##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139809_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15296749
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01254658751346	33.0	31.0	34.0	30.0	34.0
2	32.172553396803465	33.0	31.0	34.0	30.0	34.0
3	32.223816380853215	34.0	31.0	34.0	30.0	34.0
4	35.875042533547486	37.0	35.0	37.0	35.0	37.0
5	35.748582394860506	37.0	35.0	37.0	35.0	37.0
6	35.762752987579255	37.0	35.0	37.0	33.0	37.0
7	35.681932350462176	37.0	35.0	37.0	33.0	37.0
8	35.66345378354577	37.0	35.0	37.0	33.0	37.0
9	37.29318706870329	39.0	37.0	39.0	34.0	39.0
10-11	37.201601104914516	39.0	37.0	39.0	33.0	39.0
12-13	37.176093168554964	39.0	37.0	39.0	33.0	39.0
14-15	38.38320162016125	40.0	38.0	41.0	33.0	41.0
16-17	38.36463519143839	40.0	38.0	41.0	33.0	41.0
18-19	38.402613359217696	40.0	38.0	41.0	33.5	41.0
20-21	38.376068033054054	40.0	38.0	41.0	34.0	41.0
22-23	38.307767893132585	40.0	38.0	41.0	33.5	41.0
24-25	38.22002781223556	40.0	38.0	41.0	33.0	41.0
26-27	38.066786904620415	40.0	37.0	41.0	33.0	41.0
28-29	37.96631294613343	40.0	37.0	41.0	33.0	41.0
30-31	37.85359370752431	40.0	37.0	41.0	33.0	41.0
32-33	38.015594798289115	40.0	37.0	41.0	33.0	41.0
34-35	38.002598505567605	40.0	37.0	41.0	33.0	41.0
36-37	37.91998488950016	40.0	37.0	41.0	33.0	41.0
38-39	37.79388778041878	40.0	37.0	41.0	33.0	41.0
40-41	37.74325943397544	40.0	37.0	41.0	33.0	41.0
42-43	37.665730605284125	40.0	37.0	41.0	32.5	41.0
44-45	37.66499975988422	40.0	37.0	41.0	32.5	41.0
46-47	37.70508054719203	40.0	37.0	41.0	32.5	41.0
48-49	37.630899473726046	40.0	37.0	41.0	32.0	41.0
50-51	37.5637708321609	40.0	36.5	41.0	32.0	41.0
52-53	37.4249923657602	39.5	36.0	41.0	31.5	41.0
54-55	37.302454897946056	39.0	36.0	41.0	31.0	41.0
56-57	37.133388817698275	39.0	35.5	41.0	31.0	41.0
58-59	36.971855791669626	39.0	35.0	40.0	31.0	41.0
60-61	36.815873068747564	39.0	35.0	40.0	31.0	41.0
62-63	36.59088416191062	38.0	35.0	40.0	31.0	41.0
64-65	36.37287862796211	38.0	35.0	40.0	30.5	41.0
66-67	36.11917719828084	37.5	34.5	40.0	30.0	41.0
68-69	35.830654231422955	37.0	34.0	39.5	30.0	41.0
70-71	35.54697457169986	36.5	34.0	39.0	30.0	41.0
72-73	35.23195770631355	36.0	34.0	39.0	29.0	40.0
74-75	34.89608868329083	35.5	34.0	38.5	29.0	40.0
76-77	33.81160288887578	35.0	32.5	37.0	28.0	39.0
78-79	34.17508194805094	35.0	33.0	37.0	29.0	39.0
80-81	34.07087298964254	35.0	33.0	36.5	29.0	39.0
82-83	33.82682037750215	35.0	33.0	36.0	29.0	37.5
84-85	33.61818544607421	35.0	33.0	36.0	29.0	37.0
86-87	33.43965855080188	35.0	33.0	35.0	29.0	37.0
88-89	33.234994725041986	35.0	33.0	35.0	29.0	36.5
90-91	33.11941526182842	35.0	33.0	35.0	29.0	36.0
92-93	33.06076265807128	34.5	33.0	35.0	29.0	36.0
94-95	33.03050665260206	34.0	33.0	35.0	29.0	35.5
96-97	32.98885872686971	34.0	33.0	35.0	29.0	35.0
98-99	33.07652611585239	34.0	33.0	35.0	29.0	35.0
100-101	32.55726983339191	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.0
17	1.0
18	3.0
19	27.0
20	122.0
21	685.0
22	3317.0
23	10860.0
24	27824.0
25	58004.0
26	100995.0
27	156206.0
28	218725.0
29	284966.0
30	357467.0
31	438052.0
32	541549.0
33	682423.0
34	900105.0
35	1230278.0
36	1869469.0
37	3086400.0
38	3587276.0
39	1678680.0
40	63311.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.940800362220756	22.270222254414975	15.385857478605422	24.403119904758846
