FastQCFastQC Report
Fri 27 May 2016
SRR2139816_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139816_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15292167
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160900.048.0621071
GTATCAA291100.035.329261
TCAACGC356700.028.0659354
ATCAACG364250.027.4957163
CAACGCA370050.027.132795
AACGCAG380150.026.4339896
TATCAAC389200.025.9391842
ACGCAGA442550.022.7352127
CGCAGAG453000.022.2200098
GTACATG401950.019.13481
TACATGG405950.018.4087732
GCAGAGT542900.018.3937079
AGAGTAC484400.018.207110-11
ACATGGG407950.017.8856533
GAGTACT302200.017.85509912-13
CAGAGTA532850.016.48466910-11
CATGGGG303600.015.8516264
AGTACTT346000.015.83129812-13
GTACTTT349950.015.35343814-15
TGGTATC78850.015.2194112
Produced by FastQC (version 0.11.5)