FastQCFastQC Report
Fri 27 May 2016
SRR2139814_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139814_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7654067
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79900.046.91651
TATCTCG95450.036.77597894-95
GTATCAA139400.034.841861
CCGTCTT40700.034.4812292-93
TATGCCG40000.032.8813188-89
TGCCGTC42300.031.90702690-91
ATGCCGT40850.030.40040688-89
GCCGTCT42900.029.5689290-91
CTCGTAT45750.029.22722284-85
CGTATGC45700.028.98044886-87
TCAACGC165600.028.7858454
ATCAACG167450.028.4678173
CAACGCA170500.027.933475
TCGTATG45500.027.21323884-85
AACGCAG175400.027.201916
TATCAAC179850.026.696282
GCTATCT150000.026.43519894-95
TCTCGTA47750.025.97478382-83
CTATCTC153100.025.58152494-95
ACGCAGA204600.023.3406377
Produced by FastQC (version 0.11.5)