##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139812_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7588404
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.080822924029874	33.0	31.0	34.0	30.0	34.0
2	32.242132864829024	34.0	31.0	34.0	30.0	34.0
3	32.297762480753526	34.0	31.0	34.0	30.0	34.0
4	35.935688980186086	37.0	35.0	37.0	35.0	37.0
5	35.818746339810055	37.0	35.0	37.0	35.0	37.0
6	35.82893926048218	37.0	35.0	37.0	35.0	37.0
7	35.749897475147606	37.0	35.0	37.0	33.0	37.0
8	35.73015498384113	37.0	35.0	37.0	33.0	37.0
9	37.37366249872832	39.0	37.0	39.0	34.0	39.0
10-11	37.28745412078746	39.0	37.0	39.0	34.0	39.0
12-13	37.26525162603362	39.0	37.0	39.0	33.5	39.0
14-15	38.49504296292079	40.0	38.0	41.0	34.0	41.0
16-17	38.477024154222676	40.0	38.0	41.0	34.0	41.0
18-19	38.512851846053536	40.0	38.0	41.0	34.0	41.0
20-21	38.488277883280446	40.0	38.0	41.0	34.0	41.0
22-23	38.42557609411402	40.0	38.0	41.0	34.0	41.0
24-25	38.34336695151724	40.0	38.0	41.0	34.0	41.0
26-27	38.20395087969537	40.0	38.0	41.0	33.0	41.0
28-29	38.112286706537745	40.0	37.5	41.0	33.0	41.0
30-31	38.00408398248373	40.0	37.0	41.0	33.0	41.0
32-33	38.16384178851176	40.0	38.0	41.0	33.5	41.0
34-35	38.15087750214187	40.0	38.0	41.0	33.0	41.0
36-37	38.0719714512406	40.0	37.0	41.0	33.0	41.0
38-39	37.94538606921466	40.0	37.0	41.0	33.0	41.0
40-41	37.90636613701397	40.0	37.0	41.0	33.0	41.0
42-43	37.83722659770454	40.0	37.0	41.0	33.0	41.0
44-45	37.83660503633861	40.0	37.0	41.0	33.0	41.0
46-47	37.87546568248865	40.0	37.0	41.0	33.0	41.0
48-49	37.8085618493233	40.0	37.0	41.0	33.0	41.0
50-51	37.746058731670715	40.0	37.0	41.0	32.5	41.0
52-53	37.61881777134753	40.0	36.5	41.0	32.0	41.0
54-55	37.506102934301595	39.5	36.0	41.0	32.0	41.0
56-57	37.34745500713231	39.0	36.0	41.0	31.5	41.0
58-59	37.19278226398852	39.0	35.5	41.0	31.0	41.0
60-61	37.04996044021038	39.0	35.0	40.0	31.0	41.0
62-63	36.837811262598706	38.5	35.0	40.0	31.0	41.0
64-65	36.62773480056743	38.0	35.0	40.0	31.0	41.0
66-67	36.38099055470321	38.0	35.0	40.0	31.0	41.0
68-69	36.09744123451137	37.0	34.5	40.0	30.0	41.0
70-71	35.820539796142704	37.0	34.0	39.0	30.0	41.0
72-73	35.502848788966816	36.0	34.0	39.0	30.0	40.5
74-75	35.16147809382868	36.0	34.0	39.0	30.0	40.0
76-77	34.075713307340706	35.0	32.5	37.0	28.5	39.0
78-79	34.418818720339004	35.0	33.5	37.0	29.0	39.0
80-81	34.29807801147791	35.0	34.0	37.0	29.0	39.0
82-83	34.038230509890525	35.0	33.0	36.0	29.0	38.5
84-85	33.81356443496904	35.0	33.0	36.0	29.0	37.0
86-87	33.61454749117969	35.0	33.0	35.5	29.0	37.0
88-89	33.40002351677679	35.0	33.0	35.0	29.0	36.5
90-91	33.27347549669439	35.0	33.0	35.0	29.0	36.0
92-93	33.1997729125965	35.0	33.0	35.0	29.0	36.0
94-95	33.16044146049408	35.0	33.0	35.0	29.0	36.0
96-97	33.1093167897729	34.0	33.0	35.0	29.0	35.0
98-99	33.1819185171579	34.0	33.0	35.0	29.0	35.0
100-101	32.639725973132585	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	1.0
18	1.0
19	11.0
20	45.0
21	249.0
22	1160.0
23	4180.0
24	10585.0
25	22389.0
26	40643.0
27	64490.0
28	93284.0
29	125792.0
30	161903.0
31	205059.0
32	257503.0
33	328629.0
34	435686.0
35	597554.0
36	898795.0
37	1484530.0
38	1860682.0
39	955678.0
40	39554.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.870558815793146	22.478455285195675	15.52280295039642	24.128182948614754
