##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139804_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13271175
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04989739039686	33.0	31.0	34.0	30.0	34.0
2	32.21725762790408	33.0	31.0	34.0	30.0	34.0
3	32.271438813820176	34.0	31.0	34.0	30.0	34.0
4	35.90463828560772	37.0	35.0	37.0	35.0	37.0
5	35.78059222337133	37.0	35.0	37.0	35.0	37.0
6	35.791356304170506	37.0	35.0	37.0	33.0	37.0
7	35.70931209934312	37.0	35.0	37.0	33.0	37.0
8	35.69320674318589	37.0	35.0	37.0	33.0	37.0
9	37.33456962175542	39.0	37.0	39.0	34.0	39.0
10-11	37.24309347137687	39.0	37.0	39.0	33.0	39.0
12-13	37.21530034077615	39.0	37.0	39.0	33.0	39.0
14-15	38.44328776464782	40.0	38.0	41.0	33.0	41.0
16-17	38.42420772840386	40.0	38.0	41.0	33.0	41.0
18-19	38.45231842696671	40.0	38.0	41.0	33.5	41.0
20-21	38.423281246768724	40.0	38.0	41.0	34.0	41.0
22-23	38.35699088337688	40.0	38.0	41.0	34.0	41.0
24-25	38.269634503614	40.0	38.0	41.0	33.0	41.0
26-27	38.12287024289519	40.0	38.0	41.0	33.0	41.0
28-29	38.02717096803799	40.0	37.0	41.0	33.0	41.0
30-31	37.92168768993708	40.0	37.0	41.0	33.0	41.0
32-33	38.090203141839815	40.0	37.5	41.0	33.0	41.0
34-35	38.07669618648691	40.0	37.5	41.0	33.0	41.0
36-37	37.99441882532492	40.0	37.0	41.0	33.0	41.0
38-39	37.86764246659337	40.0	37.0	41.0	33.0	41.0
40-41	37.82192023132246	40.0	37.0	41.0	33.0	41.0
42-43	37.74362044886248	40.0	37.0	41.0	33.0	41.0
44-45	37.74140506521444	40.0	37.0	41.0	32.5	41.0
46-47	37.77775317860906	40.0	37.0	41.0	33.0	41.0
48-49	37.70085486754094	40.0	37.0	41.0	32.0	41.0
50-51	37.635267427302864	40.0	37.0	41.0	32.0	41.0
52-53	37.49269840216648	39.5	36.0	41.0	32.0	41.0
54-55	37.368493918469234	39.0	36.0	41.0	31.5	41.0
56-57	37.20008720119324	39.0	36.0	41.0	31.0	41.0
58-59	37.035681116923506	39.0	35.0	40.5	31.0	41.0
60-61	36.8776829522103	39.0	35.0	40.0	31.0	41.0
62-63	36.65059052819004	38.0	35.0	40.0	31.0	41.0
64-65	36.425164424447416	38.0	35.0	40.0	31.0	41.0
66-67	36.161926061600205	37.5	35.0	40.0	30.0	41.0
68-69	35.86484657035431	37.0	34.0	39.5	30.0	41.0
70-71	35.57457763109552	36.5	34.0	39.0	30.0	41.0
72-73	35.25138889784324	36.0	34.0	39.0	29.5	40.0
74-75	34.91018228162106	35.5	34.0	38.5	29.0	40.0
76-77	33.8320301085525	35.0	32.5	37.0	28.5	39.0
78-79	34.17449437991027	35.0	33.0	37.0	29.0	39.0
80-81	34.061978595663206	35.0	33.0	36.5	29.0	39.0
82-83	33.820167599624455	35.0	33.0	36.0	29.0	37.5
84-85	33.61480915345443	35.0	33.0	36.0	29.0	37.0
86-87	33.43961936404595	35.0	33.0	35.0	29.0	37.0
88-89	33.24315950619179	35.0	33.0	35.0	29.0	36.0
90-91	33.1330797048834	35.0	33.0	35.0	29.0	36.0
92-93	33.07766331516677	35.0	33.0	35.0	29.0	36.0
94-95	33.053753329417916	34.5	33.0	35.0	29.0	35.5
96-97	33.01774214019676	34.0	33.0	35.0	29.0	35.0
98-99	33.10257997653872	34.0	33.0	35.0	29.0	35.0
100-101	32.584109243526626	34.0	32.0	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	20.0
20	83.0
21	530.0
22	2590.0
23	9315.0
24	23411.0
25	49340.0
26	87260.0
27	134961.0
28	188690.0
29	247722.0
30	308609.0
31	380340.0
32	467424.0
33	588779.0
34	777164.0
35	1067273.0
36	1626263.0
37	2726531.0
38	3222610.0
39	1316897.0
40	45362.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.93444062036707	22.431954970076124	15.507858196429481	24.12574621312732
