##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139811_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8650918
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05182282388991	33.0	31.0	34.0	30.0	34.0
2	32.20943684820501	33.0	31.0	34.0	30.0	34.0
3	32.26249965610586	34.0	31.0	34.0	30.0	34.0
4	35.90937955948721	37.0	35.0	37.0	35.0	37.0
5	35.78667385357254	37.0	35.0	37.0	35.0	37.0
6	35.80058809943638	37.0	35.0	37.0	35.0	37.0
7	35.720412215212306	37.0	35.0	37.0	33.0	37.0
8	35.6996574236399	37.0	35.0	37.0	33.0	37.0
9	37.3367406788505	39.0	37.0	39.0	34.0	39.0
10-11	37.246380384139584	39.0	37.0	39.0	33.0	39.0
12-13	37.22564142903678	39.0	37.0	39.0	33.0	39.0
14-15	38.44615409601617	40.0	38.0	41.0	33.5	41.0
16-17	38.426285279781865	40.0	38.0	41.0	33.0	41.0
18-19	38.45651669568478	40.0	38.0	41.0	33.5	41.0
20-21	38.42895929636829	40.0	38.0	41.0	34.0	41.0
22-23	38.36547402287072	40.0	38.0	41.0	34.0	41.0
24-25	38.284068743451854	40.0	38.0	41.0	33.5	41.0
26-27	38.13945071042411	40.0	38.0	41.0	33.0	41.0
28-29	38.043751536743144	40.0	37.0	41.0	33.0	41.0
30-31	37.93439590305708	40.0	37.0	41.0	33.0	41.0
32-33	38.10480305640566	40.0	37.0	41.0	33.0	41.0
34-35	38.0957273085924	40.0	37.0	41.0	33.0	41.0
36-37	38.016132998744936	40.0	37.0	41.0	33.0	41.0
38-39	37.8911659711797	40.0	37.0	41.0	33.0	41.0
40-41	37.84869731166535	40.0	37.0	41.0	33.0	41.0
42-43	37.77186932118923	40.0	37.0	41.0	33.0	41.0
44-45	37.77486502515853	40.0	37.0	41.0	33.0	41.0
46-47	37.81281780831801	40.0	37.0	41.0	33.0	41.0
48-49	37.742669858101486	40.0	37.0	41.0	33.0	41.0
50-51	37.68088603962521	40.0	37.0	41.0	32.0	41.0
52-53	37.547089154834055	40.0	36.0	41.0	32.0	41.0
54-55	37.43000491457443	39.0	36.0	41.0	32.0	41.0
56-57	37.27060768603144	39.0	36.0	41.0	31.0	41.0
58-59	37.11570024049985	39.0	35.5	40.5	31.0	41.0
60-61	36.96614665233504	39.0	35.0	40.0	31.0	41.0
62-63	36.745548671613044	38.0	35.0	40.0	31.0	41.0
64-65	36.528807134944905	38.0	35.0	40.0	31.0	41.0
66-67	36.27725103237772	37.5	35.0	40.0	30.5	41.0
68-69	35.987165267805494	37.0	34.0	40.0	30.0	41.0
70-71	35.701632781292446	36.5	34.0	39.0	30.0	41.0
72-73	35.38337587256538	36.0	34.0	39.0	30.0	40.5
74-75	35.04031319937091	36.0	34.0	38.5	29.5	40.0
76-77	33.9510683509851	35.0	32.5	37.0	28.5	39.0
78-79	34.303527000560564	35.0	33.0	37.0	29.0	39.0
80-81	34.18604311382528	35.0	33.0	37.0	29.0	39.0
82-83	33.93393487569063	35.0	33.0	36.0	29.0	38.5
84-85	33.71596133514541	35.0	33.0	36.0	29.0	37.0
86-87	33.52413738545029	35.0	33.0	35.5	29.0	37.0
88-89	33.31727721106131	35.0	33.0	35.0	29.0	36.5
90-91	33.193592001075984	35.0	33.0	35.0	29.0	36.0
92-93	33.1287417326421	35.0	33.0	35.0	29.0	36.0
94-95	33.094260402487976	34.5	33.0	35.0	29.0	36.0
96-97	33.048992075807206	34.0	33.0	35.0	29.0	35.0
98-99	33.12401255111941	34.0	33.0	35.0	29.0	35.0
100-101	32.61521643023558	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	1.0
17	0.0
18	4.0
19	13.0
20	54.0
21	325.0
22	1452.0
23	4883.0
24	12886.0
25	27357.0
26	48714.0
27	77128.0
28	111961.0
29	149254.0
30	192088.0
31	241026.0
32	303025.0
33	385462.0
34	509591.0
35	697380.0
36	1046198.0
37	1723774.0
38	2079286.0
39	999108.0
40	39946.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.8996078797649	22.234102785392256	15.494413425257298	24.37187590958555
