##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139808_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21379313
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03750929695449	33.0	31.0	34.0	30.0	34.0
2	32.200130097725776	33.0	31.0	34.0	30.0	34.0
3	32.25201703160434	34.0	31.0	34.0	30.0	34.0
4	35.89386600027793	37.0	35.0	37.0	35.0	37.0
5	35.77115373164704	37.0	35.0	37.0	35.0	37.0
6	35.782235191561114	37.0	35.0	37.0	33.0	37.0
7	35.70313695299751	37.0	35.0	37.0	33.0	37.0
8	35.683573134459465	37.0	35.0	37.0	33.0	37.0
9	37.316338041358016	39.0	37.0	39.0	34.0	39.0
10-11	37.22999511256512	39.0	37.0	39.0	33.0	39.0
12-13	37.20766282340317	39.0	37.0	39.0	33.0	39.0
14-15	38.42707111308955	40.0	38.0	41.0	33.0	41.0
16-17	38.411275469889986	40.0	38.0	41.0	33.0	41.0
18-19	38.448276705617246	40.0	38.0	41.0	33.5	41.0
20-21	38.42224667401422	40.0	38.0	41.0	34.0	41.0
22-23	38.35593845398574	40.0	38.0	41.0	34.0	41.0
24-25	38.27301346010785	40.0	38.0	41.0	33.0	41.0
26-27	38.12696351209716	40.0	38.0	41.0	33.0	41.0
28-29	38.030495565771204	40.0	37.0	41.0	33.0	41.0
30-31	37.921980965991914	40.0	37.0	41.0	33.0	41.0
32-33	38.08185823436462	40.0	37.0	41.0	33.0	41.0
34-35	38.06997390482199	40.0	37.0	41.0	33.0	41.0
36-37	37.98971439331163	40.0	37.0	41.0	33.0	41.0
38-39	37.86687073702806	40.0	37.0	41.0	33.0	41.0
40-41	37.82065319005639	40.0	37.0	41.0	33.0	41.0
42-43	37.74579353501324	40.0	37.0	41.0	33.0	41.0
44-45	37.749400076426504	40.0	37.0	41.0	33.0	41.0
46-47	37.79163753498402	40.0	37.0	41.0	33.0	41.0
48-49	37.72410044435527	40.0	37.0	41.0	32.0	41.0
50-51	37.665816148432626	40.0	37.0	41.0	32.0	41.0
52-53	37.535117725716	40.0	36.5	41.0	32.0	41.0
54-55	37.42190631093948	39.0	36.0	41.0	31.5	41.0
56-57	37.26361402154663	39.0	36.0	41.0	31.0	41.0
58-59	37.11265850480024	39.0	35.5	41.0	31.0	41.0
60-61	36.968414899791284	39.0	35.0	40.0	31.0	41.0
62-63	36.75509657236307	38.5	35.0	40.0	31.0	41.0
64-65	36.55031228550272	38.0	35.0	40.0	31.0	41.0
66-67	36.30630360273457	38.0	35.0	40.0	30.5	41.0
68-69	36.02334930329206	37.0	34.0	40.0	30.0	41.0
70-71	35.74363632607346	37.0	34.0	39.0	30.0	41.0
72-73	35.42831854287975	36.0	34.0	39.0	30.0	40.5
74-75	35.088028817793386	36.0	34.0	39.0	29.0	40.0
76-77	33.98858378556017	35.0	32.5	37.0	28.5	39.0
78-79	34.33838672079774	35.0	33.0	37.0	29.0	39.0
80-81	34.21955945597719	35.0	33.0	37.0	29.0	39.0
82-83	33.959909185583136	35.0	33.0	36.0	29.0	38.5
84-85	33.73552192277842	35.0	33.0	36.0	29.0	37.0
86-87	33.54387240222816	35.0	33.0	35.5	29.0	37.0
88-89	33.3278487422583	35.0	33.0	35.0	29.0	36.5
90-91	33.201372760577584	35.0	33.0	35.0	29.0	36.0
92-93	33.13196237792283	35.0	33.0	35.0	29.0	36.0
94-95	33.09597185774562	34.5	33.0	35.0	29.0	36.0
96-97	33.05028794480536	34.0	33.0	35.0	29.0	35.0
98-99	33.13155102584136	34.0	33.0	35.0	29.0	35.0
100-101	32.61382355925493	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	2.0
18	5.0
19	20.0
20	138.0
21	829.0
22	4180.0
23	14028.0
24	36428.0
25	76209.0
26	134549.0
27	209400.0
28	293981.0
29	386124.0
30	484964.0
31	599356.0
32	738075.0
33	930661.0
34	1222325.0
35	1666549.0
36	2518909.0
37	4200456.0
38	5236076.0
39	2521477.0
40	104571.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.55759598075017	22.546374619240574	15.560602906183188	24.335426493826066
