FastQCFastQC Report
Fri 27 May 2016
SRR2139804_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139804_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13271175
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139950.044.6541751
GTATCAA237050.034.1722641
TCAACGC283150.027.8034254
ATCAACG286850.027.5046863
CAACGCA292600.026.934825
AACGCAG302800.026.1267916
TATCAAC308350.025.7134112
ACGCAGA352550.022.3790057
CGCAGAG360100.021.9217268
GTACATG363900.020.8793891
TACATGG364750.020.3714892
ACATGGG372000.019.24623
AGAGTAC385250.017.97667710-11
GCAGAGT442250.017.7913939
GAGTACT229800.017.3441412-13
CATGGGG271450.017.2302044
CAGAGTA425950.016.47073210-11
AGTACTT257800.015.72692712-13
GTACTTT260050.015.27797814-15
GTGGTAT79550.014.1999731
Produced by FastQC (version 0.11.5)