FastQCFastQC Report
Fri 27 May 2016
SRR2139815_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139815_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11718222
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127750.046.526281
GTATCAA225450.034.2412071
TCAACGC269000.028.0065024
AGACCGA96750.027.74827490-91
ATCAACG271900.027.7077943
CGAGACC96400.027.63573588-89
ACCGAGG100650.027.62778592-93
GAGACCG98800.027.54006888-89
GACCGAG99950.027.38173390-91
CAACGCA277050.027.1775175
ACGAGAC101150.026.8412386-87
AACGCAG285100.026.4841186
TATCAAC292000.025.9027392
CCCACGA104650.025.27828884-85
CACGAGA106900.024.98562886-87
GCCCACG105500.024.95321382-83
CCACGAG112500.024.00110684-85
ATCTCCG108550.023.42321274-75
ACGCAGA325200.023.2443377
TCTCCGA108400.023.11638576-77
Produced by FastQC (version 0.11.5)