##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139824_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14865975
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02094420312156	33.0	31.0	34.0	30.0	34.0
2	32.17888688767471	33.0	31.0	34.0	30.0	34.0
3	32.23666076392568	34.0	31.0	34.0	30.0	34.0
4	35.896032248137104	37.0	35.0	37.0	35.0	37.0
5	35.77626068925853	37.0	35.0	37.0	35.0	37.0
6	35.79452615788739	37.0	35.0	37.0	33.0	37.0
7	35.68258476151077	37.0	35.0	37.0	33.0	37.0
8	35.669906750145884	37.0	35.0	37.0	33.0	37.0
9	37.252339923886595	39.0	37.0	39.0	33.0	39.0
10-11	37.17448640267456	39.0	37.0	39.0	33.0	39.0
12-13	37.140745998832905	39.0	37.0	39.0	33.0	39.0
14-15	38.332410622243074	40.0	38.0	41.0	33.0	41.0
16-17	38.33707785732184	40.0	38.0	41.0	33.0	41.0
18-19	38.38338359912485	40.0	38.0	41.0	33.5	41.0
20-21	38.36307023690203	40.0	38.0	41.0	34.0	41.0
22-23	38.28246144614159	40.0	38.0	41.0	33.5	41.0
24-25	38.21329114388842	40.0	38.0	41.0	33.0	41.0
26-27	38.051717954726456	40.0	37.0	41.0	33.0	41.0
28-29	37.95204001898891	40.0	37.0	41.0	33.0	41.0
30-31	37.83362677086959	40.0	37.0	41.0	33.0	41.0
32-33	37.98866424020228	40.0	37.0	41.0	33.0	41.0
34-35	37.97123095432099	40.0	37.0	41.0	33.0	41.0
36-37	37.89701803099828	40.0	37.0	41.0	33.0	41.0
38-39	37.71558187026692	40.0	37.0	41.0	33.0	41.0
40-41	37.741371233475334	40.0	37.0	41.0	33.0	41.0
42-43	37.715986652213616	40.0	37.0	41.0	33.0	41.0
44-45	37.69690183603747	40.0	37.0	41.0	32.5	41.0
46-47	37.72360824423613	40.0	37.0	41.0	32.5	41.0
48-49	37.65075492470274	40.0	37.0	41.0	32.0	41.0
50-51	37.573145202671654	40.0	36.5	41.0	32.0	41.0
52-53	37.44180960577583	39.5	36.0	41.0	32.0	41.0
54-55	37.34862230406587	39.0	36.0	41.0	31.0	41.0
56-57	37.210842078879665	39.0	36.0	41.0	31.0	41.0
58-59	37.07283920025395	39.0	35.0	40.5	31.0	41.0
60-61	36.87741900718056	39.0	35.0	40.0	31.0	41.0
62-63	36.64785472482049	38.0	35.0	40.0	31.0	41.0
64-65	36.45572167244766	38.0	35.0	40.0	31.0	41.0
66-67	36.171322287723974	37.5	34.5	40.0	30.0	41.0
68-69	35.89904383061536	37.0	34.0	40.0	30.0	41.0
70-71	35.613948617915504	36.5	34.0	39.0	30.0	41.0
72-73	35.31492867977603	36.0	34.0	39.0	29.0	40.0
74-75	34.97814639345397	36.0	34.0	38.5	29.0	40.0
76-77	33.90747810943725	35.0	32.5	37.0	28.5	39.0
78-79	34.232998877734374	35.0	33.0	37.0	29.0	39.0
80-81	34.12245778558791	35.0	33.0	37.0	29.0	39.0
82-83	33.90319958937526	35.0	33.0	36.0	29.0	38.5
84-85	33.68061109217976	35.0	33.0	36.0	29.0	37.0
86-87	33.470530767416726	35.0	33.0	35.5	29.0	37.0
88-89	33.2547229556138	35.0	33.0	35.0	29.0	36.5
90-91	33.14811872799612	35.0	33.0	35.0	29.0	36.0
92-93	33.07333075166392	35.0	33.0	35.0	29.0	36.0
94-95	33.022164384264805	34.0	33.0	35.0	29.0	36.0
96-97	32.943367546965334	34.0	32.0	35.0	29.0	35.0
98-99	33.06732419710635	34.0	33.0	35.0	29.0	35.0
100-101	32.60846692337586	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	3.0
19	10.0
20	86.0
21	561.0
22	2585.0
23	9086.0
24	23749.0
25	51323.0
26	91721.0
27	144666.0
28	206858.0
29	273539.0
30	349531.0
31	433274.0
32	539383.0
33	678840.0
34	889794.0
35	1207366.0
36	1797256.0
37	2932772.0
38	3503577.0
39	1662014.0
40	67979.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.8144319494685	22.361856521351612	15.628884079248081	24.194827449931807
