FastQCFastQC Report
Fri 27 May 2016
SRR2139817_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139817_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18987921
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203250.047.668811
GTATCAA356800.034.6521531
TCAACGC436000.027.6339534
ATCAACG444650.027.0963763
CAACGCA449000.026.8712755
AACGCAG464000.026.0749976
TATCAAC475100.025.5641022
ACGCAGA537600.022.5364517
CGCAGAG546950.022.1511948
GTACATG486100.019.1366271
TACATGG488950.018.4753722
GCAGAGT658850.018.2424039
AGAGTAC583150.018.20881310-11
ACATGGG488100.018.0851823
GAGTACT375050.017.62466212-13
CAGAGTA643650.016.6669110-11
CATGGGG343100.015.9486044
AGTACTT435200.015.36241212-13
GTACTTT432700.015.29181614-15
TATACTG116650.013.1763565
Produced by FastQC (version 0.11.5)