2	13.921988969881838	23.077566276779095	40.66183062514093	22.338614128198138
3	16.79744499958782	27.922469016128854	31.768756877686886	23.511329106596442
4	12.205122800929793	19.825212533722034	39.4381512045468	28.531513460801378
5	12.610078128365707	38.4307018439016	36.31055200029758	12.64866802743511
6	28.426353861202795	39.645410930126395	19.25465339073028	12.673581817940532
7	24.79303935757853	33.225177454372826	24.777539332050228	17.20424385599842
8	23.633982619444172	37.29232923936976	22.001609623064354	17.072078518121728
9	25.209245441629456	16.511573799112476	21.78885199724464	36.49032876201342
10-11	23.504494320982843	27.14062968543185	29.947804595603944	19.407071397981362
12-13	24.415393754581448	24.86073348003553	29.761582019813492	20.962290745569533
14-15	21.668931091174993	26.162670251044844	27.193833800894556	24.974564856885603
16-17	21.272895959788578	29.104785598560845	27.886157378930648	21.736161062719926
18-19	21.32016475615782	28.427464898969983	29.400425249514328	20.85194509535787
20-21	22.103175569559657	27.685998567918528	29.13823247281505	21.07259338970676
22-23	21.855192164184352	27.585790460415826	28.999026348865787	21.559991026534036
24-25	21.668913535136312	27.779276344089993	28.997683240824614	21.554126879949077
26-27	21.643227881291295	27.909194651075076	28.90421474139243	21.543362726241195
28-29	21.623888853631158	27.93186207231529	28.927302980123613	21.51694609392994
30-31	21.8366859246744	27.799027121812564	28.83022858121505	21.53405837229799
32-33	21.69657751069841	27.95465037643009	28.753886466853483	21.594885646018014
34-35	21.80572553570038	27.978558203959953	28.774460903863364	21.441255356476304
36-37	21.74853214751239	27.971897894436857	28.721042764422428	21.558527193628326
38-39	21.637064008592503	28.045910806367104	28.7765227125724	21.540502472467995
40-41	21.82975665059853	27.85949587993996	28.685078491507472	21.62566897795404
42-43	21.705345065400657	28.047630763935455	28.441963517269958	21.805060653393927
44-45	21.84647695711169	28.063630192372855	28.29646741174075	21.793425438774705
46-47	21.894952743513592	28.06083136943265	28.08319370573436	21.961022181319404
48-49	21.8067453922381	28.197839323638085	28.0291686585954	21.96624662552842
50-51	21.859849707330046	28.251574119935498	27.934948130238574	21.953628042495886
52-53	21.978903266214925	28.147190219675217	27.827820421824857	22.046086092285
54-55	21.936377056193106	28.07447393728802	27.880656341193227	22.108492665325645
56-57	21.949609388795526	28.127225905429835	27.884900605715146	22.038264100059486
58-59	21.925268387749313	28.156377694169286	27.869492248183093	22.04886166989831
60-61	21.998067089352844	28.107546338236812	27.898500712590625	21.995885859819722
62-63	21.981012299302225	28.201511865991698	27.831365984968	21.98610984973808
64-65	21.946196407047893	28.18400278142136	27.78619255221245	22.083608259318297
66-67	21.994644074936044	28.228297206399393	27.657364883642256	22.119693835022304
68-69	21.971858064167748	28.174754280596336	27.692431708921	22.160955946314914
70-71	21.966204370931735	27.99232296045248	27.798205103125852	22.243267565489933
72-73	21.99544524453013	28.03334813506883	27.67947077776252	22.291735842638513
