FastQCFastQC Report
Fri 27 May 2016
SRR2139824_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139824_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14865975
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA178200.046.6943741
GTATCAA309150.033.8549081
TCAACGC377300.027.1165494
ATCAACG380000.026.9791183
CAACGCA385900.026.5013645
AACGCAG397100.025.8067676
TATCAAC404350.025.5103452
ACGCAGA456450.022.4236437
CGCAGAG463400.022.0692188
GTACATG384900.018.9679451
GCAGAGT546750.018.4668149
TACATGG384450.018.4004572
AGAGTAC495050.018.02086310-11
GAGTACT326450.017.88216212-13
ACATGGG391650.017.7833653
CAGAGTA535750.016.98096710-11
AGTACTT368350.016.20707512-13
TATACCG11450.016.1329175
CATGGGG280500.015.74521354
GTACTTT373250.015.68032114-15
Produced by FastQC (version 0.11.5)