FastQCFastQC Report
Fri 27 May 2016
SRR2139813_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139813_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6632964
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93800.051.4206851
GTATCAA168200.035.5196571
ATCAACG214600.027.4736023
TCAACGC214750.027.454414
CAACGCA219100.026.8716745
TATCAAC221550.026.7056772
AACGCAG224900.026.2153636
ACGCAGA260400.022.6097687
CGCAGAG262200.022.4702858
GTACATG266900.020.9003871
TACATGG267000.020.4598732
ACATGGG270650.019.8022353
CTTATAC50950.019.4353891
GCAGAGT308500.019.0043059
GAGTACT154100.018.42053212-13
CAGAGTA299350.017.6556210-11
AGAGTAC284050.017.61891210-11
CATGGGG159450.016.456814
TATGCCG30250.016.35487742-43
GTACTTT174900.016.32539714-15
Produced by FastQC (version 0.11.5)