##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139823_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12541118
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.998771002712836	33.0	31.0	34.0	30.0	34.0
2	32.15931243131593	33.0	31.0	34.0	30.0	34.0
3	32.220280600182534	34.0	31.0	34.0	30.0	34.0
4	35.88167330855192	37.0	35.0	37.0	35.0	37.0
5	35.7594334093659	37.0	35.0	37.0	35.0	37.0
6	35.775792237980696	37.0	35.0	37.0	33.0	37.0
7	35.66269235326548	37.0	35.0	37.0	33.0	37.0
8	35.65003423139787	37.0	35.0	37.0	33.0	37.0
9	37.23094264801591	39.0	37.0	39.0	33.0	39.0
10-11	37.150593870498625	39.0	37.0	39.0	33.0	39.0
12-13	37.11743713758215	39.0	37.0	39.0	33.0	39.0
14-15	38.30811710726269	40.0	38.0	41.0	33.0	41.0
16-17	38.310040340901026	40.0	38.0	41.0	33.0	41.0
18-19	38.34940600989481	40.0	38.0	41.0	33.5	41.0
20-21	38.32515212328036	40.0	38.0	41.0	33.5	41.0
22-23	38.24365819671682	40.0	38.0	41.0	33.0	41.0
24-25	38.17300380730681	40.0	37.5	41.0	33.0	41.0
26-27	38.01255078466566	40.0	37.0	41.0	33.0	41.0
28-29	37.91195122547439	40.0	37.0	41.0	33.0	41.0
30-31	37.795501313907806	40.0	37.0	41.0	33.0	41.0
32-33	37.962505283107255	40.0	37.0	41.0	33.0	41.0
34-35	37.94810024095594	40.0	37.0	41.0	33.0	41.0
36-37	37.87224018027294	40.0	37.0	41.0	33.0	41.0
38-39	37.690878242052975	40.0	37.0	41.0	32.0	41.0
40-41	37.71522416566461	40.0	37.0	41.0	33.0	41.0
42-43	37.689181925344116	40.0	37.0	41.0	32.5	41.0
44-45	37.66969341664657	40.0	37.0	41.0	32.5	41.0
46-47	37.695837655481796	40.0	37.0	41.0	32.5	41.0
48-49	37.62031564506231	40.0	37.0	41.0	32.0	41.0
50-51	37.545352379264614	40.0	36.5	41.0	32.0	41.0
52-53	37.40907342449671	39.0	36.0	41.0	31.5	41.0
54-55	37.3092290751313	39.0	36.0	41.0	31.0	41.0
56-57	37.17492061951673	39.0	36.0	41.0	31.0	41.0
58-59	37.034330444775954	39.0	35.0	40.0	31.0	41.0
60-61	36.84151003145101	39.0	35.0	40.0	31.0	41.0
62-63	36.60944319617835	38.0	35.0	40.0	31.0	41.0
64-65	36.41599126017738	38.0	35.0	40.0	30.5	41.0
66-67	36.13648138376766	37.5	34.5	40.0	30.0	41.0
68-69	35.86159863549372	37.0	34.0	40.0	30.0	41.0
70-71	35.58195984036193	36.5	34.0	39.0	30.0	41.0
72-73	35.28066164755527	36.0	34.0	39.0	29.0	40.0
74-75	34.94680763524028	36.0	34.0	38.5	29.0	40.0
76-77	33.882571215307195	35.0	32.5	37.0	28.0	39.0
78-79	34.213157127105696	35.0	33.0	37.0	29.0	39.0
80-81	34.09730404633352	35.0	33.0	37.0	29.0	39.0
82-83	33.873718856040604	35.0	33.0	36.0	29.0	38.5
84-85	33.65414596210063	35.0	33.0	36.0	29.0	37.0
86-87	33.4502597237022	35.0	33.0	35.5	29.0	37.0
88-89	33.23234640867731	35.0	33.0	35.0	29.0	36.5
90-91	33.121251675314404	35.0	33.0	35.0	29.0	36.0
92-93	33.05109901713334	34.5	33.0	35.0	29.0	36.0
94-95	32.99526866988046	34.0	33.0	35.0	29.0	36.0
96-97	32.91372661417187	34.0	32.0	35.0	29.0	35.0
98-99	33.04474420210147	34.0	32.5	35.0	29.0	35.0
100-101	32.59641557985189	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	4.0
19	14.0
20	70.0
21	486.0
22	2252.0
23	7830.0
24	21044.0
25	44321.0
26	79776.0
27	124889.0
28	178442.0
29	236733.0
30	299789.0
31	373002.0
32	460055.0
33	580822.0
34	759670.0
35	1027354.0
36	1532547.0
37	2480768.0
38	2928313.0
39	1352810.0
40	50124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.63646909310637	22.455892688355217	15.555925715713704	24.351712502824707
2	13.719047106058262	22.860405788401778	41.10883526351559	22.31171184202437
