##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139822_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11896918
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03545044187074	33.0	31.0	34.0	30.0	34.0
2	32.19736430897481	33.0	31.0	34.0	30.0	34.0
3	32.26093665603142	34.0	31.0	34.0	30.0	34.0
4	35.91113849822282	37.0	35.0	37.0	35.0	37.0
5	35.79173749033153	37.0	35.0	37.0	35.0	37.0
6	35.80760857559916	37.0	35.0	37.0	35.0	37.0
7	35.695111204431264	37.0	35.0	37.0	33.0	37.0
8	35.683218292334196	37.0	35.0	37.0	33.0	37.0
9	37.27095210709194	39.0	37.0	39.0	34.0	39.0
10-11	37.1920378454319	39.0	37.0	39.0	33.0	39.0
12-13	37.15958128819582	39.0	37.0	39.0	33.0	39.0
14-15	38.363342926294024	40.0	38.0	41.0	33.0	41.0
16-17	38.36348258431301	40.0	38.0	41.0	33.0	41.0
18-19	38.40240215995437	40.0	38.0	41.0	33.5	41.0
20-21	38.37860213649859	40.0	38.0	41.0	34.0	41.0
22-23	38.2987509194749	40.0	38.0	41.0	33.5	41.0
24-25	38.22786287266187	40.0	38.0	41.0	33.0	41.0
26-27	38.067516495358845	40.0	37.0	41.0	33.0	41.0
28-29	37.96829327306857	40.0	37.0	41.0	33.0	41.0
30-31	37.85339365019644	40.0	37.0	41.0	33.0	41.0
32-33	38.020731798642956	40.0	37.0	41.0	33.0	41.0
34-35	38.00363641091795	40.0	37.0	41.0	33.0	41.0
36-37	37.928849641941525	40.0	37.0	41.0	33.0	41.0
38-39	37.755612215782975	40.0	37.0	41.0	33.0	41.0
40-41	37.78024042252278	40.0	37.0	41.0	33.0	41.0
42-43	37.75208867148858	40.0	37.0	41.0	33.0	41.0
44-45	37.73162132605678	40.0	37.0	41.0	33.0	41.0
46-47	37.75612230022443	40.0	37.0	41.0	33.0	41.0
48-49	37.680155383220225	40.0	37.0	41.0	32.0	41.0
50-51	37.59534746936761	40.0	36.5	41.0	32.0	41.0
52-53	37.46159013788646	39.5	36.0	41.0	32.0	41.0
54-55	37.37672429531622	39.0	36.0	41.0	31.5	41.0
56-57	37.24060495495663	39.0	36.0	41.0	31.0	41.0
58-59	37.10972808569651	39.0	35.5	40.5	31.0	41.0
60-61	36.91605957712372	39.0	35.0	40.0	31.0	41.0
62-63	36.687764859002854	38.0	35.0	40.0	31.0	41.0
64-65	36.51061895979278	38.0	35.0	40.0	31.0	41.0
66-67	36.22986470113417	37.5	35.0	40.0	30.0	41.0
68-69	35.9550929884318	37.0	34.0	40.0	30.0	41.0
70-71	35.66734129499268	36.5	34.0	39.0	30.0	41.0
72-73	35.3687241936793	36.0	34.0	39.0	30.0	40.5
74-75	35.01842871194676	36.0	34.0	38.5	29.0	40.0
76-77	33.92109036132874	35.0	32.5	37.0	28.0	39.0
78-79	34.211623853694334	35.0	33.0	37.0	29.0	39.0
80-81	34.07458337551395	35.0	33.0	37.0	29.0	39.0
82-83	33.84766177702242	35.0	33.0	36.0	29.0	38.5
84-85	33.63812310063706	35.0	33.0	36.0	29.0	37.0
86-87	33.43928606089738	35.0	33.0	35.0	29.0	37.0
88-89	33.220114980512164	35.0	33.0	35.0	29.0	36.5
90-91	33.12526939989756	35.0	33.0	35.0	29.0	36.0
92-93	33.06892205439623	35.0	33.0	35.0	29.0	36.0
94-95	33.01336157819472	34.0	33.0	35.0	29.0	35.5
96-97	32.92177725421334	34.0	32.0	35.0	29.0	35.0
98-99	33.03787354661805	34.0	32.5	35.0	29.0	35.0
100-101	32.57762599332	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	5.0
20	57.0
21	468.0
22	2057.0
23	7341.0
24	19349.0
25	40915.0
26	73943.0
27	116575.0
28	167094.0
29	222079.0
30	281260.0
31	349453.0
32	431956.0
33	542970.0
34	710580.0
35	965686.0
36	1444444.0
37	2372859.0
