##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139814_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7654067
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.095508701452445	33.0	31.0	34.0	30.0	34.0
2	32.25009527614535	34.0	31.0	34.0	30.0	34.0
3	32.30311192729303	34.0	31.0	34.0	30.0	34.0
4	35.93994944125783	37.0	35.0	37.0	35.0	37.0
5	35.81956834190242	37.0	35.0	37.0	35.0	37.0
6	35.83123978402593	37.0	35.0	37.0	35.0	37.0
7	35.75273838078501	37.0	35.0	37.0	33.0	37.0
8	35.73285378871128	37.0	35.0	37.0	33.0	37.0
9	37.38000242224167	39.0	37.0	39.0	34.0	39.0
10-11	37.29113613716734	39.0	37.0	39.0	34.0	39.0
12-13	37.270717058003285	39.0	37.0	39.0	33.5	39.0
14-15	38.50410748952159	40.0	38.0	41.0	34.0	41.0
16-17	38.48318325407917	40.0	38.0	41.0	34.0	41.0
18-19	38.51441070479263	40.0	38.0	41.0	34.0	41.0
20-21	38.48520747223559	40.0	38.0	41.0	34.0	41.0
22-23	38.41827621431584	40.0	38.0	41.0	34.0	41.0
24-25	38.33615957874699	40.0	38.0	41.0	34.0	41.0
26-27	38.19368520240631	40.0	38.0	41.0	33.0	41.0
28-29	38.0959785849262	40.0	37.5	41.0	33.0	41.0
30-31	37.98863150561572	40.0	37.0	41.0	33.0	41.0
32-33	38.160979359443175	40.0	38.0	41.0	33.0	41.0
34-35	38.15062166268473	40.0	38.0	41.0	33.0	41.0
36-37	38.0699098765189	40.0	37.5	41.0	33.0	41.0
38-39	37.94803410834204	40.0	37.0	41.0	33.0	41.0
40-41	37.89867004465627	40.0	37.0	41.0	33.0	41.0
42-43	37.81926991952241	40.0	37.0	41.0	33.0	41.0
44-45	37.81690777809764	40.0	37.0	41.0	33.0	41.0
46-47	37.8513561386781	40.0	37.0	41.0	33.0	41.0
48-49	37.77667320759434	40.0	37.0	41.0	33.0	41.0
50-51	37.710693878583285	40.0	37.0	41.0	32.5	41.0
52-53	37.57275279715169	40.0	36.5	41.0	32.0	41.0
54-55	37.45695147724921	39.0	36.0	41.0	32.0	41.0
56-57	37.29251487709752	39.0	36.0	41.0	31.0	41.0
58-59	37.13378955914312	39.0	35.5	41.0	31.0	41.0
60-61	36.98423189855903	39.0	35.0	40.0	31.0	41.0
62-63	36.762359539933485	38.5	35.0	40.0	31.0	41.0
64-65	36.55936503695124	38.0	35.0	40.0	31.0	41.0
66-67	36.30993612427612	37.5	35.0	40.0	31.0	41.0
68-69	36.01908581977582	37.0	34.0	40.0	30.0	41.0
70-71	35.72696373621727	36.5	34.0	39.0	30.0	41.0
72-73	35.40741698127907	36.0	34.0	39.0	30.0	40.5
74-75	35.05439361652532	36.0	34.0	38.5	29.5	40.0
76-77	33.94196501176003	35.0	32.5	37.0	28.5	39.0
78-79	34.25654077089166	35.0	33.0	37.0	29.0	39.0
80-81	34.117037219303924	35.0	33.0	36.5	29.0	39.0
82-83	33.856209642491905	35.0	33.0	36.0	29.0	37.5
84-85	33.65254513519594	35.0	33.0	36.0	29.0	37.0
86-87	33.48116960870334	35.0	33.0	35.0	29.0	37.0
88-89	33.273262026789496	35.0	33.0	35.0	29.0	36.0
90-91	33.172085906589146	35.0	33.0	35.0	29.0	36.0
92-93	33.12250421486783	35.0	33.0	35.0	29.0	36.0
94-95	33.084294466268815	35.0	33.0	35.0	29.0	35.5
96-97	33.022931351772115	34.0	33.0	35.0	29.0	35.0
98-99	33.09852050230859	34.0	33.0	35.0	29.0	35.0
100-101	32.584143491696295	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	2.0
19	7.0
20	45.0
21	263.0
22	1203.0
23	4184.0
24	10951.0
25	23783.0
26	43158.0
27	69512.0
28	99207.0
29	133657.0
30	170226.0
31	212443.0
32	265958.0
33	337464.0
34	446799.0
35	613354.0
36	931938.0
37	1550896.0
38	1882658.0
39	820368.0
40	35990.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.274193089006545	22.04924807285435	15.300795681667756	24.375763156471344