2	13.785861138543746	22.901827285869782	41.10802922349684	22.20428235208963
3	16.73603766779439	27.763363043710186	31.840267047635397	23.660332240860026
4	11.996185921205974	19.890548719475177	39.681521057313766	28.43174430200508
5	12.49577642906032	38.44171673906353	36.42131780794735	12.641189023928803
6	28.348857037631237	39.76765735177141	19.23267730505399	12.650808305543363
7	24.836386446230403	33.283703221618495	24.571082623238638	17.308827708912467
8	23.478843466372034	37.597412884119606	21.88566844757912	17.03807520192924
9	24.95685617660463	16.80288040306808	21.90845201878787	36.33181140153942
10-11	23.327272513239127	27.40223227874391	29.90058506085205	19.36991014716492
12-13	24.318838824900485	24.988582989006776	29.812632538397242	20.879945647695493
14-15	21.51658064809069	26.368250316578628	27.31933156082934	24.795837474501337
16-17	21.06032735827956	29.422278054416207	28.017028819165912	21.500365768138316
18-19	21.117057576109204	28.60124546153212	29.624336782484832	20.65736017987384
20-21	21.84776670842802	27.82450922284298	29.45198763768943	20.875736431039567
22-23	21.60255358817366	27.82677170333984	29.257209239678534	21.313465468807966
24-25	21.427524033980614	27.97319946524906	29.301230915042947	21.298045585727376
26-27	21.42163779592767	28.11146434257681	29.20255626095245	21.264341600543073
28-29	21.354082244793883	28.177346998645923	29.204313244086283	21.264257512473907
30-31	21.651887027481038	27.997830533128543	29.110083583283775	21.240198856106645
32-33	21.435050068082344	28.170149430234453	29.05736154919892	21.337438952484284
34-35	21.56904099039162	28.112513341859323	29.095622173945447	21.22282349380361
36-37	21.490271342230297	28.191152212642805	29.009706431228405	21.308870013898495
38-39	21.431976500625023	28.170687221771672	29.078717560541595	21.318618717061707
40-41	21.608208169106184	28.05377366926523	28.970790291908827	21.367227869719756
42-43	21.539602516468797	28.18174518580343	28.757341276111507	21.521311021616263
44-45	21.625838377552903	28.253926162732444	28.585989791298992	21.534245668415657
46-47	21.68639730533987	28.22970613111308	28.351312796149635	21.732583767397415
48-49	21.61089838922542	28.35439351384183	28.29601221655909	21.73869588037366
50-51	21.63649075714401	28.425068192778607	28.210343306495023	21.72809774358236
52-53	21.77124480282597	28.31867104310046	28.154908652695422	21.755175501378158
54-55	21.691274902647294	28.271483345487088	28.174036314185148	21.86320543768047
56-57	21.745088766138775	28.317686185891617	28.111410423596194	21.825814624373415
58-59	21.696468366112658	28.369722773558326	28.15033931140316	21.783469548925858
60-61	21.807949578548104	28.34464310191279	28.12359488716939	21.723812432369712
62-63	21.74337505346698	28.479762085656173	28.051575583582107	21.72528727729474
64-65	21.703048505654213	28.44289185784615	28.019346970834857	21.83471266566478
66-67	21.779874619033787	28.440541546976434	27.894917909145267	21.88466592484451
68-69	21.737397465454183	28.42188222762977	27.930079988078393	21.91064031883765
70-71	21.75945548531597	28.207381675822337	28.001087589365515	22.03207524949618
72-73	21.778034142012412	28.22354498213792	27.931985141279313	22.06643573457035