2	13.902380914673612	22.91449027192734	40.98379265305009	22.199336160348963
3	16.978082557249994	27.76698380449335	31.573215698033437	23.681717940223223
4	12.244503993680208	19.816602122456832	39.48814449518537	28.450749388677593
5	12.708200447628796	38.30677854072828	36.336039712779616	12.648981298863312
6	28.51897336213336	39.53440548159167	19.312759640075193	12.633861516199785
7	24.912107593668097	33.065196086704326	24.761464621442485	17.261231698185096
8	23.414775171837256	37.555320718564204	22.001688144541422	17.028215965057118
9	25.13785242213601	16.77719058254858	21.808737523578422	36.27621947173699
10-11	23.52063098968225	27.228214392969623	29.891336387652736	19.359818229695392
12-13	24.443522898004748	24.827106488993795	29.728395860901458	21.000974752099992
14-15	21.679704974662805	26.16312511573916	27.141373898122716	25.015796011475317
16-17	21.340082058343405	29.11308372128831	27.848298874177285	21.698535346191004
18-19	21.394362244964306	28.462452775717136	29.291918242928677	20.851266736389885
20-21	22.12843921520755	27.696590432040914	29.038315914906544	21.136654437844992
22-23	21.884931750112997	27.69878125346299	28.858495486705216	21.557791509718797
24-25	21.73385691886972	27.836999600014202	28.857610952695868	21.571532528420214
26-27	21.694471875338387	27.934736735915923	28.801719446585956	21.569071942159738
28-29	21.665850023023623	27.98843685841946	28.811170365598443	21.53454275295848
30-31	21.89614793278536	27.83080338600411	28.731162705867035	21.54188597534349
32-33	21.721540940227232	27.983649232120268	28.684225504921145	21.610584322731356
34-35	21.820150015130178	28.017782575199718	28.703060397927427	21.459007011742685
36-37	21.806986410080924	27.973305875921962	28.645257211729398	21.57445050226772
38-39	21.678236903549767	28.046590457268188	28.692136525648998	21.583036113533048
40-41	21.875674768165116	27.92844627585046	28.60511092594125	21.590768030043176
42-43	21.800180735382067	28.037882519604633	28.41156011309145	21.750376631921846
44-45	21.89796248147151	28.085415492865295	28.25311796834622	21.76350405731698
46-47	21.957604336952375	28.071776016200268	28.029639812834272	21.940979834013085
48-49	21.8731928502991	28.196184387908158	28.041980462350885	21.888642299441855
50-51	21.893344636965413	28.25826851258525	27.960723999382868	21.88766285106647
52-53	22.03460183888869	28.141290957657745	27.906374430565776	21.91773277288779
54-55	21.975108981068043	28.093138575875532	27.92373475467395	22.00801768838247
56-57	22.030853751433554	28.15485982618816	27.874490987290347	21.939795435087937
58-59	21.967117915741106	28.207284737139194	27.889399721366466	21.936197625753234
60-61	22.0635109295353	28.185446221766508	27.89040852520639	21.8606343234918
62-63	22.038179919131515	28.259307270957905	27.861313927269897	21.84119888264068
64-65	21.98193171540898	28.192353263741065	27.844702944247135	21.981012076602823
66-67	22.041373973795377	28.202784402273707	27.75369405145238	22.002147572478535
68-69	22.00172994302626	28.173656366008874	27.803802685315887	22.020811005648984
70-71	22.02676461833349	27.99862086695939	27.868377146117464	22.10623736858966