74-75	21.93218311435453	28.077784318919846	27.70570708246744	22.28432548425818
76-77	22.092301275535554	28.000491500645136	27.639176517757093	22.268030706062216
78-79	22.038509777918513	28.00450760679316	27.62198111468188	22.335001500606445
80-81	22.080570174918112	28.01347089653914	27.60164677651075	22.304312152031997
82-83	22.083281568830337	27.97918896794912	27.679110853186796	22.258418610033747
84-85	22.15699831038434	27.98105386270308	27.65794238043757	22.204005446475
86-87	22.06813079918651	27.985571384179636	27.713449371627735	22.232848445006123
88-89	22.10059608444105	27.91363499299044	27.715562083921323	22.270206838647187
90-91	22.145066655901054	27.879230649155108	27.79183548092665	22.183867214017187
92-93	22.19977280511637	27.80598798077377	27.815586985354233	22.17865222875562
94-95	22.016486428526438	27.83889359046709	27.88101728510883	22.263602695897642
96-97	21.964751106205632	27.884565969820375	27.92958771995815	22.221095204015846
98-99	22.15888332376824	28.47485482131433	28.030336210354605	21.335925644562824
100-101	22.728142857021457	31.108231908968616	25.238185207541914	20.92544002646801
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3370.0
1	3035.5
2	4256.0
3	6118.0
4	8123.5
5	12617.0
6	16344.0
7	19009.5
8	20620.0
9	20235.5
10	18785.5
11	17486.0
12	19441.0
13	25908.0
14	40623.5
15	60598.5
16	76373.0
17	84256.0
18	85767.0
19	83586.5
20	79717.0
21	77161.5
22	78999.0
23	88328.5
24	105615.5
25	131467.5
26	163637.5
27	196649.5
28	231168.5
29	266857.0
30	300735.5
31	335601.5
32	372174.0
33	406872.0
34	439676.5
35	471649.5
36	505382.5
37	536751.0
38	561687.5
39	580687.5
40	598660.5
41	615477.0
42	624517.5
43	629851.5
44	636246.5
45	643678.0
46	646506.0
47	642850.0
48	634975.0
49	620759.5
50	602331.0
51	579660.5
52	550833.0
53	517950.5
54	483119.0
55	446076.5
56	408356.0
57	367751.0
58	324128.0
59	279272.0
60	233101.5
61	189341.0
62	149635.0
63	115515.0
64	86028.0
65	63523.5
66	47594.5
67	35871.0
68	27033.5
69	19498.5
70	13956.0
71	10071.5
72	6988.0
73	4710.0
74	3166.0
75	2130.0
76	1426.0
77	942.0
78	633.5
79	416.0
80	300.0
81	209.5
82	142.5
83	94.0
84	57.5
85	37.0
86	28.5
87	23.0
88	16.5
89	13.0
90	9.0
91	6.0
92	5.5
93	4.0
94	2.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004118522177490132
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.393302851475172E-4
20-21	0.0011604268932691646
22-23	0.002595725382977377
24-25	2.6159957874619834E-5
26-27	0.007880628982146597
28-29	1.734699281369729E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.034664505110678576
38-39	0.015141669390242606
40-41	0.024572058503018284
42-43	0.0012171452986016798
44-45	0.00541508306008329
46-47	0.004506183230962449
48-49	0.002571293035049348
50-51	0.002409678739651808
52-53	1.7827912720814744E-4
54-55	0.002557977127223313
56-57	0.01470579901096236
58-59	0.010005392855364044
60-61	0.0035350990825583255
62-63	0.0010419884227589912
64-65	0.003159228766346168
66-67	2.5291932126570945E-4
68-69	4.84903800322977E-4
70-71	2.1997199599368145E-4
72-73	3.9775353576044925E-6
74-75	0.0
76-77	0.006302462133318572
78-79	3.141755685833691E-4
80-81	0.0
82-83	1.233837316167281E-4
84-85	0.0018773154906364033
86-87	0.005360514528811868
88-89	0.07864095263981549
90-91	0.002959852318756905
92-93	0.0013831227398333678
