FastQCFastQC Report
Fri 27 May 2016
SRR2139806_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139806_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17630492
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA185600.045.4726261
TATCTCG213700.035.45726894-95
CCGTCTT89450.034.0472292-93
GTATCAA319800.033.4726561
TATGCCG88850.032.53005288-89
TGCCGTC91750.031.96716990-91
GCCGTCT88650.031.7955690-91
ATGCCGT94150.029.23252588-89
CGTATGC104050.027.83970586-87
TCAACGC374300.027.6381364
ATCAACG376200.027.5439853
CTCGTAT104950.027.27922684-85
CAACGCA384400.026.9564195
AACGCAG399000.026.0771336
TCGTATG106850.025.65453784-85
TATCAAC409950.025.5906622
TCTCGTA109450.024.71781382-83
CTATCTC351450.024.38789794-95
ACGCAGA458850.022.6571467
CGCAGAG468700.022.1354088
Produced by FastQC (version 0.11.5)