##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139806_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17630492
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06843649059822	33.0	31.0	34.0	30.0	34.0
2	32.2302429223189	34.0	31.0	34.0	30.0	34.0
3	32.28424884569302	34.0	31.0	34.0	30.0	34.0
4	35.91669081044363	37.0	35.0	37.0	35.0	37.0
5	35.792404772368236	37.0	35.0	37.0	35.0	37.0
6	35.80462842443648	37.0	35.0	37.0	35.0	37.0
7	35.72555014346735	37.0	35.0	37.0	33.0	37.0
8	35.70710715276692	37.0	35.0	37.0	33.0	37.0
9	37.34728174347035	39.0	37.0	39.0	34.0	39.0
10-11	37.26000213153439	39.0	37.0	39.0	33.5	39.0
12-13	37.23855752295512	39.0	37.0	39.0	33.0	39.0
14-15	38.4683691186837	40.0	38.0	41.0	34.0	41.0
16-17	38.45067015146259	40.0	38.0	41.0	33.0	41.0
18-19	38.48479568806134	40.0	38.0	41.0	33.5	41.0
20-21	38.45854173760901	40.0	38.0	41.0	34.0	41.0
22-23	38.391356406716966	40.0	38.0	41.0	34.0	41.0
24-25	38.30475299516989	40.0	38.0	41.0	33.5	41.0
26-27	38.16278129699226	40.0	38.0	41.0	33.0	41.0
28-29	38.066355971791985	40.0	37.5	41.0	33.0	41.0
30-31	37.9608577525008	40.0	37.0	41.0	33.0	41.0
32-33	38.127732535330225	40.0	37.5	41.0	33.0	41.0
34-35	38.11709227521509	40.0	38.0	41.0	33.0	41.0
36-37	38.0394800506232	40.0	37.0	41.0	33.0	41.0
38-39	37.91825862175517	40.0	37.0	41.0	33.0	41.0
40-41	37.87236082392434	40.0	37.0	41.0	33.0	41.0
42-43	37.79550622634345	40.0	37.0	41.0	33.0	41.0
44-45	37.79831741681591	40.0	37.0	41.0	33.0	41.0
46-47	37.83755006329581	40.0	37.0	41.0	33.0	41.0
48-49	37.76288693614127	40.0	37.0	41.0	33.0	41.0
50-51	37.704149043922996	40.0	37.0	41.0	32.0	41.0
52-53	37.56915332608262	40.0	36.5	41.0	32.0	41.0
54-55	37.46217501342506	39.5	36.0	41.0	32.0	41.0
56-57	37.304067634403765	39.0	36.0	41.0	31.0	41.0
58-59	37.15694009352509	39.0	35.5	41.0	31.0	41.0
60-61	37.01430482561587	39.0	35.0	40.0	31.0	41.0
62-63	36.80067489538136	38.5	35.0	40.0	31.0	41.0
64-65	36.609094565212985	38.0	35.0	40.0	31.0	41.0
66-67	36.363070493254355	38.0	35.0	40.0	31.0	41.0
68-69	36.07779545867864	37.0	34.5	40.0	30.0	41.0
70-71	35.79207958374332	37.0	34.0	39.0	30.0	41.0
72-73	35.474263920426196	36.0	34.0	39.0	30.0	40.5
74-75	35.11827732073321	36.0	34.0	39.0	29.5	40.0
76-77	33.995633705114386	35.0	32.5	37.0	28.5	39.0
78-79	34.310561809094544	35.0	33.0	37.0	29.0	39.0
80-81	34.165885542923874	35.0	33.0	37.0	29.0	39.0
82-83	33.900767310041246	35.0	33.0	36.0	29.0	38.5
84-85	33.693967533320304	35.0	33.0	36.0	29.0	37.0
86-87	33.517758441972354	35.0	33.0	35.5	29.0	37.0
88-89	33.30294059129065	35.0	33.0	35.0	29.0	36.5
90-91	33.1964558930016	35.0	33.0	35.0	29.0	36.0
92-93	33.14550498648662	35.0	33.0	35.0	29.0	36.0
94-95	33.105310401331195	35.0	33.0	35.0	29.0	36.0
96-97	33.04532103158788	34.0	33.0	35.0	29.0	35.0
98-99	33.12243397754064	34.0	33.0	35.0	29.0	35.0
100-101	32.60727681134043	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	5.0
19	17.0
20	88.0
21	695.0
22	3343.0
23	11348.0
24	29912.0
25	62385.0
26	110723.0
27	171636.0
28	242214.0
29	317585.0
30	399995.0
31	492228.0
32	603456.0
33	762841.0
34	1001529.0
35	1366186.0
36	2072850.0
37	3505650.0
38	4434925.0
39	1957041.0
40	83839.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.91102936889112	22.25770557055356	15.414272046406873	24.416993014148442