38	2869425.0
39	1227547.0
40	50852.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.86560519287433	22.282905539064824	15.47877357816537	24.372715689895482
2	13.762350720882155	22.75944520291974	41.04554768837072	22.432656387827386
3	16.793467141793002	27.587861919729384	31.71309414397323	23.905576794504384
4	12.050541156961827	19.769615962722444	39.52660680690579	28.65323607340994
5	12.587394483176231	38.29744812900282	36.44017719547197	12.674980192348976
6	28.527464003702473	39.60956947000896	19.19745937561308	12.665507150675495
7	25.011284435178926	33.08134089854196	24.456796289593658	17.450578376685456
8	23.53280067997443	37.43180376631998	21.94764223809898	17.087753315606612
9	24.968533867342785	16.736897741078824	21.810396608600648	36.48417178297774
10-11	23.364433544889526	27.258278152375265	29.928074649249492	19.449213653485717
12-13	24.29622108852057	24.823584561984877	29.776140341557365	21.104054007937183
14-15	21.503354902505002	26.285320282110035	27.135620334611033	25.07570448077393
16-17	21.223988431289513	29.3239307861078	27.849506905906217	21.602573876696468
18-19	21.28603811508157	28.5883789398229	29.30744332271602	20.818139622379512
20-21	21.957062621049364	27.79141865220091	29.12787969870963	21.1236390280401
22-23	21.699564563418416	27.820249667344477	28.94839072741011	21.531795041827
24-25	21.56279764289739	27.989702160128022	28.96795322255051	21.47954697442408
26-27	21.606033768221682	28.067500268681115	28.865673531513085	21.460792431584114
28-29	21.553002867219327	28.048027428086293	28.950974921477474	21.4479947832169
30-31	21.703077475666657	27.876653597718548	28.93998116541382	21.480287761200973
32-33	21.50106713425394	28.112654378269774	28.81870997718692	21.567568510289366
34-35	21.688275203367922	28.080222161185826	28.816035552161694	21.415467083284558
36-37	21.61584223502091	28.084711418679458	28.862805045843032	21.4366413004566
38-39	21.5614402963954	28.113476728329946	28.84705963070643	21.47802334456823
40-41	21.649606084336277	27.949659159533724	28.80181740228075	21.598917353849252
42-43	21.733088201916445	28.016426593935012	28.630134655922756	21.62035054822579
44-45	21.631552720478933	28.098316539237366	28.49767012729037	21.77246061299333
46-47	21.74264094923203	28.015872194459064	28.2419006720215	21.999586184287406
48-49	21.66216682377922	28.249440054793283	28.257793778807503	21.830599342619994
50-51	21.622354524756126	28.325274824754544	28.221263868146334	21.831106782342996
52-53	21.728168656932283	28.17656025194022	28.00022679485933	22.09504429626817
54-55	21.660229290048456	27.95205935421911	28.124440689434394	22.263270666298038
56-57	21.75357016679267	27.67243097533269	28.342535559300885	22.231463298573757
58-59	21.53162672383102	27.511416905686893	28.564660597517626	22.39229577296446
60-61	21.653839830844117	27.370291860610248	28.743203460093337	22.232664848452302
62-63	21.40261138842777	27.918401230065204	28.71635556607052	21.962631815436513
64-65	21.01157039765919	28.10561174205773	28.526555093860928	22.35626276642215
66-67	21.11350175161392	28.621050065294163	28.072031025411547	22.193417157680372
