FastQCFastQC Report
Fri 27 May 2016
SRR2139820_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139820_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11066186
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121950.047.2599261
GTATCAA208050.032.705411
TCAACGC249200.026.5629444
ATCAACG253550.026.1237743
CAACGCA256750.025.7164385
AACGCAG264800.024.934656
TATCAAC273000.024.4477422
ACGCAGA305050.021.6583947
CGCAGAG309100.021.3610328
GTACATG264900.018.0328581
GAGTACT219350.017.6052712-13
AGAGTAC332850.017.6047310-11
GCAGAGT376300.017.3901949
TACATGG269150.017.3270242
CAGAGTA361450.016.84465210-11
ACATGGG270050.016.5693513
AGTACTT249200.015.26906112-13
GTACTTT256100.015.11274314-15
GTGGTAT65700.014.6949041
TGGTATC60450.014.5129032
Produced by FastQC (version 0.11.5)