FastQCFastQC Report
Fri 27 May 2016
SRR2139808_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139808_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21379313
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA219300.045.7802051
GTATCAA384650.033.9165341
ATCAACG465050.027.172643
TCAACGC466750.027.046574
CAACGCA477650.026.4293655
AACGCAG496700.025.5006076
TATCAAC504400.025.438052
ACGCAGA569350.022.2170647
CGCAGAG577350.021.894618
GTACATG515100.019.4660221
TACATGG517900.018.6036112
ACATGGG523400.017.9725883
AGAGTAC631300.017.90295610-11
GCAGAGT704000.017.770099
GAGTACT412100.016.87201112-13
CAGAGTA688850.016.24198710-11
CATGGGG375450.015.9470734
AGTACTT463550.015.63608612-13
GTACTTT480600.014.52050814-15
TATACTG130950.013.5556815
Produced by FastQC (version 0.11.5)