##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139805_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15154766
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03624879460362	33.0	31.0	34.0	30.0	34.0
2	32.20027184847328	33.0	31.0	34.0	30.0	34.0
3	32.25152687939887	34.0	31.0	34.0	30.0	34.0
4	35.89398740963734	37.0	35.0	37.0	35.0	37.0
5	35.768612065669636	37.0	35.0	37.0	35.0	37.0
6	35.780254013819814	37.0	35.0	37.0	33.0	37.0
7	35.69955458236703	37.0	35.0	37.0	33.0	37.0
8	35.68247982185934	37.0	35.0	37.0	33.0	37.0
9	37.317308957459325	39.0	37.0	39.0	34.0	39.0
10-11	37.22507490382893	39.0	37.0	39.0	33.0	39.0
12-13	37.200603394338124	39.0	37.0	39.0	33.0	39.0
14-15	38.4181110087744	40.0	38.0	41.0	33.0	41.0
16-17	38.39832324035884	40.0	38.0	41.0	33.0	41.0
18-19	38.432318288517294	40.0	38.0	41.0	33.5	41.0
20-21	38.4052743100233	40.0	38.0	41.0	34.0	41.0
22-23	38.33550682980035	40.0	38.0	41.0	34.0	41.0
24-25	38.24790770407438	40.0	38.0	41.0	33.0	41.0
26-27	38.09854376183684	40.0	37.5	41.0	33.0	41.0
28-29	38.00202009000995	40.0	37.0	41.0	33.0	41.0
30-31	37.893125718784134	40.0	37.0	41.0	33.0	41.0
32-33	38.05881451208849	40.0	37.0	41.0	33.0	41.0
34-35	38.044352304522036	40.0	37.0	41.0	33.0	41.0
36-37	37.964842193463724	40.0	37.0	41.0	33.0	41.0
38-39	37.837836807528994	40.0	37.0	41.0	33.0	41.0
40-41	37.79123335885257	40.0	37.0	41.0	33.0	41.0
42-43	37.713484939670515	40.0	37.0	41.0	33.0	41.0
44-45	37.71562603416064	40.0	37.0	41.0	32.5	41.0
46-47	37.754953874677675	40.0	37.0	41.0	33.0	41.0
48-49	37.67932410735598	40.0	37.0	41.0	32.0	41.0
50-51	37.61361071973319	40.0	36.5	41.0	32.0	41.0
52-53	37.47877558172995	39.5	36.0	41.0	32.0	41.0
54-55	37.357230811226	39.0	36.0	41.0	31.0	41.0
56-57	37.19185522604387	39.0	36.0	41.0	31.0	41.0
58-59	37.033339040732386	39.0	35.0	40.5	31.0	41.0
60-61	36.87900425275246	39.0	35.0	40.0	31.0	41.0
62-63	36.65536722985301	38.0	35.0	40.0	31.0	41.0
64-65	36.43854272578326	38.0	35.0	40.0	31.0	41.0
66-67	36.18408621840361	37.5	35.0	40.0	30.0	41.0
68-69	35.894820911582	37.0	34.0	40.0	30.0	41.0
70-71	35.61061233046225	36.5	34.0	39.0	30.0	41.0
72-73	35.29509531529633	36.0	34.0	39.0	29.5	40.0
74-75	34.956945140229465	35.5	34.0	38.5	29.0	40.0
76-77	33.87575674493978	35.0	32.5	37.0	28.5	39.0
78-79	34.23513037392243	35.0	33.0	37.0	29.0	39.0
80-81	34.12702675556352	35.0	33.0	36.5	29.0	39.0
82-83	33.87851879693331	35.0	33.0	36.0	29.0	38.0
84-85	33.666111914904235	35.0	33.0	36.0	29.0	37.0
86-87	33.48327064292372	35.0	33.0	35.5	29.0	37.0
88-89	33.27987500026273	35.0	33.0	35.0	29.0	36.5
90-91	33.161052795188596	35.0	33.0	35.0	29.0	36.0
92-93	33.09914049807983	35.0	33.0	35.0	29.0	36.0
94-95	33.068056739793654	34.0	33.0	35.0	29.0	35.5
96-97	33.02493502102759	34.0	33.0	35.0	29.0	35.0
98-99	33.10938183591976	34.0	33.0	35.0	29.0	35.0
100-101	32.590496003011616	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	3.0
18	10.0
19	25.0
20	132.0
21	682.0
22	3023.0
23	10142.0
24	26241.0
25	54592.0
26	96547.0
27	149263.0
28	211071.0
29	277016.0
30	349344.0
31	430376.0
32	531941.0
33	671448.0
34	884553.0
35	1209738.0
36	1837591.0
37	3043760.0
38	3611724.0
39	1690903.0
40	64640.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.810342964055	22.221280091028788	15.476919933966649	24.491457010949556
