##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139816_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15292167
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.087316205741146	33.0	31.0	34.0	30.0	34.0
2	32.239235943473545	33.0	31.0	34.0	30.0	34.0
3	32.289433014954646	34.0	31.0	34.0	30.0	34.0
4	35.93327498973821	37.0	35.0	37.0	35.0	37.0
5	35.815549947891625	37.0	35.0	37.0	35.0	37.0
6	35.82919373035882	37.0	35.0	37.0	35.0	37.0
7	35.74927039444442	37.0	35.0	37.0	33.0	37.0
8	35.7298907342563	37.0	35.0	37.0	33.0	37.0
9	37.36835747346991	39.0	37.0	39.0	34.0	39.0
10-11	37.281938851439435	39.0	37.0	39.0	34.0	39.0
12-13	37.263267037300864	39.0	37.0	39.0	33.5	39.0
14-15	38.48839069047572	40.0	38.0	41.0	34.0	41.0
16-17	38.471672654372654	40.0	38.0	41.0	34.0	41.0
18-19	38.50752679460014	40.0	38.0	41.0	34.0	41.0
20-21	38.47981799174004	40.0	38.0	41.0	34.0	41.0
22-23	38.41564429160584	40.0	38.0	41.0	34.0	41.0
24-25	38.333229472268634	40.0	38.0	41.0	34.0	41.0
26-27	38.19349277040162	40.0	38.0	41.0	33.0	41.0
28-29	38.097524319677206	40.0	37.5	41.0	33.0	41.0
30-31	37.98776434515845	40.0	37.0	41.0	33.0	41.0
32-33	38.1507651705681	40.0	37.5	41.0	33.0	41.0
34-35	38.14042169378408	40.0	38.0	41.0	33.0	41.0
36-37	38.059118994192744	40.0	37.0	41.0	33.0	41.0
38-39	37.93411157736796	40.0	37.0	41.0	33.0	41.0
40-41	37.8853633080068	40.0	37.0	41.0	33.0	41.0
42-43	37.80861909913034	40.0	37.0	41.0	33.0	41.0
44-45	37.80652509113328	40.0	37.0	41.0	33.0	41.0
46-47	37.84308327481445	40.0	37.0	41.0	33.0	41.0
48-49	37.77306827110621	40.0	37.0	41.0	33.0	41.0
50-51	37.707580561867104	40.0	37.0	41.0	32.5	41.0
52-53	37.571848780214545	40.0	36.5	41.0	32.0	41.0
54-55	37.44808078458446	39.0	36.0	41.0	32.0	41.0
56-57	37.281183174575446	39.0	36.0	41.0	31.0	41.0
58-59	37.11732265165283	39.0	35.5	40.5	31.0	41.0
60-61	36.96139481833787	39.0	35.0	40.0	31.0	41.0
62-63	36.737704660598496	38.0	35.0	40.0	31.0	41.0
64-65	36.52088780771311	38.0	35.0	40.0	31.0	41.0
66-67	36.265352241246916	37.5	35.0	40.0	30.5	41.0
68-69	35.97353549192123	37.0	34.0	40.0	30.0	41.0
70-71	35.68724386059205	36.5	34.0	39.0	30.0	41.0
72-73	35.36812155011009	36.0	34.0	39.0	30.0	40.0
74-75	35.02662817805917	35.5	34.0	38.5	29.5	40.0
76-77	33.941578488364605	35.0	32.5	37.0	28.5	39.0
78-79	34.289206818693884	35.0	33.0	37.0	29.0	39.0
80-81	34.17987669225916	35.0	33.0	37.0	29.0	39.0
82-83	33.92961840932904	35.0	33.0	36.0	29.0	38.5
84-85	33.713892541611	35.0	33.0	36.0	29.0	37.0
86-87	33.53089640491906	35.0	33.0	35.5	29.0	37.0
88-89	33.32441447785742	35.0	33.0	35.0	29.0	36.5
90-91	33.20620923409811	35.0	33.0	35.0	29.0	36.0
92-93	33.14091280262112	35.0	33.0	35.0	29.0	36.0
94-95	33.10547073457855	34.5	33.0	35.0	29.0	36.0
96-97	33.057829069241315	34.0	33.0	35.0	29.0	35.0
98-99	33.13670022178128	34.0	33.0	35.0	29.0	35.0
100-101	32.626065542649826	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	16.0
20	81.0
21	511.0
22	2424.0
23	8099.0
24	21761.0
25	46870.0
26	84181.0
27	134110.0
28	191789.0
29	258815.0
30	332476.0
31	417156.0
32	524190.0
33	672538.0
34	892224.0
35	1228622.0
36	1858032.0
37	3069688.0
38	3663615.0
39	1805891.0
40	79073.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.117161550746864	22.28497766209328	15.487164114804658	24.1106966723552