72-73	22.033735874328737	28.032199427475536	27.8032262847131	22.13083841348262
74-75	21.92377833363234	28.12073798432138	27.812294578824844	22.143189103221435
76-77	22.035146919666847	28.03385431153301	27.765955279457515	22.16504348934263
78-79	22.007771579822293	28.040346042822378	27.72742594691653	22.224456430438796
80-81	22.002509988205844	28.03684592601326	27.778289225655868	22.182354860125027
82-83	22.013406956813313	27.999622814481818	27.85600159550679	22.13096863319808
84-85	22.072396467914203	28.005383276564267	27.806890804847516	22.11532945067401
86-87	22.003363164220673	27.962492052908367	27.868436934779822	22.16570784809114
88-89	22.018143769756602	27.942800008824488	27.84007355837869	22.198982663040223
90-91	22.070162280993607	27.92265097899414	27.877760879263008	22.129425860749244
92-93	22.10112523291655	27.8462628845697	27.903918624997846	22.1486932575159
94-95	21.95186413298755	27.840409352134117	27.97420325762545	22.23352325725288
96-97	21.89623104147498	27.907815035320134	28.050287451378125	22.145666471826765
98-99	22.11026279910445	28.499984245831953	28.11279663896841	21.276956316095188
100-101	22.677474511430546	31.147978348978082	25.322643885408258	20.851903254183114
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1490.0
1	1375.5
2	1960.5
3	2801.5
4	3721.0
5	5798.5
6	7507.5
7	8645.5
8	9335.5
9	9246.0
10	8721.0
11	8230.5
12	9121.0
13	11988.0
14	18745.0
15	28286.5
16	36183.5
17	40619.0
18	42116.5
19	41445.5
20	39556.0
21	37989.0
22	38447.5
23	42756.5
24	51660.5
25	65245.0
26	82646.0
27	101916.5
28	123932.0
29	147771.5
30	170179.5
31	192299.0
32	214836.0
33	236155.0
34	256599.5
35	276229.0
36	295965.5
37	314113.5
38	328976.5
39	341357.0
40	352480.0
41	361825.0
42	366986.5
43	370408.5
44	372800.5
45	373985.5
46	373420.0
47	370322.5
48	365141.5
49	356852.5
50	345562.5
51	330184.0
52	311611.5
53	291455.0
54	269578.5
55	247114.5
56	225552.0
57	202551.5
58	177388.5
59	151665.5
60	125647.5
61	101606.5
62	80583.5
63	62308.0
64	46765.5
65	34960.0
66	26101.0
67	19527.5
68	14712.0
69	10569.0
70	7428.5
71	5355.5
72	3762.5
73	2556.0
74	1703.0
75	1122.0
76	751.0
77	486.5
78	324.0
79	215.0
80	149.0
81	117.5
82	90.0
83	69.0
84	49.0
85	31.0
86	23.0
87	19.5
88	15.0
89	9.5
90	6.5
91	5.5
92	5.5
93	3.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003872421400827057
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	5.779733434070234E-6
14-15	0.0
16-17	0.0
18-19	5.259557425003913E-4
20-21	0.0012600319204233198
22-23	0.0028266725982473936
24-25	2.8909054946226558E-5
26-27	0.007617469976207697
28-29	1.678230251801667E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.03449535220363582
38-39	0.014633696631613232
40-41	0.023697472608378494
42-43	0.0013183441597353724
44-45	0.005692606278418416
46-47	0.004507618241798917
48-49	0.0027386628784509086
50-51	0.002210228299725688
52-53	1.8904698327961353E-4
54-55	0.0024555285334324026
56-57	0.014417844959635821
58-59	0.009857388860214884
60-61	0.0033178731395108498
62-63	0.0011356498553739948
64-65	0.0029937710096774655
66-67	2.2407276145030482E-4
68-69	5.155159651513957E-4
70-71	1.8095172674229197E-4
72-73	0.0
74-75	0.0
76-77	0.005699808222041989
78-79	2.0495788737608008E-4
80-81	7.424242840449978E-6