2	13.592759853351472	23.170474096780207	41.2128150285086	22.02395102135972
3	16.52293504473226	28.17086311426377	31.978960221967846	23.327241619036123
4	11.88297771775922	20.060686702140522	40.09918840703628	27.95714717306398
5	12.361173626112308	38.60680181818751	36.660583995379085	12.37144056032109
6	27.9360052402058	40.219061295374644	19.506665158043198	12.338268306376357
7	24.456978575504273	33.40277585159075	25.16766090659695	16.972584666308034
8	23.205231150318067	37.76551192267029	22.25094884947893	16.778308077532706
9	24.97902528486299	16.735771631202557	22.13505176709841	36.15015131683605
10-11	23.099194066712993	27.452446671228397	30.334791393904943	19.11356786815367
12-13	24.100781442322305	25.095450915564964	30.075084732610446	20.728682909502282
14-15	21.32507952898206	26.444671070581173	27.528889726250792	24.701359674185976
16-17	20.96100796129417	29.38194739933879	28.24468681477277	21.41235782459427
18-19	20.996476411899668	28.778752564729793	29.674083010069392	20.55068801330115
20-21	21.745459304757965	28.056596282273027	29.42906763443025	20.768876778538758
22-23	21.4683385789357	27.996922952122745	29.269981689006148	21.264756779935407
24-25	21.333221565036283	28.16432410235755	29.258110087623784	21.244344244982383
26-27	21.347030264151282	28.254606806456835	29.179649816798786	21.2187131125931
28-29	21.302449758436165	28.282496525355484	29.207345065321068	21.207708650887284
30-31	21.541312223809314	28.158066753578016	29.140772752083148	21.159848270529523
32-33	21.342945985814836	28.29685800913053	29.06010042292303	21.300095582131608
34-35	21.467349465465933	28.32819917187821	29.07364645757712	21.130804905078737
36-37	21.439648574942012	28.308845097653613	29.030401395085452	21.221104932318926
38-39	21.3214025277504	28.386051966893678	29.074906743323336	21.217638762032585
40-41	21.50648567269896	28.245285627091228	28.978396818913314	21.2698318812965
42-43	21.423256325626173	28.374588880176567	28.749081598769134	21.453073195428132
44-45	21.494571762669214	28.41378521055955	28.636704973469772	21.45493805330146
46-47	21.56025554333774	28.373803810355035	28.418253129415273	21.64768751689195
48-49	21.499279057478503	28.492380355609097	28.416291053380334	21.592049533532066
50-51	21.5483920846904	28.550668905964837	28.28356233758352	21.617376671761246
52-53	21.61341260127115	28.494822412399497	28.199199775987875	21.69256521034147
54-55	21.592842874033842	28.409651191767633	28.271807620753197	21.725698313445328
56-57	21.6295449503098	28.451259827049768	28.217732714487347	21.70146250815308
58-59	21.60833833550107	28.46421464086316	28.251666253279485	21.67578077035629
60-61	21.67648340062504	28.461966286808664	28.221609610236364	21.639940702329927
62-63	21.619727756684874	28.544284000528908	28.18925296092812	21.646735281858103
64-65	21.56714941047808	28.491255333577115	28.158293507307654	21.78330174863715
66-67	21.64642123979965	28.558903839028993	28.043829322859633	21.750845598311724
68-69	21.61123474851755	28.52519605851991	28.06068131619827	21.80288787676427
70-71	21.606404649688123	28.357689185016184	28.13027417971235	21.905631985583344
72-73	21.62466946790621	28.371904145008653	28.061548802007273	21.94187758507787
74-75	21.609195953633762	28.41515114372542	28.040412841117394	21.935240061523423
76-77	21.71362948358891	28.36755486613818	27.98268872738032	21.936126922892587