2	13.969895956379094	22.987447918189154	40.9210383736343	22.121617751797444
3	16.84070674875575	27.997366233314086	31.706794726999775	23.455132290930383
4	12.257746073529017	19.75258313618992	39.571527043878085	28.41814374640298
5	12.689097627563184	38.4027195099295	36.26301622261907	12.64516663988825
6	28.416973212495	39.59285822604475	19.326776904803616	12.66339165665664
7	24.86268950633354	33.24986576461008	24.84598160613862	17.041463122917765
8	23.55827006074417	37.40091250638317	22.002460475353168	17.038356957519493
9	25.214052396890512	16.79584064181355	21.887466962661343	36.102639998634594
10-11	23.499073087548677	27.218496894521227	29.9455825979405	19.336847419989596
12-13	24.48586913810188	24.900506252645556	29.755674915137924	20.85794969411464
14-15	21.739613489703586	26.169479446568957	27.214903551602593	24.876003512124868
16-17	21.342939820105286	29.11904506405142	27.896719935114493	21.6412951807288
18-19	21.41459244296061	28.451598151763	29.35774984008886	20.776059565187524
20-21	22.178570428947697	27.674520850450723	29.121667716925543	21.025241003676037
22-23	21.935648054025112	27.577956737568172	28.990926846978194	21.495468361428518
24-25	21.772621113087943	27.75582655556208	28.93952444912023	21.532027882229748
26-27	21.717100637041217	27.869556042339923	28.901912223121396	21.511431097497464
28-29	21.691827806740523	27.889520017412238	28.93527562235281	21.48337655349443
30-31	21.905216212017628	27.764967957058463	28.837752661396205	21.492063169527707
32-33	21.73951629207264	27.9236009365735	28.763942399960886	21.57294037139297
34-35	21.85856008427365	27.95421972233373	28.741657562868955	21.445562630523668
36-37	21.838133255036258	27.903472139295616	28.70723352769396	21.551161077974164
38-39	21.714114492481112	27.984912361011848	28.76994898274881	21.53102416375823
40-41	21.8996320661041	27.843710606010276	28.662850054664883	21.59380727322074
42-43	21.825312347745037	27.98942529709577	28.417873620437646	21.767388734721546
44-45	21.90526887689734	28.025995840328825	28.292991170682335	21.7757441120915
46-47	21.971972735649206	28.013659923998585	28.08887178301756	21.92549555733465
48-49	21.877285997577737	28.160111816789563	28.04691641232644	21.915685773306258
50-51	21.933129432791	28.212983121396167	27.929622844508277	21.924264601304557
52-53	22.061104422866244	28.082841337487412	27.856088453048166	21.99996578659818
54-55	22.014876459161105	27.996262188963676	27.909547561631996	22.079313790243223
56-57	22.01271516269646	28.072857609231082	27.879013678863867	22.03541354920859
58-59	22.008107936426903	28.082234937389707	27.862199995862998	22.047457130320396
60-61	22.065294642452102	28.05937991170822	27.877032396482203	21.99829304935747
62-63	22.03571308051771	28.138967656488596	27.857456743301174	21.967862519692524
64-65	22.00355989413053	28.110267768006942	27.79891991941505	22.08725241844748
66-67	22.04901575724179	28.180602980927606	27.658687564520562	22.111693697310038
68-69	22.006647645745296	28.151687125855783	27.69195952065356	22.149705707745362
70-71	22.009949079486624	28.01812771375149	27.763998097678037	22.207925109083845
72-73	22.04569434758813	28.003815141839965	27.668036876313074	22.282453634258825
74-75	21.976229741979846	28.07809317079703	27.662221094094047	22.283455993129078
