FastQCFastQC Report
Fri 27 May 2016
SRR2139818_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139818_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7822071
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84450.045.3208121
GTATCAA150050.034.3925671
TCAACGC178850.028.007074
ATCAACG179350.027.9758913
CAACGCA182800.027.4018865
AACGCAG188300.026.7801956
TATCAAC195600.026.0824222
ACGCAGA218250.022.989587
CGCAGAG220100.022.7772398
GTACATG189800.019.3230761
TACATGG188500.019.1885912
GCAGAGT269400.018.4216849
ACATGGG191200.018.2792343
AGAGTAC238450.018.1317910-11
GAGTACT157700.017.89516412-13
CAGAGTA263400.016.54203210-11
CTTATAC58100.016.504971
AGTACTT175500.015.93636912-13
GTACTTT179800.015.69664614-15
CATGGGG138550.015.3295614
Produced by FastQC (version 0.11.5)