##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139819_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10785791
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00994984976067	33.0	31.0	34.0	30.0	34.0
2	32.17025380892324	33.0	31.0	34.0	30.0	34.0
3	32.22588867149382	34.0	31.0	34.0	30.0	34.0
4	35.89045689833968	37.0	35.0	37.0	35.0	37.0
5	35.767061683283124	37.0	35.0	37.0	35.0	37.0
6	35.7850487738915	37.0	35.0	37.0	33.0	37.0
7	35.6724172571117	37.0	35.0	37.0	33.0	37.0
8	35.65921006628072	37.0	35.0	37.0	33.0	37.0
9	37.23900157160472	39.0	37.0	39.0	33.0	39.0
10-11	37.15724206041077	39.0	37.0	39.0	33.0	39.0
12-13	37.11991216035987	39.0	37.0	39.0	33.0	39.0
14-15	38.29836476527312	40.0	38.0	41.0	33.0	41.0
16-17	38.29903161483474	40.0	38.0	41.0	33.0	41.0
18-19	38.34294188530076	40.0	38.0	41.0	33.5	41.0
20-21	38.31929897917725	40.0	38.0	41.0	33.5	41.0
22-23	38.23880640375391	40.0	38.0	41.0	33.0	41.0
24-25	38.16028813066664	40.0	37.5	41.0	33.0	41.0
26-27	37.9890891427964	40.0	37.0	41.0	33.0	41.0
28-29	37.88506687737235	40.0	37.0	41.0	33.0	41.0
30-31	37.76406655164209	40.0	37.0	41.0	32.5	41.0
32-33	37.933650918906224	40.0	37.0	41.0	33.0	41.0
34-35	37.92277465179401	40.0	37.0	41.0	33.0	41.0
36-37	37.84630491302771	40.0	37.0	41.0	33.0	41.0
38-39	37.65959087964069	40.0	37.0	41.0	32.0	41.0
40-41	37.68213120425067	40.0	37.0	41.0	33.0	41.0
42-43	37.65107128579	40.0	37.0	41.0	32.5	41.0
44-45	37.62816336679414	40.0	37.0	41.0	32.5	41.0
46-47	37.648887027668245	40.0	37.0	41.0	32.0	41.0
48-49	37.56868645397111	40.0	36.5	41.0	32.0	41.0
50-51	37.48314239533866	39.5	36.0	41.0	32.0	41.0
52-53	37.34249052242673	39.0	36.0	41.0	31.0	41.0
54-55	37.23496302715476	39.0	36.0	41.0	31.0	41.0
56-57	37.089867785053755	39.0	35.0	40.5	31.0	41.0
58-59	36.93853156971477	39.0	35.0	40.0	31.0	41.0
60-61	36.729639243549784	38.5	35.0	40.0	31.0	41.0
62-63	36.48276837797757	38.0	35.0	40.0	31.0	41.0
64-65	36.266808163857	38.0	35.0	40.0	30.0	41.0
66-67	35.97152490324201	37.0	34.0	40.0	30.0	41.0
68-69	35.68267162673361	37.0	34.0	39.0	30.0	41.0
70-71	35.39112381079775	36.0	34.0	39.0	29.0	40.5
72-73	35.09128334993561	36.0	34.0	39.0	29.0	40.0
74-75	34.758941692177444	35.0	34.0	38.0	29.0	40.0
76-77	33.717432922787836	35.0	32.5	37.0	28.0	39.0
78-79	34.0558825235394	35.0	33.0	37.0	29.0	39.0
80-81	33.96166581013932	35.0	33.0	36.0	29.0	39.0
82-83	33.76096656636661	35.0	33.0	36.0	29.0	37.5
84-85	33.55659475515425	35.0	33.0	36.0	29.0	37.0
86-87	33.36704046912382	35.0	33.0	35.0	29.0	37.0
88-89	33.16549010618806	35.0	33.0	35.0	29.0	36.0
90-91	33.07166576969703	35.0	33.0	35.0	29.0	36.0
92-93	33.008148068588106	34.5	33.0	35.0	29.0	36.0
94-95	32.96566346293977	34.0	33.0	35.0	29.0	35.5
96-97	32.89362240592437	34.0	32.0	35.0	29.0	35.0
98-99	33.02203001052143	34.0	32.5	35.0	29.0	35.0
100-101	32.561796794039	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	1.0
15	0.0
16	0.0
17	1.0
18	3.0
19	20.0
20	99.0
21	460.0
22	2127.0
23	7119.0
24	18505.0
25	38689.0
26	69183.0
27	107795.0
28	154505.0
29	204217.0
30	260128.0
31	322787.0
32	399986.0
33	506429.0
34	666236.0
35	910430.0
36	1367207.0
37	2186814.0
38	2416827.0
39	1104816.0
40	41405.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.281179377571846	21.998896511159913	15.592829492060433	24.12709461920781