3	16.795207950940856	27.67856936393255	31.69275248189813	23.833470203228472
4	12.042227814139059	19.740895508677934	39.656847180610214	28.560029496572792
5	12.51767186944577	38.39246229881578	36.41900187846092	12.67086395327753
6	28.51119812444154	39.73522934717623	19.1967414707365	12.556831057645738
7	24.986719684800033	33.099273924382175	24.630300105620567	17.28370628519722
8	23.454176892363186	37.734235496388756	21.883878295380043	16.92770931586801
9	24.947098017896014	16.790193665349452	21.86328204550822	36.39942627124631
10-11	23.371472942045518	27.36851690574955	29.9403011757006	19.319708976504327
12-13	24.250772538780037	24.921825151473737	29.82266413568551	21.004738174060716
14-15	21.47609965873856	26.34791013050033	27.218474461367798	24.957515749393316
16-17	21.16169387769097	29.36523282852454	27.91031868131693	21.562754612467565
18-19	21.209847479307665	28.636956449975195	29.420207991025997	20.732988079691143
20-21	21.94889443329998	27.860628518042073	29.210765627387293	20.979711421270654
22-23	21.679410821077845	27.82613629738964	29.073136408162135	21.42131647337038
24-25	21.502834371998816	27.93984954848952	29.125603746622232	21.431712332889433
26-27	21.546250747303063	28.025654631898323	29.017310136737606	21.410784484061008
28-29	21.46705362254589	28.091317851106297	29.020405559588674	21.421222966759142
30-31	21.724648821263127	27.966903149816797	28.93046477765504	21.377983251265036
32-33	21.58042851052343	28.092500244576314	28.893897306151135	21.433173938749118
34-35	21.60745208476139	28.106818380572317	28.938731961454323	21.346997573211965
36-37	21.62171651009477	28.08628102946568	28.863060782755106	21.428941677684442
38-39	21.541482462607814	28.13596811390633	28.929285568175395	21.393263855310465
40-41	21.654491551677573	28.027111292297153	28.818807384063742	21.49958977196153
42-43	21.608052101737695	28.160072291734544	28.629983419391287	21.601892187136475
44-45	21.69511319633326	28.214453966680146	28.453350361340007	21.637082475646583
46-47	21.7588312630815	28.196442883136825	28.232279100097816	21.812446753683858
48-49	21.675756074219137	28.294270789615872	28.23686176823101	21.793111367933985
50-51	21.65501120598291	28.39180212601129	28.1439186364855	21.809268031520304
52-53	21.840338568956877	28.274457489623778	28.07036935637258	21.814834585046764
54-55	21.752843510025276	28.20495015829611	28.135206382873513	21.9069999488051
56-57	21.805037145958877	28.266843975341676	28.09565187394266	21.832467004756786
58-59	21.755755927666716	28.351478646518324	28.084055141582382	21.80871028423258
60-61	21.75208794522233	28.173932334361655	28.170798928160217	21.903180792255796
62-63	21.915963234627583	28.247593421980877	28.139864417782995	21.696578925608545
64-65	21.652687993407785	28.192723564128645	28.133748286990773	22.020840155472797
66-67	21.72137792541725	28.266327686665782	27.978773045968808	22.033521341948155
68-69	21.696133799593873	28.445982431740642	28.03550134506328	21.822382423602207
70-71	21.711028668174123	28.23862022339132	28.062213801174906	21.98813730725965
72-73	21.714589102357422	28.228929891477495	27.81136532409344	22.245115682071646
74-75	21.62493857810818	28.28908047281423	27.82847690585365	22.257504043223943
76-77	21.798820929712875	28.03615410945468	27.9598395479224	22.205185412910044
