##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139810_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7338689
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05645913050682	33.0	31.0	34.0	30.0	34.0
2	32.20618873479991	33.0	31.0	34.0	30.0	34.0
3	32.26113042806419	34.0	31.0	34.0	30.0	34.0
4	35.90842001888893	37.0	35.0	37.0	35.0	37.0
5	35.78678085418254	37.0	35.0	37.0	35.0	37.0
6	35.80141562069193	37.0	35.0	37.0	35.0	37.0
7	35.721917906590676	37.0	35.0	37.0	33.0	37.0
8	35.70085310877733	37.0	35.0	37.0	33.0	37.0
9	37.33932927257171	39.0	37.0	39.0	34.0	39.0
10-11	37.248189833361245	39.0	37.0	39.0	33.0	39.0
12-13	37.22880040290575	39.0	37.0	39.0	33.0	39.0
14-15	38.45399484839867	40.0	38.0	41.0	34.0	41.0
16-17	38.43630585789914	40.0	38.0	41.0	33.0	41.0
18-19	38.466578090446404	40.0	38.0	41.0	33.5	41.0
20-21	38.434910336810205	40.0	38.0	41.0	34.0	41.0
22-23	38.37071385676116	40.0	38.0	41.0	34.0	41.0
24-25	38.2918675625583	40.0	38.0	41.0	33.5	41.0
26-27	38.15276703923464	40.0	38.0	41.0	33.0	41.0
28-29	38.056656061737314	40.0	37.0	41.0	33.0	41.0
30-31	37.95235688032759	40.0	37.0	41.0	33.0	41.0
32-33	38.11742372426821	40.0	37.5	41.0	33.0	41.0
34-35	38.105123627877774	40.0	37.5	41.0	33.0	41.0
36-37	38.02679741277747	40.0	37.0	41.0	33.0	41.0
38-39	37.90307745702594	40.0	37.0	41.0	33.0	41.0
40-41	37.85466195310043	40.0	37.0	41.0	33.0	41.0
42-43	37.77820914037811	40.0	37.0	41.0	33.0	41.0
44-45	37.783301843224166	40.0	37.0	41.0	33.0	41.0
46-47	37.82224103223512	40.0	37.0	41.0	33.0	41.0
48-49	37.75441810639131	40.0	37.0	41.0	33.0	41.0
50-51	37.69240042597598	40.0	37.0	41.0	32.0	41.0
52-53	37.56100289002291	40.0	36.5	41.0	32.0	41.0
54-55	37.44483831024513	39.0	36.0	41.0	32.0	41.0
56-57	37.28755985001651	39.0	36.0	41.0	31.0	41.0
58-59	37.13321808078261	39.0	35.5	40.5	31.0	41.0
60-61	36.98519268313382	39.0	35.0	40.0	31.0	41.0
62-63	36.76870765549588	38.5	35.0	40.0	31.0	41.0
64-65	36.55346794228571	38.0	35.0	40.0	31.0	41.0
66-67	36.30340142760916	38.0	35.0	40.0	30.5	41.0
68-69	36.01438713279168	37.0	34.0	40.0	30.0	41.0
70-71	35.73336864205194	37.0	34.0	39.0	30.0	41.0
72-73	35.41287580030556	36.0	34.0	39.0	30.0	40.5
74-75	35.06925780040339	36.0	34.0	38.5	29.5	40.0
76-77	33.97897034221026	35.0	32.5	37.0	28.5	39.0
78-79	34.333509810873856	35.0	33.0	37.0	29.0	39.0
80-81	34.2186150200106	35.0	33.0	37.0	29.0	39.0
82-83	33.96354116091219	35.0	33.0	36.0	29.0	38.5
84-85	33.73990343753867	35.0	33.0	36.0	29.0	37.0
86-87	33.54620340398256	35.0	33.0	35.5	29.0	37.0
88-89	33.335080986370926	35.0	33.0	35.0	29.0	36.5
90-91	33.20828003388337	35.0	33.0	35.0	29.0	36.0
92-93	33.13978272898696	35.0	33.0	35.0	29.0	36.0
94-95	33.101429466692025	34.5	33.0	35.0	29.0	36.0
96-97	33.0526965487901	34.0	33.0	35.0	29.0	35.0
98-99	33.12626849378492	34.0	33.0	35.0	29.0	35.0
100-101	32.612911286083175	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	7.0
20	41.0
21	267.0
22	1248.0
23	4167.0
24	10602.0
25	22428.0
26	41032.0
27	65633.0
28	94193.0
29	126734.0
30	162699.0
31	204515.0
32	256415.0
33	326321.0
34	431198.0
35	588758.0
36	884346.0
37	1458175.0
38	1775244.0
39	850956.0