78-79	21.707617959160732	28.32704319294786	27.983028330807908	21.982310517083498
80-81	21.718923113774274	28.342980917854497	27.95154719424969	21.986548774121534
82-83	21.75784749854848	28.31827549044374	28.034889439990877	21.888987571016905
84-85	21.79846238858309	28.295913517617656	28.03238522392812	21.873238869871138
86-87	21.695848413848264	28.334893017621678	28.06910363146793	21.900154937062137
88-89	21.738425163517793	28.323757824822376	28.06002436895372	21.877792642706115
90-91	21.79361428063267	28.27353373749656	28.115433027922514	21.817418953948255
92-93	21.843435632249744	28.17667688210322	28.116209308241984	21.863678177405056
94-95	21.675203817522927	28.203940726782083	28.224792904333118	21.89606255136187
96-97	21.605876352657955	28.277468319263143	28.258578790453758	21.858076537625145
98-99	21.79674704695668	28.78860996587561	28.410888215957268	21.00375477121044
100-101	22.31350427014202	31.50216235502295	25.63817215074981	20.546161224085218
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4073.0
1	3741.5
2	5341.5
3	7664.0
4	10297.5
5	16080.0
6	20747.0
7	24182.5
8	26157.0
9	25449.0
10	23751.5
11	22412.0
12	25254.0
13	33922.5
14	53306.0
15	80088.5
16	101354.0
17	112051.0
18	114356.0
19	111979.5
20	108208.5
21	106613.5
22	111286.0
23	126705.5
24	153455.5
25	191235.5
26	239532.5
27	290550.0
28	341930.0
29	395902.0
30	449297.0
31	501060.5
32	552974.0
33	604989.0
34	653779.5
35	700560.0
36	750740.5
37	794751.5
38	825691.5
39	850840.0
40	875826.5
41	897156.0
42	909365.5
43	917096.0
44	920993.0
45	920265.0
46	912587.0
47	898432.5
48	879065.0
49	849730.5
50	815750.5
51	774486.0
52	723322.5
53	669425.5
54	614427.0
55	559359.0
56	505341.0
57	450319.0
58	393702.5
59	334835.0
60	274727.0
61	219110.0
62	171193.0
63	131180.0
64	97348.5
65	72117.5
66	54290.5
67	41055.0
68	31116.5
69	22651.0
70	16188.5
71	11584.0
72	8111.0
73	5512.0
74	3730.0
75	2518.5
76	1681.0
77	1105.5
78	755.5
79	506.5
80	352.0
81	256.0
82	185.0
83	130.0
84	92.0
85	66.0
86	47.0
87	36.0
88	32.5
89	27.0
90	19.0
91	12.5
92	8.5
93	6.0
94	3.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003980483376617387
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.215321932935824E-4
20-21	0.0012208535824685426
22-23	0.0026828832260690732
24-25	1.1697769045939081E-5
26-27	0.0077911554681425405
28-29	2.2244044280071622E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.03530063473876156
38-39	0.015885721255030648
40-41	0.024844564153953485
42-43	0.0013508922207346268
44-45	0.005570545807453303
46-47	0.004671380019974347
48-49	0.002731476956736548
50-51	0.0023042179654208655
52-53	2.514871655430271E-4
54-55	0.0027104777565462716
56-57	0.014599131659370247
58-59	0.010052941663066755
60-61	0.0036058664043642852
62-63	0.0011724885787621068
64-65	0.003389295930438187
66-67	3.0486386617086E-4
68-69	5.783850563809752E-4
70-71	2.090642626469314E-4
72-73	0.0
74-75	2.8551271373838644E-6
76-77	0.006327267124991275
78-79	3.454393429298725E-4
80-81	5.934905129651267E-6
82-83	9.629146366846344E-5
84-85	0.0018853165597512188
86-87	0.005535038682154627
88-89	0.07874926688892996
90-91	0.002873721034488484
92-93	0.001391310227397954
94-95	0.0
96-97	0.01739477680377975
98-99	5.319022939456611E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	490.0
20-21	1808.0
22-23	3651.0
