FastQCFastQC Report
Fri 27 May 2016
SRR2139822_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139822_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11896918
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126500.045.0442921
GTATCAA217600.034.7707861
TATCTCG168850.028.85528294-95
TCAACGC263600.027.9884664
ATCAACG268200.027.604053
CAACGCA272800.027.0445735
AACGCAG280600.026.3080336
TATCAAC285000.026.1578332
ACGCAGA328100.022.4602587
CGCAGAG330300.022.2847778
GCTATCT252850.021.48106894-95
CTATCTC256400.020.80218394-95
CCGTCTT87300.020.46712192-93
TATGCCG84800.020.27354888-89
TGCCGTC89300.019.3451790-91
GTACATG291050.019.3280681
GCCGTCT87650.019.2965890-91
CGCTATC250950.018.71057192-93
ATGCCGT88950.018.56778788-89
TACATGG296800.018.564752
Produced by FastQC (version 0.11.5)