2	13.671448448400694	22.931203122360177	41.47152135196846	21.925827077270664
3	16.776241749912103	28.14765792648889	31.806516890771764	23.269583432827247
4	12.015359224416624	19.916730843534424	39.78330621300606	28.2846037190429
5	12.450786753051103	38.58047093960732	36.49072980299942	12.478012504342152
6	28.079343166231002	39.8969796547469	19.448200174898435	12.57547700412366
7	24.579120985124145	33.3260986104588	24.90449902245584	17.190281381961213
8	23.21536913427382	37.771657913838006	22.198752201385165	16.814220750503004
9	25.00134415616248	16.895160036286946	22.02459173233265	36.07890407521793
10-11	23.350021954550655	27.45809263713424	30.03217804428968	19.159707364025426
12-13	24.283136480345537	25.000250382030266	29.883846458359358	20.832766679264836
14-15	21.520632006413997	26.335821076474055	27.317621729154112	24.825925187957836
16-17	21.16244733411663	29.368085832014216	28.00630409240204	21.463162741467112
18-19	21.189873622899174	28.635012793606734	29.502670570276674	20.672443013217414
20-21	22.01728592774243	27.86568752699979	29.202728985234888	20.914297560022888
22-23	21.71391858732831	27.850513088072265	29.05326528624445	21.382303038354976
24-25	21.535474356193237	28.04899197961792	29.100045198155716	21.315488466033127
26-27	21.553357952774277	28.140279507306072	28.995165292932285	21.31119724698737
28-29	21.408962828278643	28.191627104583304	29.069024902653755	21.330385164484294
30-31	21.794242177665897	28.005106397931918	28.970690393481558	21.22996103092063
32-33	21.46560358787107	28.249703705832502	28.900339077866597	21.38435362842983
34-35	21.62182471924685	28.23052411529395	28.976942366114162	21.17070879934504
36-37	21.532989574617627	28.23733993436994	28.91262448250161	21.317046008510825
38-39	21.400568610940194	28.19764596012726	29.004977952953514	21.39680747597903
40-41	21.61086957539144	28.091631023012276	28.912222432410562	21.385276969185718
42-43	21.61380709299017	28.20908700457401	28.66327993346902	21.513825968966803
44-45	21.620226977588295	28.307229968905578	28.54341022880887	21.529132824697257
46-47	21.767478948568773	28.20506782742075	28.276876629445617	21.750576594564862
48-49	21.674049625266072	28.319588414747226	28.284723037141056	21.72163892284564
50-51	21.606410911168666	28.421230325916774	28.225097101221174	21.747261661693386
52-53	21.798349256734586	28.3273413244241	28.182521330790237	21.691788088051076
54-55	21.739056396660267	28.267172930487988	28.184263205955233	21.80950746689651
56-57	21.85544935374209	28.39121885599571	28.031240863152597	21.722090927109605
58-59	21.736463204688928	28.459091123874643	28.126954975884523	21.67749069555191
60-61	21.831571974829135	28.490056604341675	28.03292385511697	21.64544756571222
62-63	21.76803167955625	28.58245557671569	27.97940813617672	21.67010460755134
64-65	21.756809279203008	28.494406996236222	27.998087775095986	21.75069594946478
66-67	21.82347976580532	28.490986672453257	27.879809516505027	21.8057240452364
68-69	21.81008642052304	28.390140465914538	27.965450737459868	21.83432237610256
70-71	21.825592007312547	28.193651323951585	28.045813921744532	21.934942746991336