24-25	7200.0
26-27	9297.0
28-29	16796.0
30-31	48248.0
32-33	63040.0
34-35	87926.0
36-37	137060.0
38-39	148394.0
40-41	160068.0
42-43	180210.0
44-45	209617.0
46-47	212329.0
48-49	192839.0
50-51	177614.0
52-53	162288.0
54-55	177372.0
56-57	186644.0
58-59	192756.0
60-61	194129.0
62-63	187362.0
64-65	200161.0
66-67	213989.0
68-69	216529.0
70-71	216301.0
72-73	209655.0
74-75	214753.0
76-77	214667.0
78-79	228303.0
80-81	234162.0
82-83	228073.0
84-85	226034.0
86-87	239828.0
88-89	253769.0
90-91	275571.0
92-93	285581.0
94-95	353590.0
96-97	455711.0
98-99	1737490.0
100-101	1.2618008E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.25928500728105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.14555483886552	75.11340712300172
2	7.435410236352135	12.53004700501699
3	1.8922635642003158	4.783223248945436
4	0.7095361686422542	2.3914004102640773
5	0.33897851088150577	1.4281043479854256
6	0.16581008321498084	0.8382623435215234
7	0.09598293622132109	0.5661217505235581
8	0.06015793284074963	0.4055091526942076
9	0.0394479619694603	0.29914713634870255
>10	0.11436759999438675	1.4384939542285826
>50	0.001990879272500651	0.10815300374823851
>100	4.434606671993796E-4	0.062111617200866415
>500	3.732075693251842E-5	0.019451231806733797
>1k	1.8506120713934273E-5	0.016567674713943242
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.677418773933475E-6	4.677418773933475E-6
2	0.0	1.4032256321800425E-5	0.0	1.87096750957339E-5	4.677418773933475E-6
3	0.0	1.4032256321800425E-5	0.0	2.806451264360085E-5	4.677418773933475E-6
4	0.0	3.74193501914678E-5	0.0	3.74193501914678E-5	4.677418773933475E-6
5	0.0	3.74193501914678E-5	0.0	3.74193501914678E-5	4.677418773933475E-6
6	0.0	4.2096768965401274E-5	0.0	3.74193501914678E-5	4.677418773933475E-6
7	0.0	4.2096768965401274E-5	0.0	5.1451606513268224E-5	4.677418773933475E-6
8	9.35483754786695E-6	4.677418773933475E-5	0.0	5.1451606513268224E-5	9.35483754786695E-6
9	1.87096750957339E-5	9.35483754786695E-5	0.0	1.0290321302653645E-4	9.35483754786695E-6
10-11	2.806451264360085E-5	9.35483754786695E-5	0.0	1.496774007658712E-4	9.35483754786695E-6
12-13	3.74193501914678E-5	9.588708486563623E-5	0.0	1.7072578524857183E-4	9.35483754786695E-6
14-15	3.74193501914678E-5	1.239515975092371E-4	0.0	2.1048384482700636E-4	9.35483754786695E-6
16-17	4.677418773933475E-5	1.4032256321800423E-4	0.0	2.5959674195330783E-4	9.35483754786695E-6
18-19	5.3790315900234965E-5	1.4733869137890444E-4	0.0	3.3443544233624343E-4	9.35483754786695E-6
20-21	6.548386283506865E-5	1.5903223831373813E-4	0.0	3.999193051713121E-4	9.35483754786695E-6
22-23	6.548386283506865E-5	1.7306449463553856E-4	0.0	5.61290252872017E-4	1.87096750957339E-5
24-25	7.48387003829356E-5	1.8475804157037223E-4	0.0	0.0011787095310312357	2.806451264360085E-5
26-27	9.35483754786695E-5	1.9645158850520593E-4	0.0	0.0030870963907960935	2.806451264360085E-5
28-29	9.35483754786695E-5	2.0346771666610616E-4	0.0	0.01653467536585483	2.806451264360085E-5
30-31	9.822579425260297E-5	2.1983868237487333E-4	0.0	0.02511072268786186	2.806451264360085E-5
32-33	1.122580505744034E-4	2.245161011488068E-4	0.0	0.03236539920623268	2.806451264360085E-5
34-35	1.1927417873530361E-4	2.689515795011748E-4	0.0	0.0420102367180835	3.2741931417534324E-5
36-37	1.2862901628317056E-4	2.993548015317424E-4	0.0	0.055212251207510735	3.2741931417534324E-5