2	14.137704646875632	22.674686996753355	40.43013498286742	22.757473373503593
3	16.931164638177734	27.624689880999785	31.588492588249768	23.85565289257271
4	12.440710189915603	19.384011798485616	38.858513019582894	29.316764992015887
5	12.838529876946438	38.34329814104501	35.85894627477948	12.959225707229075
6	29.090216934483525	38.97061420900887	18.862955901889812	13.076212954617795
7	25.456232185474388	33.11853530260321	24.064530825787372	17.360701686135027
8	24.268855200327913	36.900112379333144	21.485044536835545	17.345987883503398
9	25.56076786579677	16.464040514042967	21.389798856662438	36.58539276349783
10-11	23.922357664820318	26.993588138320128	29.317886838341295	19.766167358518256
12-13	24.799520962347593	24.648164422989467	29.303372372040215	21.248942242622725
14-15	22.07362445647241	25.915887856532727	26.77704861887274	25.233439068122127
16-17	21.58937624509876	28.938053778345974	27.45185309079325	22.020716885762017
18-19	21.724679256254824	28.14991501318726	29.047132472713404	21.07827325784451
20-21	22.49644370408132	27.280308440405456	28.859232433492956	21.364015422020273
22-23	22.279801586569327	27.17823631702418	28.76143959064364	21.780522505762857
24-25	22.082592963368022	27.451666135031793	28.665143213530136	21.80059768807005
26-27	21.96928300278638	27.54556451103972	28.677679783860675	21.80747270231322
28-29	21.902469414996535	27.497813392027297	28.732945699742174	21.86677149323399
30-31	22.19846832953524	27.39948758353128	28.628649249490586	21.773394837442897
32-33	22.011936480472237	27.635460035537225	28.517336982766494	21.83526650122404
34-35	22.08713804739003	27.64349240709887	28.572700169042964	21.696669376468133
36-37	22.08595831428946	27.618246215106907	28.501855213385763	21.79394025721787
38-39	22.069037204733345	27.62903363813358	28.524942841069606	21.776986316063468
40-41	22.167787353654667	27.509160489328455	28.460245214257856	21.862806942759022
42-43	22.04612369928698	27.619785055371597	28.252707840466723	22.081383404874696
44-45	22.102008574911096	27.72945863370206	28.098822669148987	22.069710122237858
46-47	22.245917774036965	27.64903294779209	27.858590671942697	22.24645860622824
48-49	22.151152816268898	27.810421383307936	27.78908945891233	22.249336341510837
50-51	22.16556696495385	27.904011056949113	27.669261822086376	22.26116015601066
52-53	22.30523845334673	27.765781919142935	27.610098814082974	22.318880813427366
54-55	22.27918977785673	27.69616562154215	27.65186112410597	22.37278347649515
56-57	22.250579069626237	27.79391979219797	27.594678258851186	22.36082287932461
58-59	22.267227538882985	27.837206661453756	27.60247413116496	22.293091668498295
60-61	22.390599506642197	27.813581268579217	27.56582399025654	22.229995234522043
62-63	22.36796268987029	27.91093828877998	27.516979468067955	22.204119553281775
64-65	22.320273135347907	27.819676268868566	27.531389300240015	22.32866129554351
66-67	22.39524813866087	27.819909517394407	27.39827701202599	22.386565331918728
68-69	22.288391950584106	27.77787519737841	27.52071695417281	22.41301589786468
70-71	22.321908225160406	27.587282135983855	27.59006033680868	22.50074930204706
72-73	22.35819811619754	27.712540329194674	27.412389709393853	22.516871845213938
74-75	22.193689971789933	27.78837800453267	27.4703444062479	22.54758761742949
