##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139807_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13508161
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00741151959915	33.0	31.0	34.0	30.0	34.0
2	32.16925434927819	33.0	31.0	34.0	30.0	34.0
3	32.22001055510073	34.0	31.0	34.0	30.0	34.0
4	35.8697512562961	37.0	35.0	37.0	35.0	37.0
5	35.74380265381794	37.0	35.0	37.0	35.0	37.0
6	35.757454030937296	37.0	35.0	37.0	33.0	37.0
7	35.67508878521658	37.0	35.0	37.0	33.0	37.0
8	35.65746188544836	37.0	35.0	37.0	33.0	37.0
9	37.28610652478898	39.0	37.0	39.0	34.0	39.0
10-11	37.19467305727257	39.0	37.0	39.0	33.0	39.0
12-13	37.16819820995619	39.0	37.0	39.0	33.0	39.0
14-15	38.367860954574056	40.0	38.0	41.0	33.0	41.0
16-17	38.35145453922262	40.0	38.0	41.0	33.0	41.0
18-19	38.39184556654307	40.0	38.0	41.0	33.5	41.0
20-21	38.36611296773644	40.0	38.0	41.0	34.0	41.0
22-23	38.297675938153674	40.0	38.0	41.0	33.5	41.0
24-25	38.208532968089145	40.0	38.0	41.0	33.0	41.0
26-27	38.05579162732032	40.0	37.0	41.0	33.0	41.0
28-29	37.956398643046555	40.0	37.0	41.0	33.0	41.0
30-31	37.8415897023028	40.0	37.0	41.0	33.0	41.0
32-33	38.008926839950334	40.0	37.0	41.0	33.0	41.0
34-35	37.99770525605041	40.0	37.0	41.0	33.0	41.0
36-37	37.917438661445146	40.0	37.0	41.0	33.0	41.0
38-39	37.791368852588484	40.0	37.0	41.0	33.0	41.0
40-41	37.74394329420987	40.0	37.0	41.0	33.0	41.0
42-43	37.66657928414898	40.0	37.0	41.0	32.5	41.0
44-45	37.66571173117643	40.0	37.0	41.0	32.5	41.0
46-47	37.702694362345795	40.0	37.0	41.0	32.5	41.0
48-49	37.62689088850776	40.0	37.0	41.0	32.0	41.0
50-51	37.56283779153367	40.0	36.5	41.0	32.0	41.0
52-53	37.42430977411959	39.5	36.0	41.0	31.5	41.0
54-55	37.30311524487218	39.0	36.0	41.0	31.0	41.0
56-57	37.13476152970307	39.0	35.5	41.0	31.0	41.0
58-59	36.97191063606285	39.0	35.0	40.0	31.0	41.0
60-61	36.82077526162606	39.0	35.0	40.0	31.0	41.0
62-63	36.59495409598036	38.0	35.0	40.0	31.0	41.0
64-65	36.37903164478642	38.0	35.0	40.0	30.5	41.0
66-67	36.13014929847588	37.5	34.5	40.0	30.0	41.0
68-69	35.84093353172403	37.0	34.0	39.5	30.0	41.0
70-71	35.559020427641414	36.5	34.0	39.0	30.0	41.0
72-73	35.24070010829733	36.0	34.0	39.0	29.0	40.0
74-75	34.906098887155984	35.5	34.0	38.5	29.0	40.0
76-77	33.82389336801808	35.0	32.5	37.0	28.0	39.0
78-79	34.1837068274981	35.0	33.0	37.0	29.0	39.0
80-81	34.068978061965566	35.0	33.0	36.5	29.0	39.0
82-83	33.814365750666305	35.0	33.0	36.0	29.0	37.5
84-85	33.611453497035484	35.0	33.0	36.0	29.0	37.0
86-87	33.43814193491309	35.0	33.0	35.0	29.0	37.0
88-89	33.23827352738395	35.0	33.0	35.0	29.0	36.0
90-91	33.11716424994825	35.0	33.0	35.0	29.0	36.0
92-93	33.06449460299767	35.0	33.0	35.0	29.0	36.0
94-95	33.03206338697893	34.0	33.0	35.0	29.0	35.5
96-97	32.9894867501716	34.0	33.0	35.0	29.0	35.0
98-99	33.08164573308589	34.0	33.0	35.0	29.0	35.0
100-101	32.53171027617742	34.0	32.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	2.0
19	22.0
20	99.0
21	629.0
22	2788.0
23	9395.0
24	24365.0
25	49884.0
26	88479.0
27	136205.0
28	192083.0
29	251198.0
30	316582.0
31	388965.0
32	479674.0
33	603050.0
34	793499.0
35	1083154.0
36	1636801.0
37	2696482.0
38	3176382.0
39	1520859.0
40	57562.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.082637599596275	22.140178814866065	15.39364240624612	24.38354117929154