72-73	21.838448444007117	28.24709470335392	28.006819157810366	21.907637694828598
74-75	21.69967604861132	28.330689013472053	27.991766218456434	21.977868719460197
76-77	21.83494690512158	28.305311082532196	27.910508975281967	21.949233037064257
78-79	21.783314409474592	28.300016230071023	27.929039570394735	21.987629790059653
80-81	21.83124945343842	28.265688366983312	27.94756390671287	21.955498272865405
82-83	21.830441478470565	28.199157501497712	28.05301280871918	21.917388211312538
84-85	21.926033241481832	28.18561710099431	28.00877695381506	21.8795727037088
86-87	21.776277221606687	28.202752799320468	28.06707467052242	21.953895308550425
88-89	21.824450404702876	28.139010737988773	28.041334939464857	21.99520391784349
90-91	21.868859822513837	28.13039339914325	28.102043480569748	21.89870329777316
92-93	21.882500682275065	28.07203622622344	28.085813205201482	21.95964988630001
94-95	21.75139849587766	28.076037260276493	28.153788143114877	22.01877610073097
96-97	21.66949232497273	28.13233525368141	28.233750681216165	21.96442174012969
98-99	21.920092355044414	28.709597869719595	28.320584620217236	21.04972515501876
100-101	25.651467379523083	35.85889190577992	29.133872629578327	9.355768085118669
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1353.0
1	1258.5
2	1871.0
3	2736.5
4	3675.0
5	5665.0
6	7229.5
7	8333.0
8	9025.0
9	8853.5
10	8238.5
11	7647.5
12	8385.5
13	11073.0
14	17613.5
15	26964.5
16	34872.0
17	39361.0
18	40260.0
19	38927.5
20	37027.5
21	35645.5
22	36245.5
23	40617.5
24	49255.0
25	62257.5
26	78824.5
27	96980.5
28	116686.5
29	137937.0
30	157979.0
31	178168.0
32	199342.0
33	218441.0
34	236924.0
35	254275.0
36	271434.0
37	286189.5
38	296939.5
39	305842.0
40	314008.5
41	320483.0
42	322801.5
43	324343.0
44	325492.0
45	324867.5
46	324562.0
47	324523.0
48	318577.0
49	308092.5
50	297246.0
51	282923.0
52	264924.5
53	245562.5
54	225810.5
55	205982.5
56	187070.5
57	167349.0
58	146519.0
59	125881.0
60	104883.0
61	84717.5
62	66542.5
63	50990.5
64	38200.0
65	28380.5
66	21422.5
67	16500.5
68	12513.5
69	8876.0
70	6166.5
71	4496.5
72	3195.0
73	2153.5
74	1423.0
75	948.5
76	633.0
77	419.5
78	281.5
79	196.0
80	145.5
81	104.0
82	70.5
83	49.0
84	34.0
85	22.5
86	15.5
87	12.5
88	10.5
89	7.0
90	4.5
91	4.5
92	4.5
93	2.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003927044474701136
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.732430692936222E-4
20-21	0.0013244382915536747
22-23	0.0028468568920626074
24-25	1.3183068326195251E-5
26-27	0.008288738775748667
28-29	2.5070896206027876E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.03531682005455844
38-39	0.015190255256161321
40-41	0.024249054059726317
42-43	0.0014977452778450852
44-45	0.0054776451292751875
46-47	0.004527585572764728
48-49	0.0025433063250686005
50-51	0.0022126775718361836
52-53	2.594300480385179E-4
54-55	0.002546973097123652
56-57	0.014315256974310808
58-59	0.009665348290342003
60-61	0.0034548530863241335
62-63	0.0011952395663579498
64-65	0.0029410872506639386
66-67	2.883932249416491E-4
68-69	4.420871375852537E-4
70-71	2.638804304993321E-4
72-73	0.0
74-75	0.0
76-77	0.005680761155553193
78-79	2.946321639494987E-4
80-81	0.0
82-83	1.386570115167647E-4
84-85	0.0017845451009929456