2	13.853357243135967	22.755385892006657	40.915503483363494	22.47575338149388
3	16.91958820083398	27.737432567418065	31.518394939255412	23.82458429249254
4	12.205434250848874	19.66410434842742	39.250833698125064	28.879627702598643
5	12.694527912869127	38.31378854678456	36.25505006147901	12.736633478867306
6	28.691845192462885	39.43552807083923	19.13403347831303	12.738593258384853
7	24.97768028882795	33.03275682382691	24.584015352002137	17.405547535343008
8	23.60714114622423	37.49791319773595	21.842798496525777	17.052147159514046
9	25.177960517503205	16.621761101425122	21.73008148063784	36.470196900433834
10-11	23.653258651436783	27.150204760667368	29.734216945349072	19.462319642546774
12-13	24.496343262574953	24.743915544456442	29.598936070672423	21.160805122296182
14-15	21.696527679807133	26.052365308708826	27.007774319973006	25.24333269151104
16-17	21.398479527826428	29.01076796566836	27.74954096948775	21.841211537017465
18-19	21.45871533843926	28.32397561044493	29.186943125966863	21.03036592514895
20-21	22.203480493263008	27.59326548916902	28.922186857757954	21.281067159810014
22-23	21.957390640790315	27.600084146266006	28.75884422628746	21.683680986656217
24-25	21.755618352544012	27.78277053405469	28.780069428888496	21.681541684512805
26-27	21.768865194125215	27.81981442472305	28.707491924395985	21.70382845675575
28-29	21.69449835095788	27.828175866684095	28.80583701412326	21.671488768234763
30-31	21.902194038600378	27.707637347858917	28.65245106619319	21.73771754734752
32-33	21.832620893413054	27.896946585639803	28.61193843383818	21.65849408710896
34-35	21.899432182734067	27.93113342840864	28.605617683900597	21.563816704956697
36-37	21.833631229082027	27.917207997660746	28.528225587358406	21.720935185898817
38-39	21.72722760122962	27.941396291611515	28.658178225608783	21.673197881550085
40-41	21.93715396862287	27.793789327300882	28.575057993175484	21.69399871090076
42-43	21.84614563604484	27.946475562696072	28.352756227667008	21.85462257359208
44-45	21.90510487170681	28.04431251997954	28.181489265822073	21.869093342491574
46-47	22.019941885022064	27.98745158932813	27.975094797958434	22.017511727691375
48-49	21.92713958701535	28.106819506939225	27.946160490896826	22.019880415148602
50-51	21.934065224403813	28.153845600059046	27.899085437317904	22.01300373821923
52-53	22.082495691679053	28.068370621162504	27.853539926531507	21.995593760626935
54-55	22.006106008491546	28.01435795608957	27.849789490169563	22.129746545249322
56-57	22.076764892637936	28.06810494422261	27.80882808259725	22.04630208054221
58-59	22.006295676735572	28.17724079530605	27.838937240948052	21.977526287010328
60-61	22.1114139563624	28.15901434414157	27.798469510510497	21.93110218898553
62-63	22.06937418472209	28.233632704139527	27.750302628130253	21.94669048300813
64-65	22.01698891297637	28.177384722533493	27.7284300270032	22.07719633748694
66-67	22.085496215126255	28.150590390745478	27.656595739243862	22.107317654884405
68-69	22.027146347411037	28.085792649229536	27.74392505441617	22.143135948943257
70-71	22.046667095300286	27.929373977689977	27.810572921188754	22.213386005820983
72-73	22.076235819959898	27.96771250952187	27.741921343248492	22.21413032726974
74-75	21.974815824621494	28.030915791149834	27.7539540430842	22.240314341144472