40	33707.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.748772294343034	22.469340232294897	15.363588782683118	24.41829869067895
2	13.723430392920106	23.049029810668873	41.372419350069286	21.855120446341736
3	16.92443977391602	28.130596622911803	31.491237740146776	23.453725863025397
4	12.094885067346498	19.834550830536628	39.86029384812465	28.21027025399223
5	12.533437511795361	38.486642505221305	36.5047353825731	12.475184600410238
6	28.257622035761425	39.91786543890877	19.414135140486263	12.410377384843532
7	24.672921825683034	33.07528088463757	25.093214878025215	17.15858241165418
8	23.064446524440537	38.13063886478906	22.125668494740683	16.679246116029717
9	24.93657927185632	16.98078498761836	21.947939202764964	36.134696537760355
10-11	23.399806150662606	27.42461221616013	30.084835315953573	19.09074631722369
12-13	24.288329972833022	24.924506270806678	29.924588710599398	20.8625750457609
14-15	21.480280742241565	26.272376442168348	27.248143367296258	24.99919944829383
16-17	21.253366916079973	29.27775655842617	27.907246103493417	21.56163042200044
18-19	21.34040221032539	28.637774968904356	29.28058410759039	20.741238713179865
20-21	22.06505869569187	27.927112577500306	28.9883445315696	21.019484195238224
22-23	21.741591546900246	27.909889261035193	28.86949248911363	21.479026702950932
24-25	21.63266952994694	28.014132289219297	28.875958478255775	21.47723970257799
26-27	21.640326864743287	28.1116716504253	28.790646543982607	21.457354940848806
28-29	21.580996929894425	28.155129525301852	28.829829651946692	21.434043892857034
30-31	21.828684278387126	28.03086612994517	28.70874745680113	21.431702134866573
32-33	21.67359484138303	28.169924856188093	28.669884231066384	21.48659607136249
34-35	21.752567544566894	28.15316059118184	28.695159443070622	21.39911242118064
36-37	21.700415606309797	28.14823625170726	28.67253726130889	21.478810880674054
38-39	21.573107049608357	28.19325205018345	28.750339636731777	21.483301263476424
40-41	21.789639077507303	28.071260357140133	28.632631120696043	21.50646944465652
42-43	21.688274581738728	28.175429917148076	28.453824178592026	21.682471322521174
44-45	21.79103891393919	28.234786166978093	28.332266152807208	21.64190876627551
46-47	21.850906008143447	28.212129647549588	28.12357921744339	21.813385126863572
48-49	21.752223995716694	28.305359094863686	28.14478590325852	21.797631006161104
50-51	21.76876684715677	28.35526723941072	28.094219266510784	21.781746646921725
52-53	21.931646951860532	28.23996310568767	28.035784878097658	21.79260506435414
54-55	21.861267342142348	28.19249637977994	28.075641128089696	21.870595149988013
56-57	21.88424288970179	28.31132034970581	28.020308789145187	21.784127971447216
58-59	21.877174820015355	28.357392159618243	27.980842101747587	21.78459091861881
60-61	21.908163947159576	28.311480396789506	28.01210866662777	21.768246989423147
62-63	21.890789997588563	28.368260208255837	27.96371381054838	21.77723598360722
64-65	21.843221035953597	28.356451221683194	27.941295679426354	21.85903206293686
66-67	21.91953012485316	28.371034181007204	27.83440759189226	21.875028102247377
68-69	21.87653732439422	28.352543890624037	27.89781999398458	21.87309879099716
70-71	21.89225597843173	28.135975102851695	27.949520365055147	22.022248553661427
