##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139820_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11066186
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02703207771856	33.0	31.0	34.0	30.0	34.0
2	32.192450858859594	33.0	31.0	34.0	30.0	34.0
3	32.2545245489277	34.0	31.0	34.0	30.0	34.0
4	35.90949483408286	37.0	35.0	37.0	35.0	37.0
5	35.791020140091625	37.0	35.0	37.0	35.0	37.0
6	35.80602747866338	37.0	35.0	37.0	35.0	37.0
7	35.693807333439004	37.0	35.0	37.0	33.0	37.0
8	35.68180717367302	37.0	35.0	37.0	33.0	37.0
9	37.26983244272236	39.0	37.0	39.0	33.0	39.0
10-11	37.19117060747036	39.0	37.0	39.0	33.0	39.0
12-13	37.16005374390056	39.0	37.0	39.0	33.0	39.0
14-15	38.364045073885435	40.0	38.0	41.0	33.0	41.0
16-17	38.36636235826869	40.0	38.0	41.0	33.0	41.0
18-19	38.40765720908722	40.0	38.0	41.0	33.5	41.0
20-21	38.385715959151526	40.0	38.0	41.0	34.0	41.0
22-23	38.30431845662675	40.0	38.0	41.0	33.5	41.0
24-25	38.23746434728297	40.0	38.0	41.0	33.0	41.0
26-27	38.0818212633925	40.0	37.0	41.0	33.0	41.0
28-29	37.98452487596646	40.0	37.0	41.0	33.0	41.0
30-31	37.87222656989107	40.0	37.0	41.0	33.0	41.0
32-33	38.03321533224893	40.0	37.0	41.0	33.0	41.0
34-35	38.01597797214711	40.0	37.0	41.0	33.0	41.0
36-37	37.94282214579363	40.0	37.0	41.0	33.0	41.0
38-39	37.77003358906187	40.0	37.0	41.0	33.0	41.0
40-41	37.793565768965436	40.0	37.0	41.0	33.0	41.0
42-43	37.76614548224238	40.0	37.0	41.0	33.0	41.0
44-45	37.7498642707294	40.0	37.0	41.0	33.0	41.0
46-47	37.77661243564398	40.0	37.0	41.0	33.0	41.0
48-49	37.70054989194995	40.0	37.0	41.0	32.0	41.0
50-51	37.62481238759657	40.0	37.0	41.0	32.0	41.0
52-53	37.490416433126654	39.5	36.0	41.0	32.0	41.0
54-55	37.39805925614401	39.0	36.0	41.0	31.5	41.0
56-57	37.26624790429994	39.0	36.0	41.0	31.0	41.0
58-59	37.131152555022226	39.0	35.5	40.5	31.0	41.0
60-61	36.93636768683037	39.0	35.0	40.0	31.0	41.0
62-63	36.705438775615704	38.0	35.0	40.0	31.0	41.0
64-65	36.50568676821439	38.0	35.0	40.0	31.0	41.0
66-67	36.219270657192055	37.5	35.0	40.0	30.0	41.0
68-69	35.944267150856554	37.0	34.0	40.0	30.0	41.0
70-71	35.660812236365516	36.5	34.0	39.0	30.0	41.0
72-73	35.36227146112853	36.0	34.0	39.0	30.0	40.5
74-75	35.02634074219603	36.0	34.0	38.5	29.0	40.0
76-77	33.96079047573497	35.0	32.5	37.0	28.5	39.0
78-79	34.28055294339054	35.0	33.0	37.0	29.0	39.0
80-81	34.16743904130908	35.0	33.0	37.0	29.0	39.0
82-83	33.94882121486371	35.0	33.0	36.0	29.0	38.5
84-85	33.727751034960434	35.0	33.0	36.0	29.0	37.0
86-87	33.511232439345086	35.0	33.0	35.5	29.0	37.0
88-89	33.29370655262396	35.0	33.0	35.0	29.0	36.5
90-91	33.18219679507739	35.0	33.0	35.0	29.0	36.0
92-93	33.10354661993783	35.0	33.0	35.0	29.0	36.0
94-95	33.0501541191434	34.0	33.0	35.0	29.0	36.0
96-97	32.97354980888077	34.0	33.0	35.0	29.0	35.0
98-99	33.09192813258352	34.0	33.0	35.0	29.0	35.0
100-101	32.62825258852888	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	4.0
19	11.0
20	66.0
21	367.0
22	1872.0
23	6448.0
24	16949.0
25	36199.0
26	65378.0
27	103694.0
28	149197.0
29	199746.0
30	255026.0
31	319577.0
32	398465.0
33	502842.0
34	658001.0
35	895272.0
36	1332633.0
37	2171963.0
38	2625053.0
39	1277309.0
40	50113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.94550353662951	22.318927225694562	15.528240714551517	24.207328523124406