2	13.939454286813135	22.91338719665789	40.812791570995536	22.33436694553344
3	16.977980940558822	27.812527552788275	31.513327387791723	23.696164118861184
4	12.1872918156661	19.630866111234536	39.28254186487709	28.89930020822227
5	12.631053183331172	38.496298644945085	36.088783661965536	12.78386450975821
6	28.555648692668083	39.418407879503356	19.239317624360563	12.786625803467993
7	25.015588724475524	33.1385078990397	24.5615446839877	17.284358692497076
8	23.746467043145252	37.31687089012339	21.864101264413417	17.072560802317945
9	25.441138878933998	16.409924341292644	21.65330277008099	36.49563400969236
10-11	23.61769303756448	27.20197442124061	29.704646694690712	19.4756858465042
12-13	24.547382874693305	24.881229206551506	29.580828952216372	20.99055896653882
14-15	21.737703600068137	26.088710372936774	27.160862237280114	25.012723789714975
16-17	21.285240085604546	29.047051630492117	27.84818747718509	21.819520806718252
18-19	21.37056810095756	28.34705671237655	29.38836587728304	20.89400930938285
20-21	22.244850479707583	27.575080339938395	29.09794393056127	21.082125249792753
22-23	21.95133066644108	27.561622444395372	28.93525397456228	21.55179291460126
24-25	21.70459026447261	27.71352600607262	28.98314234802928	21.598741381425494
26-27	21.66685634788959	27.825153626971723	28.882022576807557	21.62596744833113
28-29	21.619156943790593	27.885857915807645	28.920003377164573	21.57498176323719
30-31	21.85332006192056	27.749459764903612	28.85270502018861	21.544515152987223
32-33	21.690566311849267	27.891723595953682	28.7534795219421	21.664230570254954
34-35	21.79030529017175	27.920799711581196	28.800356811083848	21.488538187163204
36-37	21.7722806363092	27.893694516205386	28.74420283807504	21.589822009410373
38-39	21.63086945456988	27.963304349306874	28.808719616203682	21.59710657991957
40-41	21.80778922066366	27.831130073205664	28.70028951000582	21.66079119612485
42-43	21.724475653942182	27.988238316823207	28.470334052824462	21.816951976410152
44-45	21.812162437455054	28.04751555949281	28.331218620635156	21.809103382416982
46-47	21.89134131446139	28.018243177736274	28.074808670622424	22.015606837179913
48-49	21.836101953988145	28.14190901546274	28.064905634554805	21.95708339599431
50-51	21.868632090512282	28.218602162669626	27.93975814378698	21.97300760303111
52-53	21.961671705618315	28.114930563068995	27.858818306125453	22.06457942518723
54-55	21.938739716902145	28.05490044892561	27.873567316222104	22.13279251795014
56-57	21.95519338150007	28.074179896786745	27.87058533952748	22.100041382185704
58-59	21.92477666750888	28.10444268505656	27.88736610273016	22.083414544704397
60-61	21.884013933328482	27.935091823401585	27.993782770112425	22.187111473157508
62-63	22.07186873687408	28.012726401330788	27.97327380735043	21.942131054444708
64-65	21.811951546526778	27.952708920705604	27.97516837047709	22.26017116229053
66-67	21.903097799381037	27.995862025119568	27.81107120202598	22.289968973473417
68-69	21.856864162503996	28.252914643306674	27.835854568536494	22.054366625652836
70-71	21.874189414098943	28.066585641087478	27.880596531244016	22.17862841356956
72-73	21.887709174469673	28.051624917004204	27.597833670774364	22.462832237751762
74-75	21.831447635171926	28.094427328376714	27.575556668408858	22.498568368042502