2	14.252436785910449	22.767719789608005	40.788161430138686	22.191681994342858
3	17.361966819064627	27.581031822728587	31.11672478134001	23.940276576866783
4	12.458829003460508	19.384967796747464	38.83527268685704	29.32093051293499
5	12.931952144403189	38.31785052943692	35.9729338208561	12.777263505303788
6	29.369501946888654	39.01243107712769	18.876188430941497	12.741878545042168
7	25.616073934674212	32.700668930972576	24.215451909872336	17.467805224480877
8	23.910836832458315	37.643230535352	21.460202280506437	16.985730351683255
9	25.45384263262296	16.617255065960627	21.2290999101436	36.69980239127281
10-11	24.129773738949265	27.00096638014343	29.4272587016598	19.442001179247505
12-13	24.941657389040532	24.457615847880838	29.305332798339258	21.29539396473937
14-15	22.07983467929554	25.760473356729907	26.59423148289432	25.565460481080237
16-17	21.882263627749616	28.742722479358456	27.302431774127005	22.072582118764917
18-19	21.943151367535265	28.122284042425417	28.661519593474605	21.273044996564717
20-21	22.734428737726887	27.361920510438516	28.367943506455536	21.535707245379065
22-23	22.43864679804402	27.344777688718423	28.235923496007985	21.980652017229573
24-25	22.272454505072186	27.492628163742467	28.271906955098263	21.96301037608708
26-27	22.267658958329037	27.57739240383858	28.215130976081966	21.939817661750418
28-29	22.164873832200833	27.614745353735593	28.269034253229385	21.951346560834185
30-31	22.460699500772925	27.469135091729953	28.15348045374423	21.916684953752892
32-33	22.19652676007487	27.652299216339454	28.15329455152589	21.997879472059786
34-35	22.35587517512781	27.6163954652415	28.17655051314476	21.85117884648593
36-37	22.281727110354748	27.625083440957667	28.118182643101363	21.97500680558623
38-39	22.17206635505344	27.64316212275009	28.228858686242635	21.95591283595383
40-41	22.367294993189642	27.486426412872948	28.16163769242056	21.98464090151685
42-43	22.23783152947529	27.612750142126703	27.952720234852258	22.19669809354575
44-45	22.345852139341726	27.672392910292253	27.824938602552013	22.156816347814004
46-47	22.430960171850845	27.642939245831965	27.622599704056327	22.303500878260863
48-49	22.286852218828002	27.790457217751385	27.661988698544015	22.260701864876598
50-51	22.333796024825975	27.84304138050004	27.589962327915092	22.233200266758892
52-53	22.474295553079696	27.72636930854383	27.53707429589902	22.26226084247745
54-55	22.405870346208413	27.657174595403035	27.59586204733418	22.341093011054372
56-57	22.432042848837423	27.77781840963226	27.531259776756244	22.258878964774077
58-59	22.377477807356158	27.864753376562767	27.540701588603728	22.21706722747735
60-61	22.438600336625335	27.863841616579748	27.52960228271066	22.16795576408426
62-63	22.418359447196284	27.930497185901565	27.486072066010138	22.165071300892013
64-65	22.407897177002827	27.855705066271145	27.48189091899502	22.254506837731004
66-67	22.449582101927927	27.86244555577336	27.381446138378664	22.30652620392005
68-69	22.380044212842417	27.847887302085457	27.429417231972575	22.342651253099554
70-71	22.376168701810876	27.688004368592978	27.510658720741038	22.42516820885511