94-95	0.0
96-97	0.017132352075576493
98-99	3.02400378605274E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	408.0
20-21	1381.0
22-23	2878.0
24-25	5951.0
26-27	7465.0
28-29	13108.0
30-31	36761.0
32-33	47751.0
34-35	67342.0
36-37	103104.0
38-39	111654.0
40-41	119788.0
42-43	133816.0
44-45	157675.0
46-47	158397.0
48-49	144416.0
50-51	131771.0
52-53	122988.0
54-55	132278.0
56-57	139588.0
58-59	143186.0
60-61	141238.0
62-63	138432.0
64-65	148041.0
66-67	158497.0
68-69	159730.0
70-71	160091.0
72-73	154398.0
74-75	158365.0
76-77	159028.0
78-79	170131.0
80-81	173067.0
82-83	167040.0
84-85	166695.0
86-87	176866.0
88-89	186454.0
90-91	201108.0
92-93	208327.0
94-95	256239.0
96-97	327687.0
98-99	1234742.0
100-101	8868867.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.12903384656383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.34494491549498	75.16503894815058
2	7.241284665797389	12.184045654829442
3	1.863839951488661	4.704091630901023
4	0.6971256586607093	2.3459403253109965
5	0.3397497952484805	1.429141101191127
6	0.17016584118578829	0.8589532687588913
7	0.0986137365047474	0.5807394863300814
8	0.06601056264058909	0.44427238868966734
9	0.03879159256119441	0.2937149283188555
>10	0.13593884457703898	1.7469491936295705
>50	0.00289327076357475	0.1579834080306665
>100	6.274826811727588E-4	0.08153249344028786
>500	1.368239569008705E-5	0.007597172418748523
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3074673579333753E-5	0.0	6.537336789666876E-6	0.0
3	0.0	1.3074673579333753E-5	0.0	1.3074673579333753E-5	0.0
4	0.0	1.961201036900063E-5	0.0	1.961201036900063E-5	0.0
5	6.537336789666876E-6	1.961201036900063E-5	0.0	1.961201036900063E-5	6.537336789666876E-6
6	6.537336789666876E-6	1.961201036900063E-5	0.0	1.961201036900063E-5	6.537336789666876E-6
7	6.537336789666876E-6	1.961201036900063E-5	0.0	1.961201036900063E-5	6.537336789666876E-6
8	6.537336789666876E-6	2.6149347158667505E-5	0.0	1.961201036900063E-5	6.537336789666876E-6
9	6.537336789666876E-6	6.537336789666876E-5	0.0	3.268668394833438E-5	6.537336789666876E-6
10-11	6.537336789666876E-6	6.537336789666876E-5	0.0	5.229869431733501E-5	1.961201036900063E-5
12-13	6.537336789666876E-6	6.537336789666876E-5	0.0	5.8836031107001886E-5	1.961201036900063E-5
14-15	9.806005184500314E-6	1.1440339381917034E-4	0.0	5.8836031107001886E-5	3.268668394833438E-5
16-17	1.961201036900063E-5	1.1767206221400377E-4	0.0	8.171670987083596E-5	3.922402073800126E-5
18-19	1.961201036900063E-5	1.2747806739850408E-4	0.0	1.3074673579333753E-4	5.8836031107001886E-5
20-21	1.961201036900063E-5	1.372840725830044E-4	0.0	1.9285143529517286E-4	7.517937308116907E-5
22-23	1.961201036900063E-5	1.438214093726713E-4	0.0	3.693595286161785E-4	8.49853782656694E-5
24-25	1.961201036900063E-5	1.634334197416719E-4	0.0	9.904065236345317E-4	9.479138345016971E-5
26-27	2.2880678763834067E-5	1.7650809332100565E-4	0.0	0.0032588623896489376	9.806005184500315E-5
28-29	2.6149347158667505E-5	1.797767617158391E-4	0.0	0.017261837793115388	9.806005184500315E-5
30-31	2.6149347158667505E-5	1.8958276690033942E-4	0.0	0.02634219859396268	9.806005184500315E-5
32-33	2.6149347158667505E-5	1.8958276690033942E-4	0.0	0.034180465404773264	9.806005184500315E-5