68-69	21.207594567156747	28.896898112654718	27.66962712092737	22.225880199261166
70-71	21.218163745242077	28.507314471355492	27.633966106625934	22.64055567677649
72-73	21.11015762856163	28.10344174374302	27.584561513300148	23.201839114395202
74-75	21.545195220937572	27.738900972980744	27.657703358578477	23.058200447503204
76-77	21.94658443445938	27.583462041167028	27.556397063042542	22.91355646133105
78-79	22.137844088102852	27.467027649300107	26.906604657796613	23.488523604800424
80-81	22.031099706892533	27.856509291721075	26.83425083812313	23.27814016326326
82-83	21.982611576953794	27.98108290711595	26.939886684481422	23.096418831448833
84-85	21.983470319789834	28.001135160064788	26.89809124937254	23.117303270772833
86-87	22.320102901879967	28.271653984674778	26.81560401385393	22.59263909959132
88-89	22.49605035072623	27.94786813017554	27.160842670608332	22.3952388484899
90-91	22.209631989466974	27.95695619130826	27.444463981757995	22.388947837466773
92-93	21.99284817916471	27.42925546557396	27.358338421816136	23.21955793344519
94-95	21.82986701207465	27.42881734566467	27.999555844627384	22.741759797633296
96-97	21.444683985634956	27.728405197764065	28.246521556011494	22.580389260589488
98-99	21.661313324296515	27.836416502316364	28.63892562731066	21.863344546076462
100-101	22.331276264422588	30.776491260271683	25.94563156125625	20.94660091404948
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2278.0
1	2080.0
2	2911.5
3	4200.5
4	5721.0
5	8865.0
6	11412.0
7	13221.5
8	14333.5
9	14156.5
10	13272.5
11	12528.5
12	14140.5
13	18728.5
14	29125.5
15	43475.5
16	54501.5
17	60128.5
18	61580.0
19	59948.0
20	56812.0
21	54471.5
22	55023.0
23	61228.0
24	73883.0
25	92567.5
26	116467.0
27	142447.5
28	170207.5
29	198141.0
30	223830.0
31	250279.0
32	277673.5
33	303709.0
34	328001.0
35	351496.0
36	378063.5
37	403457.0
38	425529.5
39	443737.0
40	459490.0
41	475809.0
42	484712.5
43	490364.5
44	498509.5
45	505560.0
46	508409.0
47	506201.5
48	503449.5
49	493880.5
50	477272.0
51	456546.0
52	430415.5
53	401183.0
54	368254.0
55	334600.5
56	300194.5
57	264993.5
58	231186.0
59	196889.5
60	162330.0
61	131031.0
62	103245.0
63	78627.5
64	57725.0
65	42489.5
66	31838.5
67	24237.5
68	18722.5
69	13743.0
70	9719.5
71	7027.0
72	5036.0
73	3597.0
74	2527.0
75	1741.0
76	1219.0
77	827.0
78	562.5
79	391.5
80	274.0
81	199.5
82	156.0
83	109.5
84	68.0
85	48.0
86	35.0
87	25.0
88	18.5
89	14.0
90	10.5
91	8.5
92	6.5
93	3.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002050951347231274
3	1.0086645969989876E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0021350843490709924
22-23	3.7409945539105687E-4
24-25	0.001151983252686097
26-27	0.011154017252640215
28-29	2.3564170896961327E-4
30-31	0.0024012383312455197
32-33	0.002537865930919205
34-35	0.0015675460020734822
36-37	0.06402199914918301
38-39	0.029553616922879465
40-41	0.026338966739905587
42-43	0.0035844931873799456
44-45	0.001685525065355687
46-47	0.0035780775215041795
48-49	0.0028098100340594543
50-51	7.348472689829534E-4
52-53	0.0010259229856864679
54-55	0.001527849420981447