72-73	22.3828702365752	27.75539302349675	27.459311235954466	22.402425503973586
74-75	22.290591068231038	27.837451562404315	27.435526710738582	22.436430658626065
76-77	22.39377465423781	27.751470702045015	27.431140861009823	22.423613782707346
78-79	22.352737785245424	27.807642170046325	27.399225868886663	22.44039417582159
80-81	22.375969675037467	27.811731408287155	27.391649536465255	22.42064938021012
82-83	22.351972398143324	27.79490725326026	27.500925205140312	22.352195143456104
84-85	22.37967516508177	27.818164746092144	27.441221968962516	22.36093811986357
86-87	22.321229732865028	27.81620755013452	27.48028195133526	22.38228076566519
88-89	22.299812484134847	27.81804362140195	27.4467930355654	22.435350858897802
90-91	22.346224565526445	27.829620877926832	27.50255147716225	22.321603079384474
92-93	22.364174970489387	27.791218266022533	27.489537146197918	22.35506961729016
94-95	22.186607792716327	27.839057481796758	27.55474582254369	22.41958890294322
96-97	22.186747630694995	27.872331900259994	27.589498927296415	22.351421541748596
98-99	22.356856632400948	28.473691833165347	27.704399262575436	21.46505227185827
100-101	22.905940460029786	31.196132798068426	24.86966650822256	21.028260233679227
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3456.0
1	2798.5
2	2929.0
3	3907.0
4	4955.5
5	7478.5
6	9645.0
7	11271.5
8	12326.0
9	12136.5
10	11202.0
11	10183.0
12	11053.0
13	14479.0
14	23169.5
15	36328.0
16	47448.0
17	53508.0
18	54796.0
19	52788.0
20	49174.0
21	45488.0
22	44039.5
23	47018.0
24	55158.5
25	69040.0
26	87268.0
27	107692.5
28	133202.0
29	163978.0
30	196240.5
31	231320.0
32	269589.0
33	309165.0
34	347256.0
35	382573.0
36	420647.5
37	455189.5
38	483169.5
39	506429.0
40	526736.5
41	545926.0
42	557307.0
43	565197.0
44	573090.0
45	579252.0
46	582455.0
47	581804.0
48	575414.5
49	562925.5
50	547786.0
51	526459.0
52	497639.0
53	466542.5
54	434939.0
55	401193.5
56	366096.0
57	329602.5
58	291001.0
59	249687.0
60	206219.5
61	165866.0
62	130622.0
63	99895.5
64	73810.0
65	54376.5
66	40769.0
67	30736.0
68	23117.5
69	16470.0
70	11569.0
71	8282.0
72	5760.5
73	3842.5
74	2474.5
75	1657.5
76	1112.0
77	716.5
78	480.5
79	324.5
80	214.5
81	146.0
82	109.5
83	81.0
84	59.5
85	44.5
86	38.0
87	30.5
88	18.0
89	10.0
90	9.0
91	8.5
92	6.5
93	4.5
94	3.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0039031962128447555
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.500643311537976E-4
20-21	0.0011717607174189766
22-23	0.0026565116828862092
24-25	7.5378570023299515E-6
26-27	0.007819497642142462
28-29	1.546476697972007E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.03529098460820161
38-39	0.01549450352371854
40-41	0.02406877531394406
42-43	0.0013429723918934316
44-45	0.005442612042075446
46-47	0.004545030280674493
48-49	0.0024536413405031455
50-51	0.0022446417465900917
52-53	1.4883558679797774E-4
54-55	0.0025813010551564465
56-57	0.014171925680771266
58-59	0.009839261570835638
60-61	0.0035040528964842086
62-63	0.0011320668328652587
64-65	0.002943465044512931
66-67	3.2015868942912416E-4
68-69	4.440232594040354E-4
70-71	2.3075954154784184E-4
72-73	0.0
74-75	0.0
76-77	0.00578713698893468
78-79	2.493709956685618E-4
80-81	4.586296768990617E-6
82-83	1.1137253235012425E-4