76-77	21.95902803353898	27.802333957965057	27.76312448502346	22.475513523472497
78-79	21.949728863948444	27.765110805966316	27.737671384238787	22.547488945846453
80-81	22.22563473741079	27.80794882147446	27.453334813368013	22.51308162774674
82-83	22.237517670273306	28.02630163916876	27.547232245177018	22.188948445380912
84-85	22.049171357051854	27.76608762115635	27.504597535614234	22.680143486177563
86-87	21.94354873649685	28.030927264324728	27.55640653382286	22.46911746535556
88-89	22.241943895034744	27.706388949691945	27.55142574261365	22.500241412659655
90-91	22.31890650499867	27.93806328466342	27.607805108143236	22.135225102194667
92-93	22.036283599436118	27.86080985652205	27.63672107387474	22.466185470167098
94-95	22.20888870604569	27.569205160108552	27.736755034999533	22.48515109884622
96-97	22.134307786715503	27.916006034001516	27.798046568196266	22.151639611086715
98-99	22.381918605967936	28.311676923336016	27.957570664744836	21.348833805951212
100-101	25.97308640464316	35.57389562136241	28.855456003340542	9.597561970653896
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3123.0
1	2836.0
2	3908.0
3	5636.5
4	7533.0
5	11548.5
6	14891.0
7	17424.5
8	18963.0
9	18818.5
10	17790.5
11	16681.0
12	18577.0
13	24467.0
14	37794.5
15	56016.5
16	69971.0
17	76710.0
18	77655.5
19	74932.5
20	70665.5
21	67189.5
22	68009.5
23	75893.5
24	91536.0
25	114489.0
26	142328.5
27	171430.5
28	201922.0
29	233150.0
30	261552.5
31	291451.5
32	324121.5
33	355741.0
34	386407.5
35	415216.5
36	444578.0
37	469810.5
38	488940.5
39	504952.0
40	519705.0
41	535214.5
42	546775.5
43	556507.0
44	564661.5
45	569667.5
46	571257.0
47	570088.5
48	566615.5
49	557518.0
50	544789.5
51	526394.5
52	501805.0
53	474975.5
54	445041.0
55	410423.5
56	372359.0
57	331732.5
58	290060.5
59	248894.0
60	209270.5
61	171203.0
62	135238.0
63	104401.5
64	78139.0
65	58179.0
66	43964.0
67	33116.5
68	24794.5
69	17555.5
70	12247.5
71	8826.0
72	6194.5
73	4281.5
74	2967.0
75	2056.0
76	1389.0
77	899.5
78	594.5
79	428.5
80	295.5
81	203.5
82	161.5
83	113.5
84	74.5
85	57.5
86	48.0
87	41.0
88	33.0
89	24.5
90	18.5
91	14.5
92	11.0
93	6.0
94	2.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0038199130140660893
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.108023216483724E-4
20-21	0.0011289940477953154
22-23	0.002602530829809736
24-25	3.7029506332619543E-5
26-27	0.0078200341569905
28-29	1.5936989147688707E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.03465884094993684
38-39	0.014888716774229921
40-41	0.024282059364238707
42-43	0.0012465449736113355
44-45	0.005286016438189619
46-47	0.004279003116553713
48-49	0.002538202534080939
50-51	0.0022315708735047924
52-53	1.6239159295475443E-4
54-55	0.0025323956093669135
56-57	0.014286095137859368
58-59	0.010039416952176972
60-61	0.0034458469771812897
62-63	0.0010906861664311254
64-65	0.003063145912890937
66-67	3.0523663535563796E-4
68-69	4.947254973614566E-4
70-71	2.730387159051856E-4
72-73	0.0
74-75	0.0
76-77	0.00593765407339136
78-79	2.833179554643062E-4
80-81	0.0
82-83	1.4111867891116137E-4
84-85	0.0019804224621392365
86-87	0.005631009240822119
88-89	0.08054369286358516
90-91	0.003019329153827817
92-93	0.0013325849705355943
94-95	0.0
96-97	0.016641987146336308