86-87	0.0053793733948859735
88-89	0.07773368911200883
90-91	0.0030054620123430454
92-93	0.001371105215984758
94-95	0.0
96-97	0.016981500078968884
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	162.0
20-21	676.0
22-23	1280.0
24-25	2878.0
26-27	3641.0
28-29	6116.0
30-31	17621.0
32-33	23379.0
34-35	32987.0
36-37	52424.0
38-39	59084.0
40-41	60985.0
42-43	69007.0
44-45	81379.0
46-47	80590.0
48-49	73934.0
50-51	67659.0
52-53	66003.0
54-55	72630.0
56-57	76731.0
58-59	77058.0
60-61	76379.0
62-63	73024.0
64-65	76903.0
66-67	82340.0
68-69	80379.0
70-71	79834.0
72-73	75836.0
74-75	77735.0
76-77	79330.0
78-79	84225.0
80-81	85892.0
82-83	82501.0
84-85	81755.0
86-87	86670.0
88-89	91851.0
90-91	98991.0
92-93	100478.0
94-95	121626.0
96-97	158314.0
98-99	598250.0
100-101	4369867.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.7464873369632
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.27339654170422	80.08919933848269
2	6.360331814033354	11.161935499579238
3	1.3900687707330432	3.6592095536590494
4	0.4661042361720695	1.6359603782790957
5	0.2026172892328046	0.8889477701958048
6	0.10426787383520503	0.5489483802679735
7	0.060383016271555984	0.3708878300847811
8	0.035005196306834	0.24572664115724163
9	0.023389611046532165	0.18471205885599262
>10	0.08321350767670155	1.1127409430578712
>50	9.91164669061447E-4	0.055585099672278135
>100	2.0904311398168898E-4	0.03621486293202984
>500	2.193520475056736E-5	0.009931643775937464
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.6356003185913664E-5	0.0	1.3178001592956832E-5	0.0
3	0.0	2.6356003185913664E-5	0.0	1.3178001592956832E-5	0.0
4	0.0	1.0542401274365465E-4	0.0	2.6356003185913664E-5	0.0
5	0.0	1.0542401274365465E-4	0.0	2.6356003185913664E-5	0.0
6	0.0	1.0542401274365465E-4	0.0	2.6356003185913664E-5	0.0
7	0.0	1.0542401274365465E-4	0.0	3.95340047788705E-5	0.0
8	2.6356003185913664E-5	1.0542401274365465E-4	0.0	5.271200637182733E-5	0.0
9	3.95340047788705E-5	1.7131402070843884E-4	0.0	5.271200637182733E-5	0.0
10-11	4.612300557534892E-5	1.7131402070843884E-4	0.0	7.9068009557741E-5	0.0
12-13	5.271200637182733E-5	1.7131402070843884E-4	0.0	1.0542401274365465E-4	6.589000796478416E-6
14-15	6.589000796478417E-5	3.096830374344856E-4	0.0	1.1201301354013307E-4	1.9767002389435247E-5
16-17	7.9068009557741E-5	3.16272038230964E-4	0.0	1.1860201433661149E-4	2.6356003185913664E-5
18-19	7.9068009557741E-5	3.2945003982392087E-4	0.0	1.251910151330899E-4	3.2945003982392084E-5
20-21	7.9068009557741E-5	3.4262804141687767E-4	0.0	1.9108102309787405E-4	4.612300557534892E-5
22-23	7.9068009557741E-5	4.216960509746187E-4	0.0	2.108480254873093E-4	5.930100716830575E-5
24-25	7.9068009557741E-5	4.74408057346446E-4	0.0	2.2402602708026614E-4	6.589000796478417E-5
26-27	7.9068009557741E-5	4.941750597358812E-4	0.0	2.503820302661798E-4	7.247900876126258E-5
28-29	7.9068009557741E-5	5.07353061328838E-4	0.0	5.139420621253165E-4	7.9068009557741E-5
30-31	7.9068009557741E-5	5.468870661077085E-4	0.0	0.0014693471776146868	8.56570103542194E-5
32-33	9.224601115069783E-5	5.666540684971438E-4	0.0	0.004039057488241269	9.224601115069782E-5
34-35	1.0542401274365465E-4	6.523110788513632E-4	0.0	0.007603706919136092	1.0542401274365465E-4
