##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139815_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11718222
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.042167659906085	33.0	31.0	34.0	30.0	34.0
2	32.19780970184726	33.0	31.0	34.0	30.0	34.0
3	32.249187547394136	34.0	31.0	34.0	30.0	34.0
4	35.90093454450684	37.0	35.0	37.0	35.0	37.0
5	35.77832003865433	37.0	35.0	37.0	35.0	37.0
6	35.792519633097925	37.0	35.0	37.0	33.0	37.0
7	35.713326219626154	37.0	35.0	37.0	33.0	37.0
8	35.691918961767406	37.0	35.0	37.0	33.0	37.0
9	37.32694413879512	39.0	37.0	39.0	34.0	39.0
10-11	37.23736271594787	39.0	37.0	39.0	33.0	39.0
12-13	37.21772176700527	39.0	37.0	39.0	33.0	39.0
14-15	38.43322263394566	40.0	38.0	41.0	33.0	41.0
16-17	38.41650094186644	40.0	38.0	41.0	33.0	41.0
18-19	38.451684607101654	40.0	38.0	41.0	33.5	41.0
20-21	38.426056905943184	40.0	38.0	41.0	34.0	41.0
22-23	38.35942332849581	40.0	38.0	41.0	34.0	41.0
24-25	38.27978069706862	40.0	38.0	41.0	33.5	41.0
26-27	38.13856373487113	40.0	38.0	41.0	33.0	41.0
28-29	38.04014718499545	40.0	37.0	41.0	33.0	41.0
30-31	37.93126716603061	40.0	37.0	41.0	33.0	41.0
32-33	38.09632139775499	40.0	37.0	41.0	33.0	41.0
34-35	38.08291355999512	40.0	37.0	41.0	33.0	41.0
36-37	38.001938444277194	40.0	37.0	41.0	33.0	41.0
38-39	37.87848214839845	40.0	37.0	41.0	33.0	41.0
40-41	37.830476074389935	40.0	37.0	41.0	33.0	41.0
42-43	37.75548513541945	40.0	37.0	41.0	33.0	41.0
44-45	37.75615603997138	40.0	37.0	41.0	33.0	41.0
46-47	37.7948342768357	40.0	37.0	41.0	33.0	41.0
48-49	37.72642115233918	40.0	37.0	41.0	32.5	41.0
50-51	37.667425661784364	40.0	37.0	41.0	32.0	41.0
52-53	37.53513305820239	40.0	36.0	41.0	32.0	41.0
54-55	37.420340243646066	39.0	36.0	41.0	32.0	41.0
56-57	37.25982633194596	39.0	36.0	41.0	31.0	41.0
58-59	37.103768974247174	39.0	35.5	40.5	31.0	41.0
60-61	36.95723124258148	39.0	35.0	40.0	31.0	41.0
62-63	36.742912437287096	38.5	35.0	40.0	31.0	41.0
64-65	36.53688471424783	38.0	35.0	40.0	31.0	41.0
66-67	36.293553507481676	38.0	35.0	40.0	30.5	41.0
68-69	36.00764717769847	37.0	34.0	40.0	30.0	41.0
70-71	35.72963360172545	37.0	34.0	39.0	30.0	41.0
72-73	35.41152328086562	36.0	34.0	39.0	30.0	40.5
74-75	35.072107677580945	36.0	34.0	39.0	29.0	40.0
76-77	33.977039267239704	35.0	32.5	37.0	28.5	39.0
78-79	34.329148137597656	35.0	33.0	37.0	29.0	39.0
80-81	34.2056012119771	35.0	33.0	37.0	29.0	39.0
82-83	33.94110993732431	35.0	33.0	36.0	29.0	38.5
84-85	33.719404974332114	35.0	33.0	36.0	29.0	37.0
86-87	33.53154892125316	35.0	33.0	35.5	29.0	37.0
88-89	33.318270295471756	35.0	33.0	35.0	29.0	36.5
90-91	33.1879225088712	35.0	33.0	35.0	29.0	36.0
92-93	33.12486902971828	35.0	33.0	35.0	29.0	36.0
94-95	33.080670883813866	34.0	33.0	35.0	29.0	36.0
96-97	33.02963620579893	34.0	33.0	35.0	29.0	35.0
98-99	33.11343021311791	34.0	33.0	35.0	29.0	35.0
100-101	32.61042821259586	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	4.0
19	11.0
20	68.0
21	409.0
22	1893.0
23	6526.0
24	17451.0
25	37214.0
26	67008.0
27	106178.0
28	153336.0
29	205046.0
30	262452.0
31	329638.0
32	411812.0
33	523594.0
34	692438.0
35	943581.0
36	1416927.0
37	2325073.0
38	2825343.0
39	1339580.0
40	52638.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.74898615165338	22.537625588591855	15.578813919039936	24.134574340714828
2	13.788909107015183	23.13703696560383	41.0755624070748	21.99849152030619