74-75	21.70378415272806	28.290825033541118	27.940329089016995	22.06506172471383
76-77	21.908683097424788	28.188928310750967	27.890532325805918	22.01185626601833
78-79	21.872717861474936	28.183921499123393	27.849393485520952	22.093967153880723
80-81	21.863881829904035	28.212746794625605	27.822686332994888	22.100685042475472
82-83	21.863517448731212	28.195392023518224	27.91616291462236	22.024927613128206
84-85	21.933302505643198	28.16855325077727	27.902050274512362	21.99609396906717
86-87	21.864293398666522	28.14492619029699	27.975416884395067	22.015363526641423
88-89	21.861653999338078	28.15895057270945	27.94947798176403	22.02991744618844
90-91	21.918434784795014	28.158920745732267	27.98328836641569	21.939356103057023
92-93	21.977865728075123	28.039975040408123	27.993240779965983	21.98891845155077
94-95	21.84220863797871	28.064353948646236	28.0793301648623	22.014107248512758
96-97	21.75269066131858	28.066793704434982	28.19327391333567	21.987241720910763
98-99	21.955222539970016	28.67433258061319	28.25515114749425	21.115293731922545
100-101	22.47923308220931	31.362842582323353	25.502152284346447	20.655772051120888
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3767.0
1	3345.5
2	4343.5
3	6129.5
4	8209.5
5	12559.5
6	16214.0
7	19093.0
8	20923.5
9	20566.5
10	19157.0
11	18115.0
12	20273.0
13	26881.5
14	42102.0
15	63010.5
16	79430.5
17	87502.5
18	89348.0
19	87427.0
20	83280.0
21	79998.5
22	81083.5
23	90317.5
24	108625.5
25	135138.0
26	166801.5
27	200058.0
28	234923.5
29	270431.5
30	302543.5
31	336041.5
32	372197.5
33	405932.5
34	437971.5
35	468937.0
36	502016.0
37	531360.0
38	553513.5
39	571607.0
40	587734.0
41	602575.0
42	612517.5
43	619661.5
44	625486.5
45	627715.0
46	629147.5
47	628500.0
48	619840.0
49	603330.0
50	583088.5
51	557284.5
52	523371.5
53	487442.0
54	450880.0
55	412020.5
56	373204.5
57	333084.0
58	290899.0
59	247969.0
60	205171.5
61	165534.5
62	131063.5
63	100915.0
64	74760.0
65	55457.5
66	41428.0
67	31121.0
68	23741.5
69	17042.5
70	11893.0
71	8528.0
72	5958.0
73	4029.5
74	2722.0
75	1849.0
76	1254.0
77	818.5
78	537.0
79	357.0
80	246.5
81	178.5
82	136.0
83	92.5
84	64.0
85	51.0
86	42.5
87	36.0
88	31.5
89	25.0
90	16.0
91	10.0
92	8.0
93	6.0
94	4.0
95	2.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0017893209157152491
3	8.744801467781293E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0023006502532312953
22-23	4.171237086657316E-4
24-25	0.0011439975257352337
26-27	0.011585891427137113
28-29	3.536129102524372E-4
30-31	0.0024143570890784467
32-33	0.002802526479311405
34-35	0.0017538366448731826
36-37	0.06328935683925663
38-39	0.02807046610883613
40-41	0.02599250960430668
42-43	0.0036763018328910845
44-45	0.0017149788293434778
46-47	0.0036211495643725024
48-49	0.002961986591090301
50-51	7.74137554430411E-4
52-53	9.827773765381336E-4
54-55	0.0015239737719675087
56-57	0.011949658924992999
58-59	0.008988647749039671
60-61	0.004756859673706869
62-63	0.0027192552660824016
64-65	0.00127676481497751
66-67	5.473848844665441E-4
68-69	0.0017421255526365097
70-71	7.26458154880233E-5
72-73	6.700507771833166E-4
74-75	3.6807108254916274E-4
76-77	0.005536325456200337
78-79	0.00164206260034827
80-81	6.755924623159258E-4
82-83	5.150433018291238E-4
84-85	0.0030452002885327273
86-87	0.0037047633242262017
88-89	0.04289732501312234