82-83	1.506329672600752E-4
84-85	0.0017875691069251267
86-87	0.005379161098899055
88-89	0.07632076893292794
90-91	0.0028185639912915983
92-93	0.0013047804955540012
94-95	0.0
96-97	0.017295585823825236
98-99	2.6701972608226434E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	208.0
20-21	671.0
22-23	1418.0
24-25	3081.0
26-27	4052.0
28-29	6995.0
30-31	19803.0
32-33	26052.0
34-35	36877.0
36-37	58413.0
38-39	65024.0
40-41	66871.0
42-43	75089.0
44-45	88470.0
46-47	89065.0
48-49	81677.0
50-51	74624.0
52-53	73201.0
54-55	79970.0
56-57	83613.0
58-59	84332.0
60-61	83084.0
62-63	79151.0
64-65	83212.0
66-67	89277.0
68-69	89897.0
70-71	89570.0
72-73	86314.0
74-75	87702.0
76-77	89029.0
78-79	95251.0
80-81	96691.0
82-83	93909.0
84-85	93414.0
86-87	98406.0
88-89	104651.0
90-91	112676.0
92-93	115062.0
94-95	140650.0
96-97	183111.0
98-99	696993.0
100-101	5023362.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.93816455830601
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.49622481859221	80.46010074561124
2	6.189070575753228	10.885110135071143
3	1.3355442439919483	3.52335928509187
4	0.4596738308394	1.6169149191800833
5	0.20462774040085666	0.8997293954282429
6	0.10979028679342505	0.5792853784166293
7	0.061565696709520244	0.37897820578719854
8	0.03556504253220344	0.2502019650176047
9	0.02461499034211232	0.19481373641752078
>10	0.0816748339439353	1.0957814093422067
>50	0.0014430156647712601	0.0826041330800541
>100	2.049244363133113E-4	0.033120691556210745
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1559466868140468E-5	0.0	0.0	0.0
3	0.0	2.3118933736280937E-5	0.0	0.0	0.0
4	0.0	5.7797334340702336E-5	0.0	0.0	0.0
5	1.1559466868140468E-5	5.7797334340702336E-5	0.0	0.0	0.0
6	2.3118933736280937E-5	5.7797334340702336E-5	0.0	0.0	0.0
7	2.3118933736280937E-5	5.7797334340702336E-5	0.0	0.0	0.0
8	2.3118933736280937E-5	5.7797334340702336E-5	0.0	0.0	0.0
9	2.3118933736280937E-5	1.1559466868140467E-4	0.0	0.0	1.1559466868140468E-5
10-11	4.623786747256187E-5	1.1559466868140467E-4	0.0	1.1559466868140468E-5	1.1559466868140468E-5
12-13	4.623786747256187E-5	1.1559466868140467E-4	0.0	1.1559466868140468E-5	1.1559466868140468E-5
14-15	4.623786747256187E-5	1.9073120332431772E-4	0.0	1.73392003022107E-5	2.3118933736280937E-5
16-17	5.7797334340702336E-5	2.1385013706059865E-4	0.0	2.3118933736280937E-5	3.46784006044214E-5
18-19	5.7797334340702336E-5	2.254096039287391E-4	0.0	2.889866717035117E-5	3.46784006044214E-5
20-21	5.7797334340702336E-5	2.3118933736280934E-4	0.0	3.46784006044214E-5	3.46784006044214E-5
22-23	6.357706777477257E-5	2.4274880423094984E-4	0.0	3.46784006044214E-5	3.46784006044214E-5
24-25	7.513653464291304E-5	2.8320693826944146E-4	0.0	4.0458134038491635E-5	3.46784006044214E-5
26-27	8.091626807698327E-5	3.0054613857165215E-4	0.0	8.66960015110535E-5	4.0458134038491635E-5
28-29	8.091626807698327E-5	3.0054613857165215E-4	0.0	3.063258720057224E-4	5.779733434070234E-5
30-31	9.247573494512375E-5	3.236650723079331E-4	0.0	0.0011906250874184681	6.93568012088428E-5
32-33	1.0403520181326421E-4	3.236650723079331E-4	0.0	0.003230870989645261	6.93568012088428E-5
34-35	1.0403520181326421E-4	3.3522453917607354E-4	0.0	0.006039821438603394	9.247573494512375E-5