2	13.727872213415088	22.907966831321154	41.10316691590596	22.2609940393578
3	16.77215247311306	27.89369122540653	31.680335409811594	23.65382089166882
4	12.053146333068868	19.736732247744417	39.800607946732285	28.409513472454428
5	12.5424350040827	38.4885685549785	36.42982850393511	12.539167937003686
6	28.271167928836014	39.75510155927583	19.43989424685369	12.533836265034465
7	24.738816137405582	33.269599055999116	24.888448944022663	17.10313586257264
8	23.40883056468305	37.49577720236055	22.134691419842394	16.96070081311401
9	25.158163481767836	16.711065125125266	21.96956273256583	36.161208660541064
10-11	23.35875822410401	27.288132401523452	30.086585218381877	19.266524155990655
12-13	24.255037806091856	24.946584020457287	29.868919710238373	20.929458463212484
14-15	21.506943765381024	26.287851184187033	27.30687833328758	24.898326717144364
16-17	21.17138307881595	29.21555167036745	28.02718154433807	21.585883706478526
18-19	21.208763718177927	28.639400476228737	29.41644779511019	20.735388010483145
20-21	22.01255178897012	27.898366488607213	29.150692229321045	20.938389493101617
22-23	21.73062514781676	27.820738045357686	28.983603877123336	21.46503292970222
24-25	21.55863723418673	27.98152294083914	28.966301657773585	21.493538167200537
26-27	21.546710459466777	28.100516624101896	28.896101278986663	21.45667163744466
28-29	21.515010468788372	28.139210452814247	28.93226081620291	21.413518262194476
30-31	21.73534737115409	27.986862983420796	28.884131028263848	21.393658617161265
32-33	21.554571943825568	28.13091555077152	28.81121074598902	21.503301759413898
34-35	21.667027503051195	28.169892843172196	28.796112881590197	21.366966772186412
36-37	21.641346586241653	28.123971564977808	28.77423205227398	21.46044979650656
38-39	21.513027079663	28.224210752811135	28.827432038100586	21.435330129425274
40-41	21.67244624267474	27.975044442528823	28.80041877516405	21.552090539632385
42-43	21.687179514374687	28.09517365205706	28.586653429906132	21.63099340366212
44-45	21.603879348065174	28.14676989046908	28.4697690177222	21.779581743743538
46-47	21.659725961372168	28.12116823214624	28.282358991092334	21.936746815389267
48-49	21.591642010829958	28.315565540268352	28.3251100271428	21.767682421758895
50-51	21.62684889580814	28.380024631382927	28.219482917632423	21.773643555176513
52-53	21.685263552994467	28.27896274295413	27.92199258856706	22.113781115484336
54-55	21.65022868465881	27.997469590770212	28.148581353225126	22.20372037134585
56-57	21.674171278093056	27.694922812238946	28.414014509855757	22.216891399812244
58-59	21.523612731618226	27.46188226702861	28.604958885837778	22.40954611551539
60-61	21.60276280665303	27.274809097021553	28.862347055514736	22.260081040810686
62-63	21.368807921180252	27.782560721718426	28.88020416284271	21.968427194258616
64-65	20.91909968959376	28.014560005088118	28.67919877149957	22.387141533818546
66-67	21.015874716944886	28.652397716961236	28.14687098920099	22.18485657689289
68-69	21.113447414937127	28.945356602609195	27.70674608376102	22.23444989869266
70-71	21.114480221968527	28.56042204457357	27.69082744111514	22.634270292342766
72-73	21.000854737933906	28.17990163724869	27.607810072661003	23.2114335521564
74-75	21.47886417447471	27.785403168127893	27.69110726254796	23.04462539484944