76-77	22.0806186642901	27.954563931759047	27.732928586464823	22.23188881748603
78-79	22.025282241313068	27.972208484262893	27.701819067778523	22.300690206645516
80-81	22.067818149372577	27.98302999016473	27.696976751029112	22.25217510943358
82-83	22.089900983300968	27.911856187808503	27.804384092691652	22.193858736198877
84-85	22.114911406378102	27.949696539880726	27.75235123498012	22.18304081876105
86-87	22.044722796760748	27.90565678884806	27.82339321784515	22.226227196546038
88-89	22.071712160816524	27.86712332238986	27.770979237052494	22.290185279741117
90-91	22.096131013863697	27.870280111599694	27.8471883278888	22.186400546647807
92-93	22.138215870355868	27.790876762603244	27.87239308262066	22.198514284420227
94-95	21.97403685482746	27.798358210523244	27.94273923887402	22.28486569577527
96-97	21.940281185114692	27.82843455698784	27.998121222748175	22.233163035149296
98-99	22.11672959095176	28.44594153097025	28.053186382318522	21.384142495759466
100-101	22.69357717942232	31.114053158602793	25.26827922073697	20.924090441237915
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3032.0
1	2677.0
2	3680.5
3	5306.5
4	7065.0
5	10875.0
6	13954.0
7	16193.0
8	17588.5
9	17231.5
10	16037.5
11	15133.0
12	17073.5
13	22785.0
14	35605.5
15	53389.0
16	67580.0
17	75040.5
18	76850.5
19	74758.0
20	70918.5
21	68219.0
22	69784.0
23	78001.0
24	93895.5
25	117737.0
26	147262.5
27	179851.5
28	216448.5
29	255139.5
30	291071.0
31	327212.0
32	365388.0
33	402090.5
34	436928.5
35	469872.5
36	504009.0
37	534855.5
38	559321.0
39	578425.5
40	594162.5
41	609459.5
42	621181.0
43	631365.5
44	639684.0
45	644346.5
46	645174.0
47	643205.5
48	641018.0
49	629406.5
50	608155.0
51	585126.5
52	554813.0
53	520316.5
54	485464.5
55	447412.0
56	409035.0
57	368074.5
58	324507.0
59	278721.0
60	231649.5
61	189076.5
62	150384.5
63	116469.0
64	87518.5
65	65054.0
66	49220.5
67	37464.5
68	28564.5
69	20947.0
70	14995.5
71	10900.5
72	7742.5
73	5282.5
74	3646.5
75	2466.5
76	1652.0
77	1095.0
78	726.5
79	503.5
80	370.5
81	274.0
82	196.5
83	130.5
84	87.0
85	63.5
86	49.5
87	37.0
88	24.5
89	18.0
90	15.0
91	10.0
92	6.0
93	4.5
94	3.0
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0038997632823891838
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.410839072012066E-4
20-21	0.0012042924414020168
22-23	0.0026893083329951077
24-25	2.6404080724271855E-5
26-27	0.008082710428644491
28-29	2.2464438051256248E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.03557433243894454
38-39	0.01542025289955466
40-41	0.024561650540201933
42-43	0.0012315011845502018
44-45	0.005359825236543731
46-47	0.004493494174588064
48-49	0.0026042978961084164
50-51	0.002131212479924103
52-53	1.7263825383887234E-4
54-55	0.0024685386563352034
56-57	0.013698135777242128
58-59	0.009386244517971674
60-61	0.003204157324799207
62-63	0.0010340003165631739
64-65	0.0030123857666571534
66-67	2.6328140468992456E-4
68-69	3.9185522642198287E-4
70-71	1.7452047821308163E-4
72-73	0.0
74-75	0.0
76-77	0.005551267372845446
78-79	2.9986260045761535E-4
80-81	0.0
82-83	1.0701396035637873E-4
84-85	0.0017010366473157838
86-87	0.005064757389540435
88-89	0.07350784041942304
90-91	0.002792184516633134
92-93	0.0013726054389744705
94-95	4.711098037290602E-6
96-97	0.016542051008695848
