##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139813_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6632964
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.052159185546614	33.0	31.0	34.0	30.0	34.0
2	32.1992851159753	33.0	31.0	34.0	30.0	34.0
3	32.24732970056825	34.0	31.0	34.0	30.0	34.0
4	35.900962978240194	37.0	35.0	37.0	35.0	37.0
5	35.78445789845988	37.0	35.0	37.0	35.0	37.0
6	35.8028440980533	37.0	35.0	37.0	35.0	37.0
7	35.72003300485273	37.0	35.0	37.0	33.0	37.0
8	35.69959040935546	37.0	35.0	37.0	33.0	37.0
9	37.331066774974204	39.0	37.0	39.0	34.0	39.0
10-11	37.24045818732018	39.0	37.0	39.0	33.0	39.0
12-13	37.21760905079539	39.0	37.0	39.0	33.0	39.0
14-15	38.4178710302061	40.0	38.0	41.0	33.0	41.0
16-17	38.40235782977263	40.0	38.0	41.0	33.0	41.0
18-19	38.44939268477863	40.0	38.0	41.0	33.5	41.0
20-21	38.429200930977416	40.0	38.0	41.0	34.0	41.0
22-23	38.36001012254137	40.0	38.0	41.0	34.0	41.0
24-25	38.27275067332781	40.0	38.0	41.0	33.5	41.0
26-27	38.12870380854145	40.0	38.0	41.0	33.0	41.0
28-29	38.03408564276746	40.0	37.0	41.0	33.0	41.0
30-31	37.91607801842537	40.0	37.0	41.0	33.0	41.0
32-33	38.07492985696558	40.0	37.0	41.0	33.0	41.0
34-35	38.05915028235458	40.0	37.0	41.0	33.0	41.0
36-37	37.98213599925911	40.0	37.0	41.0	33.0	41.0
38-39	37.853402601777304	40.0	37.0	41.0	33.0	41.0
40-41	37.813346610818115	40.0	37.0	41.0	33.0	41.0
42-43	37.73586955541607	40.0	37.0	41.0	33.0	41.0
44-45	37.73645398730247	40.0	37.0	41.0	33.0	41.0
46-47	37.77005088357919	40.0	37.0	41.0	33.0	41.0
48-49	37.698851200079886	40.0	37.0	41.0	32.0	41.0
50-51	37.632412594874	40.0	36.5	41.0	32.0	41.0
52-53	37.502989614232135	39.5	36.0	41.0	32.0	41.0
54-55	37.37862141322939	39.0	36.0	41.0	32.0	41.0
56-57	37.208147663741485	39.0	36.0	41.0	31.0	41.0
58-59	37.05141859334085	39.0	35.0	40.5	31.0	41.0
60-61	36.89171014856461	39.0	35.0	40.0	31.0	41.0
62-63	36.66662674772981	38.0	35.0	40.0	31.0	41.0
64-65	36.450165772346466	38.0	35.0	40.0	31.0	41.0
66-67	36.191880588321375	37.5	35.0	40.0	30.5	41.0
68-69	35.90408803130202	37.0	34.0	39.5	30.0	41.0
70-71	35.61595863077466	36.5	34.0	39.0	30.0	41.0
72-73	35.30205047297703	36.0	34.0	39.0	30.0	40.0
74-75	34.96598727598556	35.5	34.0	38.5	29.0	40.0
76-77	33.88635049867578	35.0	32.5	37.0	28.5	39.0
78-79	34.24554334189533	35.0	33.0	37.0	29.0	39.0
80-81	34.13744722932178	35.0	33.0	36.5	29.0	39.0
82-83	33.894213740821826	35.0	33.0	36.0	29.0	37.5
84-85	33.68501024964003	35.0	33.0	36.0	29.0	37.0
86-87	33.504367728409164	35.0	33.0	35.0	29.0	37.0
88-89	33.3000206775292	35.0	33.0	35.0	29.0	36.0
90-91	33.18442402872428	35.0	33.0	35.0	29.0	36.0
92-93	33.11809645448196	35.0	33.0	35.0	29.0	36.0
94-95	33.087658563835205	34.5	33.0	35.0	29.0	35.5
96-97	33.04415333721511	34.0	33.0	35.0	29.0	35.0
98-99	33.12342337316289	34.0	33.0	35.0	29.0	35.0
100-101	32.57367219824658	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	38.0
21	268.0
22	1129.0
23	3724.0
24	9724.0
25	20552.0
26	37091.0
27	59098.0
28	85311.0
29	113911.0
30	146703.0
31	184967.0
32	232384.0
33	297168.0
34	393655.0
35	538198.0
36	814148.0
37	1328374.0
38	1554063.0
39	780983.0
40	31470.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.491962266039735	22.12947333952061	15.366569153699613	24.011995240740035
