FastQCFastQC Report
Fri 27 May 2016
SRR2139823_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139823_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12541118
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130700.046.7896271
GTATCAA229450.034.488161
TCAACGC280000.027.3265294
ATCAACG283850.027.0302263
CAACGCA287900.026.6646395
AACGCAG294650.026.0824386
TATCAAC306400.025.5099162
GACCGAG120350.023.36554590-91
ACCGAGG122450.023.1740592-93
GAGACCG119750.023.08378488-89
CGAGACC117400.023.03730688-89
AGACCGA119350.023.02233190-91
ACGCAGA345250.022.247567
ACGAGAC125950.022.09956786-87
CGCAGAG347800.022.048048
GCCCACG126450.021.35756782-83
CCCACGA127700.021.06449784-85
CACGAGA129800.020.90289586-87
ATCTCCG127350.020.78546574-75
CCACGAG134500.020.3200384-85
Produced by FastQC (version 0.11.5)