72-73	21.957944887565247	28.201492793456424	27.878888088015145	21.961674230963187
74-75	21.791717237683265	28.280072246178023	27.899989154557243	22.02822136158147
76-77	21.8948945063198	28.28048246690885	27.85706926699516	21.967553759776184
78-79	21.849698297816143	28.264206569174448	27.84116191608724	22.044933216922164
80-81	21.895330404488163	28.276372188952514	27.83478057437715	21.99351683218217
82-83	21.900207656857056	28.285416187201847	27.874907755734984	21.939468400206117
84-85	21.932512313117254	28.25816356038221	27.875473455318428	21.93385067118211
86-87	21.813851672489573	28.251274890009576	27.95493003011312	21.97994340738773
88-89	21.85254712730977	28.25410818239685	27.929822736210035	21.96352195408335
90-91	21.89590929909073	28.27673891213006	27.944696197483232	21.882655591295972
92-93	21.903272293882047	28.2410144277225	27.917199070282027	21.93851420811343
94-95	21.742515825375648	28.312277348296846	27.9763473875598	21.96885943876771
96-97	21.700850594884848	28.332452007756192	28.042060849944306	21.924636547414657
98-99	21.912597636641618	28.931175042776513	28.125624880716355	21.03060243986552
100-101	22.473773488598045	31.624838217511652	25.323608548598887	20.577779745291416
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1539.0
1	1386.5
2	1834.5
3	2551.0
4	3268.0
5	4973.0
6	6429.0
7	7401.5
8	7945.0
9	7749.0
10	7237.0
11	6717.0
12	7240.5
13	9331.5
14	14418.0
15	21748.0
16	27989.5
17	31627.0
18	32772.5
19	32269.5
20	31291.5
21	30881.5
22	32241.0
23	36894.5
24	45406.0
25	57950.5
26	74014.0
27	91762.5
28	111315.0
29	132394.0
30	151771.5
31	170441.5
32	189324.5
33	207262.5
34	225025.5
35	241392.5
36	257784.0
37	271705.0
38	281810.0
39	290670.0
40	299722.5
41	306941.5
42	309636.0
43	312159.0
44	314900.0
45	314798.5
46	311932.5
47	307921.0
48	301854.0
49	293301.5
50	283151.0
51	271101.0
52	256149.0
53	239465.5
54	222477.0
55	203757.5
56	184599.0
57	166087.5
58	146240.0
59	124966.5
60	103785.5
61	84275.0
62	66792.0
63	51293.5
64	38127.5
65	28197.0
66	21103.0
67	15922.5
68	11959.0
69	8410.0
70	5846.0
71	4197.0
72	2952.0
73	1985.5
74	1290.0
75	853.0
76	555.5
77	366.5
78	249.5
79	175.0
80	124.0
81	89.0
82	69.5
83	49.5
84	36.5
85	27.0
86	21.5
87	19.0
88	13.0
89	8.0
90	5.5
91	3.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003760889717495864
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.586828928164146E-4
20-21	0.0011855372630032658
22-23	0.0024939358518814704
24-25	3.407800359604725E-5
26-27	0.0077999386641186864
28-29	2.182938872868164E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.03431794430547991
38-39	0.015493091060555905
40-41	0.02457754674246411
42-43	0.001290379991522979
44-45	0.005549647394614865
46-47	0.004910040933063058
48-49	0.0030502819368331637
50-51	0.0022585808104814576
52-53	2.4420517763817625E-4
54-55	0.0027855687821530624
56-57	0.014672917656491383
58-59	0.01032331257869715
60-61	0.0037147443211616882
62-63	0.0012072611069774317
64-65	0.0035414187017757145
66-67	2.307860652965402E-4
68-69	4.831639508155204E-4
70-71	2.9343610335112996E-4
72-73	0.0
74-75	0.0
76-77	0.00536654064169966
78-79	3.252335626315538E-4
80-81	0.0