76-77	21.866991871155147	27.627158494550535	27.592522234014705	22.913327400279616
78-79	22.06573078340642	27.49342472408293	26.929015564515602	23.511828927995047
80-81	21.982225079782168	27.92950428436971	26.812953185336045	23.275317450512077
82-83	21.935000103618798	28.06025394315508	26.903567535161958	23.10117841806416
84-85	21.927088588785324	28.0662669062848	26.894395688193214	23.112248816736667
86-87	22.273625343263866	28.358326430865795	26.81094433284955	22.55710389302079
88-89	22.434876139213454	28.025037140090003	27.18011903307377	22.359967687622774
90-91	22.184477042315482	28.011428136837484	27.436830504021465	22.36726431682557
92-93	21.95969679498532	27.4875642330228	27.35366557698527	23.199073395006614
94-95	21.781568132156405	27.471771853637755	28.030505304901077	22.716154709304764
96-97	21.32377860613962	27.770539065637035	28.31934920138572	22.58633312683763
98-99	21.570313930430217	27.828868868046875	28.73242717130805	21.86839003021486
100-101	22.224585720852275	30.829307271492123	26.026003671686972	20.920103335968637
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3278.0
1	2963.5
2	4120.5
3	5972.0
4	8059.5
5	12320.5
6	15742.5
7	18110.0
8	19506.0
9	19353.5
10	18406.5
11	17527.5
12	19463.5
13	25562.0
14	39335.0
15	58317.0
16	73878.5
17	82540.5
18	84775.0
19	82679.5
20	78914.0
21	76174.0
22	77613.0
23	87105.0
24	105107.0
25	131423.0
26	165504.0
27	203235.0
28	244148.5
29	287477.5
30	329810.5
31	371632.0
32	415043.0
33	459122.5
34	501441.0
35	541769.0
36	585035.5
37	622892.0
38	653228.5
39	680889.5
40	704735.5
41	725156.0
42	737387.5
43	748165.5
44	758904.0
45	763827.0
46	763139.5
47	756623.5
48	744913.5
49	725285.5
50	698587.0
51	663649.0
52	622304.5
53	578641.5
54	531327.5
55	483625.0
56	435371.5
57	384883.0
58	334191.0
59	283498.5
60	232774.0
61	185975.0
62	145550.0
63	111626.0
64	83487.5
65	62137.0
66	46791.5
67	35729.5
68	27209.0
69	19779.0
70	14164.5
71	10308.0
72	7338.5
73	5040.5
74	3435.5
75	2331.0
76	1582.0
77	1035.5
78	699.0
79	487.5
80	340.5
81	230.0
82	169.0
83	119.0
84	82.5
85	65.0
86	48.0
87	35.0
88	23.5
89	16.5
90	13.0
91	10.5
92	9.0
93	6.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004809848755213411
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.586911584770295E-4
20-21	0.0010975739248653643
22-23	0.002464851962664612
24-25	1.7022327875398148E-5
26-27	0.007830207054343936
28-29	1.8739608567624105E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.03719842322123924
38-39	0.016835217991481247
40-41	0.02540671822109606
42-43	0.0013330729299882403
44-45	0.005279958100901359
46-47	0.004336943729469335
48-49	0.0025708800997646317
50-51	0.0021809608393555725
52-53	2.454029584062845E-4
54-55	0.00251006853931093
56-57	0.014018883830377537
58-59	0.009590365947347104
60-61	0.003210793810307168
62-63	0.001062633626178492
64-65	0.0028578801766946786
66-67	3.129435295955486E-4
68-69	6.010926312829934E-4
70-71	2.388145637626707E-4
72-73	0.0
74-75	0.0
76-77	0.006290008211634573
78-79	3.542246795556303E-4
80-81	3.487097113004403E-6
82-83	1.2377657027642878E-4
84-85	0.001828729381614084
86-87	0.0051572621251942475
88-89	0.0770553724795995
90-91	0.0028228318296060627
92-93	0.0013184846232017445
94-95	0.0
96-97	0.01851966565233855
98-99	5.8416806081156133E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	399.0
