FastQCFastQC Report
Fri 27 May 2016
SRR2139803_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139803_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9397010
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104900.045.613451
GTATCAA174650.032.0940741
ATCAACG202150.026.9647143
TCAACGC211950.025.717944
CAACGCA215050.025.3278435
AACGCAG218350.025.0022686
ACGCAGA224350.024.3707317
TATCAAC229550.024.0553862
CGCAGAG263450.020.7537428
GTACATG252350.019.9018021
TACATGG257100.019.0318872
ACATGGG250600.019.0096823
GCAGAGT316200.017.2124889
GAGTACT170850.017.12249412-13
AGAGTAC280800.017.05417310-11
CTTATAC67750.016.9647791
CAGAGTA308200.016.37570210-11
CATGGGG194300.016.2023834
GTACTTT194950.014.98546414-15
AGTACTT189100.014.78734412-13
Produced by FastQC (version 0.11.5)