FastQCFastQC Report
Fri 27 May 2016
SRR2139819_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139819_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10785791
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132100.050.8318021
GTATCAA240500.036.5141031
TCAACGC309050.027.9947784
ATCAACG313550.027.633033
CAACGCA316150.027.379315
TATCAAC325300.026.9054872
AACGCAG324500.026.7908236
ACGCAGA377650.023.009247
CGCAGAG385000.022.5808378
GTACATG349150.019.3519061
GCAGAGT446700.019.2183749
AGAGTAC403400.018.968510-11
TACATGG355450.018.7499682
GAGTACT233850.018.58803212-13
ACATGGG356900.018.1459473
CAGAGTA433850.017.47325310-11
AGTACTT264550.016.61282512-13
GTACTTT266600.016.32940714-15
CATGGGG264200.016.1043574
GTGGTAT65450.016.0445421
Produced by FastQC (version 0.11.5)