3	16.746038776189767	28.051875105284747	31.832943598440107	23.369142520085383
4	12.062350414593613	19.99520917081107	39.89220378313365	28.050236631461665
5	12.533761521159098	38.441702162665976	36.52532781850352	12.499208497671404
6	28.11254130532772	39.954559659306675	19.478193876169954	12.454705159195653
7	24.60501260344786	33.293771017480296	25.04389317765101	17.05732320142083
8	23.269059077392455	37.699021233767375	22.183476298708115	16.848443390132054
9	24.993237028620896	16.804878760617438	22.045358075653457	36.15652613510821
10-11	23.254449352470026	27.41511468207378	30.161704565760918	19.168731399695275
12-13	24.23522527564335	25.047268263052192	29.956635059482572	20.76087140182188
14-15	21.46325611513419	26.396423450588323	27.406555363091773	24.733765071185715
16-17	21.104925303514474	29.32725203533437	28.10788616225226	21.459936498898895
18-19	21.154422347203976	28.656384046806004	29.591480058080815	20.597713547909205
20-21	21.9185497570726	27.907077864225645	29.35291203728615	20.82146034141561
22-23	21.640962178828328	27.86945677632811	29.163826592178623	21.325754452664942
24-25	21.490452114077783	28.00466061607823	29.19322271946615	21.31166455037783
26-27	21.487378847246482	28.093782207848193	29.09810231652029	21.320736628385035
28-29	21.44781733941345	28.161283051934717	29.11242809819008	21.278471510461756
30-31	21.653866291082156	28.04912576267437	29.036780041421846	21.260227904821623
32-33	21.509284479752104	28.15775094954054	28.979186311439864	21.353778259267493
34-35	21.595861737709576	28.189064677852826	28.988015712968913	21.227057871468684
36-37	21.57030738397707	28.167633366818666	28.922486424346605	21.339572824857655
38-39	21.458835898204683	28.250313805005085	28.963676513112365	21.327173783677875
40-41	21.659921006859033	28.11065351122386	28.86090207686261	21.3685234050545
42-43	21.573018988709844	28.25231222918212	28.64950957853797	21.52515920357007
44-45	21.64630860163278	28.279591423535273	28.504525835495826	21.569574139336126
46-47	21.719927482072574	28.304562320465553	28.29442581279249	21.681084384669383
48-49	21.65879872225299	28.390882537460556	28.2716001726508	21.67871856763566
50-51	21.680871817950518	28.465123016941025	28.170950272594773	21.68305489251368
52-53	21.8028672479197	28.356187348029334	28.091314250490683	21.749631153560287
54-55	21.75770849718556	28.307787193013795	28.140664935327276	21.79383937447337
56-57	21.773635200899534	28.35992333017613	28.101987576203086	21.764453892721253
58-59	21.776809445372784	28.365495688987313	28.119564155067735	21.73813071057217
60-61	21.73586576759438	28.19659542648672	28.230995472986493	21.836543332932408
62-63	21.865501016237264	28.315730587251643	28.202213408129563	21.616554988381534
64-65	21.624248794943675	28.269117023744144	28.162850006310325	21.943784175001856
66-67	21.709560398797027	28.27861018773148	28.039175334185263	21.97265407928623
68-69	21.65766568517413	28.50495523709616	28.0648493547497	21.772529722980007
70-71	21.686408017538668	28.343283639623014	28.10585689493637	21.864451447901946
72-73	21.688439318318565	28.334102704031228	27.834137540068504	22.1433204375817
74-75	21.62863871370251	28.39068599466641	27.827704777077262	22.152970514553814
76-77	21.764382054703063	28.09153414319592	28.02800560326774	22.11607819883328