2	14.057913809854353	22.943456977597606	40.91114547293373	22.08748373961431
3	17.113917699538245	28.190202750987343	31.462646261912475	23.23323328756194
4	12.152636438249928	19.827184347751622	39.115816096695234	28.904363117303216
5	12.566041365519245	38.68104817092329	35.848061289040615	12.904849174516853
6	28.216359986274615	39.31171645134815	19.415181508598568	13.056742053778672
7	25.02603662555684	33.256550163697554	24.342465902121585	17.374947308624016
8	23.954976990678677	37.01774953097891	21.8594130768688	17.167860401473607
9	25.685108497498256	16.43872935236796	21.579975407675967	36.29618674245782
10-11	23.728788517471227	27.29495441253714	29.399021312342416	19.57723575764922
12-13	24.80206435614606	24.970495844693264	29.325434300563067	20.90200549859761
14-15	22.00614989015469	26.059556481838285	27.047395402718905	24.88689822528812
16-17	21.318274906964668	29.06179650605672	27.69890655218391	21.921022034794703
18-19	21.41737834396364	28.161246280296982	29.46383207883343	20.957543296905946
20-21	22.484977644564022	27.2690560208907	29.0951457306055	21.150820603939774
22-23	22.123195717302195	27.382861714703093	28.930971383818004	21.562971184176707
24-25	21.779971007183654	27.654914903731303	29.024947636777803	21.54016645230724
26-27	21.733164532094477	27.66733129451091	29.015022741920678	21.584481431473936
28-29	21.588751728283263	27.666013510142268	29.1519087486063	21.593326012968173
30-31	21.866074497220218	27.578759088153987	28.90095137587711	21.654215038748685
32-33	21.733330220551696	27.72471984207282	28.961770089341886	21.580179848033595
34-35	21.85507202714016	27.825448950458192	28.89284652438655	21.426632498015096
36-37	21.764485457247755	27.722193446125598	28.799285846065576	21.71403525056107
38-39	21.665567152836125	27.752430982271175	28.92191878314402	21.66008308174868
40-41	21.799513952371566	27.68046613506845	28.874892890613758	21.645127021946227
42-43	21.848637928900512	27.752014096001837	28.588956351776794	21.810391623320854
44-45	21.858030683117757	27.943559911587922	28.374621606990313	21.82378779830401
46-47	22.063132831525795	27.851114788977394	28.0608684635479	22.024883915948912
48-49	21.961241322824016	27.976922548130695	27.962098818565046	22.099737310480247
50-51	21.911344882214642	28.067617530987288	27.89813046006117	22.122907126736898
52-53	22.067279234548227	27.996258657162628	27.909018933992023	22.027443174297122
54-55	22.076361824454974	27.934511386502226	27.827293534549007	22.16183325449379
56-57	22.151625138678416	28.044254532502134	27.717622446604288	22.086497882215163
58-59	22.011910126105697	28.11809841189703	27.801425527680447	22.068565934316826
60-61	22.1680827147228	28.162694875621032	27.664657349169904	22.004565060486268
62-63	22.041165335543816	28.240994321127346	27.67977488695156	22.038065456377282
64-65	22.061335933324738	28.137346703525708	27.681764127306362	22.119553235843192
66-67	22.156288955705826	28.086387937732827	27.563796096698283	22.193527009863057
68-69	22.143962097662364	28.045015087316884	27.62889241387519	22.182130401145564
70-71	22.145852682045817	27.8403661615997	27.726718179635885	22.287062976718598
72-73	22.160848973599286	27.854841011922876	27.678578846047824	22.30573116843001