78-79	21.77679877851345	28.033808732785293	27.91926203876801	22.270130449933244
80-81	21.998974100914843	28.03543351145608	27.68526009154215	22.28033229608692
82-83	22.001368362233652	28.253990042193383	27.733392549248205	22.01124904632476
84-85	21.834159561697295	27.985160451875224	27.756129410134754	22.424550576292727
86-87	21.772260636890767	28.20038646422261	27.81000942546921	22.21734347341741
88-89	22.071646540485666	27.925778613219837	27.80642220085895	22.196152645435546
90-91	22.087648900472185	28.182649254795102	27.836710623524162	21.89299122120855
92-93	21.84784846892127	28.094384419511186	27.838327528599134	22.219439582968413
94-95	21.992064913156977	27.856605022748465	27.93293453804476	22.218395526049804
96-97	21.9496311487822	28.14201054184905	28.018974239625287	21.889384069743464
98-99	22.162765322927406	28.54521006720242	28.192712128764924	21.099312481105247
100-101	22.506062359245323	31.328145172731663	25.47434146304163	20.69145100498138
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2165.0
1	2004.0
2	2943.5
3	4287.0
4	5786.5
5	9083.5
6	11889.5
7	14030.5
8	15393.5
9	15166.5
10	14234.5
11	13507.0
12	14982.5
13	19565.0
14	30649.0
15	46319.0
16	58749.0
17	64932.0
18	66292.5
19	65070.5
20	62739.5
21	61461.5
22	64015.5
23	73031.5
24	89044.0
25	112021.0
26	140326.5
27	169574.0
28	198502.0
29	226971.5
30	253523.0
31	281346.0
32	310442.5
33	339214.0
34	367895.5
35	394037.5
36	421826.0
37	445739.5
38	463150.0
39	478124.0
40	493035.0
41	507831.0
42	515109.0
43	519108.0
44	523960.5
45	527069.0
46	524820.5
47	518168.5
48	509596.5
49	498574.5
50	485038.5
51	464555.0
52	437632.0
53	410365.5
54	382712.0
55	352596.0
56	321487.0
57	289071.0
58	254792.5
59	218988.5
60	182476.0
61	147765.0
62	116479.5
63	89158.0
64	65875.5
65	48843.0
66	36772.5
67	27725.5
68	20702.0
69	14886.5
70	10835.5
71	7831.0
72	5497.5
73	3854.5
74	2636.5
75	1789.0
76	1226.0
77	808.5
78	551.0
79	398.0
80	280.0
81	187.5
82	136.0
83	98.0
84	69.5
85	59.0
86	50.0
87	39.0
88	28.5
89	22.0
90	15.5
91	9.5
92	6.5
93	3.5
94	2.5
95	3.0
96	2.5
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0019695213775996687
3	8.771147835464111E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.002336402206935223
22-23	4.625468258880441E-4
24-25	0.0012164624299367496
26-27	0.011969781568244074
28-29	2.953974836605273E-4
30-31	0.0026416865166162084
32-33	0.0028322777590361376
34-35	0.0016999766303449045
36-37	0.06308318076993377
38-39	0.02756861448421098
40-41	0.025542889783106107
42-43	0.0034078473317401166
44-45	0.0016444334408487508
46-47	0.0036306095810314408
48-49	0.0030386587946562784
50-51	6.907294497692105E-4
52-53	9.953941812891514E-4
54-55	0.0013966017798205793
56-57	0.012360852853033294
58-59	0.009311304675720802
60-61	0.004708840149110884
62-63	0.002695866458717057
64-65	0.0012111174180621939
66-67	5.222776611999602E-4
68-69	0.0014546069553785519
70-71	5.681363027166574E-5
72-73	5.654777736085773E-4
74-75	2.3278673069077716E-4
76-77	0.0045122839688812475
78-79	0.0013230142203138986
80-81	7.413530995216608E-4
82-83	5.523333270258231E-4
84-85	0.003017636999423538
86-87	0.00347092610303665
88-89	0.04209863511568688
90-91	3.6925470228215155E-4
92-93	6.028605678636823E-4
94-95	2.1443999418754752E-4
96-97	0.017994447080374905