78-79	21.74711063465253	28.052842334343524	27.96267736384772	22.237369667156223
80-81	22.011128956755314	28.048508796669996	27.75989699370904	22.18046525286564
82-83	22.016093137328284	28.28476828491305	27.840109837052335	21.85902874070633
84-85	21.803980384781944	28.035762385125818	27.80961174155013	22.35064548854211
86-87	21.739452964512143	28.285777322143968	27.845154129268536	22.129615584075353
88-89	22.04866290747824	28.002361092606154	27.83238810895424	22.116587890961366
90-91	22.079350529550155	28.187610556574526	27.894991170037724	21.838047743837596
92-93	21.830333377112726	28.130443134068283	27.908769914136627	22.130453574682367
94-95	21.966728784230206	27.861536320623436	28.021103837171314	22.150631057975048
96-97	21.90507591792576	28.1894585869514	28.06652464882221	21.83894084630063
98-99	22.17113749639725	28.588327745431936	28.223374597253283	21.017160160917538
100-101	22.51771998032133	31.35442088817436	25.51622316406348	20.61163596744083
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2268.0
1	2036.5
2	2835.0
3	4105.0
4	5549.5
5	8732.0
6	11318.0
7	13178.0
8	14203.5
9	13871.5
10	13034.0
11	12205.0
12	13449.0
13	17928.0
14	28412.0
15	42889.0
16	54590.5
17	60792.0
18	62294.5
19	60893.5
20	58404.5
21	56623.5
22	57905.0
23	64762.5
24	78456.5
25	99436.5
26	125668.5
27	154001.0
28	184191.5
29	214526.0
30	242161.0
31	270807.0
32	300793.5
33	329287.0
34	355874.0
35	379829.0
36	405333.5
37	428125.0
38	445417.5
39	459384.5
40	473451.0
41	486285.0
42	490870.0
43	493865.5
44	497277.5
45	497488.5
46	493762.5
47	486743.5
48	477489.0
49	465163.5
50	450950.0
51	431349.0
52	404900.5
53	376554.0
54	347778.0
55	318090.0
56	287886.0
57	256929.5
58	225267.0
59	192243.5
60	159346.5
61	128987.5
62	101661.0
63	78048.0
64	57972.5
65	43403.5
66	32889.0
67	24688.5
68	18602.5
69	13373.5
70	9510.5
71	6909.5
72	4865.0
73	3326.5
74	2253.0
75	1527.0
76	1051.5
77	718.0
78	486.0
79	328.0
80	236.0
81	157.0
82	108.5
83	86.0
84	69.5
85	52.0
86	36.0
87	25.0
88	16.0
89	10.5
90	8.0
91	6.5
92	5.0
93	3.0
94	2.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00395964507243505
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.717590945110956E-4
20-21	0.0013398473811424162
22-23	0.00265436429980729
24-25	4.695273545569657E-5
26-27	0.008151450795981272
28-29	1.9223903668249762E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.03502348626158919
38-39	0.015341399881419594
40-41	0.024726631164546767
42-43	0.001192505342423934
44-45	0.0053266409921085035
46-47	0.004558968044246857
48-49	0.002567299900594512
50-51	0.0022335800841866665
52-53	2.1789523495110036E-4
54-55	0.002730665996344274
56-57	0.014423666215596714
58-59	0.009986916900509095
60-61	0.0037165823498017574
62-63	0.0011435415510910381
64-65	0.0032162521283193544
66-67	2.8218232483185647E-4
68-69	4.4592600995226685E-4
70-71	1.978087356902498E-4
72-73	0.0
74-75	0.0
76-77	0.0057831202559601655
78-79	3.0920310523082625E-4
80-81	0.0
82-83	1.0967023312436951E-4
84-85	0.0018905769640536817
86-87	0.005223482407723037
88-89	0.08030685593933043
90-91	0.0028835265004823352
92-93	0.0013643798158158432
94-95	6.050411666984616E-6
96-97	0.01687676198884298
98-99	3.89574088388517E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	274.0
20-21	990.0
22-23	1967.0
24-25	4154.0
26-27	5144.0
28-29	8867.0
30-31	26176.0
32-33	34172.0