2	13.974881511943533	22.799943818206167	40.8160862847829	22.409088385067403
3	17.008306434775974	27.68339076250453	31.485631297143247	23.822671505576253
4	12.320548017152188	19.561025530610067	39.39546126262025	28.722965189617494
5	12.80285369856313	38.24076794728868	36.23542882496325	12.72094952918494
6	28.743437965724628	39.34710788395242	19.209212566338916	12.700241583984043
7	25.075610129882588	33.013181097055984	24.631963634496536	17.279245138564896
8	23.604248564847943	37.37052471581448	21.924644767285155	17.10058195205242
9	25.19895961179326	16.73258370126414	21.76275981942147	36.30569686752113
10-11	23.613292595073	27.126569067996016	29.834574198863717	19.42556413806727
12-13	24.539818147647395	24.73216034901186	29.653838257088566	21.074183246252172
14-15	21.776391673628652	26.081521502328208	27.045251044003866	25.096835780039278
16-17	21.505037779261666	29.022080679461	27.742884926405793	21.729996614871546
18-19	21.52362600378572	28.36268869812794	29.158472719709266	20.955212578377072
20-21	22.245203867283667	27.63198286317356	28.928219940897808	21.194593328644963
22-23	22.05265229034399	27.543478742364798	28.756970577337693	21.646898389953524
24-25	21.867457557782778	27.6935704264055	28.770644408096157	21.668327607715568
26-27	21.82322659773244	27.802937288421482	28.704103233972333	21.66973287987374
28-29	21.806149898134343	27.823401298488704	28.744047590629236	21.626401212747716
30-31	21.993341273452153	27.71101904600592	28.64489198965711	21.650747690884813
32-33	21.842571158705717	27.88256877506693	28.569052808099755	21.705807258127603
34-35	21.94818345001524	27.879310454940587	28.574806202647736	21.597699892396438
36-37	21.924789128785026	27.832783343852658	28.54757443752399	21.694853089838322
38-39	21.78039801753392	27.896690417956254	28.63242698597041	21.690484578539415
40-41	21.952210597376567	27.683246936232226	28.57000387336201	21.794538593029195
42-43	21.975804697580976	27.82745057203838	28.34816233441593	21.848582395964712
44-45	21.903629810042847	27.81608439854591	28.25604405257986	22.02424173883139
46-47	21.987188534398943	27.827673626404824	28.062504893893514	22.122632945302716
48-49	21.872548769079327	28.117167171448955	28.036491814971924	21.973792244499798
50-51	21.908635754829067	28.12891569837187	27.97790383477613	21.984544712022934
52-53	22.00765232533838	27.949628108620416	27.69656452814272	22.346155037898484
54-55	21.90482229277387	27.71176078850263	27.924348806933335	22.459068111790167
56-57	21.971520207730258	27.436156724028816	28.19358686070438	22.39873620753655
58-59	21.803741673220134	27.212819859091695	28.323841794514827	22.65959667317334
60-61	21.828513484665198	27.01456628477905	28.648865011029674	22.508055219526078
62-63	21.61217372838009	27.58137564430465	28.604987825078247	22.201462802237014
64-65	21.228893127658583	27.794926056129853	28.403729884909705	22.572450931301862
66-67	21.271256118164473	28.41332210436506	27.923016931738776	22.392404845731694
68-69	21.393335976200223	28.705957370353886	27.44992162460046	22.45078502884543
70-71	21.390036867852682	28.339769699520943	27.419544544723447	22.85064888790293
72-73	21.28482067672386	27.901166327650113	27.389228734932225	23.4247842606938
74-75	21.728975205617406	27.5363233001189	27.435939748277715	23.298761745985967
