FastQCFastQC Report
Fri 27 May 2016
SRR2139811_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139811_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8650918
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91100.044.9327241
GTATCAA158250.033.517881
TCAACGC183600.027.6108134
ATCAACG185650.027.3510573
CAACGCA189600.026.759155
AACGCAG197750.025.7622346
TATCAAC202050.025.3810232
ACGCAGA228850.022.1696957
CGCAGAG232600.021.8302848
GTACATG216250.018.870761
TACATGG216750.018.2666652
GCAGAGT283250.017.8822789
AGAGTAC251100.017.5189910-11
ACATGGG220150.017.3937823
GAGTACT157400.017.1681212-13
CAGAGTA276750.016.45539910-11
CATGGGG165200.015.79954054
GTACTTT180400.014.99149614-15
AGTACTT182050.014.80899512-13
GTGGTAT47650.013.8045841
Produced by FastQC (version 0.11.5)