20-21	1629.0
22-23	3099.0
24-25	5885.0
26-27	7455.0
28-29	13147.0
30-31	39629.0
32-33	53025.0
34-35	75739.0
36-37	115413.0
38-39	82053.0
40-41	132590.0
42-43	146507.0
44-45	169582.0
46-47	173486.0
48-49	158820.0
50-51	145792.0
52-53	25530.0
54-55	24927.0
56-57	24813.0
58-59	25253.0
60-61	166718.0
62-63	163741.0
64-65	171945.0
66-67	184534.0
68-69	188092.0
70-71	187132.0
72-73	181575.0
74-75	186267.0
76-77	187500.0
78-79	199229.0
80-81	201299.0
82-83	196057.0
84-85	193703.0
86-87	205269.0
88-89	215881.0
90-91	234347.0
92-93	243334.0
94-95	300612.0
96-97	382933.0
98-99	1603004.0
100-101	1.0612547E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.11874725239299
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.1042117848006	74.95334670249338
2	7.577557632591985	12.74829310572893
3	1.8133661213837404	4.576142593221932
4	0.6918411023608939	2.3278722731325194
5	0.31486099956499364	1.3242856421021758
6	0.15697398859961545	0.7922673163326642
7	0.10039495345183817	0.5911568400381653
8	0.06042463346472382	0.40662755761900726
9	0.041670976213991226	0.31547792843146877
>10	0.13566601150968846	1.7284353735747504
>50	0.002484637576429617	0.13761545212989137
>100	5.129458587819411E-4	0.07787111384237311
>500	3.4212622740569287E-5	0.02060810135276745
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.671991456619588E-6	0.0
2	0.0	2.2687965826478352E-5	0.0	5.671991456619588E-6	0.0
3	0.0	2.2687965826478352E-5	0.0	1.1343982913239176E-5	0.0
4	0.0	7.373588893605465E-5	0.0	2.8359957283097942E-5	0.0
5	1.1343982913239176E-5	7.940788039267424E-5	0.0	3.403194873971753E-5	0.0
6	1.1343982913239176E-5	7.940788039267424E-5	0.0	3.403194873971753E-5	1.1343982913239176E-5
7	1.1343982913239176E-5	8.507987184929383E-5	0.0	3.970394019633712E-5	1.1343982913239176E-5
8	1.1343982913239176E-5	9.6423854762533E-5	0.0	4.5375931652956705E-5	1.1343982913239176E-5
9	1.1343982913239176E-5	1.3045580350225054E-4	0.0	6.239190602281547E-5	1.1343982913239176E-5
10-11	2.2687965826478352E-5	1.3045580350225054E-4	0.0	9.92598504908428E-5	1.1343982913239176E-5
12-13	3.403194873971753E-5	1.3045580350225054E-4	0.0	1.417997864154897E-4	1.1343982913239176E-5
14-15	3.970394019633712E-5	1.5597976505703868E-4	0.0	1.985197009816856E-4	1.1343982913239176E-5
16-17	5.1047923109576294E-5	1.588157607853485E-4	0.0	2.864355685592892E-4	1.7015974369858766E-5
18-19	5.6719914566195884E-5	1.7299573942689746E-4	0.0	4.055473891483006E-4	2.2687965826478352E-5
20-21	5.6719914566195884E-5	1.9568370525337582E-4	0.0	5.445111798354805E-4	3.970394019633712E-5
22-23	6.522790175112527E-5	2.0702768816661498E-4	0.0	8.990106458742048E-4	5.1047923109576294E-5
24-25	7.940788039267424E-5	2.3538764544971292E-4	0.0	0.0016392055309630612	6.239190602281547E-5
26-27	8.507987184929383E-5	2.4956762409126187E-4	0.0	0.0037123184083575206	6.239190602281547E-5
28-29	8.507987184929383E-5	2.4956762409126187E-4	0.0	0.016746554775669335	6.239190602281547E-5
30-31	1.0493184194746238E-4	2.835995728309794E-4	0.0	0.02740422672265754	6.522790175112527E-5
32-33	1.1343982913239177E-4	2.89271564287599E-4	0.0	0.04080147054319301	7.373588893605465E-5
34-35	1.3896379068717993E-4	3.431554831254851E-4	0.0	0.05854629581522739	9.075186330591341E-5