98-99	6.0454834973666123E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	392.0
20-21	1249.0
22-23	2629.0
24-25	5382.0
26-27	7477.0
28-29	12628.0
30-31	34629.0
32-33	45447.0
34-35	63503.0
36-37	102204.0
38-39	114770.0
40-41	117064.0
42-43	131912.0
44-45	154973.0
46-47	155423.0
48-49	142169.0
50-51	130064.0
52-53	125866.0
54-55	137551.0
56-57	145191.0
58-59	145992.0
60-61	143022.0
62-63	137489.0
64-65	144258.0
66-67	155619.0
68-69	153383.0
70-71	152487.0
72-73	146088.0
74-75	149729.0
76-77	150801.0
78-79	160942.0
80-81	163783.0
82-83	159324.0
84-85	157568.0
86-87	166341.0
88-89	177463.0
90-91	192709.0
92-93	199105.0
94-95	243739.0
96-97	319360.0
98-99	1225032.0
100-101	8880009.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.0849579625883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.3283538442195	75.1117087785841
2	7.360036003968321	12.377366359936252
3	1.800600111710932	4.542101541019364
4	0.6745861714486426	2.2689019947361
5	0.3150249631225091	1.3244430390661053
6	0.16685104076911145	0.8417797649450905
7	0.10325897657518103	0.607776869320879
8	0.06269581834924881	0.4217420200261326
9	0.04104526213408027	0.31061602269968014
>10	0.14307344696384755	1.875783979938249
>50	0.0036952501123014994	0.20723376372559188
>100	7.509716977215917E-4	0.09347480774582266
>500	2.8138928553897175E-5	0.017071058256702132
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3197168468322111E-5
2	0.0	2.6394336936644223E-5	0.0	6.598584234161056E-6	1.3197168468322111E-5
3	0.0	3.2992921170805276E-5	0.0	6.598584234161056E-6	1.3197168468322111E-5
4	0.0	7.258442657577161E-5	0.0	3.959150540496633E-5	1.3197168468322111E-5
5	0.0	9.238017927825478E-5	0.0	3.959150540496633E-5	1.3197168468322111E-5
6	2.6394336936644223E-5	9.897876351241583E-5	0.0	3.959150540496633E-5	1.9795752702483166E-5
7	2.6394336936644223E-5	9.897876351241583E-5	0.0	4.619008963912739E-5	1.9795752702483166E-5
8	2.6394336936644223E-5	1.1217593198073793E-4	0.0	4.619008963912739E-5	1.9795752702483166E-5
9	2.6394336936644223E-5	1.9795752702483167E-4	0.0	5.9387258107449495E-5	3.2992921170805276E-5
10-11	2.6394336936644223E-5	1.9795752702483167E-4	0.0	8.248230292701319E-5	3.959150540496633E-5
12-13	2.6394336936644223E-5	2.1775327972731483E-4	0.0	9.238017927825478E-5	3.959150540496633E-5
14-15	3.2992921170805276E-5	3.7941859346426066E-4	0.0	9.238017927825478E-5	4.619008963912739E-5
16-17	4.619008963912739E-5	3.9591505404966334E-4	0.0	9.238017927825478E-5	5.9387258107449495E-5
18-19	4.619008963912739E-5	4.2560868310338805E-4	0.0	9.56794713953353E-5	7.258442657577161E-5
20-21	4.619008963912739E-5	4.7179877274251543E-4	0.0	1.0887663986365741E-4	7.918301080993266E-5
22-23	4.619008963912739E-5	5.212881544987233E-4	0.0	1.319716846832211E-4	9.56794713953353E-5
24-25	4.619008963912739E-5	5.641789520207702E-4	0.0	1.4186956103446268E-4	1.1547522409781846E-4
26-27	4.619008963912739E-5	5.93872581074495E-4	0.0	1.7486248220526798E-4	1.1877451621489899E-4
28-29	4.619008963912739E-5	6.070697495428171E-4	0.0	3.7611930134718013E-4	1.2207380833197953E-4
30-31	4.619008963912739E-5	6.499605470648639E-4	0.0	0.0011184600276902988	1.286723925661406E-4
32-33	4.619008963912739E-5	6.697562997673471E-4	0.0	0.003101334590055696	1.3857026891738217E-4
34-35	5.9387258107449495E-5	7.720343553968435E-4	0.0	0.005697877486198071	1.3857026891738217E-4