2	13.80353836225971	22.841130829325625	41.0283981508349	22.326932657579768
3	16.766463223985152	27.662345994733506	31.772806876446975	23.79838390483437
4	12.09171795955716	19.70009360045096	39.69016967544193	28.51801876454995
5	12.592233674727678	38.34354492143906	36.43390776189737	12.630313641935894
6	28.52027789881717	39.669638663221455	19.205939607376923	12.60414383058445
7	24.96864773463956	33.19620689549227	24.643377582845616	17.191767787022556
8	23.471600784588293	37.63171882345011	21.91887972965573	16.977800662305874
9	24.967789263618016	16.830965971473823	21.959029063852714	36.24221570105545
10-11	23.33658136597379	27.35176780871025	29.981092853490804	19.33055797182516
12-13	24.2555745945351	24.924730164484853	29.86992537446958	20.94976986651047
14-15	21.480187482841874	26.386954818941234	27.26118104286337	24.871676655353525
16-17	21.151180722969958	29.404995542276264	27.952006228704267	21.49181750604951
18-19	21.20413934846206	28.67656028915473	29.453512709799025	20.66578765258419
20-21	21.934437466576988	27.891144966457816	29.278609702145197	20.895807864820004
22-23	21.66611618917621	27.829192581247476	29.117739204536903	21.386952025039413
24-25	21.521090305821367	27.951801205986833	29.141263212628584	21.385845275563216
26-27	21.521051813796717	28.06588343057913	29.0279601934075	21.385104562216657
28-29	21.50909713125835	28.087591830027627	29.075453454025173	21.327857584688847
30-31	21.71112120288967	27.979316132261694	28.98666941485175	21.322893249996884
32-33	21.520444286293213	28.130422636164308	28.937725045361006	21.411408032181477
34-35	21.639686168263797	28.154462104210058	28.90141670055688	21.30443502696926
36-37	21.625060904220653	28.095131304574707	28.88269073987833	21.39711705132631
38-39	21.556002059039482	28.1982080599522	28.898671740550324	21.34711814045799
40-41	21.725636239238334	28.060287456104366	28.814326746517853	21.399749558139444
42-43	21.61761888839354	28.17501190395195	28.628890897242616	21.578478310411896
44-45	21.731706075493072	28.238887785768092	28.464130820663108	21.56527531807573
46-47	21.766010258855843	28.21202559242635	28.281953440209755	21.740010708508056
48-49	21.713467242719823	28.307355032125415	28.239423658363823	21.739754066790944
50-51	21.7139759377993	28.402197559856674	28.143462016560072	21.740364485783957
52-53	21.869588888792105	28.295360420207228	28.069611044435845	21.765439646564825
54-55	21.856803100438515	28.24950054373703	28.095970703466676	21.797725652357776
56-57	21.84454866647844	28.313448854581956	28.07294213034035	21.76906034859925
58-59	21.834666238735746	28.35172006014431	28.01463823996233	21.79897546115761
60-61	21.867010065283296	28.334676474940956	28.08068507347363	21.717628386302117
62-63	21.884160656818217	28.372895888265976	28.021644674960534	21.721298779955266
64-65	21.87144969743689	28.308302320880514	27.997736390155726	21.822511591526872
66-67	21.880717788159053	28.341125643417413	27.9231220140991	21.855034554324433
68-69	21.85160201059986	28.301992621286242	27.975415858898867	21.870989509215036
70-71	21.85582609984005	28.146375700712177	28.033818137999095	21.963980061448673
72-73	21.896082069294355	28.133144011568344	27.99911245816043	21.97166146097687
74-75	21.79164277523334	28.243749231589838	27.954763120778882	22.00984487239794