84-85	0.001549268135122472
86-87	0.004898477044007138
88-89	0.07408440635874469
90-91	0.0026114494633032045
92-93	0.0011102757057450175
94-95	0.0
96-97	0.016756976457788637
98-99	2.1496120299597878E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	342.0
20-21	1094.0
22-23	2247.0
24-25	4458.0
26-27	5362.0
28-29	8197.0
30-31	20985.0
32-33	27882.0
34-35	39833.0
36-37	66391.0
38-39	75379.0
40-41	78902.0
42-43	89854.0
44-45	105766.0
46-47	105881.0
48-49	96980.0
50-51	89952.0
52-53	89035.0
54-55	97840.0
56-57	103596.0
58-59	105673.0
60-61	105266.0
62-63	101344.0
64-65	107107.0
66-67	114873.0
68-69	114405.0
70-71	114524.0
72-73	110851.0
74-75	113776.0
76-77	114892.0
78-79	124269.0
80-81	127955.0
82-83	125299.0
84-85	124748.0
86-87	133268.0
88-89	141996.0
90-91	153747.0
92-93	162633.0
94-95	209131.0
96-97	285100.0
98-99	1138686.0
100-101	8331656.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.72813776378312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.0557277292016	74.56470239964554
2	7.452976734097604	12.480477254855893
3	1.902377192112445	4.778474988596091
4	0.7058964240134004	2.3641357214702343
5	0.3390890745202035	1.4195648372810659
6	0.18220523701001484	0.9153423111394369
7	0.11204211656565197	0.656675443980833
8	0.06371830196863984	0.42680118122436883
9	0.04143833261300444	0.3122598979550793
>10	0.14067278084698664	1.8195910178572798
>50	0.003255111204080203	0.17841118834727412
>100	5.851347290437759E-4	0.07657832007054316
>500	1.5831117375265836E-5	0.006985437576423132
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.2745894768172374E-5	0.0	7.535127824024625E-6	0.0
3	0.0	6.0281022592197E-5	0.0	7.535127824024625E-6	0.0
4	0.0	1.1302691736036937E-4	0.0	7.535127824024625E-6	7.535127824024625E-6
5	0.0	1.2809717300841863E-4	0.0	7.535127824024625E-6	7.535127824024625E-6
6	0.0	1.2809717300841863E-4	0.0	7.535127824024625E-6	1.507025564804925E-5
7	0.0	1.3563230083244324E-4	0.0	7.535127824024625E-6	1.507025564804925E-5
8	0.0	1.3563230083244324E-4	0.0	7.535127824024625E-6	1.507025564804925E-5
9	0.0	2.109835790726895E-4	0.0	2.2605383472073873E-5	1.507025564804925E-5
10-11	0.0	2.109835790726895E-4	0.0	2.6372947384086187E-5	2.2605383472073873E-5
12-13	0.0	2.109835790726895E-4	0.0	4.5210766944147746E-5	2.2605383472073873E-5
14-15	0.0	2.8633485731293577E-4	0.0	4.5210766944147746E-5	2.6372947384086187E-5
16-17	0.0	3.089402407850096E-4	0.0	5.2745894768172374E-5	3.01405112960985E-5
18-19	0.0	3.3908075208110807E-4	0.0	6.404858650420932E-5	3.01405112960985E-5
20-21	0.0	3.6168613555318197E-4	0.0	7.535127824024625E-5	3.01405112960985E-5
22-23	0.0	4.257347220573913E-4	0.0	8.665396997628318E-5	3.7675639120123125E-5
24-25	0.0	4.897833085616007E-4	0.0	1.3563230083244324E-4	4.5210766944147746E-5
26-27	0.0	5.349940755057484E-4	0.0	1.8837819560061562E-4	4.5210766944147746E-5
28-29	0.0	5.500643311537976E-4	0.0	3.089402407850096E-4	4.5210766944147746E-5
30-31	0.0	5.990426620099576E-4	0.0	8.627721358508195E-4	4.897833085616006E-5
32-33	0.0	6.0281022592197E-4	0.0	0.0026222244827605696	5.2745894768172374E-5
34-35	0.0	6.894641958982532E-4	0.0	0.0049166709051760675	5.2745894768172374E-5