76-77	22.146650829239263	27.382084183579835	27.336758528431226	23.13450645874967
78-79	22.369442373152896	27.24946151913906	26.647446839231076	23.733649268476963
80-81	22.27028411284414	27.674623353842808	26.582303768050085	23.472788765262965
82-83	22.18451944763057	27.809129093303785	26.64322743635012	23.36312402271552
84-85	22.18502946814747	27.811909184767398	26.6359063171681	23.367155029917036
86-87	22.568928915985563	28.11163421700712	26.554869173211255	22.76456769379606
88-89	22.716502433345624	27.771765841499352	26.910623309727715	22.60110841542731
90-91	22.42689350000069	27.790018554440415	27.188692921245938	22.594395024312952
92-93	22.227112215221087	27.25489117834803	27.090386221951036	23.427610384479856
94-95	22.053709030313083	27.200237509722403	27.779979434127167	22.966074025837347
96-97	21.633116100776775	27.511335272055724	28.077372313793443	22.778176313374058
98-99	21.895962045010528	27.61539725600879	28.406401685180004	22.082239013800677
100-101	22.578748293095714	30.52908551815446	25.742968983235333	21.149197205514493
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1443.0
1	1318.0
2	1832.0
3	2603.5
4	3408.0
5	5166.0
6	6666.5
7	7815.0
8	8599.0
9	8627.5
10	8170.0
11	7678.5
12	8393.5
13	10904.5
14	16942.0
15	25457.0
16	32416.0
17	36174.5
18	37074.0
19	35854.0
20	33577.5
21	31711.0
22	31672.0
23	35132.0
24	42388.0
25	53235.0
26	67463.5
27	83083.5
28	100400.5
29	119195.5
30	136725.0
31	154574.5
32	173163.0
33	190299.5
34	206782.0
35	222225.5
36	240192.5
37	257165.0
38	271081.5
39	282893.0
40	293432.0
41	304237.5
42	310875.0
43	315658.0
44	320225.5
45	324286.0
46	325899.5
47	325030.5
48	322722.5
49	316933.0
50	308432.0
51	297025.0
52	281562.5
53	264401.0
54	245952.0
55	225634.5
56	204573.0
57	182374.0
58	159798.5
59	137066.0
60	114343.0
61	92872.5
62	73310.5
63	56436.0
64	41863.5
65	31054.0
66	23589.5
67	17799.0
68	13514.5
69	9876.5
70	6889.5
71	4922.0
72	3523.0
73	2444.0
74	1642.5
75	1096.5
76	722.5
77	466.0
78	314.0
79	219.5
80	153.0
81	108.0
82	79.5
83	52.5
84	36.0
85	26.5
86	17.5
87	11.0
88	5.5
89	3.5
90	3.5
91	3.0
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.3064949653563263E-5
2	0.0044682127815186354
3	1.3064949653563263E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.3064949653563263E-5
10-11	1.3064949653563263E-5
12-13	6.5324748267816315E-6
14-15	6.5324748267816315E-6
16-17	0.0
18-19	5.617928351032203E-4
20-21	0.0011758891763647581
22-23	0.0029138844569025707
24-25	2.613939328115649E-5
26-27	0.008171542244585373
28-29	1.8312305247816175E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.03633950264696858
38-39	0.015973472578316977
40-41	0.02460488647558321
42-43	0.0012948667099952447
44-45	0.005286911933111378
46-47	0.004241480913404634
48-49	0.00233312853649513
50-51	0.002179794190981652
52-53	2.9644220650968734E-4
54-55	0.002360933756369308
56-57	0.01363404978636803
58-59	0.009350756223800247
60-61	0.003166483378274864
62-63	0.0010711487171376605
64-65	0.002854333346479245
66-67	2.4972787007099614E-4
68-69	6.764464469241534E-4
70-71	2.9347907554392206E-4
72-73	0.0
74-75	0.0
76-77	0.006137797776899015
78-79	3.7975365538605796E-4
80-81	0.0
82-83	9.76520619599078E-5
84-85	0.0015842839036755386
86-87	0.004954965888575204
88-89	0.07706554923461849