76-77	21.899038111304705	28.18670105946647	27.90525223933568	22.009008589893146
78-79	21.84535744700519	28.201241135711726	27.88485063634008	22.068550780943006
80-81	21.891806156361802	28.170646999436933	27.911599180483293	22.025947663717975
82-83	21.879301229986204	28.150202965892586	27.976659215401728	21.993836588719482
84-85	21.922830369640035	28.15161084877727	27.964605998168103	21.96095278341459
86-87	21.855445591827966	28.13999681209417	27.994563680106914	22.00999391597095
88-89	21.914759198947774	28.044961575033188	28.00163530044052	22.03864392557852
90-91	21.91709899726863	28.053963426764394	28.036622673871868	21.992314902095107
92-93	21.948412970683545	27.98149092523687	28.04360974517206	22.026486358907526
94-95	21.796681117867077	27.999040179856326	28.128706448322678	22.075572253953922
96-97	21.77969349790174	28.02773009965886	28.190374941641256	22.002201460798148
98-99	21.97146934888928	28.649165304450474	28.250512804462936	21.12885254219731
100-101	22.513970652402055	31.32579554407816	25.486100528706228	20.674133274813553
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2535.0
1	2194.5
2	2800.5
3	3961.5
4	5150.5
5	7790.0
6	10174.0
7	12160.0
8	13497.5
9	13483.0
10	12712.0
11	12000.5
12	13297.0
13	17520.0
14	27216.0
15	40630.0
16	51229.5
17	56881.0
18	58664.5
19	57764.0
20	55841.5
21	54944.5
22	57297.5
23	65020.5
24	79118.5
25	99708.0
26	124903.5
27	150192.0
28	175618.0
29	202116.5
30	226934.0
31	252420.0
32	278750.0
33	304452.5
34	330225.0
35	353115.5
36	377264.5
37	399146.5
38	415413.5
39	429507.0
40	441099.5
41	451972.5
42	459511.0
43	464145.5
44	466791.0
45	467190.0
46	465143.0
47	459178.0
48	451069.0
49	440590.5
50	427112.5
51	408737.5
52	385710.0
53	360624.5
54	334752.0
55	308579.5
56	281925.0
57	254223.0
58	224305.5
59	192701.5
60	160807.5
61	130527.5
62	103001.0
63	79221.5
64	59115.0
65	43997.0
66	33110.0
67	25200.5
68	19398.0
69	14200.5
70	10220.0
71	7513.5
72	5317.5
73	3581.0
74	2454.0
75	1742.5
76	1190.5
77	774.0
78	504.5
79	340.0
80	232.0
81	169.0
82	122.0
83	84.5
84	67.5
85	56.0
86	41.5
87	31.0
88	24.5
89	16.0
90	9.5
91	7.5
92	6.0
93	3.5
94	1.0
95	0.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0019790016180823273
3	5.421922241321446E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0022953612060369988
22-23	4.383315024331917E-4
24-25	0.0011796850638627707
26-27	0.011566139371889
28-29	3.438467804268659E-4
30-31	0.0025729598319748515
32-33	0.0027245833158505905
34-35	0.0017449776649693
36-37	0.0643229352905502
38-39	0.029011693220367122
40-41	0.026094048861234245
42-43	0.003389977008557866
44-45	0.0016542599533394709
46-47	0.0035263722712842965
48-49	0.002999013078401446
50-51	7.168894372144817E-4
52-53	9.89169333979E-4
54-55	0.0013460377441897487
56-57	0.011772021407616629
58-59	0.008956127350248073
60-61	0.004638967366889317
62-63	0.002626234194097562
64-65	0.0011200681671392936
66-67	5.505032181677072E-4
68-69	0.0017249701210532602
70-71	4.880836280319009E-5
72-73	6.971612308310167E-4
74-75	3.2777985371685133E-4
76-77	0.005456317146802887
78-79	0.00153303083044679
80-81	5.548021740154356E-4
82-83	4.571546996265046E-4
84-85	0.002513698468141457
86-87	0.0030624178633004186
88-89	0.03969080477376976
90-91	3.050966963507073E-4
92-93	4.248614999069426E-4
94-95	1.8756557923258382E-4