56-57	0.01164768402406939
58-59	0.008597280256779406
60-61	0.004618327318817246
62-63	0.002648218399497357
64-65	0.0012539337492525524
66-67	5.395595859078963E-4
68-69	0.0016623504639107546
70-71	5.33371839547592E-5
72-73	7.115897705584297E-4
74-75	2.284593387572229E-4
76-77	0.005496171536871366
78-79	0.0014822820684976298
80-81	5.474270386918848E-4
82-83	4.3984278133954436E-4
84-85	0.0026116917583713746
86-87	0.0031122025477416182
88-89	0.04043732673622441
90-91	4.0581608948500494E-4
92-93	3.8492500104297695E-4
94-95	1.9632414224538694E-4
96-97	0.019991231105621107
98-99	1.9193857965451054E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	262.0
20-21	997.0
22-23	2057.0
24-25	4259.0
26-27	5309.0
28-29	9208.0
30-31	26242.0
32-33	35445.0
34-35	50507.0
36-37	77884.0
38-39	54445.0
40-41	85832.0
42-43	96423.0
44-45	113733.0
46-47	114665.0
48-49	104586.0
50-51	95786.0
52-53	18755.0
54-55	19105.0
56-57	19468.0
58-59	20683.0
60-61	114057.0
62-63	112222.0
64-65	118174.0
66-67	128794.0
68-69	124789.0
70-71	124647.0
72-73	120619.0
74-75	123959.0
76-77	125009.0
78-79	133888.0
80-81	133605.0
82-83	129962.0
84-85	131516.0
86-87	138428.0
88-89	148463.0
90-91	159746.0
92-93	167340.0
94-95	207089.0
96-97	266619.0
98-99	1076852.0
100-101	7155489.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.3913576554401
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.17745458327788	75.25806464539168
2	7.50806751110277	12.672320212613275
3	1.8916627400276598	4.789199605714321
4	0.6468065882679409	2.1833954449765915
5	0.31901917064016266	1.3461230464217921
6	0.15224095073577792	0.7708692314008363
7	0.0845556713532854	0.4995037532079672
8	0.05842265588283075	0.3944293798230938
9	0.03485910001964794	0.26476260995743817
>10	0.12417118239995001	1.6268967002586479
>50	0.0024251872178499417	0.1340253919164892
>100	2.819052067450643E-4	0.044074515445021945
>500	3.275386742444005E-5	0.016335462872802003
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.405538308324895E-6	0.0	8.405538308324895E-6	0.0
3	0.0	8.405538308324895E-6	0.0	8.405538308324895E-6	0.0
4	0.0	4.202769154162448E-5	0.0	3.362215323329958E-5	0.0
5	0.0	5.043322984994937E-5	0.0	5.043322984994937E-5	0.0
6	0.0	5.043322984994937E-5	0.0	5.043322984994937E-5	0.0
7	0.0	5.043322984994937E-5	0.0	6.724430646659916E-5	0.0
8	0.0	5.883876815827427E-5	0.0	7.564984477492407E-5	8.405538308324895E-6
9	0.0	8.405538308324895E-5	0.0	1.3448861293319833E-4	8.405538308324895E-6
10-11	0.0	8.405538308324895E-5	0.0	1.7231353532066036E-4	8.405538308324895E-6
12-13	0.0	8.82581522374114E-5	0.0	1.849218427831477E-4	8.405538308324895E-6
14-15	0.0	1.5129968954984813E-4	0.0	1.933273810914726E-4	1.2608307462487343E-5
16-17	0.0	1.8071907362898526E-4	0.0	2.3535507263309707E-4	1.681107661664979E-5
18-19	0.0	1.8912461193731016E-4	0.0	2.6057168755807175E-4	1.681107661664979E-5
20-21	0.0	1.933273810914726E-4	0.0	3.446270706413207E-4	1.681107661664979E-5
22-23	0.0	2.2274676517060972E-4	0.0	6.0099598904523E-4	2.101384577081224E-5
24-25	0.0	2.6057168755807175E-4	0.0	0.0012944528994820338	2.5216614924974686E-5