34-35	48875.0
36-37	74969.0
38-39	81084.0
40-41	87690.0
42-43	98924.0
44-45	115565.0
46-47	117598.0
48-49	107507.0
50-51	97184.0
52-53	89925.0
54-55	97735.0
56-57	104001.0
58-59	61552.0
60-61	109155.0
62-63	104126.0
64-65	110993.0
66-67	120121.0
68-69	121211.0
70-71	120474.0
72-73	117066.0
74-75	119512.0
76-77	120327.0
78-79	129044.0
80-81	131656.0
82-83	127041.0
84-85	126465.0
86-87	133563.0
88-89	142339.0
90-91	153514.0
92-93	158924.0
94-95	195161.0
96-97	250931.0
98-99	992879.0
100-101	6869367.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.36460568858439
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.4236260857976	78.09400811831902
2	6.844494517130434	11.822441402192954
3	1.6211919983971583	4.200408230611761
4	0.5272558933622137	1.8214498930883953
5	0.2269965481020681	0.9802233684752445
6	0.11557060846565385	0.5988726017595581
7	0.06888141422526498	0.416424132518594
8	0.042562281721217655	0.29406997424475245
9	0.02849645444319121	0.22149765463580257
>10	0.09818587917921362	1.3511687629559177
>50	0.0023033854464314008	0.13406987409218496
>100	4.349337295445621E-4	0.06536598710579151
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.533717828523815E-6	0.0	0.0	0.0
3	0.0	8.533717828523815E-6	0.0	0.0	0.0
4	0.0	3.413487131409526E-5	0.0	1.706743565704763E-5	0.0
5	0.0	3.413487131409526E-5	0.0	3.413487131409526E-5	0.0
6	3.413487131409526E-5	3.413487131409526E-5	0.0	3.413487131409526E-5	8.533717828523815E-6
7	3.413487131409526E-5	3.413487131409526E-5	0.0	4.2668589142619074E-5	8.533717828523815E-6
8	3.413487131409526E-5	3.413487131409526E-5	0.0	4.2668589142619074E-5	8.533717828523815E-6
9	3.413487131409526E-5	5.9736024799666707E-5	0.0	8.533717828523815E-5	8.533717828523815E-6
10-11	3.413487131409526E-5	5.9736024799666707E-5	0.0	8.533717828523815E-5	8.533717828523815E-6
12-13	3.413487131409526E-5	6.826974262819052E-5	0.0	8.960403719950006E-5	8.533717828523815E-6
14-15	3.413487131409526E-5	7.680346045671433E-5	0.0	9.387089611376196E-5	1.706743565704763E-5
16-17	5.120230697114289E-5	7.680346045671433E-5	0.0	1.0240461394228578E-4	2.5601153485571445E-5
18-19	5.120230697114289E-5	8.960403719950006E-5	0.0	1.2373890851359533E-4	3.413487131409526E-5
20-21	5.120230697114289E-5	1.0240461394228578E-4	0.0	1.5360692091342866E-4	3.413487131409526E-5
22-23	5.120230697114289E-5	1.109383317708096E-4	0.0	2.9441326508407165E-4	4.2668589142619074E-5
24-25	5.120230697114289E-5	1.3227262634211913E-4	0.0	7.296328743387862E-4	5.120230697114289E-5
26-27	5.120230697114289E-5	1.4507320308490486E-4	0.0	0.0022913032369586443	5.120230697114289E-5
28-29	5.120230697114289E-5	1.4934006199916676E-4	0.0	0.013423538144267961	5.120230697114289E-5
30-31	5.54691658854048E-5	1.7920807439900013E-4	0.0	0.020340116444286516	5.120230697114289E-5
32-33	5.9736024799666707E-5	1.8774179222752393E-4	0.0	0.025148866440659685	5.120230697114289E-5
34-35	6.400288371392861E-5	2.218766635416192E-4	0.0	0.03175823089885138	5.9736024799666707E-5
36-37	6.826974262819052E-5	2.5601153485571446E-4	0.0	0.04096184557691431	5.9736024799666707E-5
38-39	6.826974262819052E-5	2.6454525268423826E-4	8.533717828523815E-6	0.05833222821687454	6.826974262819052E-5
40-41	6.826974262819052E-5	2.816126883412859E-4	8.533717828523815E-6	0.0957824489073513	7.680346045671433E-5