96-97	0.018168602240807543
98-99	2.700421286493165E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	226.0
20-21	895.0
22-23	1844.0
24-25	3841.0
26-27	5806.0
28-29	10044.0
30-31	25151.0
32-33	33422.0
34-35	47833.0
36-37	75045.0
38-39	79287.0
40-41	82021.0
42-43	95033.0
44-45	112817.0
46-47	113206.0
48-49	103224.0
50-51	93860.0
52-53	91717.0
54-55	100482.0
56-57	105007.0
58-59	104948.0
60-61	103459.0
62-63	98373.0
64-65	105007.0
66-67	111824.0
68-69	113858.0
70-71	112557.0
72-73	108094.0
74-75	110661.0
76-77	113358.0
78-79	120951.0
80-81	121711.0
82-83	117647.0
84-85	118244.0
86-87	124957.0
88-89	134307.0
90-91	143992.0
92-93	149405.0
94-95	183290.0
96-97	237228.0
98-99	887715.0
100-101	6463839.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.20420292947325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.06464263826558	79.41219576327273
2	6.40990399647902	11.17941137734796
3	1.4313205644276443	3.7445150687242945
4	0.517142342443589	1.8038794309549557
5	0.23820358043111214	1.0386176683220902
6	0.11814784417672967	0.6181793147560392
7	0.0642371339564732	0.3921223645603632
8	0.03937474854113472	0.27469148496624746
9	0.02706534513122623	0.21241906642617145
>10	0.08828318671376538	1.188640571329623
>50	0.0014560526409665285	0.08363079567940541
>100	1.9110910816993662E-4	0.035512099411277497
>500	3.145768453345422E-5	0.01618499424884727
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.036537068869076E-6
2	0.0	3.6146148275476305E-5	0.0	0.0	9.036537068869076E-6
3	0.0	3.6146148275476305E-5	0.0	0.0	9.036537068869076E-6
4	0.0	6.325575948208352E-5	0.0	9.036537068869076E-6	9.036537068869076E-6
5	0.0	6.325575948208352E-5	0.0	9.036537068869076E-6	9.036537068869076E-6
6	2.710961120660723E-5	6.325575948208352E-5	0.0	1.8073074137738152E-5	9.036537068869076E-6
7	2.710961120660723E-5	7.229229655095261E-5	0.0	2.710961120660723E-5	9.036537068869076E-6
8	2.710961120660723E-5	7.229229655095261E-5	0.0	2.710961120660723E-5	9.036537068869076E-6
9	2.710961120660723E-5	1.5362113017077428E-4	0.0	7.229229655095261E-5	2.710961120660723E-5
10-11	5.421922241321446E-5	1.5362113017077428E-4	0.0	1.0392017629199437E-4	2.710961120660723E-5
12-13	6.325575948208352E-5	1.5362113017077428E-4	0.0	1.1747498189529799E-4	2.710961120660723E-5
14-15	6.777402801651807E-5	2.485047693938996E-4	0.0	1.310297874986016E-4	2.710961120660723E-5
16-17	9.940190775755983E-5	2.7109611206607226E-4	0.0	1.6265766723964336E-4	2.710961120660723E-5
18-19	1.0843844482642891E-4	2.846509176693759E-4	0.0	1.807307413773815E-4	2.710961120660723E-5
20-21	1.0843844482642891E-4	2.982057232726795E-4	0.0	2.168768896528578E-4	2.710961120660723E-5
22-23	1.0843844482642891E-4	3.433884086170249E-4	0.0	2.394682323250305E-4	3.162787974104177E-5
24-25	1.0843844482642891E-4	3.659797512891976E-4	0.0	2.7109611206607226E-4	3.6146148275476305E-5
26-27	1.0843844482642891E-4	3.795345568925012E-4	0.0	3.343518715481558E-4	3.6146148275476305E-5
28-29	1.0843844482642891E-4	3.930893624958048E-4	0.0	7.72623919388306E-4	3.6146148275476305E-5
30-31	1.0843844482642891E-4	4.6086339051232283E-4	0.0	0.0019383372012724167	3.6146148275476305E-5
32-33	1.1747498189529799E-4	4.7441819611562645E-4	0.0	0.004360129135729329	3.6146148275476305E-5
34-35	1.1747498189529799E-4	5.557470297354481E-4	0.0	0.00772623919388306	5.421922241321446E-5