82-83	1.232122236382827E-4
84-85	0.0016416922742407687
86-87	0.0051932072125867425
88-89	0.0758464538637473
90-91	0.0030799921562866418
92-93	0.0014814489824582196
94-95	0.0
96-97	0.01641059039354777
98-99	1.034999008988449E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	145.0
20-21	514.0
22-23	1171.0
24-25	2668.0
26-27	3381.0
28-29	5788.0
30-31	15892.0
32-33	20371.0
34-35	28642.0
36-37	46765.0
38-39	53525.0
40-41	54511.0
42-43	60779.0
44-45	70975.0
46-47	71970.0
48-49	67098.0
50-51	63043.0
52-53	60243.0
54-55	66033.0
56-57	69730.0
58-59	70276.0
60-61	69233.0
62-63	65852.0
64-65	68823.0
66-67	73649.0
68-69	74567.0
70-71	75086.0
72-73	71271.0
74-75	72691.0
76-77	72434.0
78-79	79356.0
80-81	81192.0
82-83	77980.0
84-85	76754.0
86-87	80612.0
88-89	86063.0
90-91	91193.0
92-93	95666.0
94-95	118031.0
96-97	156208.0
98-99	594878.0
100-101	4323630.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.63194684205877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.02191306425368	81.56081307015485
2	5.814136134229122	10.306364095629771
3	1.2376968888920181	3.290984545885766
4	0.4331385530281662	1.5355965282895472
5	0.20097164405092866	0.8906254036141533
6	0.10016660438282594	0.5326776693004895
7	0.05864262753254739	0.3638327172300374
8	0.03348740834858921	0.23744433573043788
9	0.022506132930234454	0.17952861397435635
>10	0.07633476642424687	1.014816548227835
>50	7.776651521139952E-4	0.04362190181725178
>100	2.28510775562824E-4	0.0436945701454738
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.3626412019912549E-5	0.0	0.0	0.0
4	0.0	4.087923605973765E-5	0.0	0.0	0.0
5	0.0	6.813206009956274E-5	0.0	0.0	0.0
6	0.0	6.813206009956274E-5	0.0	0.0	1.3626412019912549E-5
7	0.0	6.813206009956274E-5	0.0	0.0	1.3626412019912549E-5
8	0.0	6.813206009956274E-5	0.0	0.0	2.7252824039825097E-5
9	0.0	1.2263770817921293E-4	0.0	2.7252824039825097E-5	2.7252824039825097E-5
10-11	0.0	1.2263770817921293E-4	0.0	2.7252824039825097E-5	2.7252824039825097E-5
12-13	0.0	1.2263770817921293E-4	0.0	2.7252824039825097E-5	2.7252824039825097E-5
14-15	1.3626412019912549E-5	1.9076976827877567E-4	0.0	2.7252824039825097E-5	2.7252824039825097E-5
16-17	1.3626412019912549E-5	2.0439618029868822E-4	0.0	2.7252824039825097E-5	2.7252824039825097E-5
18-19	1.3626412019912549E-5	2.3164900433851333E-4	0.0	5.4505648079650194E-5	2.7252824039825097E-5
20-21	2.7252824039825097E-5	2.4527541635842586E-4	0.0	1.0219809014934411E-4	2.7252824039825097E-5
22-23	2.7252824039825097E-5	2.7252824039825096E-4	0.0	1.0901129615930039E-4	3.406603004978137E-5
24-25	3.406603004978137E-5	3.134074764579886E-4	0.0	1.4307732620908175E-4	4.087923605973765E-5
26-27	4.087923605973765E-5	3.406603004978137E-4	0.0	2.1802259231860078E-4	4.087923605973765E-5
28-29	4.087923605973765E-5	3.4747350650776997E-4	0.0	7.97145103164884E-4	5.4505648079650194E-5
30-31	4.087923605973765E-5	4.22418772617289E-4	0.0	0.0028206672881218977	6.813206009956274E-5
32-33	4.087923605973765E-5	4.3604518463720155E-4	0.0	0.008087275533818097	8.17584721194753E-5
34-35	4.087923605973765E-5	5.109904507467206E-4	0.0	0.014491689183176995	9.538488413938784E-5
36-37	4.087923605973765E-5	5.927489228661959E-4	0.0	0.02326028531799072	9.538488413938784E-5