76-77	22.345311172786428	27.63987477224299	27.46446124204177	22.55035281292881
78-79	22.311574328224644	27.627592876732766	27.41105888304637	22.649773911996224
80-81	22.390991209704524	27.646049154341146	27.388992912694654	22.57396672325968
82-83	22.38908264104688	27.59549593886436	27.506653765260825	22.508767654827935
84-85	22.382553263791433	27.609344808983	27.450288757605897	22.557813169619674
86-87	22.24598795664682	27.659294054099053	27.521377772483252	22.57334021677087
88-89	22.365186422265847	27.55925665098496	27.483557038361255	22.59199988838794
90-91	22.40112360420036	27.53798870721031	27.517849726966244	22.543037961623085
92-93	22.47341210988227	27.456125521766133	27.508700455007727	22.56176191334387
94-95	22.23963357935237	27.470158264275263	27.606500051286552	22.683708105085817
96-97	22.248704392992387	27.46739834241839	27.675196347510756	22.608700917078465
98-99	22.436364935054762	28.07703647621616	27.741389493284363	21.74520909544471
100-101	23.052765873924564	30.794813918239612	24.82494773006948	21.32747247776635
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3620.0
1	3075.0
2	3553.0
3	4834.0
4	6274.0
5	9296.0
6	11871.0
7	14178.0
8	15995.0
9	16318.5
10	15269.5
11	14098.0
12	15432.5
13	20106.0
14	31165.0
15	46118.0
16	57633.0
17	63075.5
18	63554.5
19	61028.5
20	56955.5
21	53274.0
22	52880.0
23	58280.0
24	69696.5
25	87056.0
26	108439.5
27	130124.5
28	152007.5
29	174060.0
30	195083.0
31	217237.0
32	241741.0
33	266476.0
34	288925.0
35	311158.5
36	337166.5
37	357658.0
38	372533.0
39	386979.5
40	399279.5
41	410432.5
42	417879.0
43	427182.5
44	438876.5
45	446118.5
46	449048.5
47	449220.0
48	447317.5
49	443788.0
50	437258.5
51	426540.0
52	411003.0
53	389577.0
54	366679.0
55	343827.5
56	318880.0
57	292460.0
58	262756.0
59	227660.5
60	190750.5
61	155588.5
62	123075.5
63	95054.5
64	71193.0
65	53340.0
66	40519.0
67	30702.0
68	23629.5
69	17310.0
70	12165.0
71	8796.0
72	6347.0
73	4433.5
74	3006.5
75	2030.5
76	1383.0
77	915.5
78	631.5
79	447.0
80	321.0
81	228.0
82	174.0
83	126.5
84	92.5
85	70.5
86	53.0
87	40.5
88	26.0
89	16.0
90	11.5
91	8.5
92	8.0
93	5.5
94	2.5
95	2.5
96	3.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001854291446960172
3	1.1125748681761031E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0023504101836643895
22-23	4.54368512150834E-4
24-25	0.0011594112565291087
26-27	0.01271267988674023
28-29	3.4820325264550906E-4
30-31	0.0026731712904936636
32-33	0.002880557810231648
34-35	0.0018007850019399366
36-37	0.062497573012481746
38-39	0.02597049386235714
40-41	0.024950448752460858
42-43	0.003547021741029392
44-45	0.001876142788464366
46-47	0.00391350524109798
48-49	0.003256107089832664
50-51	8.638166876527248E-4
52-53	0.0011203745072316626
54-55	0.0017348924643026684
56-57	0.012307561196762427
58-59	0.009368137917391843
60-61	0.005312313837758235
62-63	0.002912230813689045
64-65	0.0015339349346929901
66-67	5.409648944929938E-4
68-69	0.0013663142792786215
70-71	4.480967064387968E-5
72-73	4.6508383136060273E-4
74-75	1.5503166464331204E-4
76-77	0.004605374367218945
78-79	0.0011960782303509978
80-81	6.454753522547261E-4
82-83	4.304761599877381E-4
84-85	0.0027118428946395537
86-87	0.003263556457841902
88-89	0.041773649574862785
90-91	3.869145499216498E-4