36-37	5.9387258107449495E-5	8.578159504409372E-4	0.0	0.009544852094713965	1.3857026891738217E-4
38-39	5.9387258107449495E-5	8.974074558459036E-4	0.0	0.014925997537672306	1.4846814526862375E-4
40-41	6.598584234161055E-5	9.633932981875141E-4	0.0	0.020973599988280914	1.550667295027848E-4
42-43	6.598584234161055E-5	9.897876351241583E-4	0.0	0.02656590012673241	1.6166531373694584E-4
44-45	6.928513445869107E-5	0.0010854671065194936	0.0	0.033022614799859004	1.6496460585402637E-4
46-47	7.258442657577161E-5	0.0011778472857977484	0.0	0.040122691435816295	1.8476035855650956E-4
48-49	7.588371869285214E-5	0.0011844458700319095	0.0	0.04707100063438789	2.1775327972731483E-4
50-51	8.908088716117426E-5	0.0013329140153005331	0.0	0.053771862924178446	2.1775327972731483E-4
52-53	9.238017927825478E-5	0.0013395125995346942	0.0	0.06009330662050473	2.507462008981201E-4
54-55	9.238017927825478E-5	0.0013560090601200969	0.0	0.06754640751298964	2.8373912206892536E-4
56-57	9.238017927825478E-5	0.0013791041049396606	0.0	0.07494671973160126	2.8373912206892536E-4
58-59	9.238017927825478E-5	0.001412097026110466	0.0	0.08230084186057376	2.870384141860059E-4
60-61	1.0227805562949637E-4	0.001445089947281271	0.0	0.08984632293233694	3.101334590055696E-4
62-63	1.0887663986365741E-4	0.0014714842842179154	0.0	0.09707177266874328	3.2333062747389173E-4
64-65	1.1217593198073793E-4	0.001662843227008586	0.0	0.10400688469884656	3.266299195909722E-4
66-67	1.2207380833197953E-4	0.0016925368560623108	0.0	0.11125542948007247	3.2992921170805275E-4
68-69	1.2537310044906006E-4	0.0017123326087647938	0.0	0.11881410772030396	3.332285038251333E-4
70-71	1.3197168468322113E-4	0.0017552234062868407	0.0	0.12591088506414416	3.431263801763749E-4
72-73	1.5176743738570426E-4	0.001781617743223485	0.0	0.1316186604266935	3.827178855813412E-4
74-75	1.5176743738570426E-4	0.001808012080160129	0.0	0.1368216440953295	3.8931646981550227E-4
76-77	1.550667295027848E-4	0.0018542021697992566	0.0	0.1414241565986568	3.926157619325828E-4
78-79	1.5836602161986533E-4	0.0018805965067359009	0.0	0.1454756873184317	3.9591505404966334E-4
80-81	1.6166531373694584E-4	0.0018937936752042228	0.0	0.1477488995871002	4.157108067521465E-4
82-83	1.6496460585402637E-4	0.0019003922594383839	0.0	0.14924017962402059	4.421051436887907E-4
84-85	1.6496460585402637E-4	0.0019102901357896255	0.0	0.15063578018954565	4.4540443580587126E-4
86-87	1.682638979711069E-4	0.0019531809333116724	0.0	0.1508997235589121	4.553023121571128E-4
88-89	1.7816177432234849E-4	0.002025765359887444	0.0	0.15095581152490245	4.619008963912739E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16245	0.0	46.12297	1
GTATCAA	28160	0.0	33.88173	1
TCAACGC	34290	0.0	27.285284	4
ATCAACG	34405	0.0	27.133081	3
CAACGCA	35185	0.0	26.519651	5
AACGCAG	36135	0.0	25.868904	6
TATCAAC	37115	0.0	25.49196	2
ACGCAGA	41950	0.0	22.263014	7
CGCAGAG	42785	0.0	21.838335	8
GTACATG	38865	0.0	19.203123	1
TACATGG	38945	0.0	18.754105	2
ACATGGG	39470	0.0	17.98301	3
GCAGAGT	51855	0.0	17.921438	9
AGAGTAC	45740	0.0	17.835047	10-11
GAGTACT	28605	0.0	17.373919	12-13
CAGAGTA	50355	0.0	16.458885	10-11
CATGGGG	28825	0.0	16.163656	4
AGTACTT	32345	0.0	15.124923	12-13
GTACTTT	33165	0.0	14.92916	14-15
TATGCCG	4550	0.0	14.548167	42-43
>>END_MODULE