26-27	0.0	2.7738276417472156E-4	0.0	0.003669017471583817	2.5216614924974686E-5
28-29	0.0	2.7738276417472156E-4	0.0	0.018462764894235633	2.5216614924974686E-5
30-31	0.0	3.110049174080211E-4	0.0	0.029419384079137134	2.5216614924974686E-5
32-33	0.0	3.110049174080211E-4	0.0	0.041989866619237017	3.362215323329958E-5
34-35	0.0	3.5723537810380805E-4	0.0	0.0587463072368827	3.362215323329958E-5
36-37	0.0	4.03465838799595E-4	0.0	0.08046201545644006	3.362215323329958E-5
38-39	0.0	4.202769154162448E-4	0.0	0.12623437431442328	3.362215323329958E-5
40-41	0.0	4.6230460695786924E-4	0.0	0.41968852773466203	3.362215323329958E-5
42-43	0.0	4.7071014526619415E-4	0.0	0.46080842113898746	3.362215323329958E-5
44-45	0.0	5.085350676536562E-4	0.0	0.5278215753021076	3.362215323329958E-5
46-47	0.0	5.379544517327933E-4	0.0	0.6056652655755045	3.362215323329958E-5
48-49	0.0	5.463599900411183E-4	0.0	0.6704383437794561	3.362215323329958E-5
50-51	0.0	5.547655283494431E-4	0.0	0.7321560088083316	3.362215323329958E-5
52-53	0.0	5.547655283494431E-4	0.0	0.9444841092457728	3.782492238746203E-5
54-55	0.0	5.589682975036055E-4	0.0	1.6556767055131423	4.202769154162448E-5
56-57	0.0	5.63171066657768E-4	0.0	2.4412583158091867	4.202769154162448E-5
58-59	0.0	5.715766049660929E-4	0.0	3.2558096138848733	4.202769154162448E-5
60-61	0.0	5.757793741202554E-4	0.0	3.871796880503001	4.202769154162448E-5
62-63	0.0	5.883876815827427E-4	0.0	3.8885196989674133	5.883876815827427E-5
64-65	0.0	6.724430646659916E-4	0.0	3.899026621852819	5.883876815827427E-5
66-67	0.0	6.85051372128479E-4	0.0	3.9077137456944735	5.883876815827427E-5
68-69	0.0	7.018624487451288E-4	0.0	3.915312352325199	5.883876815827427E-5
70-71	0.0	7.144707562076161E-4	0.0	3.922301557428571	6.724430646659916E-5
72-73	0.0	7.22876294515941E-4	0.0	3.928097176092161	7.564984477492407E-5
74-75	0.0	7.354846019784284E-4	0.0	3.933552370454264	7.564984477492407E-5
76-77	0.0	7.775122935200528E-4	0.0	3.9386839515914964	7.564984477492407E-5
78-79	0.0	8.0693167759919E-4	0.0	3.9434835139655497	7.564984477492407E-5
80-81	0.0	8.0693167759919E-4	0.0	3.946227922223218	7.564984477492407E-5
82-83	0.0	8.0693167759919E-4	0.0	3.948283076339603	9.246092139157386E-5
84-85	0.0	8.363510616783271E-4	0.0	3.9500776587684308	9.246092139157386E-5
86-87	0.0	8.489593691408144E-4	0.0	3.950443299684843	9.246092139157386E-5
88-89	0.0	8.909870606824389E-4	0.0	3.9504685162997677	1.0086645969989875E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12650	0.0	45.044292	1
GTATCAA	21760	0.0	34.770786	1
TATCTCG	16885	0.0	28.855282	94-95
TCAACGC	26360	0.0	27.988466	4
ATCAACG	26820	0.0	27.60405	3
CAACGCA	27280	0.0	27.044573	5
AACGCAG	28060	0.0	26.308033	6
TATCAAC	28500	0.0	26.157833	2
ACGCAGA	32810	0.0	22.460258	7
CGCAGAG	33030	0.0	22.284777	8
GCTATCT	25285	0.0	21.481068	94-95
CTATCTC	25640	0.0	20.802183	94-95
CCGTCTT	8730	0.0	20.467121	92-93
TATGCCG	8480	0.0	20.273548	88-89
TGCCGTC	8930	0.0	19.34517	90-91
GTACATG	29105	0.0	19.328068	1
GCCGTCT	8765	0.0	19.29658	90-91
CGCTATC	25095	0.0	18.710571	92-93
ATGCCGT	8895	0.0	18.567787	88-89
TACATGG	29680	0.0	18.56475	2
>>END_MODULE