42-43	6.826974262819052E-5	2.816126883412859E-4	8.533717828523815E-6	0.11133941650875022	8.107031937097623E-5
44-45	6.826974262819052E-5	2.858795472555478E-4	8.533717828523815E-6	0.13410737567525177	8.533717828523815E-5
46-47	7.253660154245243E-5	2.901464061698097E-4	8.533717828523815E-6	0.15988347037630796	8.960403719950006E-5
48-49	7.680346045671433E-5	2.901464061698097E-4	8.533717828523815E-6	0.18172552115841464	1.109383317708096E-4
50-51	9.387089611376196E-5	3.7548358445504786E-4	8.533717828523815E-6	0.20322195636846613	1.109383317708096E-4
52-53	1.0240461394228578E-4	3.840173022835717E-4	8.533717828523815E-6	0.2323475353172179	1.109383317708096E-4
54-55	1.0240461394228578E-4	3.8828416119783364E-4	8.533717828523815E-6	0.29221583274322677	1.109383317708096E-4
56-57	1.0240461394228578E-4	4.010847379406193E-4	8.533717828523815E-6	0.3561419129966986	1.109383317708096E-4
58-59	1.0240461394228578E-4	4.010847379406193E-4	8.533717828523815E-6	0.41114599126044893	1.1947204959933341E-4
60-61	1.109383317708096E-4	4.010847379406193E-4	8.533717828523815E-6	0.8276426235993822	1.2800576742785723E-4
62-63	1.3653948525638103E-4	4.010847379406193E-4	8.533717828523815E-6	0.8372771910277856	1.3653948525638103E-4
64-65	1.3653948525638103E-4	4.266858914261908E-4	8.533717828523815E-6	0.8470568316592739	1.3653948525638103E-4
66-67	1.5360692091342866E-4	4.266858914261908E-4	8.533717828523815E-6	0.855270535069228	1.3653948525638103E-4
68-69	1.621406387419525E-4	4.437533270832384E-4	8.533717828523815E-6	0.8632239600854122	1.3653948525638103E-4
70-71	1.664074976562144E-4	4.6082076274028605E-4	8.533717828523815E-6	0.8704392185094292	1.3653948525638103E-4
72-73	1.9200865114178586E-4	4.6082076274028605E-4	8.533717828523815E-6	0.8765408267568237	1.4507320308490486E-4
74-75	1.9627551005604776E-4	4.906887751401194E-4	8.533717828523815E-6	0.882019473602736	1.4507320308490486E-4
76-77	1.9627551005604776E-4	5.333573642827385E-4	8.533717828523815E-6	0.8871439711587645	1.4507320308490486E-4
78-79	1.9627551005604776E-4	5.461579410255241E-4	8.533717828523815E-6	0.8913766952017124	1.4507320308490486E-4
80-81	1.9627551005604776E-4	5.461579410255241E-4	8.533717828523815E-6	0.8938685408076413	1.578737798276906E-4
82-83	2.0054236897030966E-4	5.461579410255241E-4	8.533717828523815E-6	0.8953107391206618	1.7920807439900013E-4
84-85	2.133429457130954E-4	5.54691658854048E-4	8.533717828523815E-6	0.8965822630771119	1.7920807439900013E-4
86-87	2.218766635416192E-4	5.54691658854048E-4	8.533717828523815E-6	0.8969065443545958	1.7920807439900013E-4
88-89	2.4747781702719066E-4	5.717590945110956E-4	8.533717828523815E-6	0.8969449460848241	1.8347493331326203E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12775	0.0	46.52628	1
GTATCAA	22545	0.0	34.241207	1
TCAACGC	26900	0.0	28.006502	4
AGACCGA	9675	0.0	27.748274	90-91
ATCAACG	27190	0.0	27.707794	3
CGAGACC	9640	0.0	27.635735	88-89
ACCGAGG	10065	0.0	27.627785	92-93
GAGACCG	9880	0.0	27.540068	88-89
GACCGAG	9995	0.0	27.381733	90-91
CAACGCA	27705	0.0	27.177517	5
ACGAGAC	10115	0.0	26.84123	86-87
AACGCAG	28510	0.0	26.484118	6
TATCAAC	29200	0.0	25.902739	2
CCCACGA	10465	0.0	25.278288	84-85
CACGAGA	10690	0.0	24.985628	86-87
GCCCACG	10550	0.0	24.953213	82-83
CCACGAG	11250	0.0	24.001106	84-85
ATCTCCG	10855	0.0	23.423212	74-75
ACGCAGA	32520	0.0	23.244337	7
TCTCCGA	10840	0.0	23.116385	76-77
>>END_MODULE