FastQCFastQC Report
Fri 27 May 2016
SRR2139807_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139807_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13508161
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158950.047.198281
GTATCAA275600.035.281721
GACCGAG125450.027.80791590-91
ACCGAGG129250.027.37422492-93
AGACCGA125150.027.27619490-91
TCAACGC347450.027.2293014
GAGACCG126800.027.18135688-89
ATCAACG349800.027.1178593
CGAGACC127500.026.45951388-89
CAACGCA357600.026.4331255
TATCAAC368450.026.0739332
AACGCAG366450.025.908486
ACGAGAC134400.025.57342386-87
GCCCACG134700.024.4015382-83
CACGAGA137550.024.25092786-87
CCCACGA136950.023.83816384-85
CCACGAG143200.023.38962684-85
ATCTCCG138800.022.81293574-75
CGAGCCC142950.022.3241380-81
TCCGAGC141350.022.29061178-79
Produced by FastQC (version 0.11.5)