74-75	22.098605734332256	27.92940432326821	27.675601441370013	22.296388501029522
76-77	22.188586968959843	27.867823410782773	27.643588029866763	22.30000159039063
78-79	22.143205995287225	27.855893944364468	27.606946105074915	22.393953955273393
80-81	22.170218555924897	27.876734594221887	27.61229844860798	22.340748401245236
82-83	22.189928587630483	27.80168338943109	27.72026933013036	22.28811869280807
84-85	22.242360782385468	27.805490909518056	27.640256255908046	22.31189205218843
86-87	22.165708945578906	27.818750542871406	27.71619363163441	22.299346879915273
88-89	22.184710681847996	27.72879330231417	27.68276076797132	22.40373524786651
90-91	22.234080753055473	27.71512380197838	27.738007756707155	22.312787688258993
92-93	22.253864391377142	27.666841108673246	27.750279342942967	22.329015157006644
94-95	22.057473702261312	27.653296299137626	27.81048479898194	22.47874519961913
96-97	22.10701299663479	27.68572615962002	27.858119394933546	22.34914144881164
98-99	22.28662135358715	28.310507474239273	27.945237288955543	21.457633883218037
100-101	26.132716159981058	35.45378911928157	28.75108085833005	9.66241386240732
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1766.0
1	1580.0
2	2136.0
3	3049.5
4	4131.0
5	6425.5
6	8201.0
7	9476.5
8	10142.0
9	9790.5
10	9129.5
11	8583.5
12	9622.0
13	13049.5
14	20795.0
15	31418.0
16	39767.5
17	43652.5
18	43816.0
19	41282.5
20	37031.0
21	33143.0
22	31833.0
23	34455.0
24	41000.5
25	51170.0
26	64297.5
27	78994.0
28	95228.0
29	112515.0
30	129142.5
31	144942.0
32	161501.5
33	178638.5
34	193982.0
35	208340.5
36	224208.5
37	237346.5
38	246422.0
39	253719.5
40	260296.0
41	266005.0
42	268664.5
43	272032.5
44	275749.5
45	277474.0
46	277832.5
47	277290.5
48	280070.5
49	278034.0
50	268636.5
51	259688.5
52	248624.0
53	235266.5
54	221594.0
55	205439.5
56	186980.5
57	167896.0
58	148371.5
59	128358.5
60	107844.5
61	87604.5
62	69231.5
63	53596.5
64	40104.0
65	29596.0
66	21893.0
67	16308.5
68	12016.5
69	8487.5
70	5997.5
71	4303.0
72	2987.5
73	2067.0
74	1433.0
75	975.5
76	668.5
77	449.5
78	311.0
79	221.5
80	158.0
81	116.0
82	88.5
83	67.0
84	45.0
85	30.5
86	22.5
87	17.0
88	13.0
89	9.0
90	5.5
91	4.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00422134056509277
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	6.332010847639155E-4
20-21	0.0012513761368299898
22-23	0.0027970108925103434
24-25	3.0163137328239786E-5
26-27	0.007898260149830071
28-29	2.4909409008419834E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.034460770409725935
38-39	0.014768558564005621
40-41	0.023402752421986483
42-43	0.001083764470022682
44-45	0.005034530606650615
46-47	0.0040700659439162614
48-49	0.002207220902821765
50-51	0.002174976353311068
52-53	2.081294531898128E-4
54-55	0.002288159154928985
56-57	0.013760622357973271
58-59	0.009493426620464176
60-61	0.0031577232152670506
62-63	9.361370815555561E-4
64-65	0.002655110529033008
66-67	2.9891805252171345E-4
68-69	4.4082543092522643E-4
70-71	1.489852521361226E-4
72-73	0.0
74-75	9.566275587754365E-6
76-77	0.005963609184035719
78-79	2.8526421466663334E-4
80-81	0.0
82-83	1.1163720425528546E-4
84-85	0.0017619498582042517
86-87	0.0055461375860697765
88-89	0.07512041611177576
90-91	0.0028820816864295125
