-4	4.569130308925227E-5
22-23	7.732374368950384E-5	6.677959682275332E-5	0.0	3.1983912162476585E-4	7.029431244500348E-5
24-25	7.732374368950384E-5	7.029431244500349E-5	0.0	0.00100520866796355	9.841203742300489E-5
26-27	7.732374368950384E-5	7.029431244500349E-5	0.0	0.0032616560974481623	9.841203742300489E-5
28-29	7.732374368950384E-5	7.029431244500349E-5	0.0	0.017387298183271612	9.841203742300489E-5
30-31	7.732374368950384E-5	7.732374368950384E-5	0.0	0.026149484229541296	9.841203742300489E-5
32-33	7.732374368950384E-5	7.732374368950384E-5	0.0	0.03332653353017616	9.841203742300489E-5
34-35	7.732374368950384E-5	8.435317493400418E-5	0.0	0.0432977817504999	9.841203742300489E-5
36-37	7.732374368950384E-5	9.138260617850453E-5	0.0	0.05585586066879977	9.841203742300489E-5
38-39	7.732374368950384E-5	9.138260617850453E-5	0.0	0.0785679530197804	9.841203742300489E-5
40-41	7.732374368950384E-5	9.138260617850453E-5	0.0	0.12041767193391323	9.841203742300489E-5
42-43	7.732374368950384E-5	9.841203742300489E-5	0.0	0.13882775236325962	1.0192675304525506E-4
44-45	7.732374368950384E-5	1.2652976240100627E-4	0.0	0.1658840332233415	1.089561842897554E-4
46-47	7.732374368950384E-5	1.4761805613450733E-4	0.0	0.19594891065606945	1.1247089991200558E-4
48-49	7.732374368950384E-5	1.5113277175675752E-4	0.0	0.22097720060211296	1.3355919364550664E-4
50-51	9.138260617850453E-5	2.1088293733501046E-4	0.0	0.24363657221875984	1.3707390926775683E-4
52-53	9.138260617850453E-5	2.1088293733501046E-4	0.0	0.2748261586506079	1.5113277175675752E-4
54-55	9.138260617850453E-5	2.1088293733501046E-4	0.0	0.3371842432205705	1.6167691862350802E-4
56-57	9.841203742300489E-5	2.1088293733501046E-4	0.0	0.4011626116923904	1.6167691862350802E-4
58-59	9.841203742300489E-5	2.1088293733501046E-4	0.0	0.4521224734993957	1.651916342457582E-4
60-61	1.0192675304525506E-4	2.1439765295726065E-4	0.0	0.48611680299779936	1.6870634986800836E-4
62-63	1.2652976240100627E-4	2.179123685795108E-4	0.0	0.5161816804305274	1.757357811125087E-4
64-65	1.2652976240100627E-4	2.5305952480201253E-4	0.0	0.53077477969411	1.792504967347589E-4
66-67	1.4058862489000698E-4	2.5305952480201253E-4	0.0	0.538711007569151	1.8276521235700906E-4
68-69	1.4058862489000698E-4	2.5305952480201253E-4	0.0	0.5467807946378374	1.8627992797925924E-4
70-71	1.4761805613450733E-4	2.5305952480201253E-4	0.0	0.554108976710229	1.8979464360150943E-4
72-73	1.6870634986800836E-4	2.5305952480201253E-4	0.0	0.5600347872493427	2.179123685795108E-4
74-75	1.6870634986800836E-4	2.636036716687631E-4	0.0	0.5657075382636545	2.179123685795108E-4
76-77	1.6870634986800836E-4	2.811772497800139E-4	0.0	0.5710217882844968	2.179123685795108E-4
78-79	1.6870634986800836E-4	2.882066810245143E-4	0.0	0.5755663155840662	2.179123685795108E-4
80-81	1.6870634986800836E-4	2.882066810245143E-4	0.0	0.5786170887441795	2.2494179982401115E-4
82-83	1.6870634986800836E-4	2.882066810245143E-4	0.0	0.58050800574895	2.319712310685115E-4
84-85	1.6870634986800836E-4	2.882066810245143E-4	0.0	0.5821774956695189	2.319712310685115E-4
86-87	1.6870634986800836E-4	2.9523611226901466E-4	0.0	0.5824727317817879	2.319712310685115E-4
88-89	1.792504967347589E-4	3.0929497475801535E-4	0.0	0.5824938200755214	2.319712310685115E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15850	0.0	46.16069	1
GTATCAA	27595	0.0	33.134583	1
TCAACGC	32825	0.0	26.997688	4
ATCAACG	32940	0.0	26.916183	3
CAACGCA	33875	0.0	26.185656	5
AACGCAG	34715	0.0	25.576239	6
TATCAAC	35555	0.0	25.279387	2
ACGCAGA	39975	0.0	22.158325	7
CGCAGAG	40480	0.0	21.892271	8
GTACATG	35660	0.0	18.373703	1
TACATGG	34835	0.0	18.145718	2
GCAGAGT	49000	0.0	17.931412	9
AGAGTAC	43355	0.0	17.795853	10-11
GAGTACT	28860	0.0	17.558195	12-13
ACATGGG	35035	0.0	17.454529	3
CAGAGTA	48195	0.0	16.479286	10-11
AGTACTT	32110	0.0	15.63717	12-13
GTACTTT	32775	0.0	15.397782	14-15
CATGGGG	25450	0.0	15.215702	4
TATACTG	8795	0.0	14.326306	5
>>END_MODULE