FastQCFastQC Report
Fri 27 May 2016
SRR2139809_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139809_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15296749
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA168650.046.4726871
GTATCAA293600.033.8649441
TCAACGC364400.026.5704634
ATCAACG368550.026.2712693
CAACGCA373850.025.8763945
AACGCAG386250.025.1108076
TATCAAC392100.024.919192
ACGCAGA452450.021.5664837
CGCAGAG455350.021.4107158
GTACATG392850.019.7798731
TACATGG399900.019.1060892
AGAGTAC479800.018.35323310-11
ACATGGG405000.018.1708663
GCAGAGT540500.017.9057969
GAGTACT315650.017.11731712-13
CATGGGG299650.016.6667544
CAGAGTA525450.016.41957910-11
AGTACTT354300.015.91872312-13
GTGGTAT85200.015.5532911
GTACTTT367400.014.68998314-15
Produced by FastQC (version 0.11.5)