92-93	0.0015580198297250073
94-95	0.0
96-97	0.0165975603206732
98-99	3.628270175026544E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	160.0
20-21	518.0
22-23	1068.0
24-25	2428.0
26-27	3492.0
28-29	6366.0
30-31	17355.0
32-33	22469.0
34-35	31539.0
36-37	49890.0
38-39	57132.0
40-41	58275.0
42-43	66708.0
44-45	79214.0
46-47	79083.0
48-49	71678.0
50-51	64589.0
52-53	61211.0
54-55	66682.0
56-57	71275.0
58-59	71727.0
60-61	71819.0
62-63	68229.0
64-65	70539.0
66-67	76661.0
68-69	74439.0
70-71	74488.0
72-73	69907.0
74-75	70302.0
76-77	71587.0
78-79	77434.0
80-81	79010.0
82-83	74962.0
84-85	73718.0
86-87	77647.0
88-89	82686.0
90-91	89377.0
92-93	90783.0
94-95	107809.0
96-97	137912.0
98-99	517252.0
100-101	3693544.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.85131623738644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.88927924416109	81.64483408948469
2	6.037998013246302	10.729681418313165
3	1.2551779499011366	3.3457263888258084
4	0.41234567246420134	1.465498229729381
5	0.17453944071419092	0.775402952139657
6	0.0769479237089912	0.4102154581966718
7	0.05029290110171071	0.31280133221985895
8	0.02397088485543821	0.17038757366244142
9	0.018282909618253795	0.146201452580792
>10	0.05915574563828778	0.8419136886680931
>50	0.0016052415273223291	0.09759366919971048
>100	4.040730630975418E-4	0.05974374697965162
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.507621630390275E-5
2	0.0	0.0	0.0	0.0	1.507621630390275E-5
3	0.0	1.507621630390275E-5	0.0	0.0	1.507621630390275E-5
4	0.0	7.538108151951374E-5	0.0	1.507621630390275E-5	1.507621630390275E-5
5	0.0	7.538108151951374E-5	0.0	1.507621630390275E-5	1.507621630390275E-5
6	1.507621630390275E-5	7.538108151951374E-5	0.0	1.507621630390275E-5	1.507621630390275E-5
7	1.507621630390275E-5	7.538108151951374E-5	0.0	1.507621630390275E-5	1.507621630390275E-5
8	1.507621630390275E-5	7.538108151951374E-5	0.0	1.507621630390275E-5	1.507621630390275E-5
9	1.507621630390275E-5	1.507621630390275E-4	0.0	1.507621630390275E-5	1.507621630390275E-5
10-11	3.01524326078055E-5	1.507621630390275E-4	0.0	9.04572978234165E-5	1.507621630390275E-5
12-13	3.01524326078055E-5	1.5830027119097885E-4	0.0	1.20609730431222E-4	2.2614324455854125E-5
14-15	3.01524326078055E-5	1.9599081195073576E-4	0.0	1.20609730431222E-4	7.538108151951376E-5
16-17	4.522864891170825E-5	2.0352892010268712E-4	0.0	1.3568594673512476E-4	1.0553351412731924E-4
18-19	4.522864891170825E-5	2.110670282546385E-4	0.0	1.80914595646833E-4	1.20609730431222E-4
20-21	4.522864891170825E-5	2.110670282546385E-4	0.0	2.110670282546385E-4	1.20609730431222E-4
22-23	4.522864891170825E-5	2.110670282546385E-4	0.0	2.110670282546385E-4	1.4322405488707612E-4
24-25	4.522864891170825E-5	2.110670282546385E-4	0.0	2.41219460862444E-4	1.6583837934293024E-4
26-27	7.538108151951374E-5	2.110670282546385E-4	0.0	3.166005423819577E-4	1.6583837934293024E-4
28-29	7.538108151951374E-5	2.110670282546385E-4	0.0	6.332010847639155E-4	1.6583837934293024E-4
30-31	7.538108151951374E-5	2.5629567716634673E-4	0.0	0.0016433075771253997	1.6583837934293024E-4
32-33	7.538108151951374E-5	2.5629567716634673E-4	0.0	0.0039047400227108127	1.80914595646833E-4
34-35	9.045729782341649E-5	2.8644810977415224E-4	0.0	0.006641073281869162	2.5629567716634673E-4