36-37	1.1747498189529799E-4	6.280393262864007E-4	0.0	0.012691816313226616	5.421922241321446E-5
38-39	1.1747498189529799E-4	6.506306689585735E-4	0.0	0.019997856533407264	5.421922241321446E-5
40-41	1.1747498189529799E-4	7.138864284406569E-4	0.0	0.027724095727290322	6.325575948208352E-5
42-43	1.1747498189529799E-4	7.500325767161333E-4	0.0	0.03479066771514594	6.777402801651807E-5
44-45	1.2651151896416705E-4	8.675075586114312E-4	0.0	0.04400341725685796	7.229229655095261E-5
46-47	1.3554805603303613E-4	9.533546607656874E-4	0.0	0.05395716283821725	7.229229655095261E-5
48-49	1.3554805603303613E-4	9.57872929300122E-4	0.0	0.06350426425147743	8.132883361982168E-5
50-51	1.3554805603303613E-4	0.0010120921517133364	0.0	0.07307847527594422	8.584710215425622E-5
52-53	1.3554805603303613E-4	0.0010211286887822056	0.0	0.08195235467757364	9.940190775755984E-5
54-55	1.3554805603303613E-4	0.00102564695731664	0.0	0.09143168206281731	1.1295671336086346E-4
56-57	1.3554805603303613E-4	0.0010301652258510746	0.0	0.09997572786143302	1.1747498189529799E-4
58-59	1.3554805603303613E-4	0.0010301652258510746	0.0	0.10768841224971278	1.1747498189529799E-4
60-61	1.4458459310190522E-4	0.0010392017629199438	0.0	0.11462847271860421	1.1747498189529799E-4
62-63	1.4458459310190522E-4	0.0010934209853331583	0.0	0.12164082548404662	1.2651151896416705E-4
64-65	1.4458459310190522E-4	0.0011747498189529798	0.0	0.12873450708310885	1.310297874986016E-4
66-67	1.4458459310190522E-4	0.0011792680874874143	0.0	0.1360947665257027	1.3554805603303613E-4
68-69	1.4458459310190522E-4	0.0011883046245562835	0.0	0.14421409508208158	1.3554805603303613E-4
70-71	1.5362113017077428E-4	0.0011973411616251525	0.0	0.1514478430057113	1.3554805603303613E-4
72-73	1.897672784462506E-4	0.0012108959672284562	0.0	0.1575836516754734	1.3554805603303613E-4
74-75	1.9880381551511965E-4	0.0012289690413661942	0.0	0.16309142101894908	1.3554805603303613E-4
76-77	1.9880381551511965E-4	0.0012651151896416707	0.0	0.16830550290768653	1.3554805603303613E-4
78-79	1.9880381551511965E-4	0.0012967430693827124	0.0	0.17351506652788956	1.3554805603303613E-4
80-81	1.9880381551511965E-4	0.0013057796064515814	0.0	0.1765423064459607	1.4458459310190522E-4
82-83	1.9880381551511965E-4	0.0013148161435204506	0.0	0.17856649074938735	1.5362113017077428E-4
84-85	1.9880381551511965E-4	0.0013464440232614922	0.0	0.18031957894074796	1.5362113017077428E-4
86-87	2.0332208404955418E-4	0.0013599988288647959	0.0	0.18057712024721073	1.5813939870520883E-4
88-89	2.0784035258398874E-4	0.0014096997827435758	0.0	0.18061326639548622	1.6265766723964336E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12195	0.0	47.259926	1
GTATCAA	20805	0.0	32.70541	1
TCAACGC	24920	0.0	26.562944	4
ATCAACG	25355	0.0	26.123774	3
CAACGCA	25675	0.0	25.716438	5
AACGCAG	26480	0.0	24.93465	6
TATCAAC	27300	0.0	24.447742	2
ACGCAGA	30505	0.0	21.658394	7
CGCAGAG	30910	0.0	21.361032	8
GTACATG	26490	0.0	18.032858	1
GAGTACT	21935	0.0	17.60527	12-13
AGAGTAC	33285	0.0	17.60473	10-11
GCAGAGT	37630	0.0	17.390194	9
TACATGG	26915	0.0	17.327024	2
CAGAGTA	36145	0.0	16.844652	10-11
ACATGGG	27005	0.0	16.569351	3
AGTACTT	24920	0.0	15.269061	12-13
GTACTTT	25610	0.0	15.112743	14-15
GTGGTAT	6570	0.0	14.694904	1
TGGTATC	6045	0.0	14.512903	2
>>END_MODULE