34-35	3.268668394833438E-5	2.0919477726934004E-4	0.0	0.04407472463593408	1.111347254243369E-4
36-37	3.268668394833438E-5	2.2880678763834066E-4	0.0	0.057685459832020516	1.111347254243369E-4
38-39	3.268668394833438E-5	2.3534412442800755E-4	0.0	0.08138657436295778	1.1440339381917034E-4
40-41	3.268668394833438E-5	2.5495613479700817E-4	0.0	0.12576528515961138	1.1767206221400377E-4
42-43	3.268668394833438E-5	2.6149347158667505E-4	0.0	0.1456420576685935	1.1767206221400377E-4
44-45	3.268668394833438E-5	2.9418015553500947E-4	0.0	0.1735597544288659	1.2420939900367064E-4
46-47	3.268668394833438E-5	3.2032950269367694E-4	0.0	0.20471670156841823	1.3074673579333753E-4
48-49	3.268668394833438E-5	3.2032950269367694E-4	0.0	0.22998677692887554	1.4709007776750473E-4
50-51	3.268668394833438E-5	3.497475182471779E-4	0.0	0.2528282316719716	1.536274145571716E-4
52-53	3.268668394833438E-5	3.595535234316782E-4	0.0	0.2827496221582769	1.699707565313388E-4
54-55	3.268668394833438E-5	3.595535234316782E-4	0.0	0.34329516683577666	1.8304543011067254E-4
56-57	3.268668394833438E-5	3.6609086022134507E-4	0.0	0.4066844530167816	1.8304543011067254E-4
58-59	3.922402073800126E-5	3.6609086022134507E-4	0.0	0.45885566926671806	1.8631409850550598E-4
60-61	3.922402073800126E-5	3.693595286161785E-4	0.0	0.4949940670399965	1.8958276690033942E-4
62-63	3.922402073800126E-5	3.857028705903457E-4	0.0	0.525167144992704	2.0265744047967316E-4
64-65	3.922402073800126E-5	4.641509120663482E-4	0.0	0.5393368224843069	2.059261088745066E-4
66-67	3.922402073800126E-5	4.77225585645682E-4	0.0	0.5470901039168519	2.1573211405900692E-4
68-69	3.922402073800126E-5	4.903002592250157E-4	0.0	0.5550950728157991	2.222694508486738E-4
70-71	3.922402073800126E-5	4.903002592250157E-4	0.0	0.5622469192636945	2.2553811924350722E-4
72-73	3.922402073800126E-5	4.935689276198492E-4	0.0	0.5680912983536568	2.5495613479700817E-4
74-75	3.922402073800126E-5	5.099122695940163E-4	0.0	0.5733538544693385	2.6476213998150846E-4
76-77	3.922402073800126E-5	5.458676219371842E-4	0.0	0.5783516484450388	2.6803080837634193E-4
78-79	3.922402073800126E-5	5.622109639113514E-4	0.0	0.5825714993427689	2.6803080837634193E-4
80-81	3.922402073800126E-5	5.622109639113514E-4	0.0	0.5849870452865508	2.90911487140176E-4
82-83	3.922402073800126E-5	5.622109639113514E-4	0.0	0.5865625434528605	3.301355078781773E-4
84-85	3.922402073800126E-5	5.687483007010182E-4	0.0	0.5880203695569561	3.334041762730107E-4
86-87	3.922402073800126E-5	5.81822974280352E-4	0.0	0.5882459076761997	3.334041762730107E-4
88-89	3.922402073800126E-5	6.014349846493526E-4	0.0	0.5882687883549635	3.43210181457511E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16865	0.0	46.472687	1
GTATCAA	29360	0.0	33.864944	1
TCAACGC	36440	0.0	26.570463	4
ATCAACG	36855	0.0	26.271269	3
CAACGCA	37385	0.0	25.876394	5
AACGCAG	38625	0.0	25.110807	6
TATCAAC	39210	0.0	24.91919	2
ACGCAGA	45245	0.0	21.566483	7
CGCAGAG	45535	0.0	21.410715	8
GTACATG	39285	0.0	19.779873	1
TACATGG	39990	0.0	19.106089	2
AGAGTAC	47980	0.0	18.353233	10-11
ACATGGG	40500	0.0	18.170866	3
GCAGAGT	54050	0.0	17.905796	9
GAGTACT	31565	0.0	17.117317	12-13
CATGGGG	29965	0.0	16.666754	4
CAGAGTA	52545	0.0	16.419579	10-11
AGTACTT	35430	0.0	15.918723	12-13
GTGGTAT	8520	0.0	15.553291	1
GTACTTT	36740	0.0	14.689983	14-15
>>END_MODULE