FastQCFastQC Report
Fri 27 May 2016
SRR2139821_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139821_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11175984
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114800.044.6355441
GTATCAA201650.034.877651
TCAACGC243900.027.954144
ATCAACG247050.027.5977123
CAACGCA252800.026.9866665
AACGCAG258650.026.3600036
TATCAAC264100.026.1676392
ACGCAGA300800.022.6522667
CGCAGAG307100.022.1601228
GTACATG271500.019.8823831
TACATGG278250.019.0214482
AGAGTAC328100.018.29513510-11
GCAGAGT373700.018.0867589
ACATGGG285850.018.0288353
GAGTACT208650.017.03515612-13
CAGAGTA360800.016.28079610-11
AGTACTT236000.015.694818512-13
CATGGGG206250.015.3435344
TATACCG8650.015.1016715
GTACTTT237350.015.00262614-15
Produced by FastQC (version 0.11.5)