76-77	22.15448196721766	27.989131032921993	27.622887718618305	22.233499281242043
78-79	22.120315047752513	27.982818356196766	27.58260349270089	22.31426310334983
80-81	22.118846086495115	27.98418714288941	27.555774903873438	22.34119186674204
82-83	22.17642253563405	27.952047080454577	27.628741836213074	22.242788547698304
84-85	22.177619453403096	27.950983160035943	27.628049050308213	22.243348336252748
86-87	22.085987802021137	27.98354810119623	27.68951969714107	22.24094439964156
88-89	22.144389255089685	27.947851867517244	27.673498335307308	22.234260542085764
90-91	22.180866227427952	27.93192250664306	27.70609175853681	22.18111950739218
92-93	22.24992450590236	27.817701160386317	27.715789311233546	22.216585022477776
94-95	22.107991200313187	27.81423374250342	27.803537263553064	22.27423779363033
96-97	22.045957587103764	27.867709861702107	27.85763106294453	22.228701488249595
98-99	22.22296264897132	28.424084207593335	27.991468069280995	21.361485074154356
100-101	22.76556169843584	31.10617763041299	25.182458925747802	20.945801745403365
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3104.0
1	2814.5
2	4036.0
3	5875.5
4	7782.5
5	11960.0
6	15356.5
7	17880.5
8	19508.0
9	19261.0
10	18161.0
11	17014.5
12	18654.0
13	24475.5
14	38119.5
15	57210.0
16	72715.0
17	81121.0
18	83296.5
19	81195.0
20	77067.0
21	73763.0
22	75067.5
23	84721.5
24	102246.5
25	127494.0
26	160235.0
27	195169.0
28	231010.5
29	267848.0
30	302093.5
31	336737.5
32	372780.0
33	406695.5
34	439954.5
35	472610.5
36	507164.5
37	536344.5
38	559601.5
39	581182.5
40	600475.0
41	617858.0
42	628417.0
43	636336.5
44	643641.0
45	647804.0
46	646922.5
47	641693.5
48	632785.5
49	619332.5
50	601892.5
51	578618.0
52	549073.5
53	516282.5
54	482278.0
55	445325.0
56	406341.0
57	366238.5
58	324003.0
59	279217.5
60	232320.5
61	187985.5
62	148973.0
63	114848.5
64	85894.0
65	64211.0
66	48712.5
67	37204.0
68	28263.0
69	20475.0
70	14566.5
71	10554.5
72	7451.5
73	5178.0
74	3512.0
75	2316.5
76	1493.5
77	962.0
78	660.5
79	454.0
80	331.5
81	244.0
82	181.5
83	124.5
84	83.0
85	65.5
86	51.0
87	38.5
88	25.0
89	12.5
90	6.5
91	4.5
92	3.5
93	3.0
94	2.5
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003923577345185937
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.264132938124466E-4
20-21	0.0012653955229129286
22-23	0.0026029784548268625
24-25	3.2707951842774186E-5
26-27	0.008104659456082791
28-29	2.2914232779602166E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.03487086746260816
38-39	0.015073084773037617
40-41	0.02479445410988918
42-43	0.0012540355797008244
44-45	0.005574068870443857
46-47	0.004556514164946643
48-49	0.0025284458905585104
50-51	0.002246177763002606
52-53	1.496834088985076E-4
54-55	0.0026713345631539263
56-57	0.01465813334371043
58-59	0.010033422739344438
60-61	0.003383692034955725
62-63	0.0010973722765411096
64-65	0.003115046895270988
66-67	2.718461604965182E-4
68-69	5.542451769817249E-4
70-71	1.96244762276356E-4
72-73	0.0
74-75	4.023919303034643E-6
76-77	0.006431844297521955
78-79	3.924469738042058E-4
80-81	8.380602375071093E-6
82-83	1.1904965888658722E-4
84-85	0.0018329569014902975
86-87	0.005479930760286994
88-89	0.08248673997862815
90-91	0.002998563945665117
92-93	0.001349777088690213
94-95	4.699682973485657E-6