98-99	4.065112179383876E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	326.0
20-21	1248.0
22-23	2422.0
24-25	5248.0
26-27	6323.0
28-29	11520.0
30-31	34862.0
32-33	44637.0
34-35	62967.0
36-37	96578.0
38-39	105107.0
40-41	111465.0
42-43	125835.0
44-45	148198.0
46-47	148885.0
48-49	135839.0
50-51	123136.0
52-53	112061.0
54-55	121118.0
56-57	129244.0
58-59	77466.0
60-61	135898.0
62-63	128841.0
64-65	136066.0
66-67	146499.0
68-69	148368.0
70-71	148477.0
72-73	142677.0
74-75	144961.0
76-77	144613.0
78-79	155370.0
80-81	158356.0
82-83	152480.0
84-85	150371.0
86-87	159640.0
88-89	168730.0
90-91	181493.0
92-93	187697.0
94-95	227915.0
96-97	289081.0
98-99	1128456.0
100-101	7667687.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.56012015744648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.99295690469319	76.9980820608945
2	7.092385684878927	12.136507428023885
3	1.6316066353781022	4.188013793179121
4	0.6106953855949082	2.090046822843938
5	0.2699463726576888	1.1548322040329337
6	0.14202431854148626	0.7290970655813409
7	0.07406894135271576	0.4436143265451251
8	0.04518642080946158	0.3092924475153978
9	0.032678847229336824	0.25164054859940055
>10	0.10523809280996771	1.4380707715576893
>50	0.0024920345061579105	0.14608197954648344
>100	6.920849078791391E-4	0.09534156361811126
>500	2.827664020233889E-5	0.019378988062046184
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	7.402932197802499E-6	2.2208796593407497E-5	0.0	2.9611728791209996E-5	0.0
3	7.402932197802499E-6	2.2208796593407497E-5	0.0	2.9611728791209996E-5	0.0
4	7.402932197802499E-6	5.182052538461749E-5	0.0	4.4417593186814994E-5	0.0
5	1.4805864395604998E-5	5.182052538461749E-5	0.0	4.4417593186814994E-5	0.0
6	1.4805864395604998E-5	5.182052538461749E-5	0.0	4.4417593186814994E-5	0.0
7	1.4805864395604998E-5	5.922345758241999E-5	0.0	4.4417593186814994E-5	7.402932197802499E-6
8	1.4805864395604998E-5	7.402932197802499E-5	0.0	4.4417593186814994E-5	7.402932197802499E-6
9	2.2208796593407497E-5	1.3325277956044497E-4	0.0	7.402932197802499E-5	7.402932197802499E-6
10-11	2.9611728791209996E-5	1.4065571175824747E-4	0.0	8.143225417582748E-5	1.4805864395604998E-5
12-13	2.9611728791209996E-5	1.4435717785714873E-4	0.0	1.0734251686813623E-4	1.4805864395604998E-5
14-15	3.331319489011125E-5	1.7767037274725998E-4	0.0	1.258498473626425E-4	1.8507330494506248E-5
16-17	4.4417593186814994E-5	1.9247623714286496E-4	0.0	1.4805864395604998E-4	2.2208796593407497E-5
18-19	5.182052538461749E-5	1.9987916934066747E-4	0.0	1.7026744054945747E-4	2.2208796593407497E-5
20-21	5.182052538461749E-5	2.0728210153846997E-4	0.0	2.2208796593407496E-4	2.9611728791209996E-5
22-23	8.513372027472874E-5	2.776099574175937E-4	0.0	4.6638472846155743E-4	3.7014660989012495E-5
24-25	1.258498473626425E-4	3.4053488109891494E-4	0.0	0.00106602223648356	4.4417593186814994E-5
26-27	1.4805864395604998E-4	3.7014660989012496E-4	0.0	0.0031388432518682594	4.4417593186814994E-5
28-29	1.5546157615385249E-4	3.7014660989012496E-4	0.0	0.017163698300605094	4.4417593186814994E-5
30-31	1.6656597445055622E-4	4.2566860137364373E-4	0.0	0.02583253190423182	4.4417593186814994E-5
32-33	1.8507330494506248E-4	4.367729996703474E-4	0.0	0.03188442897593536	4.4417593186814994E-5
34-35	1.92476237142865E-4	4.7378766065935994E-4	0.0	0.040175713037474164	5.182052538461749E-5