36-37	1.0542401274365465E-4	7.379680892055827E-4	0.0	0.012927619562690652	1.0542401274365465E-4
38-39	1.1201301354013307E-4	7.972690963738883E-4	0.0	0.02103867954315558	1.0542401274365465E-4
40-41	1.58136019115482E-4	8.763371059316294E-4	0.0	0.030184212648667625	1.0542401274365465E-4
42-43	1.7131402070843884E-4	8.895151075245863E-4	0.0	0.03797900059090159	1.0542401274365465E-4
44-45	2.2402602708026614E-4	9.356381130999352E-4	0.0	0.04734197072269742	1.1201301354013307E-4
46-47	3.0309403663800714E-4	9.883501194717624E-4	0.0	0.05829947904724103	1.3836901672604677E-4
48-49	3.294500398239208E-4	0.0010081171218611976	0.0	0.0681368572363833	1.9767002389435248E-4
50-51	3.294500398239208E-4	0.0011003631330118954	0.0	0.07831027446614597	1.9767002389435248E-4
52-53	3.294500398239208E-4	0.0011201301354013308	0.0	0.08802905064095165	1.9767002389435248E-4
54-55	3.294500398239208E-4	0.0011333081369942876	0.0	0.09797185284283758	1.9767002389435248E-4
56-57	3.294500398239208E-4	0.001172842141773158	0.0	0.106847236915694	2.042590246908309E-4
58-59	3.623950438063129E-4	0.001251910151330899	0.0	0.11500441990173428	2.108480254873093E-4
60-61	3.689840446027913E-4	0.0012914441561097695	0.0	0.12256200381529503	2.108480254873093E-4
62-63	3.689840446027913E-4	0.0013243891600921618	0.0	0.1295661116619516	2.3720402867322298E-4
64-65	3.755730453992697E-4	0.0013968681688534242	0.0	0.1368535465428567	2.3720402867322298E-4
66-67	3.8216204619574815E-4	0.0013968681688534242	0.0	0.14414098142376183	2.4379302946970138E-4
68-69	3.8216204619574815E-4	0.001410046170446381	0.0	0.152186151396262	2.503820302661798E-4
70-71	3.8216204619574815E-4	0.0014429911744287733	0.0	0.15943405227238824	2.6356003185913667E-4
72-73	3.8216204619574815E-4	0.0014627581768182085	0.0	0.16577926003939694	3.16272038230964E-4
74-75	3.9534004778870495E-4	0.0014693471776146868	0.0	0.1714392117235719	3.16272038230964E-4
76-77	3.9534004778870495E-4	0.0015418261863759494	0.0	0.17686195937907367	3.2286103902744244E-4
78-79	4.019290485851834E-4	0.0015813601911548198	0.0	0.18156650594775925	3.294500398239208E-4
80-81	4.085180493816618E-4	0.0015813601911548198	0.0	0.18417575026316468	3.6898404460279134E-4
82-83	4.1510705017814024E-4	0.0015945381927477767	0.0	0.18590206847184204	4.085180493816618E-4
84-85	4.216960509746186E-4	0.0016208941959336904	0.0	0.18737800465025323	4.085180493816618E-4
86-87	4.216960509746186E-4	0.0016736062023055178	0.0	0.18766792068529825	4.085180493816618E-4
88-89	4.216960509746186E-4	0.0017856192158456508	0.0	0.18770745469007713	4.085180493816618E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8250	0.0	45.211796	1
GTATCAA	14315	0.0	33.372005	1
TCAACGC	17215	0.0	26.973791	4
ATCAACG	17310	0.0	26.825758	3
CAACGCA	17715	0.0	26.212467	5
TATCAAC	18525	0.0	25.38247	2
AACGCAG	18400	0.0	25.37267	6
ACGCAGA	20940	0.0	22.235218	7
CGCAGAG	21280	0.0	21.89956	8
GTACATG	20180	0.0	18.83499	1
TACATGG	20335	0.0	18.527285	2
GCAGAGT	25820	0.0	18.097378	9
ACATGGG	20305	0.0	18.01982	3
AGAGTAC	23030	0.0	17.835104	10-11
GAGTACT	14510	0.0	17.309496	12-13
CAGAGTA	25375	0.0	16.482845	10-11
CATGGGG	14445	0.0	16.203138	4
AGTACTT	15895	0.0	15.748748	12-13
CTTATAC	5765	0.0	15.704661	1
GTACTTT	16685	0.0	15.054129	14-15
>>END_MODULE