96-97	0.017744405572533706
98-99	3.019866493715557E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	298.0
20-21	1155.0
22-23	2523.0
24-25	5270.0
26-27	6574.0
28-29	12050.0
30-31	36174.0
32-33	47567.0
34-35	66092.0
36-37	102211.0
38-39	109917.0
40-41	118481.0
42-43	133618.0
44-45	157421.0
46-47	158803.0
48-49	143233.0
50-51	131617.0
52-53	119952.0
54-55	130970.0
56-57	138043.0
58-59	143160.0
60-61	143231.0
62-63	137382.0
64-65	148397.0
66-67	157680.0
68-69	160898.0
70-71	159918.0
72-73	154563.0
74-75	158370.0
76-77	160844.0
78-79	170412.0
80-81	173113.0
82-83	167624.0
84-85	167399.0
86-87	177601.0
88-89	185311.0
90-91	200522.0
92-93	206945.0
94-95	253980.0
96-97	325080.0
98-99	1234411.0
100-101	8883357.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.44680667454341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.08646137753041	76.97600449319563
2	6.973901837990567	11.917952842360458
3	1.6453854594931487	4.217787997872476
4	0.5914372361357086	2.021456927048567
5	0.268604511335589	1.147569887600114
6	0.1480551093441829	0.759050178318647
7	0.08031221865984474	0.48036958350019887
8	0.05129322842528413	0.35062740583747587
9	0.031645117601601276	0.24335768213074935
>10	0.1191617194422959	1.6096045676146156
>50	0.003077391568325913	0.17362652184745786
>100	6.259962678065152E-4	0.08237287341377872
>500	3.879620528204658E-5	0.020219039259912554
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.307859115061979E-5
2	0.0	1.307859115061979E-5	0.0	1.307859115061979E-5	1.307859115061979E-5
3	0.0	1.9617886725929688E-5	0.0	1.307859115061979E-5	1.307859115061979E-5
4	0.0	5.231436460247916E-5	0.0	1.307859115061979E-5	1.307859115061979E-5
5	0.0	6.539295575309895E-5	0.0	1.307859115061979E-5	1.307859115061979E-5
6	0.0	6.539295575309895E-5	0.0	1.307859115061979E-5	3.9235773451859376E-5
7	0.0	6.539295575309895E-5	0.0	1.307859115061979E-5	3.9235773451859376E-5
8	0.0	6.539295575309895E-5	0.0	3.9235773451859376E-5	3.9235773451859376E-5
9	6.539295575309895E-6	1.6348238938274738E-4	0.0	5.885366017778906E-5	4.577506902716927E-5
10-11	1.9617886725929688E-5	1.6348238938274738E-4	0.0	8.17411946913737E-5	5.231436460247916E-5
12-13	1.9617886725929688E-5	1.6348238938274738E-4	0.0	8.828049026668359E-5	6.539295575309895E-5
14-15	1.9617886725929688E-5	1.9617886725929685E-4	0.0	1.1116802478026823E-4	7.847154690371875E-5
16-17	3.2696477876549475E-5	2.0271816283460675E-4	0.0	1.2751626371854298E-4	7.847154690371875E-5
18-19	4.250542123951432E-5	2.1579675398522655E-4	0.0	1.569430938074375E-4	9.155013805433854E-5
20-21	6.539295575309895E-5	2.2887534513584636E-4	0.0	2.059878106222617E-4	1.1116802478026823E-4
22-23	8.82804902666836E-5	2.648414708000508E-4	0.0	4.250542123951432E-4	1.1443767256792318E-4
24-25	9.808943362964844E-5	2.9753794867660023E-4	0.0	0.0010593658832002031	1.307859115061979E-4
26-27	1.0789837699261328E-4	3.13886187614875E-4	0.0	0.003050581385882066	1.307859115061979E-4
28-29	1.1770732035557811E-4	3.13886187614875E-4	0.0	0.018156354164847927	1.307859115061979E-4
30-31	1.2424661593088803E-4	3.4004336991611456E-4	0.0	0.027785466899491747	1.3405555929385285E-4
32-33	1.2424661593088803E-4	3.4985231327907943E-4	0.0	0.03541028554030309	1.6348238938274738E-4