98-99	1.6973800514560763E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	279.0
20-21	1087.0
22-23	2210.0
24-25	4623.0
26-27	5665.0
28-29	9967.0
30-31	27923.0
32-33	36480.0
34-35	51429.0
36-37	81033.0
38-39	85668.0
40-41	91262.0
42-43	103761.0
44-45	123099.0
46-47	122965.0
48-49	112621.0
50-51	104437.0
52-53	94952.0
54-55	103712.0
56-57	110617.0
58-59	66772.0
60-61	118603.0
62-63	112560.0
64-65	119366.0
66-67	127698.0
68-69	128912.0
70-71	128961.0
72-73	123548.0
74-75	126258.0
76-77	127961.0
78-79	137679.0
80-81	138673.0
82-83	133864.0
84-85	134218.0
86-87	141128.0
88-89	152968.0
90-91	165758.0
92-93	173972.0
94-95	213151.0
96-97	274797.0
98-99	1062450.0
100-101	7358031.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.03170666272236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.39481740441944	77.76820414767364
2	6.804230698424514	11.707591590246963
3	1.5893638740736487	4.102070597838964
4	0.5711218200526045	1.9653833956578308
5	0.24462962134646316	1.052295191234588
6	0.13153760526270633	0.6789842802646869
7	0.0722698323220058	0.43522479104236533
8	0.046709872676849895	0.3214824051510288
9	0.03150755721269749	0.2439584027803559
>10	0.11055574842691052	1.4743568876871276
>50	0.002700617051790662	0.15288512596641243
>100	5.106845496586963E-4	0.07215338225466417
>500	4.466418065985927E-5	0.025409802201395947
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.973770759512829E-6	1.5947541519025657E-5
2	0.0	1.5947541519025657E-5	0.0	1.5947541519025657E-5	1.5947541519025657E-5
3	7.973770759512829E-6	1.5947541519025657E-5	0.0	4.784262455707697E-5	1.5947541519025657E-5
4	7.973770759512829E-6	8.771147835464111E-5	0.0	4.784262455707697E-5	1.5947541519025657E-5
5	2.3921312278538484E-5	8.771147835464111E-5	0.0	4.784262455707697E-5	1.5947541519025657E-5
6	3.1895083038051314E-5	8.771147835464111E-5	0.0	4.784262455707697E-5	3.1895083038051314E-5
7	3.1895083038051314E-5	8.771147835464111E-5	0.0	4.784262455707697E-5	3.1895083038051314E-5
8	3.1895083038051314E-5	9.568524911415394E-5	0.0	4.784262455707697E-5	3.1895083038051314E-5
9	3.1895083038051314E-5	1.8339672746879506E-4	0.0	7.973770759512828E-5	3.1895083038051314E-5
10-11	3.1895083038051314E-5	1.8339672746879506E-4	0.0	9.568524911415394E-5	3.1895083038051314E-5
12-13	3.1895083038051314E-5	1.8339672746879506E-4	0.0	1.0365901987366676E-4	3.588196841780773E-5
14-15	3.1895083038051314E-5	2.910426327222182E-4	0.0	1.395409882914745E-4	4.3855739177320555E-5
16-17	4.784262455707697E-5	3.0300328886148745E-4	0.0	1.8339672746879506E-4	4.784262455707697E-5
18-19	4.784262455707697E-5	3.269246011400259E-4	0.0	2.1529181050684637E-4	5.980328069634621E-5
20-21	5.182950993683338E-5	3.628065695578337E-4	0.0	2.511737789246541E-4	6.379016607610263E-5
22-23	5.980328069634621E-5	4.505180479124748E-4	0.0	4.584918186719876E-4	7.176393683561546E-5
24-25	6.379016607610263E-5	5.222819847480902E-4	0.0	9.72800032660565E-4	8.771147835464111E-5
26-27	7.176393683561546E-5	5.58163953165898E-4	0.0	0.0026233705798797205	8.771147835464111E-5
28-29	7.176393683561546E-5	5.58163953165898E-4	0.0	0.015233889036049258	9.169836373439753E-5
30-31	7.176393683561546E-5	6.339147753812698E-4	0.0	0.023008315526574265	9.568524911415394E-5
32-33	7.176393683561546E-5	6.418885461407827E-4	0.0	0.02826303045709322	1.0365901987366676E-4