38-39	1.3330643505710403E-4	3.1806447662747627E-4	0.0	0.0782345064128113	3.2741931417534324E-5
40-41	1.5435481953980466E-4	3.437902798841104E-4	0.0	0.12351192014448734	3.2741931417534324E-5
42-43	1.5435481953980466E-4	3.508064080450106E-4	0.0	0.14374175634174963	3.2741931417534324E-5
44-45	1.5435481953980466E-4	3.858870488495117E-4	0.0	0.1727323979025893	3.2741931417534324E-5
46-47	1.566935289267714E-4	4.18628980267046E-4	0.0	0.2043587649425405	3.2741931417534324E-5
48-49	1.637096570876716E-4	4.2330639904097943E-4	0.0	0.23076981004955585	3.2741931417534324E-5
50-51	1.7774191340947203E-4	4.700805867803142E-4	0.0	0.25494738769201797	3.2741931417534324E-5
52-53	1.8008062279643877E-4	4.817741337151479E-4	0.0	0.2881056093804324	3.2741931417534324E-5
54-55	1.8241933218340553E-4	4.911289712630149E-4	0.0	0.3542630205189474	3.2741931417534324E-5
56-57	1.87096750957339E-4	5.098386463587487E-4	0.0	0.4228409958729731	3.2741931417534324E-5
58-59	1.987902978921727E-4	5.098386463587487E-4	0.0	0.47916413403929303	3.2741931417534324E-5
60-61	2.058064260530729E-4	5.121773557457155E-4	0.0	0.5139267103671665	3.2741931417534324E-5
62-63	2.1048384482700636E-4	5.215321932935824E-4	0.0	0.5343834013749647	3.2741931417534324E-5
64-65	2.1983868237487333E-4	5.753225091938174E-4	0.0	0.5489605769839283	3.2741931417534324E-5
66-67	2.2217739176184006E-4	5.753225091938174E-4	0.0	0.5566221889356313	3.2741931417534324E-5
68-69	2.31532229309707E-4	5.823386373547176E-4	0.0	0.5642908170154953	3.2741931417534324E-5
70-71	2.3854835747060723E-4	5.96370893676518E-4	4.677418773933475E-6	0.5714238806457439	3.2741931417534324E-5
72-73	2.5024190440544095E-4	6.010483124504515E-4	4.677418773933475E-6	0.5771186380030079	3.2741931417534324E-5
74-75	2.5491932317937436E-4	6.15080568772252E-4	4.677418773933475E-6	0.5819925083654466	3.2741931417534324E-5
76-77	2.572580325663411E-4	6.431450814158528E-4	4.677418773933475E-6	0.586386943303557	3.2741931417534324E-5
78-79	2.572580325663411E-4	6.548386283506865E-4	4.677418773933475E-6	0.5906270234221277	3.2741931417534324E-5
80-81	2.572580325663411E-4	6.548386283506865E-4	4.677418773933475E-6	0.5928160554083286	3.2741931417534324E-5
82-83	2.642741607272413E-4	6.618547565115867E-4	4.677418773933475E-6	0.5944812164918489	3.2741931417534324E-5
84-85	2.7129028888814153E-4	6.712095940594536E-4	4.677418773933475E-6	0.5959850066276686	3.2741931417534324E-5
86-87	2.7129028888814153E-4	6.875805597682208E-4	4.677418773933475E-6	0.5962446033696218	3.2741931417534324E-5
88-89	2.7362899827510824E-4	7.03951525476988E-4	4.677418773933475E-6	0.5962820227198133	3.2741931417534324E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	21930	0.0	45.780205	1
GTATCAA	38465	0.0	33.916534	1
ATCAACG	46505	0.0	27.17264	3
TCAACGC	46675	0.0	27.04657	4
CAACGCA	47765	0.0	26.429365	5
AACGCAG	49670	0.0	25.500607	6
TATCAAC	50440	0.0	25.43805	2
ACGCAGA	56935	0.0	22.217064	7
CGCAGAG	57735	0.0	21.89461	8
GTACATG	51510	0.0	19.466022	1
TACATGG	51790	0.0	18.603611	2
ACATGGG	52340	0.0	17.972588	3
AGAGTAC	63130	0.0	17.902956	10-11
GCAGAGT	70400	0.0	17.77009	9
GAGTACT	41210	0.0	16.872011	12-13
CAGAGTA	68885	0.0	16.241987	10-11
CATGGGG	37545	0.0	15.947073	4
AGTACTT	46355	0.0	15.636086	12-13
GTACTTT	48060	0.0	14.520508	14-15
TATACTG	13095	0.0	13.555681	5
>>END_MODULE