92-93	5.256151652064311E-4
94-95	1.7430694218962772E-4
96-97	0.017520984939382346
98-99	2.0839114990092795E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	277.0
20-21	928.0
22-23	1982.0
24-25	4565.0
26-27	6181.0
28-29	10924.0
30-31	26177.0
32-33	34185.0
34-35	48409.0
36-37	75513.0
38-39	81621.0
40-41	83583.0
42-43	96830.0
44-45	115183.0
46-47	114647.0
48-49	104773.0
50-51	94892.0
52-53	90732.0
54-55	99401.0
56-57	104302.0
58-59	105274.0
60-61	103568.0
62-63	99164.0
64-65	104288.0
66-67	111648.0
68-69	111792.0
70-71	112279.0
72-73	107756.0
74-75	109086.0
76-77	110515.0
78-79	119010.0
80-81	120371.0
82-83	116218.0
84-85	116863.0
86-87	122758.0
88-89	131554.0
90-91	142404.0
92-93	147032.0
94-95	180321.0
96-97	232266.0
98-99	867073.0
100-101	6219446.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.19382315238904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.11854389054808	76.77543290962164
2	6.9191345257078885	11.78935046301494
3	1.6457181107268837	4.206150530518498
4	0.5921229846530043	2.017808833559713
5	0.2650870021201434	1.1291887589310237
6	0.14629185991124605	0.747789770514766
7	0.08606533959072292	0.5132564724449649
8	0.05558194568475644	0.37881907609062665
9	0.036279179462477475	0.27816857993160815
>10	0.1301464332865292	1.7868254460642237
>50	0.003938767840816891	0.22483441937994575
>100	0.0010712923763519684	0.14332532945036883
>500	1.8668091188965343E-5	0.009049410477675246
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.854291446960172E-5	0.0	0.0	0.0
3	0.0	1.854291446960172E-5	0.0	0.0	0.0
4	0.0	7.417165787840688E-5	0.0	9.27145723480086E-6	9.27145723480086E-6
5	9.27145723480086E-6	8.344311511320774E-5	0.0	1.854291446960172E-5	9.27145723480086E-6
6	6.490020064360602E-5	8.344311511320774E-5	0.0	2.781437170440258E-5	9.27145723480086E-6
7	6.490020064360602E-5	8.344311511320774E-5	0.0	2.781437170440258E-5	9.27145723480086E-6
8	6.490020064360602E-5	8.344311511320774E-5	0.0	2.781437170440258E-5	1.854291446960172E-5
9	6.490020064360602E-5	1.4834331575681376E-4	0.0	4.63572861740043E-5	2.781437170440258E-5
10-11	7.417165787840688E-5	1.4834331575681376E-4	0.0	6.026447202620559E-5	2.781437170440258E-5
12-13	8.344311511320774E-5	1.5297904437421418E-4	0.0	7.417165787840688E-5	2.781437170440258E-5
14-15	8.807884373060817E-5	1.9470060193081806E-4	0.0	8.807884373060817E-5	5.099301479140473E-5
16-17	1.4834331575681376E-4	1.9933633054821848E-4	0.0	1.2052894405241118E-4	5.562874340880516E-5
18-19	1.576147729916146E-4	2.4105788810482236E-4	0.0	1.3443612990461247E-4	5.562874340880516E-5
20-21	1.576147729916146E-4	2.827794456614262E-4	0.0	1.4370758713941334E-4	5.562874340880516E-5
22-23	1.6688623022641547E-4	3.1059381736582884E-4	0.0	1.4834331575681376E-4	7.417165787840688E-5
24-25	2.0397205916561893E-4	3.245010032180301E-4	0.0	1.7615768746121634E-4	1.0198602958280945E-4
26-27	2.178792450178202E-4	3.245010032180301E-4	0.0	2.549650739570237E-4	1.1125748681761032E-4
28-29	2.3178643087002149E-4	3.245010032180301E-4	0.0	6.675449209056619E-4	1.1125748681761032E-4
30-31	2.4105788810482236E-4	3.523153749224327E-4	0.0	0.0018357485324905702	1.1125748681761032E-4
32-33	2.503293453396232E-4	3.569511035398331E-4	0.0	0.004352949171739003	1.2052894405241118E-4