34-35	7.176393683561546E-5	7.61495107533475E-4	0.0	0.03507661757109693	1.0365901987366676E-4
36-37	7.176393683561546E-5	8.77114783546411E-4	0.0	0.04450560149422085	1.0365901987366676E-4
38-39	7.176393683561546E-5	8.811016689261675E-4	0.0	0.06251436275458057	1.0365901987366676E-4
40-41	7.176393683561546E-5	9.887475741795907E-4	0.0	0.10197655424340957	1.0365901987366676E-4
42-43	7.176393683561546E-5	0.0010126688864581291	0.0	0.11901650235648847	1.0365901987366676E-4
44-45	7.973770759512828E-5	0.0011282885624710652	0.0	0.14338833268293943	1.0365901987366676E-4
46-47	8.771147835464111E-5	0.0012120131554459498	0.0	0.1708779073763599	1.0365901987366676E-4
48-49	8.771147835464111E-5	0.0012120131554459498	0.0	0.19534143606654525	1.1163279063317959E-4
50-51	9.169836373439753E-5	0.0012797902069018089	0.0	0.21834576470773975	1.1163279063317959E-4
52-53	9.568524911415394E-5	0.0012837770922815653	0.0	0.2502567952873101	1.2359344677244884E-4
54-55	9.568524911415394E-5	0.0012957377484208346	0.0	0.3133293219950566	1.3555410291171807E-4
56-57	9.568524911415394E-5	0.0013236459460791296	0.0	0.38026115375040725	1.3555410291171807E-4
58-59	9.568524911415394E-5	0.0013316197168386423	0.0	0.43640846055351684	1.3555410291171807E-4
60-61	9.967213449391034E-5	0.0013635147998766937	0.0	0.847113471063744	1.3555410291171807E-4
62-63	1.1960656139269242E-4	0.0013914229975349884	0.0	0.8578302189645293	1.3555410291171807E-4
64-65	1.1960656139269242E-4	0.001570832839624027	0.0	0.8679569078291106	1.3555410291171807E-4
66-67	1.435278736712309E-4	0.0015867803811430528	0.0	0.8767838720598913	1.3555410291171807E-4
68-69	1.435278736712309E-4	0.0016346230057001298	0.0	0.8856706395713683	1.3555410291171807E-4
70-71	1.4751475905098733E-4	0.0016705049741179375	0.0	0.894186626742528	1.5150164443074372E-4
72-73	1.5150164443074372E-4	0.0016904394010167196	0.0	0.9007530269629869	1.5947541519025657E-4
74-75	1.5548852981050014E-4	0.0017382820255737964	0.0	0.9065061025659754	1.5947541519025657E-4
76-77	1.6744918594976938E-4	0.0018100459624094119	0.0	0.9111707584602904	1.5947541519025657E-4
78-79	1.6744918594976938E-4	0.0018419410454474632	0.0	0.9158952176353017	1.5947541519025657E-4
80-81	1.6744918594976938E-4	0.0018618754723462453	0.0	0.9186979980572705	1.714360713295258E-4
82-83	1.6744918594976938E-4	0.0018738361284855146	0.0	0.9208788243599972	2.033311543675771E-4
84-85	1.6744918594976938E-4	0.0019216787530425916	0.0	0.9225453424487354	2.1529181050684637E-4
86-87	1.7940984208903864E-4	0.00194560006532113	0.0	0.9227725949153815	2.2326558126635918E-4
88-89	1.9137049822830787E-4	0.0020253377729162582	0.0	0.9228284113106981	2.2326558126635918E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13070	0.0	46.789627	1
GTATCAA	22945	0.0	34.48816	1
TCAACGC	28000	0.0	27.326529	4
ATCAACG	28385	0.0	27.030226	3
CAACGCA	28790	0.0	26.664639	5
AACGCAG	29465	0.0	26.082438	6
TATCAAC	30640	0.0	25.509916	2
GACCGAG	12035	0.0	23.365545	90-91
ACCGAGG	12245	0.0	23.17405	92-93
GAGACCG	11975	0.0	23.083784	88-89
CGAGACC	11740	0.0	23.037306	88-89
AGACCGA	11935	0.0	23.022331	90-91
ACGCAGA	34525	0.0	22.24756	7
ACGAGAC	12595	0.0	22.099567	86-87
CGCAGAG	34780	0.0	22.04804	8
GCCCACG	12645	0.0	21.357567	82-83
CCCACGA	12770	0.0	21.064497	84-85
CACGAGA	12980	0.0	20.902895	86-87
ATCTCCG	12735	0.0	20.785465	74-75
CCACGAG	13450	0.0	20.32003	84-85
>>END_MODULE