36-37	1.0553351412731924E-4	3.1660054238195776E-4	0.0	0.010952871144785348	2.5629567716634673E-4
38-39	1.0553351412731924E-4	3.1660054238195776E-4	0.0	0.017224577127208894	2.5629567716634673E-4
40-41	1.507621630390275E-4	3.316767586858605E-4	0.0	0.02418225095146001	2.5629567716634673E-4
42-43	1.507621630390275E-4	3.392148668378119E-4	0.0	0.03009212774258989	2.638337853182981E-4
44-45	1.7337648749488164E-4	3.693672994456174E-4	0.0	0.037487011839654194	2.9398621792610364E-4
46-47	2.110670282546385E-4	3.919816239014715E-4	0.0	0.045733702157888995	3.392148668378119E-4
48-49	2.2614324455854124E-4	4.0705784020537424E-4	0.0	0.0537316349071094	3.7690540759756873E-4
50-51	2.7137189347024946E-4	4.598245972690339E-4	0.0	0.06178233441339347	3.7690540759756873E-4
52-53	2.8644810977415224E-4	4.6736270542098527E-4	0.0	0.06895861337405118	4.0705784020537424E-4
54-55	3.01524326078055E-4	4.6736270542098527E-4	0.0	0.07663994558088963	4.447483809651311E-4
56-57	3.01524326078055E-4	4.6736270542098527E-4	0.0	0.0849394026561881	4.598245972690339E-4
58-59	3.0906243423000637E-4	4.7490081357293666E-4	0.0	0.09257550621411484	4.7490081357293666E-4
60-61	3.316767586858605E-4	4.82438921724888E-4	0.0	0.10052821031442354	4.975151380287908E-4
62-63	3.7690540759756873E-4	4.82438921724888E-4	0.0	0.10845830009027638	5.125913543326935E-4
64-65	3.7690540759756873E-4	5.125913543326935E-4	0.0	0.1161773228378746	5.125913543326935E-4
66-67	3.844435157495201E-4	5.201294624846448E-4	0.0	0.12430340342567817	5.125913543326935E-4
68-69	4.22134056509277E-4	5.276675706365962E-4	0.0	0.13190181644284515	5.125913543326935E-4
70-71	4.22134056509277E-4	5.276675706365962E-4	0.0	0.13985452054315387	5.276675706365962E-4
72-73	4.82438921724888E-4	5.276675706365962E-4	0.0	0.14636744598643986	6.0304865215611E-4
74-75	4.975151380287908E-4	5.276675706365962E-4	0.0	0.15177226953138898	6.0304865215611E-4
76-77	4.975151380287908E-4	5.276675706365962E-4	0.0	0.15698110226438738	6.0304865215611E-4
78-79	4.975151380287908E-4	5.42743786940499E-4	0.0	0.16157181012892577	6.0304865215611E-4
80-81	4.975151380287908E-4	5.502818950924504E-4	0.0	0.16454936284894656	6.0304865215611E-4
82-83	5.050532461807421E-4	5.578200032444017E-4	0.0	0.16639619934617467	6.0304865215611E-4
84-85	5.276675706365962E-4	5.65358111396353E-4	0.0	0.16794151151732467	6.0304865215611E-4
86-87	5.276675706365962E-4	5.728962195483045E-4	0.0	0.16825811205970664	6.181248684600127E-4
88-89	5.276675706365962E-4	6.0304865215611E-4	0.0	0.1683108788167703	6.407391929158669E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9380	0.0	51.420685	1
GTATCAA	16820	0.0	35.519657	1
ATCAACG	21460	0.0	27.473602	3
TCAACGC	21475	0.0	27.45441	4
CAACGCA	21910	0.0	26.871674	5
TATCAAC	22155	0.0	26.705677	2
AACGCAG	22490	0.0	26.215363	6
ACGCAGA	26040	0.0	22.609768	7
CGCAGAG	26220	0.0	22.470285	8
GTACATG	26690	0.0	20.900387	1
TACATGG	26700	0.0	20.459873	2
ACATGGG	27065	0.0	19.802235	3
CTTATAC	5095	0.0	19.435389	1
GCAGAGT	30850	0.0	19.004305	9
GAGTACT	15410	0.0	18.420532	12-13
CAGAGTA	29935	0.0	17.65562	10-11
AGAGTAC	28405	0.0	17.618912	10-11
CATGGGG	15945	0.0	16.45681	4
TATGCCG	3025	0.0	16.354877	42-43
GTACTTT	17490	0.0	16.325397	14-15
>>END_MODULE