36-37	1.9987916934066747E-4	5.256081860439774E-4	0.0	0.05198338989296915	5.182052538461749E-5
38-39	1.9987916934066747E-4	5.589213809340887E-4	0.0	0.07389977066456345	5.922345758241999E-5
40-41	1.9987916934066747E-4	5.848316436263974E-4	0.0	0.11805826122445534	5.922345758241999E-5
42-43	1.9987916934066747E-4	5.959360419231011E-4	0.0	0.1372170497523682	5.922345758241999E-5
44-45	1.9987916934066747E-4	6.477565673077186E-4	0.0	0.16446354170638033	5.922345758241999E-5
46-47	1.9987916934066747E-4	6.958756265934348E-4	0.0	0.19594080941143655	5.922345758241999E-5
48-49	2.0358063543956872E-4	7.032785587912373E-4	0.0	0.22267649904380027	7.402932197802499E-5
50-51	2.1468503373627248E-4	7.550990841758548E-4	0.0	0.24812777993984525	7.402932197802499E-5
52-53	2.1468503373627248E-4	7.625020163736574E-4	0.0	0.2817111818551763	8.143225417582748E-5
54-55	2.1468503373627248E-4	7.6990494857146E-4	0.0	0.34875953877067356	8.883518637362999E-5
56-57	2.1838649983517373E-4	7.921137451648674E-4	0.0	0.4207493529282039	9.993958467033373E-5
58-59	2.2208796593407496E-4	7.958152112637687E-4	0.0	0.48242318106809656	1.1104398296703748E-4
60-61	2.2208796593407496E-4	8.106210756593736E-4	0.0	0.9298971192303676	1.1104398296703748E-4
62-63	2.2208796593407496E-4	8.291284061538798E-4	0.0	0.9411421732388294	1.2214838126374124E-4
64-65	2.2208796593407496E-4	9.438738552198186E-4	0.0	0.9527277621283904	1.258498473626425E-4
66-67	2.2949089813187746E-4	9.697841179121273E-4	0.0	0.9624441106380062	1.2955131346154372E-4
68-69	2.2949089813187746E-4	9.919929145055348E-4	0.0	0.9716829700208637	1.3325277956044497E-4
70-71	2.2949089813187746E-4	9.993958467033374E-4	0.0	0.9800778951331717	1.4065571175824747E-4
72-73	2.3319236423077872E-4	0.0010030973128022386	0.0	0.9865258490774577	1.4435717785714873E-4
74-75	2.3689383032967997E-4	0.0010660222364835598	0.0	0.9920484364970183	1.4805864395604998E-4
76-77	2.3689383032967997E-4	0.0011215442279670787	0.0	0.9970306098661395	1.4805864395604998E-4
78-79	2.5910262692308743E-4	0.0011326486262637824	0.0	1.0016093234304804	1.5176011005495123E-4
80-81	2.8131142351649495E-4	0.0011326486262637824	0.0	1.0041596335726233	1.6656597445055624E-4
82-83	2.8131142351649495E-4	0.0011326486262637824	0.0	1.0059141285035025	1.8507330494506248E-4
84-85	2.8131142351649495E-4	0.0011548574228571898	0.0	1.0075353706548211	1.8507330494506248E-4
86-87	2.8131142351649495E-4	0.0011770662194505973	0.0	1.0077833688834477	1.887747710439637E-4
88-89	2.8131142351649495E-4	0.0012140808804396099	0.0	1.0078203835444366	1.9617770324176621E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15895	0.0	47.19828	1
GTATCAA	27560	0.0	35.28172	1
GACCGAG	12545	0.0	27.807915	90-91
ACCGAGG	12925	0.0	27.374224	92-93
AGACCGA	12515	0.0	27.276194	90-91
TCAACGC	34745	0.0	27.229301	4
GAGACCG	12680	0.0	27.181356	88-89
ATCAACG	34980	0.0	27.117859	3
CGAGACC	12750	0.0	26.459513	88-89
CAACGCA	35760	0.0	26.433125	5
TATCAAC	36845	0.0	26.073933	2
AACGCAG	36645	0.0	25.90848	6
ACGAGAC	13440	0.0	25.573423	86-87
GCCCACG	13470	0.0	24.40153	82-83
CACGAGA	13755	0.0	24.250927	86-87
CCCACGA	13695	0.0	23.838163	84-85
CCACGAG	14320	0.0	23.389626	84-85
ATCTCCG	13880	0.0	22.812935	74-75
CGAGCCC	14295	0.0	22.32413	80-81
TCCGAGC	14135	0.0	22.290611	78-79
>>END_MODULE