90-91	0.002695989818836397
92-93	0.0011877790730924049
94-95	0.0
96-97	0.01691957134633811
98-99	5.765895420495221E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	174.0
20-21	625.0
22-23	1223.0
24-25	2733.0
26-27	3242.0
28-29	5485.0
30-31	16991.0
32-33	22374.0
34-35	32011.0
36-37	49080.0
38-39	34938.0
40-41	56545.0
42-43	62558.0
44-45	73593.0
46-47	74436.0
48-49	68237.0
50-51	63108.0
52-53	10661.0
54-55	10267.0
56-57	10222.0
58-59	10733.0
60-61	72217.0
62-63	70235.0
64-65	75090.0
66-67	80676.0
68-69	81590.0
70-71	81685.0
72-73	79226.0
74-75	81191.0
76-77	81465.0
78-79	87021.0
80-81	88598.0
82-83	85328.0
84-85	84489.0
86-87	90207.0
88-89	94508.0
90-91	102588.0
92-93	106486.0
94-95	130245.0
96-97	167393.0
98-99	698118.0
100-101	4606475.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.57787345674889
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.20922722836356	79.8791016029348
2	6.406807730577811	11.221891933805281
3	1.372728336653971	3.606618856739246
4	0.4908262937043732	1.7194209215714664
5	0.20129012498578672	0.8814280547049198
6	0.10403385412988929	0.546663822732319
7	0.06081873470203435	0.37284628160740424
8	0.040976017334760126	0.2870873968724137
9	0.026147176692730117	0.20609227184824547
>10	0.0854020378562952	1.1575205867875356
>50	0.0014855682069329708	0.08354717801473488
>100	2.568967918814972E-4	0.037781092381594536
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3064949653563263E-5
2	0.0	2.6129899307126526E-5	0.0	0.0	1.3064949653563263E-5
3	0.0	2.6129899307126526E-5	0.0	0.0	1.3064949653563263E-5
4	0.0	9.145464757494284E-5	0.0	1.3064949653563263E-5	1.3064949653563263E-5
5	0.0	9.145464757494284E-5	0.0	1.3064949653563263E-5	1.3064949653563263E-5
6	1.3064949653563263E-5	9.145464757494284E-5	0.0	2.6129899307126526E-5	1.3064949653563263E-5
7	1.3064949653563263E-5	9.145464757494284E-5	0.0	2.6129899307126526E-5	1.3064949653563263E-5
8	1.3064949653563263E-5	9.145464757494284E-5	0.0	2.6129899307126526E-5	1.3064949653563263E-5
9	1.3064949653563263E-5	1.437144461891959E-4	0.0	7.838969792137958E-5	1.3064949653563263E-5
10-11	2.6129899307126526E-5	1.6331187066954077E-4	0.0	7.838969792137958E-5	1.3064949653563263E-5
12-13	2.6129899307126526E-5	1.7637682032310405E-4	0.0	9.145464757494284E-5	1.9597424480344895E-5
14-15	3.919484896068979E-5	2.2210414411057546E-4	0.0	1.045195972285061E-4	5.879227344103468E-5
16-17	5.879227344103468E-5	2.3516909376413873E-4	0.0	1.3064949653563264E-4	1.045195972285061E-4
18-19	7.185722309459794E-5	2.48234043417702E-4	0.0	1.7637682032310405E-4	1.1105207205528773E-4
20-21	7.838969792137958E-5	2.743639427248285E-4	0.0	2.286366189373571E-4	1.1758454688206937E-4
22-23	7.838969792137958E-5	3.2009126651229996E-4	0.0	4.2461086374080605E-4	1.24117021708851E-4
24-25	8.49221727481612E-5	3.396886909926448E-4	0.0	9.602737995368998E-4	1.3718197136241425E-4
26-27	9.145464757494284E-5	3.5928611547298973E-4	0.0	0.0027109770531143773	1.5677939584275916E-4
28-29	9.145464757494284E-5	3.9194848960689787E-4	0.0	0.01518147149744051	1.5677939584275916E-4
30-31	9.145464757494284E-5	4.5074076304793255E-4	0.0	0.02518269045724319	1.6331187066954077E-4
32-33	1.045195972285061E-4	4.7033818752827746E-4	0.0	0.0381235230890976	2.090391944570122E-4