36-37	7.535127824024625E-6	7.83653293698561E-4	0.0	0.008266035222955014	5.2745894768172374E-5
38-39	1.507025564804925E-5	8.477018802027702E-4	0.0	0.013793051481877077	5.2745894768172374E-5
40-41	1.8837819560061562E-5	9.569612336511274E-4	0.0	0.020194142568385996	5.2745894768172374E-5
42-43	2.2605383472073873E-5	0.001002172000595275	0.0	0.026015028812445017	5.651345868018469E-5
44-45	2.2605383472073873E-5	0.0010888259705715583	0.0	0.03307544358355609	6.0281022592197E-5
46-47	2.2605383472073873E-5	0.0011604096848997923	0.0	0.04086299818968554	7.535127824024625E-5
48-49	2.2605383472073873E-5	0.0011679448127238168	0.0	0.04836045037459004	9.042153388829549E-5
50-51	2.2605383472073873E-5	0.0012432960909640632	0.0	0.05591064845426272	9.042153388829549E-5
52-53	2.2605383472073873E-5	0.0012508312187880877	0.0	0.06329130615789483	1.0172422562433243E-4
54-55	2.2605383472073873E-5	0.0012508312187880877	0.0	0.07109393101967233	1.1679448127238168E-4
56-57	2.2605383472073873E-5	0.001265901474436137	0.0	0.07812420527948731	1.20562045184394E-4
58-59	2.2605383472073873E-5	0.0012809717300841864	0.0	0.08382076191444993	1.20562045184394E-4
60-61	2.2605383472073873E-5	0.0013299500609403464	0.0	0.0900862207001264	1.20562045184394E-4
62-63	2.2605383472073873E-5	0.0013600905722364449	0.0	0.0956584477259926	1.2809717300841863E-4
64-65	2.2605383472073873E-5	0.0014957228730688881	0.0	0.10141905294745943	1.2809717300841863E-4
66-67	2.2605383472073873E-5	0.0015371660761010235	0.0	0.1073115229058467	1.2809717300841863E-4
68-69	2.2605383472073873E-5	0.0015635390234851098	0.0	0.11332455490941834	1.2809717300841863E-4
70-71	2.2605383472073873E-5	0.0015710741513091343	0.0	0.11931498152951792	1.2809717300841863E-4
72-73	2.2605383472073873E-5	0.0015786092791331588	0.0	0.12431453884075826	1.4316742865646786E-4
74-75	2.2605383472073873E-5	0.001650192993461393	0.0	0.1286698427230445	1.4316742865646786E-4
76-77	2.2605383472073873E-5	0.001774522602557799	0.0	0.13279155764278594	1.469349925684802E-4
78-79	2.2605383472073873E-5	0.00180843067776591	0.0	0.13670605654736676	1.507025564804925E-4
80-81	2.2605383472073873E-5	0.0018121982416779221	0.0	0.13891008143589395	1.544701203925048E-4
82-83	2.2605383472073873E-5	0.0018498738807980453	0.0	0.14034552328637065	1.5823768430451711E-4
84-85	2.2605383472073873E-5	0.0018724792642701193	0.0	0.14171691655034313	1.5823768430451711E-4
86-87	2.2605383472073873E-5	0.0019101549033902422	0.0	0.14199194871592002	1.5823768430451711E-4
88-89	2.2605383472073873E-5	0.0019968088733665255	0.0	0.14205976486633626	1.6200524821652942E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13995	0.0	44.654175	1
GTATCAA	23705	0.0	34.172264	1
TCAACGC	28315	0.0	27.803425	4
ATCAACG	28685	0.0	27.504686	3
CAACGCA	29260	0.0	26.93482	5
AACGCAG	30280	0.0	26.126791	6
TATCAAC	30835	0.0	25.713411	2
ACGCAGA	35255	0.0	22.379005	7
CGCAGAG	36010	0.0	21.921726	8
GTACATG	36390	0.0	20.879389	1
TACATGG	36475	0.0	20.371489	2
ACATGGG	37200	0.0	19.2462	3
AGAGTAC	38525	0.0	17.976677	10-11
GCAGAGT	44225	0.0	17.791393	9
GAGTACT	22980	0.0	17.34414	12-13
CATGGGG	27145	0.0	17.230204	4
CAGAGTA	42595	0.0	16.470732	10-11
AGTACTT	25780	0.0	15.726927	12-13
GTACTTT	26005	0.0	15.277978	14-15
GTGGTAT	7955	0.0	14.199973	1
>>END_MODULE