34-35	2.874151742788267E-4	4.172155755660387E-4	0.0	0.0075840520180671025	1.390718585220129E-4
36-37	3.0595808874842837E-4	4.7748004759224423E-4	0.0	0.01240984550878095	1.390718585220129E-4
38-39	3.105938173658288E-4	4.913872334444456E-4	0.0	0.019660125066395223	1.390718585220129E-4
40-41	3.152295459832292E-4	5.377445196184499E-4	0.0	0.02784218607610698	1.390718585220129E-4
42-43	3.245010032180301E-4	5.423802482358504E-4	0.0	0.03569974608260071	1.390718585220129E-4
44-45	3.3840818907023137E-4	5.841018057924541E-4	0.0	0.044248029653087106	1.390718585220129E-4
46-47	3.523153749224327E-4	6.25823363349058E-4	0.0	0.05459497592712487	1.4370758713941334E-4
48-49	3.523153749224327E-4	6.304590919664584E-4	0.0	0.06480748607125801	1.6688623022641547E-4
50-51	3.847654752442357E-4	6.675449209056619E-4	0.0	0.07465840938323393	1.6688623022641547E-4
52-53	3.9403693247903654E-4	6.675449209056619E-4	0.0	0.08437953229392263	1.900648733134176E-4
54-55	4.0794411833123786E-4	6.675449209056619E-4	0.0	0.09460131389529058	2.2251497363522061E-4
56-57	4.172155755660387E-4	6.675449209056619E-4	0.0	0.1045449517796145	2.3642215948742193E-4
58-59	4.2648703280083954E-4	6.768163781404627E-4	0.0	0.11353826529737132	2.456936167222228E-4
60-61	4.3112276141823996E-4	6.860878353752636E-4	0.0	0.1218872125373095	2.596008025744241E-4
62-63	4.450299472704413E-4	7.185379356970667E-4	0.0	0.13007854500425609	2.6887225980922494E-4
64-65	4.450299472704413E-4	8.066167794276748E-4	0.0	0.13871490741847306	2.6887225980922494E-4
66-67	4.5893713312264255E-4	8.29795422514677E-4	0.0	0.14724464807448984	2.6887225980922494E-4
68-69	4.6357286174004297E-4	8.715169800712808E-4	0.0	0.1564975623948211	2.7814371704402584E-4
70-71	4.7748004759224423E-4	8.807884373060817E-4	0.0	0.16467962340453285	2.874151742788267E-4
72-73	5.052944192966468E-4	8.807884373060817E-4	0.0	0.17072461352162305	3.3377246045283095E-4
74-75	5.099301479140472E-4	9.132385376278847E-4	0.0	0.17617159464706855	3.3377246045283095E-4
76-77	5.099301479140472E-4	0.0010013173813584928	0.0	0.18169274743039246	3.3377246045283095E-4
78-79	5.192016051488482E-4	0.0010384032102976964	0.0	0.18675032735197633	3.3840818907023137E-4
80-81	5.192016051488482E-4	0.0010384032102976964	0.0	0.1896801078381734	3.6622256077463395E-4
82-83	5.192016051488482E-4	0.0010430389389150968	0.0	0.1918125430021776	4.2648703280083954E-4
84-85	5.192016051488482E-4	0.0010476746675324971	0.0	0.19359266279125936	4.2648703280083954E-4
86-87	5.192016051488482E-4	0.0010615818533846984	0.0	0.19382908495074677	4.4039421865304086E-4
88-89	5.192016051488482E-4	0.0010940319537065014	0.0	0.193866170779686	4.450299472704413E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13210	0.0	50.831802	1
GTATCAA	24050	0.0	36.514103	1
TCAACGC	30905	0.0	27.994778	4
ATCAACG	31355	0.0	27.63303	3
CAACGCA	31615	0.0	27.37931	5
TATCAAC	32530	0.0	26.905487	2
AACGCAG	32450	0.0	26.790823	6
ACGCAGA	37765	0.0	23.00924	7
CGCAGAG	38500	0.0	22.580837	8
GTACATG	34915	0.0	19.351906	1
GCAGAGT	44670	0.0	19.218374	9
AGAGTAC	40340	0.0	18.9685	10-11
TACATGG	35545	0.0	18.749968	2
GAGTACT	23385	0.0	18.588032	12-13
ACATGGG	35690	0.0	18.145947	3
CAGAGTA	43385	0.0	17.473253	10-11
AGTACTT	26455	0.0	16.612825	12-13
GTACTTT	26660	0.0	16.329407	14-15
CATGGGG	26420	0.0	16.104357	4
GTGGTAT	6545	0.0	16.044542	1
>>END_MODULE