90-91	4.267745156793478E-4
92-93	5.103411900202024E-4
94-95	2.2661962878933332E-4
96-97	0.018376288195614744
98-99	1.2403484097337994E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	351.0
20-21	1351.0
22-23	2696.0
24-25	5579.0
26-27	7064.0
28-29	12889.0
30-31	35326.0
32-33	46670.0
34-35	66253.0
36-37	102198.0
38-39	106099.0
40-41	113940.0
42-43	130362.0
44-45	153001.0
46-47	153759.0
48-49	138916.0
50-51	126328.0
52-53	115070.0
54-55	125758.0
56-57	132613.0
58-59	137272.0
60-61	138557.0
62-63	134033.0
64-65	144134.0
66-67	155524.0
68-69	153125.0
70-71	152283.0
72-73	147703.0
74-75	150367.0
76-77	152499.0
78-79	163422.0
80-81	165556.0
82-83	159280.0
84-85	160129.0
86-87	169547.0
88-89	180347.0
90-91	195255.0
92-93	203935.0
94-95	251728.0
96-97	323357.0
98-99	1195802.0
100-101	8655897.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.85841237655693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.13198367465058	74.74466642929389
2	7.401937611341346	12.414294731948186
3	1.8672133563523003	4.697446428960184
4	0.7364052363014201	2.4701509592808133
5	0.3332901162817276	1.397459000609186
6	0.182706870523193	0.9192904855418467
7	0.09997917940700905	0.5868866679047914
8	0.06390592647105191	0.4287239628252316
9	0.041124216304915855	0.3103750340604324
>10	0.1379786673642742	1.7788473345539342
>50	0.0029880079213816116	0.17065441804262751
>100	4.596065721326072E-4	0.06313924540576234
>500	2.753050863614814E-5	0.0180653015732175
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.726770359831764E-6	0.0
2	0.0	0.0	0.0	2.018031107949529E-5	0.0
3	0.0	0.0	0.0	2.6907081439327055E-5	0.0
4	0.0	1.3453540719663528E-5	0.0	4.708739251882234E-5	0.0
5	0.0	2.6907081439327055E-5	0.0	6.054093323848587E-5	0.0
6	2.6907081439327055E-5	2.6907081439327055E-5	0.0	6.726770359831763E-5	0.0
7	2.6907081439327055E-5	2.6907081439327055E-5	0.0	6.726770359831763E-5	6.726770359831764E-6
8	2.6907081439327055E-5	2.6907081439327055E-5	0.0	6.726770359831763E-5	1.3453540719663528E-5
9	2.6907081439327055E-5	6.054093323848587E-5	0.0	1.1435509611713998E-4	2.018031107949529E-5
10-11	2.6907081439327055E-5	6.054093323848587E-5	0.0	1.648058738158782E-4	2.018031107949529E-5
12-13	2.6907081439327055E-5	6.390431841840176E-5	0.0	1.681692589957941E-4	2.018031107949529E-5
14-15	3.363385179915882E-5	1.2444525165688762E-4	0.0	1.8834957007528937E-4	2.6907081439327055E-5
16-17	5.717754805856999E-5	1.3453540719663527E-4	0.0	2.4889050331377524E-4	2.6907081439327055E-5
18-19	6.054093323848587E-5	1.5135233309621467E-4	0.0	3.0270466619242934E-4	3.363385179915882E-5
20-21	6.054093323848587E-5	1.6144248863596232E-4	0.0	3.834259105104105E-4	3.363385179915882E-5
22-23	6.054093323848587E-5	1.9171295525520526E-4	0.0	6.121361027446905E-4	3.6997236979074695E-5
24-25	6.390431841840176E-5	2.2871019223427995E-4	0.0	0.001422711931104418	4.708739251882234E-5
26-27	6.726770359831763E-5	2.3543696259411173E-4	0.0	0.003662726460928395	4.708739251882234E-5
28-29	6.726770359831763E-5	2.4889050331377524E-4	0.0	0.01933946478451632	4.708739251882234E-5
30-31	7.39944739581494E-5	2.8925112547276583E-4	0.0	0.028904932236197087	4.708739251882234E-5
32-33	7.39944739581494E-5	3.0270466619242934E-4	0.0	0.03606894266941792	4.708739251882234E-5
