FastQCFastQC Report
Fri 27 May 2016
SRR2139805_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139805_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15154766
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162450.046.122971
GTATCAA281600.033.881731
TCAACGC342900.027.2852844
ATCAACG344050.027.1330813
CAACGCA351850.026.5196515
AACGCAG361350.025.8689046
TATCAAC371150.025.491962
ACGCAGA419500.022.2630147
CGCAGAG427850.021.8383358
GTACATG388650.019.2031231
TACATGG389450.018.7541052
ACATGGG394700.017.983013
GCAGAGT518550.017.9214389
AGAGTAC457400.017.83504710-11
GAGTACT286050.017.37391912-13
CAGAGTA503550.016.45888510-11
CATGGGG288250.016.1636564
AGTACTT323450.015.12492312-13
GTACTTT331650.014.9291614-15
TATGCCG45500.014.54816742-43
Produced by FastQC (version 0.11.5)