34-35	1.2424661593088803E-4	4.2505421239514326E-4	0.0	0.045605047342211213	1.7656098053336718E-4
36-37	1.4059485486916275E-4	4.969864637235521E-4	0.0	0.05916754636540394	1.8636992389633203E-4
38-39	1.504037982321276E-4	4.969864637235521E-4	0.0	0.08290518930377885	2.0271816283460675E-4
40-41	1.569430938074375E-4	5.427615327507214E-4	0.0	0.12782361061058253	2.0271816283460675E-4
42-43	1.8636992389633203E-4	5.525704761136862E-4	0.0	0.14875916539493717	2.0271816283460675E-4
44-45	1.9617886725929688E-4	6.048848407161653E-4	0.0	0.17880068926791082	2.2560569734819138E-4
46-47	1.9944851504695183E-4	6.473902619556797E-4	0.0	0.21155275115685043	2.3868428849881118E-4
48-49	2.1579675398522655E-4	6.473902619556797E-4	0.0	0.23768377627578877	2.615718230123958E-4
50-51	2.2887534513584636E-4	7.062439221334687E-4	0.0	0.2613854530884995	2.615718230123958E-4
52-53	2.452235840741211E-4	7.062439221334687E-4	0.0	0.2944546708128416	2.779200619506706E-4
54-55	2.648414708000508E-4	7.160528654964336E-4	0.0	0.3618911564332249	2.877290053136354E-4
56-57	2.746504141630156E-4	7.258618088593984E-4	0.0	0.43262998631913974	2.877290053136354E-4
58-59	2.811897097383255E-4	7.324011044347083E-4	0.0	0.4896395651446914	2.909986531012904E-4
60-61	2.811897097383255E-4	7.454796955853281E-4	0.0	0.5246901894283524	3.073468920395651E-4
62-63	3.008075964642552E-4	7.749065256742226E-4	0.0	0.5451876114091613	3.073468920395651E-4
64-65	3.1061653982722003E-4	8.697263115162161E-4	0.0	0.5590378394376676	3.073468920395651E-4
66-67	3.1715583540253E-4	8.76265607091526E-4	0.0	0.5667378599775951	3.1061653982722003E-4
68-69	3.204254831901849E-4	9.089620849680755E-4	0.0	0.5747419577617743	3.269647787654948E-4
70-71	3.204254831901849E-4	9.285799716940052E-4	0.0	0.5823602371070105	3.5312196106673436E-4
72-73	3.3350407434080465E-4	9.285799716940052E-4	0.0	0.5883796586840832	3.7927914336797396E-4
74-75	3.3350407434080465E-4	9.514675062075898E-4	0.0	0.5935293539496397	3.7927914336797396E-4
76-77	3.4658266549142446E-4	0.0010332087008989635	0.0	0.5984403649266974	3.7927914336797396E-4
78-79	3.4985231327907943E-4	0.001075714122138478	0.0	0.6030538379550785	3.7927914336797396E-4
80-81	3.5966125664204426E-4	0.0010789837699261328	0.0	0.605731679493168	3.7927914336797396E-4
82-83	3.6620055221735416E-4	0.0010789837699261328	0.0	0.6075561429586794	3.7927914336797396E-4
84-85	3.7927914336797396E-4	0.0010789837699261328	0.0	0.6090765291799389	3.7927914336797396E-4
86-87	3.825487911556289E-4	0.0010855230655014427	0.0	0.6093381010029514	3.7927914336797396E-4
88-89	3.8581843894328386E-4	0.0011116802478026823	0.0	0.6093838760719785	3.988970300939036E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16090	0.0	48.062107	1
GTATCAA	29110	0.0	35.32926	1
TCAACGC	35670	0.0	28.065935	4
ATCAACG	36425	0.0	27.495716	3
CAACGCA	37005	0.0	27.13279	5
AACGCAG	38015	0.0	26.433989	6
TATCAAC	38920	0.0	25.939184	2
ACGCAGA	44255	0.0	22.735212	7
CGCAGAG	45300	0.0	22.220009	8
GTACATG	40195	0.0	19.1348	1
TACATGG	40595	0.0	18.408773	2
GCAGAGT	54290	0.0	18.393707	9
AGAGTAC	48440	0.0	18.2071	10-11
ACATGGG	40795	0.0	17.885653	3
GAGTACT	30220	0.0	17.855099	12-13
CAGAGTA	53285	0.0	16.484669	10-11
CATGGGG	30360	0.0	15.851626	4
AGTACTT	34600	0.0	15.831298	12-13
GTACTTT	34995	0.0	15.353438	14-15
TGGTATC	7885	0.0	15.219411	2
>>END_MODULE