FastQCFastQC Report
Fri 27 May 2016
SRR2139810_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139810_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7338689
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70750.044.4239731
GTATCAA124200.033.8767431
TCAACGC143350.028.2059084
ATCAACG145900.027.7706073
CAACGCA148350.027.3403385
AACGCAG152750.026.718066
TATCAAC161100.025.6471562
ACGCAGA177150.023.0142617
CGCAGAG177450.022.927938
TACATGG172300.019.4868162
GTACATG173700.019.475091
ACATGGG175450.018.4651033
GCAGAGT218150.018.418859
AGAGTAC195050.017.76364310-11
GAGTACT128100.016.96428512-13
CAGAGTA215450.016.48201610-11
CATGGGG128100.015.8968334
AGTACTT146750.015.29574812-13
GTACTTT147550.014.60063614-15
GTATTAG25700.014.2432591
Produced by FastQC (version 0.11.5)