38-39	4.087923605973765E-5	6.336281589259334E-4	0.0	0.03585790323039987	9.538488413938784E-5
40-41	4.087923605973765E-5	8.107715151847966E-4	0.0	0.04757661756752467	9.538488413938784E-5
42-43	4.087923605973765E-5	8.243979272047092E-4	0.0	0.057680602080289814	9.538488413938784E-5
44-45	4.087923605973765E-5	9.129696053341407E-4	0.0	0.06852041284213026	9.538488413938784E-5
46-47	6.813206009956274E-5	9.606620474038347E-4	0.0	0.07932615757392089	9.538488413938784E-5
48-49	6.813206009956274E-5	0.0010015412834635724	0.0	0.08882376675179995	9.538488413938784E-5
50-51	6.813206009956274E-5	0.0011105525796228726	0.0	0.09821236463351969	9.538488413938784E-5
52-53	7.494526610951902E-5	0.0011309921976527415	0.0	0.10629282696132783	1.1582450216925667E-4
54-55	8.17584721194753E-5	0.0011786846397224353	0.0	0.1144618609672654	1.3626412019912548E-4
56-57	8.17584721194753E-5	0.0011854978457323917	0.0	0.12272627985734237	1.3626412019912548E-4
58-59	8.17584721194753E-5	0.0011854978457323917	0.0	0.1304933347086925	1.3626412019912548E-4
60-61	8.857167812943156E-5	0.001199124257752304	0.0	0.13804236696772407	1.3626412019912548E-4
62-63	9.538488413938784E-5	0.0012263770817921293	0.0	0.14491689183176995	1.4989053221903804E-4
64-65	9.538488413938784E-5	0.0013353883779514298	0.0	0.15183229593187558	1.4989053221903804E-4
66-67	9.538488413938784E-5	0.0013558279959812985	0.0	0.15853649064567255	1.5670373822899433E-4
68-69	1.0901129615930039E-4	0.0013762676140111675	0.0	0.1658675003123855	1.635169442389506E-4
70-71	1.0901129615930039E-4	0.0013762676140111675	0.0	0.17306224585889932	1.635169442389506E-4
72-73	1.0901129615930039E-4	0.0013762676140111675	0.0	0.17931676897603918	2.1802259231860078E-4
74-75	1.0901129615930039E-4	0.0014035204380509923	0.0	0.18533282988283056	2.1802259231860078E-4
76-77	1.0901129615930039E-4	0.001417146850070905	0.0	0.1898636118794515	2.1802259231860078E-4
78-79	1.0901129615930039E-4	0.001417146850070905	0.0	0.19444889952415206	2.1802259231860078E-4
80-81	1.0901129615930039E-4	0.0014307732620908176	0.0	0.1968471480396567	2.1802259231860078E-4
82-83	1.0901129615930039E-4	0.0014580260861306428	0.0	0.19850275710007603	2.1802259231860078E-4
84-85	1.0901129615930039E-4	0.0014716524981505553	0.0	0.19994715677418679	2.2483579832855707E-4
86-87	1.0901129615930039E-4	0.0014716524981505553	0.0	0.20021968501458504	2.3164900433851333E-4
88-89	1.0901129615930039E-4	0.0014989053221903803	0.0	0.20028100386867465	2.3164900433851333E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7075	0.0	44.423973	1
GTATCAA	12420	0.0	33.876743	1
TCAACGC	14335	0.0	28.205908	4
ATCAACG	14590	0.0	27.770607	3
CAACGCA	14835	0.0	27.340338	5
AACGCAG	15275	0.0	26.71806	6
TATCAAC	16110	0.0	25.647156	2
ACGCAGA	17715	0.0	23.014261	7
CGCAGAG	17745	0.0	22.92793	8
TACATGG	17230	0.0	19.486816	2
GTACATG	17370	0.0	19.47509	1
ACATGGG	17545	0.0	18.465103	3
GCAGAGT	21815	0.0	18.41885	9
AGAGTAC	19505	0.0	17.763643	10-11
GAGTACT	12810	0.0	16.964285	12-13
CAGAGTA	21545	0.0	16.482016	10-11
CATGGGG	12810	0.0	15.896833	4
AGTACTT	14675	0.0	15.295748	12-13
GTACTTT	14755	0.0	14.600636	14-15
GTATTAG	2570	0.0	14.243259	1
>>END_MODULE