36-37	1.6448775224196807E-4	3.913674105067516E-4	0.0	0.08116619774422631	9.075186330591341E-5
38-39	1.6448775224196807E-4	3.970394019633712E-4	0.0	0.1303281836944766	9.92598504908428E-5
40-41	1.6448775224196807E-4	4.2539935924646913E-4	0.0	0.4446103943100397	1.0209584621915259E-4
42-43	1.6448775224196807E-4	4.395793378880181E-4	0.0	0.4864441672983374	1.0209584621915259E-4
44-45	1.7015974369858765E-4	4.906272609975944E-4	0.0	0.5547434524232222	1.0209584621915259E-4
46-47	1.7866773088351704E-4	5.274952054656217E-4	0.0	0.6315875926775044	1.0776783767577219E-4
48-49	1.8150372661182682E-4	5.274952054656217E-4	0.0	0.6960497755819861	1.1343982913239177E-4
50-51	1.8717571806844643E-4	6.06903085858296E-4	0.0	0.7576277508307765	1.2478381204563093E-4
52-53	1.92847709525066E-4	6.21083064499845E-4	0.0	0.9749274155253297	1.3329179923056032E-4
54-55	1.985197009816856E-4	6.267550559564645E-4	0.0	1.7174875210515963	1.3612779495887013E-4
56-57	1.985197009816856E-4	6.295910516847743E-4	0.0	2.5359048403186932	1.3896379068717993E-4
58-59	1.985197009816856E-4	6.295910516847743E-4	0.0	3.380869915598498	1.417997864154897E-4
60-61	2.1553567535154437E-4	6.409350345980135E-4	0.0	4.022034665850505	1.417997864154897E-4
62-63	2.1553567535154437E-4	6.551150132395624E-4	0.0	4.039217963968333	1.417997864154897E-4
64-65	2.1553567535154437E-4	7.089989320774485E-4	0.0	4.050006091718824	1.417997864154897E-4
66-67	2.1553567535154437E-4	7.146709235340682E-4	0.0	4.0586133387542445	1.417997864154897E-4
68-69	2.2120766680816395E-4	7.28850902175617E-4	0.0	4.066140071417179	1.417997864154897E-4
70-71	2.2120766680816395E-4	7.373588893605465E-4	0.0	4.073462612387674	1.503077736004191E-4
72-73	2.2120766680816395E-4	7.401948850888563E-4	0.0	4.079350139519645	1.7015974369858765E-4
74-75	2.2687965826478354E-4	7.713908381002639E-4	0.0	4.084732859411978	1.7299573942689743E-4
76-77	2.2687965826478354E-4	8.167667697532207E-4	0.0	4.08989720763323	1.7866773088351704E-4
78-79	2.3255164972140312E-4	8.337827441230795E-4	0.0	4.094414948828428	1.8150372661182682E-4
80-81	2.3255164972140312E-4	8.366187398513893E-4	2.835995728309794E-6	4.097089292800224	1.8433972234013662E-4
82-83	2.3255164972140312E-4	8.422907313080088E-4	5.671991456619588E-6	4.098858954134689	2.0135569670999537E-4
84-85	2.3255164972140312E-4	8.451267270363187E-4	5.671991456619588E-6	4.100407407802346	2.0419169243830518E-4
86-87	2.3255164972140312E-4	8.67814692862797E-4	5.671991456619588E-6	4.100671155405079	2.0419169243830518E-4
88-89	2.3255164972140312E-4	8.905026586892754E-4	5.671991456619588E-6	4.10070235135809	2.0419169243830518E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18560	0.0	45.472626	1
TATCTCG	21370	0.0	35.457268	94-95
CCGTCTT	8945	0.0	34.04722	92-93
GTATCAA	31980	0.0	33.472656	1
TATGCCG	8885	0.0	32.530052	88-89
TGCCGTC	9175	0.0	31.967169	90-91
GCCGTCT	8865	0.0	31.79556	90-91
ATGCCGT	9415	0.0	29.232525	88-89
CGTATGC	10405	0.0	27.839705	86-87
TCAACGC	37430	0.0	27.638136	4
ATCAACG	37620	0.0	27.543985	3
CTCGTAT	10495	0.0	27.279226	84-85
CAACGCA	38440	0.0	26.956419	5
AACGCAG	39900	0.0	26.077133	6
TCGTATG	10685	0.0	25.654537	84-85
TATCAAC	40995	0.0	25.590662	2
TCTCGTA	10945	0.0	24.717813	82-83
CTATCTC	35145	0.0	24.387897	94-95
ACGCAGA	45885	0.0	22.657146	7
CGCAGAG	46870	0.0	22.135408	8
>>END_MODULE