FastQCFastQC Report
Fri 27 May 2016
SRR2139812_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139812_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7588404
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82500.045.2117961
GTATCAA143150.033.3720051
TCAACGC172150.026.9737914
ATCAACG173100.026.8257583
CAACGCA177150.026.2124675
TATCAAC185250.025.382472
AACGCAG184000.025.372676
ACGCAGA209400.022.2352187
CGCAGAG212800.021.899568
GTACATG201800.018.834991
TACATGG203350.018.5272852
GCAGAGT258200.018.0973789
ACATGGG203050.018.019823
AGAGTAC230300.017.83510410-11
GAGTACT145100.017.30949612-13
CAGAGTA253750.016.48284510-11
CATGGGG144450.016.2031384
AGTACTT158950.015.74874812-13
CTTATAC57650.015.7046611
GTACTTT166850.015.05412914-15
Produced by FastQC (version 0.11.5)