34-35	8.072124431798117E-5	3.531554438911676E-4	0.0	0.0454460605510234	4.708739251882234E-5
36-37	8.744801467781293E-5	4.069696067698217E-4	0.0	0.05835809625672046	4.708739251882234E-5
38-39	8.744801467781293E-5	4.237865326694011E-4	0.0	0.08103403913971334	4.708739251882234E-5
40-41	8.744801467781293E-5	4.7087392518822347E-4	0.0	0.1254811742923017	4.708739251882234E-5
42-43	8.744801467781293E-5	4.809640807279711E-4	0.0	0.1450291689579728	4.708739251882234E-5
44-45	9.753817021756057E-5	5.381416287865411E-4	0.0	0.174610141615333	4.708739251882234E-5
46-47	1.0762832575730822E-4	5.785022509455317E-4	0.0	0.20595689149214902	4.708739251882234E-5
48-49	1.0762832575730822E-4	5.818656361254476E-4	0.0	0.2329749646424133	4.708739251882234E-5
50-51	1.109917109372241E-4	6.457699545438493E-4	0.0	0.2581902633362426	4.708739251882234E-5
52-53	1.1435509611713998E-4	6.457699545438493E-4	0.0	0.2919990111647571	5.0450777698738226E-5
54-55	1.1435509611713998E-4	6.625868804434287E-4	0.0	0.36219958663996143	5.717754805856999E-5
56-57	1.1435509611713998E-4	6.894939618827557E-4	0.0	0.4336042540095756	6.054093323848587E-5
58-59	1.1435509611713998E-4	6.928573470626716E-4	0.0	0.4916562822149237	6.726770359831763E-5
60-61	1.2444525165688762E-4	7.029475026024193E-4	0.0	0.5268036573450446	7.39944739581494E-5
62-63	1.479889479162988E-4	7.130376581421669E-4	0.0	0.5475994679124645	7.39944739581494E-5
64-65	1.479889479162988E-4	7.66851821020821E-4	0.0	0.5624252697855338	7.39944739581494E-5
66-67	1.5471571827613056E-4	7.836687469204005E-4	0.0	0.5707025607133067	7.39944739581494E-5
68-69	1.5471571827613056E-4	8.139392135396434E-4	0.0	0.5790235756484187	7.39944739581494E-5
70-71	1.648058738158782E-4	8.341195246191386E-4	0.0	0.586244763629698	7.39944739581494E-5
72-73	1.7489602935562585E-4	8.475730653388022E-4	0.0	0.5921273243093709	8.744801467781293E-5
74-75	1.7489602935562585E-4	8.576632208785499E-4	0.0	0.597518830752776	8.744801467781293E-5
76-77	1.7489602935562585E-4	8.744801467781292E-4	0.0	0.602479823893152	8.744801467781293E-5
78-79	1.7489602935562585E-4	8.81206917137961E-4	0.0	0.6069800332638795	8.744801467781293E-5
80-81	1.7489602935562585E-4	8.81206917137961E-4	0.0	0.6096303807856531	9.08113998577288E-5
82-83	1.7825941453554175E-4	8.81206917137961E-4	0.0	0.6115138764864061	9.417478503764468E-5
84-85	1.816227997154576E-4	8.81206917137961E-4	0.0	0.6131552084542049	9.417478503764468E-5
86-87	1.816227997154576E-4	9.013872282174563E-4	0.0	0.613394008801979	9.753817021756057E-5
88-89	1.816227997154576E-4	9.282943096567834E-4	0.0	0.6134074623426987	1.0426494057739233E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17820	0.0	46.694374	1
GTATCAA	30915	0.0	33.854908	1
TCAACGC	37730	0.0	27.116549	4
ATCAACG	38000	0.0	26.979118	3
CAACGCA	38590	0.0	26.501364	5
AACGCAG	39710	0.0	25.806767	6
TATCAAC	40435	0.0	25.510345	2
ACGCAGA	45645	0.0	22.423643	7
CGCAGAG	46340	0.0	22.069218	8
GTACATG	38490	0.0	18.967945	1
GCAGAGT	54675	0.0	18.466814	9
TACATGG	38445	0.0	18.400457	2
AGAGTAC	49505	0.0	18.020863	10-11
GAGTACT	32645	0.0	17.882162	12-13
ACATGGG	39165	0.0	17.783365	3
CAGAGTA	53575	0.0	16.980967	10-11
AGTACTT	36835	0.0	16.207075	12-13
TATACCG	1145	0.0	16.132917	5
CATGGGG	28050	0.0	15.7452135	4
GTACTTT	37325	0.0	15.680321	14-15
>>END_MODULE