36-37	1.0403520181326421E-4	3.6412320634642475E-4	0.0	0.010062515908716277	9.247573494512375E-5
38-39	1.0403520181326421E-4	4.045813403849164E-4	0.0	0.016408663219325395	9.247573494512375E-5
40-41	1.2715413554954515E-4	4.277002741211973E-4	0.0	0.02346571774232515	9.247573494512375E-5
42-43	1.2715413554954515E-4	4.565989412915485E-4	0.0	0.02962691358304402	9.247573494512375E-5
44-45	1.2715413554954515E-4	4.970570753300401E-4	0.0	0.036915157443406586	1.1559466868140469E-4
46-47	1.2715413554954515E-4	5.317354759344615E-4	0.0	0.04508770051918189	1.3293386898361538E-4
48-49	1.2715413554954515E-4	5.317354759344615E-4	0.0	0.05312730972597359	1.387136024176856E-4
50-51	1.3293386898361538E-4	5.43294942802602E-4	0.0	0.060929949861968404	1.5027306928582608E-4
52-53	1.387136024176856E-4	5.43294942802602E-4	0.0	0.06820663425546283	1.560528027198963E-4
54-55	1.387136024176856E-4	5.43294942802602E-4	0.0	0.07642541519871071	1.6183253615396654E-4
56-57	1.387136024176856E-4	5.664138765388829E-4	0.0	0.08463263667509044	1.8495146989024747E-4
58-59	1.387136024176856E-4	5.664138765388829E-4	0.0	0.09233124160927199	2.1385013706059865E-4
60-61	1.6183253615396654E-4	5.664138765388829E-4	0.0	0.10016278041243715	2.1962987049466888E-4
62-63	1.6183253615396654E-4	5.721936099729531E-4	0.0	0.10739322693845901	2.254096039287391E-4
64-65	1.6183253615396654E-4	6.357706777477257E-4	0.0	0.11494733853678882	2.3118933736280934E-4
66-67	1.6183253615396654E-4	6.357706777477257E-4	0.0	0.12305052481135528	2.3118933736280934E-4
68-69	1.6183253615396654E-4	6.646693449180768E-4	0.0	0.13104967588410849	2.3118933736280934E-4
70-71	1.7339200302210703E-4	6.820085452202877E-4	0.0	0.13890433362100993	2.3118933736280934E-4
72-73	1.849514698902475E-4	7.051274789565686E-4	0.0	0.14536029586686638	2.3118933736280934E-4
74-75	1.849514698902475E-4	7.513653464291304E-4	0.0	0.15072388849368357	2.3118933736280934E-4
76-77	1.849514698902475E-4	7.860437470335518E-4	0.0	0.15581005391566538	2.369690707968796E-4
78-79	1.849514698902475E-4	7.976032139016923E-4	0.0	0.16065347053341622	2.4852853766502007E-4
80-81	1.849514698902475E-4	7.976032139016923E-4	0.0	0.16329480871278634	2.6008800453316053E-4
82-83	1.849514698902475E-4	8.091626807698327E-4	0.0	0.16517322207885915	2.8320693826944146E-4
84-85	1.849514698902475E-4	8.091626807698327E-4	0.0	0.1667395298394922	2.889866717035117E-4
86-87	1.849514698902475E-4	8.207221476379732E-4	0.0	0.16704007597806383	2.889866717035117E-4
88-89	1.9651093675838796E-4	8.554005482423946E-4	0.0	0.16709209357897045	2.889866717035117E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9110	0.0	44.932724	1
GTATCAA	15825	0.0	33.51788	1
TCAACGC	18360	0.0	27.610813	4
ATCAACG	18565	0.0	27.351057	3
CAACGCA	18960	0.0	26.75915	5
AACGCAG	19775	0.0	25.762234	6
TATCAAC	20205	0.0	25.381023	2
ACGCAGA	22885	0.0	22.169695	7
CGCAGAG	23260	0.0	21.830284	8
GTACATG	21625	0.0	18.87076	1
TACATGG	21675	0.0	18.266665	2
GCAGAGT	28325	0.0	17.882278	9
AGAGTAC	25110	0.0	17.51899	10-11
ACATGGG	22015	0.0	17.393782	3
GAGTACT	15740	0.0	17.16812	12-13
CAGAGTA	27675	0.0	16.455399	10-11
CATGGGG	16520	0.0	15.7995405	4
GTACTTT	18040	0.0	14.991496	14-15
AGTACTT	18205	0.0	14.808995	12-13
GTGGTAT	4765	0.0	13.804584	1
>>END_MODULE