34-35	1.437144461891959E-4	4.899356120086223E-4	0.0	0.05485319112048535	2.2210414411057546E-4
36-37	1.437144461891959E-4	5.160655113157489E-4	0.0	0.07674351426503061	2.2210414411057546E-4
38-39	1.437144461891959E-4	5.421954106228754E-4	0.0	0.12460695732085962	2.2210414411057546E-4
40-41	1.5677939584275916E-4	5.944552092371285E-4	0.0	0.4326458077777474	2.286366189373571E-4
42-43	1.698443454963224E-4	6.20585108544255E-4	0.0	0.47225220265252443	2.3516909376413873E-4
44-45	1.8290929514988569E-4	6.597799575049448E-4	0.0	0.5375377560713801	2.3516909376413873E-4
46-47	1.9597424480344893E-4	6.924423316388529E-4	0.0	0.6140983610412608	2.48234043417702E-4
48-49	1.9597424480344893E-4	6.924423316388529E-4	0.0	0.6775744189330979	2.612989930712652E-4
50-51	2.0250671963023057E-4	7.577670799066693E-4	0.0	0.7373792259722838	2.743639427248285E-4
52-53	2.090391944570122E-4	7.708320295602325E-4	0.0	0.9520690111544621	2.743639427248285E-4
54-55	2.090391944570122E-4	7.773645043870141E-4	0.0	1.6921722791295137	2.743639427248285E-4
56-57	2.1557166928379382E-4	7.96961928867359E-4	0.0	2.514970145936794	2.8089641755161014E-4
58-59	2.2210414411057546E-4	7.96961928867359E-4	0.0	3.370417321928329	2.874288923783918E-4
60-61	2.3516909376413873E-4	8.100268785209223E-4	0.0	4.01557106829611	3.2009126651229996E-4
62-63	2.3516909376413873E-4	8.100268785209223E-4	0.0	4.032111294557521	3.527536406462081E-4
64-65	2.3516909376413873E-4	8.622866771351753E-4	0.0	4.042791890899309	3.527536406462081E-4
66-67	2.612989930712652E-4	8.622866771351753E-4	0.0	4.051114263828628	3.527536406462081E-4
68-69	2.743639427248285E-4	8.884165764423018E-4	0.0	4.058175869116379	3.527536406462081E-4
70-71	2.8089641755161014E-4	9.080140009226467E-4	0.0	4.065289734202745	3.6581859029977137E-4
72-73	3.0049384203195505E-4	9.145464757494284E-4	0.0	4.070692090884493	3.788835399533346E-4
74-75	3.0049384203195505E-4	9.341439002297732E-4	0.0	4.075898473321438	3.8541601478011623E-4
76-77	3.0049384203195505E-4	9.798712240172446E-4	0.0	4.080575725297414	3.9194848960689787E-4
78-79	3.0049384203195505E-4	0.0010060011233243712	0.0	4.085154990150988	4.0501343926046114E-4
80-81	3.0049384203195505E-4	0.0010060011233243712	0.0	4.087643863059991	4.2461086374080605E-4
82-83	3.0049384203195505E-4	0.0010125335981511529	0.0	4.089296579191167	4.442082882211509E-4
84-85	3.0049384203195505E-4	0.001045195972285061	0.0	4.090942762847517	4.442082882211509E-4
86-87	3.0049384203195505E-4	0.001045195972285061	0.0	4.091262854114028	4.5074076304793255E-4
88-89	3.0049384203195505E-4	0.001136650619860004	0.0	4.091315113912643	4.572732378747142E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7990	0.0	46.9165	1
TATCTCG	9545	0.0	36.775978	94-95
GTATCAA	13940	0.0	34.84186	1
CCGTCTT	4070	0.0	34.48122	92-93
TATGCCG	4000	0.0	32.88131	88-89
TGCCGTC	4230	0.0	31.907026	90-91
ATGCCGT	4085	0.0	30.400406	88-89
GCCGTCT	4290	0.0	29.56892	90-91
CTCGTAT	4575	0.0	29.227222	84-85
CGTATGC	4570	0.0	28.980448	86-87
TCAACGC	16560	0.0	28.785845	4
ATCAACG	16745	0.0	28.467817	3
CAACGCA	17050	0.0	27.93347	5
TCGTATG	4550	0.0	27.213238	84-85
AACGCAG	17540	0.0	27.20191	6
TATCAAC	17985	0.0	26.69628	2
GCTATCT	15000	0.0	26.435198	94-95
TCTCGTA	4775	0.0	25.974783	82-83
CTATCTC	15310	0.0	25.581524	94-95
ACGCAGA	20460	0.0	23.340637	7
>>END_MODULE